Restriction map for
pF1KB3214
(Linear) (Six-base) MAPSORT of: KIBB3214.seq Check: 2804 from: 1 to: 2523
KIBB3214.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
Acc65I G'GTAC_C
Cuts at: 0 1503 2523
Size: 1503 1020
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 2523
Size: 360 2163
AfeI AGC'GCT
Cuts at: 0 1308 1984 2523
Size: 1308 676 539
Fragments arranged by size:
1308 676 539
AflII C'TTAA_G
Cuts at: 0 1020 2523
Size: 1020 1503
AflIII A'CryG_T
Cuts at: 0 668 2523
Size: 668 1855
AhdI GACnn_n'nnGTC
Cuts at: 0 1803 2523
Size: 1803 720
AleI CACnn'nnGTG
Cuts at: 0 1330 2523
Size: 1330 1193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1106 1348 2279 2523
Size: 1106 242 931 244
Fragments arranged by size:
1106 931 244 242
AlwNI CAG_nnn'CTG
Cuts at: 0 195 399 1565 2175 2523
Size: 195 204 1166 610 348
Fragments arranged by size:
1166 610 348 204 195
ApaI G_GGCC'C
Cuts at: 0 150 2523
Size: 150 2373
ApoI r'AATT_y
Cuts at: 0 2016 2523
Size: 2016 507
AvaI C'yCGr_G
Cuts at: 0 622 2158 2400 2523
Size: 622 1536 242 123
Fragments arranged by size:
1536 622 242 123
BanI G'GyrC_C
Cuts at: 0 1383 1503 2343 2523
Size: 1383 120 840 180
Fragments arranged by size:
1383 840 180 120
BanII G_rGCy'C
Cuts at: 0 150 566 658 1606 2158 2523
Size: 150 416 92 948 552 365
Fragments arranged by size:
948 552 416 365 150 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 457 491 2523
Size: 457 34 2032
Fragments arranged by size:
2032 457 34
BfrBI ATG'CAT
Cuts at: 0 733 2093 2523
Size: 733 1360 430
Fragments arranged by size:
1360 733 430
BglI GCCn_nnn'nGGC
Cuts at: 0 154 532 2523
Size: 154 378 1991
Fragments arranged by size:
1991 378 154
BglII A'GATC_T
Cuts at: 0 1869 2523
Size: 1869 654
BlpI GC'TnA_GC
Cuts at: 0 2252 2523
Size: 2252 271
Bme1580I G_kGCm'C
Cuts at: 0 150 638 2300 2346 2523
Size: 150 488 1662 46 177
Fragments arranged by size:
1662 488 177 150 46
BmgBI CAC'GTC
Cuts at: 0 114 633 2523
Size: 114 519 1890
Fragments arranged by size:
1890 519 114
BmrI ACTGGGnnnn_n'
Cuts at: 0 833 1064 2523
Size: 833 231 1459
Fragments arranged by size:
1459 833 231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1653 2441 2523
Size: 1653 788 82
Fragments arranged by size:
1653 788 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 902 1006 1287 1749 2208 2523
Size: 382 520 104 281 462 459 315
Fragments arranged by size:
520 462 459 382 315 281 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 2523
Size: 781 1742
BsaI GGTCTCn'nnnn_
Cuts at: 0 1031 1534 2523
Size: 1031 503 989
Fragments arranged by size:
1031 989 503
BsaAI yAC'GTr
Cuts at: 0 1188 2523
Size: 1188 1335
BsaHI Gr'CG_yC
Cuts at: 0 896 2523
Size: 896 1627
BsaWI w'CCGG_w
Cuts at: 0 929 1280 1777 2523
Size: 929 351 497 746
Fragments arranged by size:
929 746 497 351
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2101 2131 2523
Size: 2101 30 392
Fragments arranged by size:
2101 392 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 499 502 514 517 1290 1299 1626
Size: 253 246 3 12 3 773 9 327
Cuts at: 1626 1629 2394 2417 2523
Size: 3 765 23 106
Fragments arranged by size:
773 765 327 253 246 106 23 12
9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 392 940 966 970 1423 1979 2347 2523
Size: 392 548 26 4 453 556 368 176
Fragments arranged by size:
