Restriction map for
pF1KB3327
(Linear) (Six-base) MAPSORT of: KIBB3327.seq Check: 5770 from: 1 to: 2685
KIBB3327.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 833 1699 2685
Size: 833 866 986
Fragments arranged by size:
986 866 833
AccI GT'mk_AC
Cuts at: 0 2611 2685
Size: 2611 74
Acc65I G'GTAC_C
Cuts at: 0 37 2685
Size: 37 2648
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 2573 2685
Size: 488 2085 112
Fragments arranged by size:
2085 488 112
AflIII A'CryG_T
Cuts at: 0 834 1096 2685
Size: 834 262 1589
Fragments arranged by size:
1589 834 262
AgeI A'CCGG_T
Cuts at: 0 1258 2685
Size: 1258 1427
AhdI GACnn_n'nnGTC
Cuts at: 0 1541 1678 2685
Size: 1541 137 1007
Fragments arranged by size:
1541 1007 137
AleI CACnn'nnGTG
Cuts at: 0 575 797 2685
Size: 575 222 1888
Fragments arranged by size:
1888 575 222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2548 2685
Size: 2548 137
AlwNI CAG_nnn'CTG
Cuts at: 0 1138 2292 2685
Size: 1138 1154 393
Fragments arranged by size:
1154 1138 393
ApoI r'AATT_y
Cuts at: 0 926 1280 1802 1944 2685
Size: 926 354 522 142 741
Fragments arranged by size:
926 741 522 354 142
AvrII C'CTAG_G
Cuts at: 0 486 2685
Size: 486 2199
BamHI G'GATC_C
Cuts at: 0 399 1869 2685
Size: 399 1470 816
Fragments arranged by size:
1470 816 399
BanI G'GyrC_C
Cuts at: 0 37 751 1383 1734 1957 2532 2685
Size: 37 714 632 351 223 575 153
Fragments arranged by size:
714 632 575 351 223 153 37
BanII G_rGCy'C
Cuts at: 0 269 1954 2685
Size: 269 1685 731
Fragments arranged by size:
1685 731 269
BbsI GAAGACnn'nnnn_
Cuts at: 0 160 1261 1325 1537 2078 2387 2685
Size: 160 1101 64 212 541 309 298
Fragments arranged by size:
1101 541 309 298 212 160 64
BbvCI CC'TCA_GC
Cuts at: 0 1758 2685
Size: 1758 927
BclI T'GATC_A
Cuts at: 0 789 957 2685
Size: 789 168 1728
Fragments arranged by size:
1728 789 168
BglI GCCn_nnn'nGGC
Cuts at: 0 491 2685
Size: 491 2194
BlpI GC'TnA_GC
Cuts at: 0 263 2685
Size: 263 2422
BmtI G_CTAG'C
Cuts at: 0 2435 2685
Size: 2435 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 482 2685
Size: 464 18 2203
Fragments arranged by size:
2203 464 18
Bpu10I CC'TnA_GC
Cuts at: 0 1758 2685
Size: 1758 927
BsaI GGTCTCn'nnnn_
Cuts at: 0 482 1528 1686 2685
Size: 482 1046 158 999
Fragments arranged by size:
1046 999 482 158
BsaHI Gr'CG_yC
Cuts at: 0 316 515 2685
Size: 316 199 2170
Fragments arranged by size:
2170 316 199
BsaWI w'CCGG_w
Cuts at: 0 1258 2685
Size: 1258 1427
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 1666 1696 2124 2154 2685
Size: 400 30 1236 30 428 30 531
Fragments arranged by size:
1236 531 428 400 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 228 421 643 1746 2685
Size: 228 193 222 1103 939
Fragments arranged by size:
1103 939 228 222 193
BseYI C'CCAG_C
Cuts at: 0 566 2685
Size: 566 2119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 984 1151 2685
Size: 891 93 167 1534
Fragments arranged by size:
1534 891 167 93
BsiEI CG_ry'CG
Cuts at: 0 196 209 260 2685
Size: 196 13 51 2425
Fragments arranged by size:
2425 196 51 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2144 2685
Size: 2144 541
Bsp1286I G_dGCh'C
Cuts at: 0 269 1954 2685
Size: 269 1685 731
Fragments arranged by size:
1685 731 269
BspHI T'CATG_A
Cuts at: 0 1788 2685
Size: 1788 897
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 833 1699 2685
Size: 833 866 986
Fragments arranged by size:
986 866 833
BsrDI GCAATG_nn'
Cuts at: 0 229 2191 2685
Size: 229 1962 494
Fragments arranged by size:
1962 494 229
BsrFI r'CCGG_y
Cuts at: 0 40 195 309 1258 2685
Size: 40 155 114 949 1427
Fragments arranged by size:
1427 949 155 114 40
BssSI C'ACGA_G
Cuts at: 0 1892 2685
Size: 1892 793
BstEII G'GTnAC_C
Cuts at: 0 177 2685
Size: 177 2508
BstXI CCAn_nnnn'nTGG
Cuts at: 0 445 2685
Size: 445 2240
BstYI r'GATC_y
Cuts at: 0 399 1869 2224 2442 2685
Size: 399 1470 355 218 243
Fragments arranged by size:
1470 399 355 243 218
BstZ17I GTA'TAC
Cuts at: 0 2612 2685
Size: 2612 73
Bsu36I CC'TnA_GG
Cuts at: 0 404 1911 2685
Size: 404 1507 774
Fragments arranged by size:
1507 774 404
BtgI C'CryG_G
Cuts at: 0 323 395 618 2685
Size: 323 72 223 2067
Fragments arranged by size:
2067 323 223 72
BtsI GCAGTG_nn'
Cuts at: 0 18 2685
Size: 18 2667
DraIII CAC_nnn'GTG
Cuts at: 0 1018 2685
Size: 1018 1667
DrdI GACnn_nn'nnGTC
Cuts at: 0 649 2592 2685
Size: 649 1943 93
Fragments arranged by size:
1943 649 93
EaeI y'GGCC_r
Cuts at: 0 193 206 257 2160 2685
Size: 193 13 51 1903 525
Fragments arranged by size:
1903 525 193 51 13
EagI C'GGCC_G
Cuts at: 0 193 206 257 2685
Size: 193 13 51 2428
Fragments arranged by size:
2428 193 51 13
EarI CTCTTCn'nnn_
Cuts at: 0 464 1372 2685
Size: 464 908 1313
Fragments arranged by size:
1313 908 464
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 47 266 2685
Size: 47 219 2419
Fragments arranged by size:
2419 219 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 482 488 2573 2685
Size: 464 18 6 2085 112
Fragments arranged by size:
2085 464 112 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 339 2017 2685
Size: 339 1678 668
Fragments arranged by size:
1678 668 339
EcoO109I rG'GnC_Cy
Cuts at: 0 344 525 2685
Size: 344 181 2160
Fragments arranged by size:
2160 344 181
EcoRI G'AATT_C
Cuts at: 0 1802 2685
Size: 1802 883
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 734 2685
Size: 734 1951
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 824 856 1527 1559 1664 1696
Size: 400 32 392 32 671 32 105 32
Cuts at: 1696 1825 1857 2295 2317 2327 2349 2403 2430
Size: 129 32 438 22 10 22 54 27
Cuts at: 2430 2435 2462 2685
Size: 5 27 223
Fragments arranged by size:
671 438 400 392 223 129 105 54
32 32 32 32 32 27 27 22
22 10 5
HincII GTy'rAC
Cuts at: 0 2276 2685
Size: 2276 409
KpnI G_GTAC'C
Cuts at: 0 41 2685
Size: 41 2644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 717 1868 2040 2685
Size: 717 1151 172 645
Fragments arranged by size:
1151 717 645 172
MslI CAynn'nnrTG
Cuts at: 0 443 545 575 785 797 951 1035 2685
Size: 443 102 30 210 12 154 84 1650
