Restriction map for
pF1KB3332
(Linear) (Six-base) MAPSORT of: KIBB3332.seq Check: 1008 from: 1 to: 3390
KIBB3332.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 399 1391 1939 1950 3255 3390
Size: 399 992 548 11 1305 135
Fragments arranged by size:
1305 992 548 399 135 11
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1330 1962 2036 2507 2606 3104 3390
Size: 781 549 632 74 471 99 498 286
Fragments arranged by size:
781 632 549 498 471 286 99 74
AfeI AGC'GCT
Cuts at: 0 3089 3390
Size: 3089 301
AflII C'TTAA_G
Cuts at: 0 1417 3390
Size: 1417 1973
AflIII A'CryG_T
Cuts at: 0 1559 3390
Size: 1559 1831
AhdI GACnn_n'nnGTC
Cuts at: 0 65 1137 3390
Size: 65 1072 2253
Fragments arranged by size:
2253 1072 65
AlwNI CAG_nnn'CTG
Cuts at: 0 850 2080 3382 3390
Size: 850 1230 1302 8
Fragments arranged by size:
1302 1230 850 8
ApaI G_GGCC'C
Cuts at: 0 26 91 246 831 1927 1986 2008 3143
Size: 26 65 155 585 1096 59 22 1135
Cuts at: 3143 3390
Size: 247
Fragments arranged by size:
1135 1096 585 247 155 65 59 26
22
ApaLI G'TGCA_C
Cuts at: 0 2924 3390
Size: 2924 466
ApoI r'AATT_y
Cuts at: 0 2800 3390
Size: 2800 590
AseI AT'TA_AT
Cuts at: 0 2642 3390
Size: 2642 748
AvaI C'yCGr_G
Cuts at: 0 84 199 620 1898 2374 3007 3390
Size: 84 115 421 1278 476 633 383
Fragments arranged by size:
1278 633 476 421 383 115 84
AvrII C'CTAG_G
Cuts at: 0 963 1692 2124 3390
Size: 963 729 432 1266
Fragments arranged by size:
1266 963 729 432
BamHI G'GATC_C
Cuts at: 0 1184 3390
Size: 1184 2206
BanI G'GyrC_C
Cuts at: 0 2413 3390
Size: 2413 977
BanII G_rGCy'C
Cuts at: 0 26 37 85 91 206 246 358 688
Size: 26 11 48 6 115 40 112 330
Cuts at: 688 831 971 996 1444 1717 1927 1986 2008
Size: 143 140 25 448 273 210 59 22
Cuts at: 2008 2026 3143 3173 3390
Size: 18 1117 30 217
Fragments arranged by size:
1117 448 330 273 217 210 143 140
115 112 59 48 40 30 26 25
22 18 11 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 2729 2888 3390
Size: 2729 159 502
Fragments arranged by size:
2729 502 159
BbvCI CC'TCA_GC
Cuts at: 0 689 1045 1585 2034 2070 3390
Size: 689 356 540 449 36 1320
Fragments arranged by size:
1320 689 540 449 356 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 65 99 1546 1580 3390
Size: 65 34 1447 34 1810
Fragments arranged by size:
1810 1447 65 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2379 3325 3390
Size: 2379 946 65
Fragments arranged by size:
2379 946 65
BglII A'GATC_T
Cuts at: 0 2330 3390
Size: 2330 1060
BlpI GC'TnA_GC
Cuts at: 0 976 1387 3390
Size: 976 411 2003
Fragments arranged by size:
2003 976 411
Bme1580I G_kGCm'C
Cuts at: 0 26 91 246 831 1803 1927 1986 2008
Size: 26 65 155 585 972 124 59 22
Cuts at: 2008 2087 2282 2375 2928 3143 3390
Size: 79 195 93 553 215 247
Fragments arranged by size:
972 585 553 247 215 195 155 124
93 79 65 59 26 22
BmgBI CAC'GTC
Cuts at: 0 292 1560 3390
Size: 292 1268 1830
Fragments arranged by size:
1830 1268 292
BmrI ACTGGGnnnn_n'
Cuts at: 0 1472 2450 2568 3390
Size: 1472 978 118 822
Fragments arranged by size:
1472 978 822 118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1261 1866 3390
Size: 1261 605 1524
Fragments arranged by size:
1524 1261 605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 910 1235 1245 1382 1931 2908 2933
Size: 133 777 325 10 137 549 977 25