556 548 453 392 368 176 26 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 1837 2523
Size: 42 1795 686
Fragments arranged by size:
1795 686 42
BsiHKAI G_wGCw'C
Cuts at: 0 658 1327 2523
Size: 658 669 1196
Fragments arranged by size:
1196 669 658
BsmI GAATG_Cn'
Cuts at: 0 1335 2523
Size: 1335 1188
BsmBI CGTCTCn'nnnn_
Cuts at: 0 499 514 2523
Size: 499 15 2009
Fragments arranged by size:
2009 499 15
Bsp1286I G_dGCh'C
Cuts at: 0 150 566 638 658 1327 1606 2158 2300
Size: 150 416 72 20 669 279 552 142
Cuts at: 2300 2346 2523
Size: 46 177
Fragments arranged by size:
669 552 416 279 177 150 142 72
46 20
BspEI T'CCGG_A
Cuts at: 0 1777 2523
Size: 1777 746
BspHI T'CATG_A
Cuts at: 0 1941 2523
Size: 1941 582
BsrBI CCG'CTC
Cuts at: 0 910 1598 2523
Size: 910 688 925
Fragments arranged by size:
925 910 688
BsrDI GCAATG_nn'
Cuts at: 0 2261 2523
Size: 2261 262
BsrFI r'CCGG_y
Cuts at: 0 1380 1408 2523
Size: 1380 28 1115
Fragments arranged by size:
1380 1115 28
BsrGI T'GTAC_A
Cuts at: 0 1712 2488 2523
Size: 1712 776 35
Fragments arranged by size:
1712 776 35
BssSI C'ACGA_G
Cuts at: 0 457 2523
Size: 457 2066
BstEII G'GTnAC_C
Cuts at: 0 1135 2523
Size: 1135 1388
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1002 2523
Size: 1002 1521
BstYI r'GATC_y
Cuts at: 0 991 1869 2165 2523
Size: 991 878 296 358
Fragments arranged by size:
991 878 358 296
Bsu36I CC'TnA_GG
Cuts at: 0 236 750 1231 2523
Size: 236 514 481 1292
Fragments arranged by size:
1292 514 481 236
BtgI C'CryG_G
Cuts at: 0 959 1217 1443 2204 2216 2456 2523
Size: 959 258 226 761 12 240 67
Fragments arranged by size:
959 761 258 240 226 67 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1956 2523
Size: 1956 567
BtsI GCAGTG_nn'
Cuts at: 0 54 180 284 2288 2523
Size: 54 126 104 2004 235
Fragments arranged by size:
2004 235 126 104 54
EaeI y'GGCC_r
Cuts at: 0 164 524 1410 1457 2523
Size: 164 360 886 47 1066
Fragments arranged by size:
1066 886 360 164 47
EarI CTCTTCn'nnn_
Cuts at: 0 491 839 851 1575 2523
Size: 491 348 12 724 948
Fragments arranged by size:
948 724 491 348 12
EcoICRI GAG'CTC
Cuts at: 0 656 2523
Size: 656 1867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 382 902 1006 1287 1749 2208 2523
Size: 360 22 520 104 281 462 459 315
Fragments arranged by size:
520 462 459 360 315 281 104 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1020 1163 2128 2376 2523
Size: 1020 143 965 248 147
Fragments arranged by size:
1020 965 248 147 143
EcoO109I rG'GnC_Cy
Cuts at: 0 1237 2172 2422 2523
Size: 1237 935 250 101
Fragments arranged by size:
1237 935 250 101
FspI TGC'GCA
Cuts at: 0 349 2280 2523
Size: 349 1931 243
Fragments arranged by size:
1931 349 243
HaeII r_GCGC'y
Cuts at: 0 196 453 1310 1986 2523
Size: 196 257 857 676 537
Fragments arranged by size:
857 676 537 257 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 1789 1821 2101 2133 2523
Size: 299 32 1458 32 280 32 390
Fragments arranged by size:
1458 390 299 280 32 32 32
HincII GTy'rAC
Cuts at: 0 674 2523
Size: 674 1849
HpaI GTT'AAC
Cuts at: 0 674 2523
Size: 674 1849
KpnI G_GTAC'C
Cuts at: 0 1507 2523
Size: 1507 1016
MluI A'CGCG_T
Cuts at: 0 668 2523
Size: 668 1855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 1226 1232 1974 2087 2523
Size: 180 1046 6 742 113 436
Fragments arranged by size:
1046 742 436 180 113 6
MslI CAynn'nnrTG
Cuts at: 0 34 1330 2523
Size: 34 1296 1193
