Restriction map for
pF1KB3277
(Linear) (Six-base) MAPSORT of: KIBB3277.seq Check: 5355 from: 1 to: 1815
KIBB3277.orf
With 182 enzymes: SgfI *
April 12, 2016 09:51 ..
Acc65I G'GTAC_C
Cuts at: 0 666 1815
Size: 666 1149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1403 1815
Size: 727 676 412
Fragments arranged by size:
727 676 412
AfeI AGC'GCT
Cuts at: 0 743 1815
Size: 743 1072
AhdI GACnn_n'nnGTC
Cuts at: 0 685 1815
Size: 685 1130
AlwNI CAG_nnn'CTG
Cuts at: 0 241 1815
Size: 241 1574
ApaI G_GGCC'C
Cuts at: 0 700 1815
Size: 700 1115
ApoI r'AATT_y
Cuts at: 0 292 415 441 1815
Size: 292 123 26 1374
Fragments arranged by size:
1374 292 123 26
AseI AT'TA_AT
Cuts at: 0 968 1815
Size: 968 847
AvaI C'yCGr_G
Cuts at: 0 1064 1661 1815
Size: 1064 597 154
Fragments arranged by size:
1064 597 154
BanI G'GyrC_C
Cuts at: 0 666 1652 1815
Size: 666 986 163
Fragments arranged by size:
986 666 163
BanII G_rGCy'C
Cuts at: 0 700 1815
Size: 700 1115
BbsI GAAGACnn'nnnn_
Cuts at: 0 643 1815
Size: 643 1172
BbvCI CC'TCA_GC
Cuts at: 0 1437 1815
Size: 1437 378
BciVI GTATCCnnnnn_n'
Cuts at: 0 954 1815
Size: 954 861
BclI T'GATC_A
Cuts at: 0 1335 1815
Size: 1335 480
BlpI GC'TnA_GC
Cuts at: 0 885 1815
Size: 885 930
Bme1580I G_kGCm'C
Cuts at: 0 700 1815
Size: 700 1115
BmgBI CAC'GTC
Cuts at: 0 1668 1815
Size: 1668 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 355 572 836 1815
Size: 355 217 264 979
Fragments arranged by size:
979 355 264 217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 408 1118 1225 1467 1815
Size: 118 290 710 107 242 348
Fragments arranged by size:
710 348 290 242 118 107
Bpu10I CC'TnA_GC
Cuts at: 0 1261 1437 1815
Size: 1261 176 378
Fragments arranged by size:
1261 378 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1061 1192 1313 1815
Size: 1061 131 121 502
Fragments arranged by size:
1061 502 131 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 1606 1815
Size: 1606 209
BsaAI yAC'GTr
Cuts at: 0 1536 1569 1815
Size: 1536 33 246
Fragments arranged by size:
1536 246 33
BsaBI GATnn'nnATC
Cuts at: 0 784 1815
Size: 784 1031
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 1215 1245 1815
Size: 550 30 635 30 570
Fragments arranged by size:
635 570 550 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 641 1425 1815
Size: 115 526 784 390
Fragments arranged by size:
784 526 390 115
BseYI C'CCAG_C
Cuts at: 0 1234 1418 1780 1815
Size: 1234 184 362 35
Fragments arranged by size:
1234 362 184 35
BsiHKAI G_wGCw'C
Cuts at: 0 647 1190 1815
Size: 647 543 625
Fragments arranged by size:
647 625 543
BsmI GAATG_Cn'
Cuts at: 0 626 1815
Size: 626 1189
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1601 1815
Size: 1601 214
Bsp1286I G_dGCh'C
Cuts at: 0 647 700 1190 1815
Size: 647 53 490 625
Fragments arranged by size:
647 625 490 53
BsrBI CCG'CTC
Cuts at: 0 135 566 1357 1427 1815
Size: 135 431 791 70 388
Fragments arranged by size:
791 431 388 135 70
BsrDI GCAATG_nn'
Cuts at: 0 934 1815
Size: 934 881
BsrFI r'CCGG_y
Cuts at: 0 26 1815
Size: 26 1789
BstBI TT'CG_AA
Cuts at: 0 260 1815
Size: 260 1555
BstEII G'GTnAC_C
Cuts at: 0 795 1815
Size: 795 1020
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1462 1815
Size: 1462 353
BstYI r'GATC_y
Cuts at: 0 865 1815
Size: 865 950
Bsu36I CC'TnA_GG
Cuts at: 0 1037 1815
Size: 1037 778
BtgI C'CryG_G
Cuts at: 0 1617 1815
Size: 1617 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 7 1441 1815
Size: 7 1434 374
Fragments arranged by size:
1434 374 7
BtsI GCAGTG_nn'
Cuts at: 0 10 122 1467 1632 1815
Size: 10 112 1345 165 183
Fragments arranged by size:
1345 183 165 112 10
DraI TTT'AAA
Cuts at: 0 758 1815
Size: 758 1057
DraIII CAC_nnn'GTG
Cuts at: 0 1591 1815
Size: 1591 224
