Restriction map for
pF1KB3350
(Linear) (Six-base) MAPSORT of: KIBB3350.seq Check: 586 from: 1 to: 1905
KIBB3350.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 44 1905
Size: 44 1861
AflIII A'CryG_T
Cuts at: 0 1392 1905
Size: 1392 513
AleI CACnn'nnGTG
Cuts at: 0 1036 1905
Size: 1036 869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1486 1905
Size: 1486 419
AlwNI CAG_nnn'CTG
Cuts at: 0 426 1278 1905
Size: 426 852 627
Fragments arranged by size:
852 627 426
ApaI G_GGCC'C
Cuts at: 0 356 1905
Size: 356 1549
ApaLI G'TGCA_C
Cuts at: 0 1471 1905
Size: 1471 434
AvaI C'yCGr_G
Cuts at: 0 62 521 685 1063 1905
Size: 62 459 164 378 842
Fragments arranged by size:
842 459 378 164 62
BanI G'GyrC_C
Cuts at: 0 214 1775 1905
Size: 214 1561 130
Fragments arranged by size:
1561 214 130
BanII G_rGCy'C
Cuts at: 0 356 1051 1135 1905
Size: 356 695 84 770
Fragments arranged by size:
770 695 356 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 1124 1905
Size: 1124 781
BbvCI CC'TCA_GC
Cuts at: 0 455 1905
Size: 455 1450
BfrBI ATG'CAT
Cuts at: 0 1628 1905
Size: 1628 277
BglII A'GATC_T
Cuts at: 0 1479 1905
Size: 1479 426
Bme1580I G_kGCm'C
Cuts at: 0 356 1475 1780 1905
Size: 356 1119 305 125
Fragments arranged by size:
1119 356 305 125
BmgBI CAC'GTC
Cuts at: 0 1896 1905
Size: 1896 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 359 1138 1905
Size: 359 779 767
Fragments arranged by size:
779 767 359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 481 1632 1686 1905
Size: 448 33 1151 54 219
Fragments arranged by size:
1151 448 219 54 33
Bpu10I CC'TnA_GC
Cuts at: 0 455 1905
Size: 455 1450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 855 1905
Size: 292 563 1050
Fragments arranged by size:
1050 563 292
BsaWI w'CCGG_w
Cuts at: 0 1620 1905
Size: 1620 285
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 586 616 1630 1660 1905
Size: 259 30 297 30 1014 30 245
Fragments arranged by size:
1014 297 259 245 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 295 1081 1471 1905
Size: 82 213 786 390 434
Fragments arranged by size:
786 434 390 213 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 607 625 1905
Size: 358 249 18 1280
Fragments arranged by size:
1280 358 249 18
BsiHKAI G_wGCw'C
Cuts at: 0 1475 1905
Size: 1475 430
BsmI GAATG_Cn'
Cuts at: 0 1630 1693 1905
Size: 1630 63 212
Fragments arranged by size:
1630 212 63
Bsp1286I G_dGCh'C
Cuts at: 0 356 1051 1135 1475 1780 1905
Size: 356 695 84 340 305 125
Fragments arranged by size:
695 356 340 305 125 84
BspEI T'CCGG_A
Cuts at: 0 1620 1905
Size: 1620 285
BspHI T'CATG_A
Cuts at: 0 1811 1905
Size: 1811 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 44 419 1905
Size: 44 375 1486
Fragments arranged by size:
1486 375 44
BsrBI CCG'CTC
Cuts at: 0 201 1234 1767 1905
Size: 201 1033 533 138
Fragments arranged by size:
1033 533 201 138
BsrFI r'CCGG_y
Cuts at: 0 229 323 935 1905
Size: 229 94 612 970
Fragments arranged by size:
970 612 229 94
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1558 1905
Size: 1558 347
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1591 1905
Size: 1591 314
BstYI r'GATC_y
Cuts at: 0 1479 1905
Size: 1479 426
BtgI C'CryG_G
Cuts at: 0 161 168 507 1025 1193 1436 1590 1905
Size: 161 7 339 518 168 243 154 315
Fragments arranged by size:
518 339 315 243 168 161 154 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 210 1673 1905
Size: 210 1463 232
Fragments arranged by size:
1463 232 210
BtsI GCAGTG_nn'
Cuts at: 0 1552 1868 1905
Size: 1552 316 37
Fragments arranged by size:
1552 316 37
DraIII CAC_nnn'GTG
Cuts at: 0 1558 1905
Size: 1558 347
EaeI y'GGCC_r
Cuts at: 0 2 903 1905
Size: 2 901 1002
Fragments arranged by size:
1002 901 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 481 1632 1686 1905
Size: 448 33 1151 54 219
Fragments arranged by size:
1151 448 219 54 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 88 926 1905
Size: 88 838 979
Fragments arranged by size:
979 838 88
EcoO109I rG'GnC_Cy
Cuts at: 0 239 353 1003 1905
Size: 239 114 650 902
Fragments arranged by size:
902 650 239 114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 333 1905
Size: 333 1572
FspI TGC'GCA
Cuts at: 0 1879 1905
Size: 1879 26
HaeII r_GCGC'y
Cuts at: 0 29 1905
Size: 29 1876
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1309 1341 1791 1823 1905
Size: 1309 32 450 32 82
Fragments arranged by size:
1309 450 82 32 32
MscI TGG'CCA
Cuts at: 0 4 905 1905
Size: 4 901 1000
Fragments arranged by size:
1000 901 4
MslI CAynn'nnrTG
Cuts at: 0 1036 1441 1747 1905
Size: 1036 405 306 158
Fragments arranged by size:
1036 405 306 158
MspA1I CmG'CkG
Cuts at: 0 11 459 856 1278 1905
Size: 11 448 397 422 627
Fragments arranged by size:
627 448 422 397 11
NcoI C'CATG_G
Cuts at: 0 507 1025 1436 1590 1905
Size: 507 518 411 154 315
Fragments arranged by size:
518 507 411 315 154
NsiI A_TGCA'T
Cuts at: 0 1630 1905
Size: 1630 275
NspI r_CATG'y
Cuts at: 0 1396 1905
Size: 1396 509
PasI CC'CwG_GG
Cuts at: 0 986 1905
Size: 986 919
PciI A'CATG_T
Cuts at: 0 1392 1905
Size: 1392 513
PflMI CCAn_nnn'nTGG
Cuts at: 0 901 1905
Size: 901 1004
PfoI T'CCnGG_A
Cuts at: 0 1327 1905
Size: 1327 578
PpuMI rG'GwC_Cy
Cuts at: 0 239 1905
Size: 239 1666
PsiI TTA'TAA
Cuts at: 0 737 1905
Size: 737 1168
PspOMI G'GGCC_C
Cuts at: 0 352 1905
Size: 352 1553
PstI C_TGCA'G
Cuts at: 0 416 996 1785 1905
Size: 416 580 789 120
Fragments arranged by size:
789 580 416 120
PvuII CAG'CTG
Cuts at: 0 459 1278 1905
Size: 459 819 627
Fragments arranged by size:
819 627 459
SbfI CC_TGCA'GG
Cuts at: 0 416 1785 1905
Size: 416 1369 120
Fragments arranged by size:
1369 416 120
ScaI AGT'ACT
Cuts at: 0 948 1450 1905
Size: 948 502 455
Fragments arranged by size:
948 502 455
SexAI A'CCwGG_T
Cuts at: 0 83 1905
Size: 83 1822
SfcI C'TryA_G
Cuts at: 0 412 992 1781 1905
Size: 412 580 789 124
Fragments arranged by size:
789 580 412 124
SgrAI Cr'CCGG_yG
Cuts at: 0 229 1905
Size: 229 1676
SmaI CCC'GGG
Cuts at: 0 64 1905
Size: 64 1841
SmlI C'TyrA_G
Cuts at: 0 307 870 1905
Size: 307 563 1035
Fragments arranged by size:
1035 563 307
StyI C'CwwG_G
Cuts at: 0 330 499 507 927 1025 1079 1436 1590
Size: 330 169 8 420 98 54 357 154
Cuts at: 1590 1905
Size: 315
Fragments arranged by size:
420 357 330 315 169 154 98 54
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 1112 1905
Size: 133 979 793
Fragments arranged by size:
979 793 133
TatI w'GTAC_w
Cuts at: 0 946 1448 1905
Size: 946 502 457
Fragments arranged by size:
946 502 457
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 898 930 1905
Size: 259 32 607 32 975
Fragments arranged by size:
975 607 259 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 849 1905
Size: 849 1056
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 286 1905
Size: 12 274 1619
Fragments arranged by size:
1619 274 12
XmaI C'CCGG_G
Cuts at: 0 62 1905
Size: 62 1843
XmnI GAAnn'nnTTC
Cuts at: 0 316 1421 1905
Size: 316 1105 484
Fragments arranged by size:
1105 484 316
Enzymes that do cut:
AarI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BbvCI BfrBI BglII Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I MscI
MslI MspA1I NcoI NsiI NspI PasI PciI PflMI
PfoI PpuMI PsiI PspOMI PstI PvuII SbfI ScaI
SexAI SfcI SgrAI SmaI SmlI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BglI BlpI BmtI
BplI BsaI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI
BsmBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoRI EcoRV FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PmeI PmlI PpiI
PshAI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SfiI SfoI SgfI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI ZraI