Restriction map for
pF1KB3321
(Linear) (Six-base) MAPSORT of: KIBB3321.seq Check: 4765 from: 1 to: 2769
KIBB3321.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 24 340 1785 1810 2769
Size: 24 316 1445 25 959
Fragments arranged by size:
1445 959 316 25 24
AccI GT'mk_AC
Cuts at: 0 1156 2396 2769
Size: 1156 1240 373
Fragments arranged by size:
1240 1156 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2734 2769
Size: 2734 35
AfeI AGC'GCT
Cuts at: 0 2116 2769
Size: 2116 653
AgeI A'CCGG_T
Cuts at: 0 545 1151 2769
Size: 545 606 1618
Fragments arranged by size:
1618 606 545
AhdI GACnn_n'nnGTC
Cuts at: 0 433 2769
Size: 433 2336
AleI CACnn'nnGTG
Cuts at: 0 2494 2769
Size: 2494 275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1102 1862 2769
Size: 1102 760 907
Fragments arranged by size:
1102 907 760
AlwNI CAG_nnn'CTG
Cuts at: 0 174 210 474 1652 1800 2085 2667 2693
Size: 174 36 264 1178 148 285 582 26
Cuts at: 2693 2712 2769
Size: 19 57
Fragments arranged by size:
1178 582 285 264 174 148 57 36
26 19
ApaI G_GGCC'C
Cuts at: 0 560 1949 2077 2196 2769
Size: 560 1389 128 119 573
Fragments arranged by size:
1389 573 560 128 119
AvaI C'yCGr_G
Cuts at: 0 553 559 565 959 2189 2359 2753 2769
Size: 553 6 6 394 1230 170 394 16
Fragments arranged by size:
1230 553 394 394 170 16 6 6
BamHI G'GATC_C
Cuts at: 0 2373 2769
Size: 2373 396
BanI G'GyrC_C
Cuts at: 0 102 487 1114 1171 2769
Size: 102 385 627 57 1598
Fragments arranged by size:
1598 627 385 102 57
BanII G_rGCy'C
Cuts at: 0 90 560 566 572 1637 1877 1949 1960
Size: 90 470 6 6 1065 240 72 11
Cuts at: 1960 2077 2196 2769
Size: 117 119 573
Fragments arranged by size:
1065 573 470 240 119 117 90 72
11 6 6
BbeI G_GCGC'C
Cuts at: 0 1118 2769
Size: 1118 1651
BbsI GAAGACnn'nnnn_
Cuts at: 0 992 1272 2769
Size: 992 280 1497
Fragments arranged by size:
1497 992 280
BbvCI CC'TCA_GC
Cuts at: 0 1252 1519 1964 2281 2769
Size: 1252 267 445 317 488
Fragments arranged by size:
1252 488 445 317 267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 329 2769
Size: 295 34 2440
Fragments arranged by size:
2440 295 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2050 2769
Size: 2050 719
BfrBI ATG'CAT
Cuts at: 0 1579 2769
Size: 1579 1190
BglI GCCn_nnn'nGGC
Cuts at: 0 1539 1614 2769
Size: 1539 75 1155
Fragments arranged by size:
1539 1155 75
BglII A'GATC_T
Cuts at: 0 1391 2769
Size: 1391 1378
Bme1580I G_kGCm'C
Cuts at: 0 492 560 841 1949 2077 2183 2196 2769
Size: 492 68 281 1108 128 106 13 573
Fragments arranged by size:
1108 573 492 281 128 106 68 13
BmgBI CAC'GTC
Cuts at: 0 99 2769
Size: 99 2670
BmrI ACTGGGnnnn_n'
Cuts at: 0 522 2157 2769
Size: 522 1635 612
Fragments arranged by size:
1635 612 522
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 291 1122 1124 2769
Size: 291 831 2 1645
Fragments arranged by size:
1645 831 291 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 286 1117 1453 2043 2131 2769