EaeI y'GGCC_r
Cuts at: 0 248 1815
Size: 248 1567
EarI CTCTTCn'nnn_
Cuts at: 0 1434 1815
Size: 1434 381
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 792 1798 1815
Size: 792 1006 17
Fragments arranged by size:
1006 792 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 408 727 1118 1225 1403 1467 1815
Size: 118 290 319 391 107 178 64 348
Fragments arranged by size:
391 348 319 290 178 118 107 64
EcoO109I rG'GnC_Cy
Cuts at: 0 697 1815
Size: 697 1118
EcoRV GAT'ATC
Cuts at: 0 782 1760 1815
Size: 782 978 55
Fragments arranged by size:
978 782 55
FspI TGC'GCA
Cuts at: 0 121 1815
Size: 121 1694
HaeII r_GCGC'y
Cuts at: 0 745 1815
Size: 745 1070
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 671 703 1017 1049 1332 1364 1815
Size: 671 32 314 32 283 32 451
Fragments arranged by size:
671 451 314 283 32 32 32
HindIII A'AGCT_T
Cuts at: 0 882 1815
Size: 882 933
KpnI G_GTAC'C
Cuts at: 0 670 1815
Size: 670 1145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 893 1815
Size: 134 759 922
Fragments arranged by size:
922 759 134
MscI TGG'CCA
Cuts at: 0 250 1815
Size: 250 1565
MslI CAynn'nnrTG
Cuts at: 0 224 727 1460 1815
Size: 224 503 733 355
Fragments arranged by size:
733 503 355 224
MspA1I CmG'CkG
Cuts at: 0 802 1111 1815
Size: 802 309 704
Fragments arranged by size:
802 704 309
NaeI GCC'GGC
Cuts at: 0 28 1815
Size: 28 1787
NgoMIV G'CCGG_C
Cuts at: 0 26 1815
Size: 26 1789
NspI r_CATG'y
Cuts at: 0 283 1815
Size: 283 1532
PflMI CCAn_nnn'nTGG
Cuts at: 0 241 1815
Size: 241 1574
PmlI CAC'GTG
Cuts at: 0 1569 1815
Size: 1569 246
PshAI GACnn'nnGTC
Cuts at: 0 199 1815
Size: 199 1616
PspOMI G'GGCC_C
Cuts at: 0 696 1815
Size: 696 1119
PstI C_TGCA'G
Cuts at: 0 389 1815
Size: 389 1426
PvuII CAG'CTG
Cuts at: 0 1111 1815
Size: 1111 704
SapI GCTCTTCn'nnn_
Cuts at: 0 1434 1815
Size: 1434 381
SfcI C'TryA_G
Cuts at: 0 207 385 506 1815
Size: 207 178 121 1309
Fragments arranged by size:
1309 207 178 121
SgrAI Cr'CCGG_yG
Cuts at: 0 26 1815
Size: 26 1789
SmaI CCC'GGG
Cuts at: 0 1663 1815
Size: 1663 152
SmlI C'TyrA_G
Cuts at: 0 1064 1076 1171 1328 1815
Size: 1064 12 95 157 487
Fragments arranged by size:
1064 487 157 95 12
SnaBI TAC'GTA
Cuts at: 0 1536 1815
Size: 1536 279
StuI AGG'CCT
Cuts at: 0 1210 1815
Size: 1210 605
StyI C'CwwG_G
Cuts at: 0 998 1724 1815
Size: 998 726 91
Fragments arranged by size:
998 726 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 448 1240 1815
Size: 448 792 575
Fragments arranged by size:
792 575 448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 89 1215 1247 1414 1446 1630 1662
Size: 57 32 1126 32 167 32 184 32
Cuts at: 1662 1815
Size: 153
Fragments arranged by size:
1126 184 167 153 57 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 681 1815
Size: 681 1134
XhoI C'TCGA_G
Cuts at: 0 1064 1815
Size: 1064 751
XmaI C'CCGG_G
Cuts at: 0 1661 1815
Size: 1661 154
Enzymes that do cut:
Acc65I AcuI AfeI AhdI AlwNI ApaI ApoI AseI
AvaI BanI BanII BbsI BbvCI BciVI BclI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BsrBI BsrDI BsrFI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII EaeI EarI EciI
Eco57MI EcoO109I EcoRV FspI HaeII Hin4I HindIII KpnI
MmeI MscI MslI MspA1I NaeI NgoMIV NspI PflMI
PmlI PshAI PspOMI PstI PvuII SapI SfcI SgrAI
SmaI SmlI SnaBI StuI StyI TaqII TstI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AleI
AlfI AloI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BcgI BfrBI BglI BglII BmtI BplI BsaHI
BsaWI BsgI BsiEI BsiWI BspEI BspHI BspMI BsrGI
BssHII BssSI BstAPI BstZ17I ClaI CspCI DrdI EagI
EcoICRI EcoNI EcoRI FalI FseI FspAI HincII HpaI
KasI MfeI MluI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PpiI
PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XmnI ZraI