Cuts at: 2933 3274 3390
Size: 341 116
Fragments arranged by size:
977 777 549 341 325 137 133 116
25 10
Bpu10I CC'TnA_GC
Cuts at: 0 689 1045 1585 2034 2070 3390
Size: 689 356 540 449 36 1320
Fragments arranged by size:
1320 689 540 449 356 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 1141 1861 3390
Size: 702 439 720 1529
Fragments arranged by size:
1529 720 702 439
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 1851 3390
Size: 73 1778 1539
Fragments arranged by size:
1778 1539 73
BsaBI GATnn'nnATC
Cuts at: 0 1189 3390
Size: 1189 2201
BsaWI w'CCGG_w
Cuts at: 0 162 1529 3390
Size: 162 1367 1861
Fragments arranged by size:
1861 1367 162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 983 1013 1218 1248 1699 1729 1929 1959
Size: 983 30 205 30 451 30 200 30
Cuts at: 1959 2550 2580 2897 2927 2931 2961 3390
Size: 591 30 317 30 4 30 429
Fragments arranged by size:
983 591 451 429 317 205 200 30
30 30 30 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 463 1367 1639 1763 1778 1839 2326 2494
Size: 463 904 272 124 15 61 487 168
Cuts at: 2494 2680 2683 2731 2791 2857 2866 2869 3043
Size: 186 3 48 60 66 9 3 174
Cuts at: 3043 3058 3061 3214 3390
Size: 15 3 153 176
Fragments arranged by size:
904 487 463 272 186 176 174 168
153 124 66 61 60 48 15 15
9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 43 488 552 776 822 2613 3390
Size: 43 445 64 224 46 1791 777
Fragments arranged by size:
1791 777 445 224 64 46 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 796 880 1033 1127 1336 1556 3004
Size: 482 314 84 153 94 209 220 1448
Cuts at: 3004 3247 3390
Size: 243 143
Fragments arranged by size:
1448 482 314 243 220 209 153 143
94 84
BsiEI CG_ry'CG
Cuts at: 0 1197 3390
Size: 1197 2193
BsiHKAI G_wGCw'C
Cuts at: 0 85 996 1717 2928 3390
Size: 85 911 721 1211 462
Fragments arranged by size:
1211 911 721 462 85
BsmI GAATG_Cn'
Cuts at: 0 401 1346 3390
Size: 401 945 2044
Fragments arranged by size:
2044 945 401
BsmBI CGTCTCn'nnnn_
Cuts at: 0 298 1775 3390
Size: 298 1477 1615
Fragments arranged by size:
1615 1477 298
Bsp1286I G_dGCh'C
Cuts at: 0 26 37 85 91 206 246 358 688
Size: 26 11 48 6 115 40 112 330
Cuts at: 688 831 971 996 1444 1717 1803 1927 1986
Size: 143 140 25 448 273 86 124 59
Cuts at: 1986 2008 2026 2087 2282 2375 2928 3143 3173
Size: 22 18 61 195 93 553 215 30
Cuts at: 3173 3390
Size: 217
Fragments arranged by size:
553 448 330 273 217 215 195 143
140 124 115 112 93 86 61 59
48 40 30 26 25 22 18 11
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 399 1142 1308 1391 1939 1950 3255 3390
Size: 399 743 166 83 548 11 1305 135
Fragments arranged by size:
1305 743 548 399 166 135 83 11
BsrBI CCG'CTC
Cuts at: 0 182 3390
Size: 182 3208
BsrDI GCAATG_nn'
Cuts at: 0 1551 3390
Size: 1551 1839
BsrFI r'CCGG_y
Cuts at: 0 1166 3390
Size: 1166 2224
BsrGI T'GTAC_A
Cuts at: 0 1674 3390
Size: 1674 1716
BssSI C'ACGA_G
Cuts at: 0 1710 3390
Size: 1710 1680
BstAPI GCAn_nnn'nTGC
Cuts at: 0 819 850 3390
Size: 819 31 2540
Fragments arranged by size:
2540 819 31
BstEII G'GTnAC_C
Cuts at: 0 1872 3390
Size: 1872 1518
BstXI CCAn_nnnn'nTGG
Cuts at: 0 532 3390
Size: 532 2858
BstYI r'GATC_y
Cuts at: 0 1184 2330 3390
Size: 1184 1146 1060
Fragments arranged by size:
1184 1146 1060
Bsu36I CC'TnA_GG