Size: 199 87 831 336 590 88 638
Fragments arranged by size:
831 638 590 336 199 88 87
Bpu10I CC'TnA_GC
Cuts at: 0 292 1252 1519 1964 2203 2281 2668 2769
Size: 292 960 267 445 239 78 387 101
Fragments arranged by size:
960 445 387 292 267 239 101 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2218 2769
Size: 2218 551
BsaI GGTCTCn'nnnn_
Cuts at: 0 344 2769
Size: 344 2425
BsaHI Gr'CG_yC
Cuts at: 0 641 1115 2769
Size: 641 474 1654
Fragments arranged by size:
1654 641 474
BsaWI w'CCGG_w
Cuts at: 0 353 545 1151 2339 2769
Size: 353 192 606 1188 430
Fragments arranged by size:
1188 606 430 353 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 583 613 2769
Size: 583 30 2156
Fragments arranged by size:
2156 583 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 607 610 727 1574 2769
Size: 253 354 3 117 847 1195
Fragments arranged by size:
1195 847 354 253 117 3
BseYI C'CCAG_C
Cuts at: 0 60 298 626 1288 1941 2070 2078 2195
Size: 60 238 328 662 653 129 8 117
Cuts at: 2195 2769
Size: 574
Fragments arranged by size:
662 653 574 328 238 129 117 60
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1300 2539 2769
Size: 706 594 1239 230
Fragments arranged by size:
1239 706 594 230
BsiHKAI G_wGCw'C
Cuts at: 0 75 185 293 1230 2674 2769
Size: 75 110 108 937 1444 95
Fragments arranged by size:
1444 937 110 108 95 75
BsmI GAATG_Cn'
Cuts at: 0 1581 2769
Size: 1581 1188
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1891 2769
Size: 1891 878
Bsp1286I G_dGCh'C
Cuts at: 0 75 90 185 293 492 560 566 572
Size: 75 15 95 108 199 68 6 6
Cuts at: 572 841 1230 1637 1877 1949 1960 2077 2183
Size: 269 389 407 240 72 11 117 106
Cuts at: 2183 2196 2674 2769
Size: 13 478 95
Fragments arranged by size:
478 407 389 269 240 199 117 108
106 95 95 75 72 68 15 13
11 6 6
BspEI T'CCGG_A
Cuts at: 0 2339 2769
Size: 2339 430
BspHI T'CATG_A
Cuts at: 0 582 1230 2475 2769
Size: 582 648 1245 294
Fragments arranged by size:
1245 648 582 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 340 1523 1785 1810 2769
Size: 24 316 1183 262 25 959
Fragments arranged by size:
1183 959 316 262 25 24
BsrBI CCG'CTC
Cuts at: 0 285 2322 2769
Size: 285 2037 447
Fragments arranged by size:
2037 447 285
BsrFI r'CCGG_y
Cuts at: 0 146 545 1117 1151 1694 2769
Size: 146 399 572 34 543 1075
Fragments arranged by size:
1075 572 543 399 146 34
BssSI C'ACGA_G
Cuts at: 0 2620 2769
Size: 2620 149
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1395 1825 2769
Size: 1395 430 944
Fragments arranged by size:
1395 944 430
BstBI TT'CG_AA
Cuts at: 0 663 2769
Size: 663 2106
BstEII G'GTnAC_C
Cuts at: 0 879 900 2769
Size: 879 21 1869
Fragments arranged by size:
1869 879 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1065 1777 1869 1995 2769
Size: 1065 712 92 126 774
Fragments arranged by size:
1065 774 712 126 92
BstYI r'GATC_y
Cuts at: 0 413 865 1184 1391 2373 2769
Size: 413 452 319 207 982 396
Fragments arranged by size:
982 452 413 396 319 207
BstZ17I GTA'TAC
Cuts at: 0 1157 2397 2769
Size: 1157 1240 372
Fragments arranged by size:
1240 1157 372
Bsu36I CC'TnA_GG
Cuts at: 0 367 2769
Size: 367 2402
BtgI C'CryG_G
Cuts at: 0 76 572 885 1208 1851 2769
Size: 76 496 313 323 643 918
Fragments arranged by size:
918 643 496 323 313 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1560 2769
Size: 1560 1209
BtsI GCAGTG_nn'
Cuts at: 0 388 1534 2559 2769
Size: 388 1146 1025 210
Fragments arranged by size:
1146 1025 388 210
DraIII CAC_nnn'GTG
Cuts at: 0 845 1710 2049 2067 2769
Size: 845 865 339 18 702
Fragments arranged by size:
865 845 702 339 18
EaeI y'GGCC_r
Cuts at: 0 13 326 393 602 1454 1531 1696 1859
Size: 13 313 67 209 852 77 165 163
Cuts at: 1859 2153 2291 2534 2769
Size: 294 138 243 235
Fragments arranged by size:
852 313 294 243 235 209 165 163
138 77 67 13
EarI CTCTTCn'nnn_
Cuts at: 0 686 2035 2769
Size: 686 1349 734
Fragments arranged by size:
1349 734 686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2343 2769
Size: 2343 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 286 1117 1453 2043 2131 2734 2769
Size: 199 87 831 336 590 88 603 35
Fragments arranged by size:
831 603 590 336 199 88 87 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 268 913 2620 2769
Size: 268 645 1707 149
Fragments arranged by size:
1707 645 268 149
EcoO109I rG'GnC_Cy
Cuts at: 0 634 1945 1992 2074 2769
Size: 634 1311 47 82 695
Fragments arranged by size:
1311 695 634 82 47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1038 2769
Size: 1038 1731
HaeII r_GCGC'y
Cuts at: 0 1118 1295 2118 2769
Size: 1118 177 823 651
Fragments arranged by size:
1118 823 651 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 419 451 2393 2425 2769
Size: 419 32 1942 32 344
Fragments arranged by size:
1942 419 344 32 32
HincII GTy'rAC
Cuts at: 0 873 1715 1899 2769
Size: 873 842 184 870
Fragments arranged by size:
873 870 842 184
KasI G'GCGC_C
Cuts at: 0 1114 2769
Size: 1114 1655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1181 1538 2769
Size: 773 408 357 1231
Fragments arranged by size:
1231 773 408 357
MscI TGG'CCA
Cuts at: 0 15 328 1456 1533 1861 2155 2536 2769
Size: 15 313 1128 77 328 294 381 233
Fragments arranged by size:
1128 381 328 313 294 233 77 15
MslI CAynn'nnrTG
Cuts at: 0 1325 2494 2769
Size: 1325 1169 275
Fragments arranged by size:
1325 1169 275
MspA1I CmG'CkG
Cuts at: 0 78 171 309 630 936 1652 1725 1941
Size: 78 93 138 321 306 716 73 216
Cuts at: 1941 2007 2199 2712 2733 2769
Size: 66 192 513 21 36
Fragments arranged by size:
716 513 321 306 216 192 138 93
78 73 66 36 21
NaeI GCC'GGC
Cuts at: 0 1119 1696 2769
Size: 1119 577 1073
Fragments arranged by size:
1119 1073 577
NarI GG'CG_CC
Cuts at: 0 1115 2769
Size: 1115 1654
NcoI C'CATG_G
Cuts at: 0 885 1208 2769
Size: 885 323 1561
Fragments arranged by size:
1561 885 323
NgoMIV G'CCGG_C
Cuts at: 0 1117 1694 2769