Fragments arranged by size:
1296 1193 34
MspA1I CmG'CkG
Cuts at: 0 90 267 332 970 1496 2523
Size: 90 177 65 638 526 1027
Fragments arranged by size:
1027 638 526 177 90 65
NaeI GCC'GGC
Cuts at: 0 1410 2523
Size: 1410 1113
NcoI C'CATG_G
Cuts at: 0 1217 2204 2523
Size: 1217 987 319
Fragments arranged by size:
1217 987 319
NgoMIV G'CCGG_C
Cuts at: 0 1408 2523
Size: 1408 1115
NsiI A_TGCA'T
Cuts at: 0 735 2095 2523
Size: 735 1360 428
Fragments arranged by size:
1360 735 428
NspI r_CATG'y
Cuts at: 0 173 354 713 2093 2523
Size: 173 181 359 1380 430
Fragments arranged by size:
1380 430 359 181 173
PasI CC'CwG_GG
Cuts at: 0 542 1653 2339 2523
Size: 542 1111 686 184
Fragments arranged by size:
1111 686 542 184
PfoI T'CCnGG_A
Cuts at: 0 770 994 2523
Size: 770 224 1529
Fragments arranged by size:
1529 770 224
PmlI CAC'GTG
Cuts at: 0 1188 2523
Size: 1188 1335
PpuMI rG'GwC_Cy
Cuts at: 0 2422 2523
Size: 2422 101
PspOMI G'GGCC_C
Cuts at: 0 146 2523
Size: 146 2377
PstI C_TGCA'G
Cuts at: 0 389 480 1615 2523
Size: 389 91 1135 908
Fragments arranged by size:
1135 908 389 91
PvuII CAG'CTG
Cuts at: 0 970 1496 2523
Size: 970 526 1027
Fragments arranged by size:
1027 970 526
SacI G_AGCT'C
Cuts at: 0 658 2523
Size: 658 1865
SapI GCTCTTCn'nnn_
Cuts at: 0 1575 2523
Size: 1575 948
SfcI C'TryA_G
Cuts at: 0 385 476 1611 1853 2523
Size: 385 91 1135 242 670
Fragments arranged by size:
1135 670 385 242 91
SmaI CCC'GGG
Cuts at: 0 2402 2523
Size: 2402 121
SmlI C'TyrA_G
Cuts at: 0 796 1020 2523
Size: 796 224 1503
Fragments arranged by size:
1503 796 224
SphI G_CATG'C
Cuts at: 0 173 354 2093 2523
Size: 173 181 1739 430
Fragments arranged by size:
1739 430 181 173
SspI AAT'ATT
Cuts at: 0 181 2523
Size: 181 2342
StuI AGG'CCT
Cuts at: 0 1435 1694 2523
Size: 1435 259 829
Fragments arranged by size:
1435 829 259
StyI C'CwwG_G
Cuts at: 0 647 899 1217 1535 1876 2204 2243 2523
Size: 647 252 318 318 341 328 39 280
Fragments arranged by size:
647 341 328 318 318 280 252 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1389 2236 2361 2523
Size: 1389 847 125 162
Fragments arranged by size:
1389 847 162 125
TatI w'GTAC_w
Cuts at: 0 1072 1712 2488 2523
Size: 1072 640 776 35
Fragments arranged by size:
1072 776 640 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 427 459 1857 1889 2523
Size: 427 32 1398 32 634
Fragments arranged by size:
1398 634 427 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 1215 1394 2523
Size: 69 1146 179 1129
Fragments arranged by size:
1146 1129 179 69
XmaI C'CCGG_G
Cuts at: 0 2400 2523
Size: 2400 123
XmnI GAAnn'nnTTC
Cuts at: 0 139 1885 2523
Size: 139 1746 638
Fragments arranged by size:
1746 638 139
Enzymes that do cut:
Acc65I AcuI AfeI AflII AflIII AhdI AleI AlfI
AlwNI ApaI ApoI AvaI BanI BanII BcgI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII HpaI KpnI MluI
MmeI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PasI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SapI SfcI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AgeI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BciVI BclI BmtI Bpu10I BsaBI BsiEI BsiWI BspMI
BssHII BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoRI EcoRV FalI FseI FspAI
HindIII KasI MfeI MscI NarI NdeI NheI NotI
NruI PacI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SwaI Tth111I XbaI XhoI ZraI