Cuts at: 0 463 559 1961 2409 3390
Size: 463 96 1402 448 981
Fragments arranged by size:
1402 981 463 448 96
BtgI C'CryG_G
Cuts at: 0 38 91 452 1432 1919 3390
Size: 38 53 361 980 487 1471
Fragments arranged by size:
1471 980 487 361 53 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1955 3390
Size: 1955 1435
BtsI GCAGTG_nn'
Cuts at: 0 83 808 2661 3390
Size: 83 725 1853 729
Fragments arranged by size:
1853 729 725 83
ClaI AT'CG_AT
Cuts at: 0 3338 3390
Size: 3338 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2558 2593 3390
Size: 2558 35 797
Fragments arranged by size:
2558 797 35
DraIII CAC_nnn'GTG
Cuts at: 0 384 3390
Size: 384 3006
EaeI y'GGCC_r
Cuts at: 0 449 1194 1821 3326 3390
Size: 449 745 627 1505 64
Fragments arranged by size:
1505 745 627 449 64
EagI C'GGCC_G
Cuts at: 0 1194 3390
Size: 1194 2196
EarI CTCTTCn'nnn_
Cuts at: 0 2618 2657 2669 2678 2693 2702 2708 2729
Size: 2618 39 12 9 15 9 6 21
Cuts at: 2729 2735 2744 2759 2768 2780 2834 2867 3161
Size: 6 9 15 9 12 54 33 294
Cuts at: 3161 3191 3251 3390
Size: 30 60 139
Fragments arranged by size:
2618 294 139 60 54 39 33 30
21 15 15 12 12 9 9 9
9 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 2522 3390
Size: 88 2434 868
Fragments arranged by size:
2434 868 88
EcoICRI GAG'CTC
Cuts at: 0 83 994 1715 3390
Size: 83 911 721 1675
Fragments arranged by size:
1675 911 721 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 781 910 1235 1245 1330 1382 1931
Size: 133 648 129 325 10 85 52 549
Cuts at: 1931 1962 2036 2507 2606 2908 2933 3104 3274
Size: 31 74 471 99 302 25 171 170
Cuts at: 3274 3390
Size: 116
Fragments arranged by size:
648 549 471 325 302 171 170 133
129 116 99 85 74 52 31 25
10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 835 2054 2623 3134 3390
Size: 835 1219 569 511 256
Fragments arranged by size:
1219 835 569 511 256
EcoO109I rG'GnC_Cy
Cuts at: 0 23 242 321 426 468 827 828 871
Size: 23 219 79 105 42 359 1 43
Cuts at: 871 1212 1328 1368 1689 1924 1965 1982 1983
Size: 341 116 40 321 235 41 17 1
Cuts at: 1983 2005 2041 2059 2077 2208 2509 2608 3139
Size: 22 36 18 18 131 301 99 531
Cuts at: 3139 3140 3390
Size: 1 250
Fragments arranged by size:
531 359 341 321 301 250 235 219
131 116 105 99 79 43 42 41
40 36 23 22 18 18 17 1
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1855 3390
Size: 1855 1535
HaeII r_GCGC'y
Cuts at: 0 1478 1756 3091 3390
Size: 1478 278 1335 299
Fragments arranged by size:
1478 1335 299 278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 51 83 1123 1155 1231 1263 1954 1986
Size: 51 32 1040 32 76 32 691 32
Cuts at: 1986 2302 2334 2550 2582 3390
Size: 316 32 216 32 808
Fragments arranged by size:
1040 808 691 316 216 76 51 32
32 32 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 3173 3390
Size: 720 2453 217
Fragments arranged by size:
2453 720 217
MslI CAynn'nnrTG
Cuts at: 0 517 1079 1600 3390
Size: 517 562 521 1790
Fragments arranged by size:
1790 562 521 517
MspA1I CmG'CkG
Cuts at: 0 40 93 492 959 1408 1434 1721 3316
Size: 40 53 399 467 449 26 287 1595
Cuts at: 3316 3390
Size: 74
Fragments arranged by size:
1595 467 449 399 287 74 53 40
26
NaeI GCC'GGC
Cuts at: 0 1168 3390
Size: 1168 2222
NcoI C'CATG_G
Cuts at: 0 1919 3390
Size: 1919 1471
NgoMIV G'CCGG_C
Cuts at: 0 1166 3390
Size: 1166 2224