Fragments arranged by size:
1650 443 210 154 102 84 30 12
MspA1I CmG'CkG
Cuts at: 0 107 262 325 397 2685
Size: 107 155 63 72 2288
Fragments arranged by size:
2288 155 107 72 63
NaeI GCC'GGC
Cuts at: 0 197 311 2685
Size: 197 114 2374
Fragments arranged by size:
2374 197 114
NcoI C'CATG_G
Cuts at: 0 618 2685
Size: 618 2067
NdeI CA'TA_TG
Cuts at: 0 992 2685
Size: 992 1693
NgoMIV G'CCGG_C
Cuts at: 0 195 309 2685
Size: 195 114 2376
Fragments arranged by size:
2376 195 114
NheI G'CTAG_C
Cuts at: 0 2431 2685
Size: 2431 254
NspI r_CATG'y
Cuts at: 0 550 838 1100 1302 2685
Size: 550 288 262 202 1383
Fragments arranged by size:
1383 550 288 262 202
PasI CC'CwG_GG
Cuts at: 0 141 2685
Size: 141 2544
PciI A'CATG_T
Cuts at: 0 834 1096 2685
Size: 834 262 1589
Fragments arranged by size:
1589 834 262
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 2292 2685
Size: 147 2145 393
Fragments arranged by size:
2145 393 147
PfoI T'CCnGG_A
Cuts at: 0 521 2685
Size: 521 2164
PpuMI rG'GwC_Cy
Cuts at: 0 344 525 2685
Size: 344 181 2160
Fragments arranged by size:
2160 344 181
PstI C_TGCA'G
Cuts at: 0 1376 1710 2685
Size: 1376 334 975
Fragments arranged by size:
1376 975 334
SacII CC_GC'GG
Cuts at: 0 326 398 2685
Size: 326 72 2287
Fragments arranged by size:
2287 326 72
SfcI C'TryA_G
Cuts at: 0 795 910 1372 1706 2685
Size: 795 115 462 334 979
Fragments arranged by size:
979 795 462 334 115
SpeI A'CTAG_T
Cuts at: 0 1126 2685
Size: 1126 1559
SspI AAT'ATT
Cuts at: 0 1881 2685
Size: 1881 804
StuI AGG'CCT
Cuts at: 0 485 2685
Size: 485 2200
StyI C'CwwG_G
Cuts at: 0 486 618 2685
Size: 486 132 2067
Fragments arranged by size:
2067 486 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1975 2679 2685
Size: 1975 704 6
Fragments arranged by size:
1975 704 6
TatI w'GTAC_w
Cuts at: 0 1555 2647 2685
Size: 1555 1092 38
Fragments arranged by size:
1555 1092 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 696 728 1002 1034 1666 1698 2097 2124
Size: 696 32 274 32 632 32 399 27
Cuts at: 2124 2129 2156 2685
Size: 5 27 529
Fragments arranged by size:
696 632 529 399 274 32 32 32
27 27 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 442 729 1558 2685
Size: 442 287 829 1127
Fragments arranged by size:
1127 829 442 287
XmnI GAAnn'nnTTC
Cuts at: 0 1931 2685
Size: 1931 754
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApoI AvrII BamHI BanI BanII BbsI
BbvCI BclI BglI BlpI BmtI BpmI Bpu10I BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HincII KpnI MmeI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NspI PasI PciI PflMI PfoI
PpuMI PstI SacII SfcI SpeI SspI StuI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AloI ApaI ApaLI AscI
AseI AsiSI AvaI BaeI BbeI BcgI BciVI BfrBI
BglII Bme1580I BmgBI BmrI BplI BpuEI BsaAI BsaBI
BsiHKAI BsiWI BsmI BspEI BsrBI BsrGI BssHII BstAPI
BstBI BtgZI ClaI CspCI DraI EcoICRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
MscI NarI NotI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI
SrfI SwaI Tth111I XbaI XhoI XmaI ZraI