Size: 1117 577 1075
Fragments arranged by size:
1117 1075 577
NsiI A_TGCA'T
Cuts at: 0 1581 2769
Size: 1581 1188
NspI r_CATG'y
Cuts at: 0 2654 2769
Size: 2654 115
PasI CC'CwG_GG
Cuts at: 0 423 2769
Size: 423 2346
PflMI CCAn_nnn'nTGG
Cuts at: 0 456 1416 1557 2049 2693 2769
Size: 456 960 141 492 644 76
Fragments arranged by size:
960 644 492 456 141 76
PfoI T'CCnGG_A
Cuts at: 0 442 521 2769
Size: 442 79 2248
Fragments arranged by size:
2248 442 79
PpuMI rG'GwC_Cy
Cuts at: 0 1992 2769
Size: 1992 777
PshAI GACnn'nnGTC
Cuts at: 0 992 2769
Size: 992 1777
PspOMI G'GGCC_C
Cuts at: 0 556 1945 2073 2192 2769
Size: 556 1389 128 119 577
Fragments arranged by size:
1389 577 556 128 119
PstI C_TGCA'G
Cuts at: 0 1724 1821 1841 2144 2769
Size: 1724 97 20 303 625
Fragments arranged by size:
1724 625 303 97 20
PvuII CAG'CTG
Cuts at: 0 171 309 936 1652 1725 1941 2007 2199
Size: 171 138 627 716 73 216 66 192
Cuts at: 2199 2712 2769
Size: 513 57
Fragments arranged by size:
716 627 513 216 192 171 138 73
66 57
SacII CC_GC'GG
Cuts at: 0 79 2769
Size: 79 2690
SapI GCTCTTCn'nnn_
Cuts at: 0 686 2769
Size: 686 2083
ScaI AGT'ACT
Cuts at: 0 2146 2769
Size: 2146 623
SexAI A'CCwGG_T
Cuts at: 0 1484 1976 2045 2769
Size: 1484 492 69 724
Fragments arranged by size:
1484 724 492 69
SfcI C'TryA_G
Cuts at: 0 1720 1817 1837 2140 2769
Size: 1720 97 20 303 629
Fragments arranged by size:
1720 629 303 97 20
SfoI GGC'GCC
Cuts at: 0 1116 2769
Size: 1116 1653
SmaI CCC'GGG
Cuts at: 0 555 961 2191 2769
Size: 555 406 1230 578
Fragments arranged by size:
1230 578 555 406
SmlI C'TyrA_G
Cuts at: 0 2233 2359 2769
Size: 2233 126 410
Fragments arranged by size:
2233 410 126
SpeI A'CTAG_T
Cuts at: 0 2493 2769
Size: 2493 276
StuI AGG'CCT
Cuts at: 0 274 2769
Size: 274 2495
StyI C'CwwG_G
Cuts at: 0 885 1208 1787 1988 2769
Size: 885 323 579 201 781
Fragments arranged by size:
885 781 579 323 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 542 1404 2139 2769
Size: 542 862 735 630
Fragments arranged by size:
862 735 630 542
TatI w'GTAC_w
Cuts at: 0 2144 2348 2769
Size: 2144 204 421
Fragments arranged by size:
2144 421 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2664 2696 2769
Size: 2664 32 73
Fragments arranged by size:
2664 73 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2106 2690 2769
Size: 2106 584 79
Fragments arranged by size:
2106 584 79
XhoI C'TCGA_G
Cuts at: 0 2359 2769
Size: 2359 410
XmaI C'CCGG_G
Cuts at: 0 553 959 2189 2769
Size: 553 406 1230 580
Fragments arranged by size:
1230 580 553 406
Enzymes that do cut:
AarI AccI AcuI AfeI AgeI AhdI AleI AlfI
AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII SacII SapI
ScaI SexAI SfcI SfoI SmaI SmlI SpeI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BclI BlpI BmtI
BsaAI BsaBI BsiEI BsiWI BsrDI BsrGI BssHII ClaI
CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SbfI SfiI SgfI SgrAI SnaBI SphI SrfI SspI
SwaI Tth111I XbaI XmnI ZraI