PasI CC'CwG_GG
Cuts at: 0 874 2506 3099 3390
Size: 874 1632 593 291
Fragments arranged by size:
1632 874 593 291
PflMI CCAn_nnn'nTGG
Cuts at: 0 702 3390
Size: 702 2688
PfoI T'CCnGG_A
Cuts at: 0 506 1022 1222 3290 3390
Size: 506 516 200 2068 100
Fragments arranged by size:
2068 516 506 200 100
PpuMI rG'GwC_Cy
Cuts at: 0 426 468 1212 1689 2208 2608 3390
Size: 426 42 744 477 519 400 782
Fragments arranged by size:
782 744 519 477 426 400 42
PspOMI G'GGCC_C
Cuts at: 0 22 87 242 827 1923 1982 2004 3139
Size: 22 65 155 585 1096 59 22 1135
Cuts at: 3139 3390
Size: 251
Fragments arranged by size:
1135 1096 585 251 155 65 59 22
22
PstI C_TGCA'G
Cuts at: 0 1916 2502 3252 3390
Size: 1916 586 750 138
Fragments arranged by size:
1916 750 586 138
PvuII CAG'CTG
Cuts at: 0 492 1408 3316 3390
Size: 492 916 1908 74
Fragments arranged by size:
1908 916 492 74
SacI G_AGCT'C
Cuts at: 0 85 996 1717 3390
Size: 85 911 721 1673
Fragments arranged by size:
1673 911 721 85
SacII CC_GC'GG
Cuts at: 0 41 94 1435 3390
Size: 41 53 1341 1955
Fragments arranged by size:
1955 1341 53 41
SapI GCTCTTCn'nnn_
Cuts at: 0 3161 3390
Size: 3161 229
SexAI A'CCwGG_T
Cuts at: 0 1649 3390
Size: 1649 1741
SfcI C'TryA_G
Cuts at: 0 1912 2429 2498 3248 3390
Size: 1912 517 69 750 142
Fragments arranged by size:
1912 750 517 142 69
SmaI CCC'GGG
Cuts at: 0 201 1900 3009 3390
Size: 201 1699 1109 381
Fragments arranged by size:
1699 1109 381 201
SmlI C'TyrA_G
Cuts at: 0 681 1120 1417 1840 3390
Size: 681 439 297 423 1550
Fragments arranged by size:
1550 681 439 423 297
SrfI GCCC'GGGC
Cuts at: 0 201 3390
Size: 201 3189
SspI AAT'ATT
Cuts at: 0 1065 2758 3390
Size: 1065 1693 632
Fragments arranged by size:
1693 1065 632
StuI AGG'CCT
Cuts at: 0 758 1126 1266 1583 2129 2144 3390
Size: 758 368 140 317 546 15 1246
Fragments arranged by size:
1246 758 546 368 317 140 15
StyI C'CwwG_G
Cuts at: 0 753 963 1127 1286 1692 1919 2124 2336
Size: 753 210 164 159 406 227 205 212
Cuts at: 2336 3390
Size: 1054
Fragments arranged by size:
1054 753 406 227 212 210 205 164
159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1302 1393 1660 3390
Size: 1302 91 267 1730
Fragments arranged by size:
1730 1302 267 91
TatI w'GTAC_w
Cuts at: 0 1674 3390
Size: 1674 1716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 983 1015 1929 1961 2583 2615 3390
Size: 983 32 914 32 622 32 775
Fragments arranged by size:
983 914 775 622 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1651 3390
Size: 1651 1739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 444 2016 3390
Size: 444 1572 1374
Fragments arranged by size:
1572 1374 444
XmaI C'CCGG_G
Cuts at: 0 199 1898 3007 3390
Size: 199 1699 1109 383
Fragments arranged by size:
1699 1109 383 199
XmnI GAAnn'nnTTC
Cuts at: 0 2492 3390
Size: 2492 898
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AhdI AlwNI ApaI
ApaLI ApoI AseI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I MmeI MslI MspA1I
NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SacII SapI SexAI SfcI SmaI
SmlI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AleI AlfI AloI
AscI AsiSI BaeI BbeI BciVI BclI BfrBI BmtI
BsaAI BsaHI BsiWI BspEI BspHI BssHII BstBI BstZ17I
DraI DrdI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SwaI XbaI XhoI ZraI