Restriction map for
pF1KB3158
(Linear) (Six-base) MAPSORT of: KIBB3158.seq Check: 7882 from: 1 to: 2772
KIBB3158.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 9 2228 2772
Size: 9 2219 544
Fragments arranged by size:
2219 544 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 258 329 471 1833 2286 2772
Size: 69 189 71 142 1362 453 486
Fragments arranged by size:
1362 486 453 189 142 71 69
AgeI A'CCGG_T
Cuts at: 0 244 2772
Size: 244 2528
AhdI GACnn_n'nnGTC
Cuts at: 0 222 2772
Size: 222 2550
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1070 1102 2772
Size: 1070 32 1670
Fragments arranged by size:
1670 1070 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1915 2772
Size: 1915 857
ApoI r'AATT_y
Cuts at: 0 1685 2691 2772
Size: 1685 1006 81
Fragments arranged by size:
1685 1006 81
AseI AT'TA_AT
Cuts at: 0 641 857 2507 2772
Size: 641 216 1650 265
Fragments arranged by size:
1650 641 265 216
AvaI C'yCGr_G
Cuts at: 0 94 287 2407 2772
Size: 94 193 2120 365
Fragments arranged by size:
2120 365 193 94
AvrII C'CTAG_G
Cuts at: 0 2287 2772
Size: 2287 485
BanI G'GyrC_C
Cuts at: 0 925 2772
Size: 925 1847
BanII G_rGCy'C
Cuts at: 0 2369 2772
Size: 2369 403
BbvCI CC'TCA_GC
Cuts at: 0 794 1999 2772
Size: 794 1205 773
Fragments arranged by size:
1205 794 773
BclI T'GATC_A
Cuts at: 0 2394 2772
Size: 2394 378
BglI GCCn_nnn'nGGC
Cuts at: 0 1792 2772
Size: 1792 980
BglII A'GATC_T
Cuts at: 0 557 2772
Size: 557 2215
Bme1580I G_kGCm'C
Cuts at: 0 928 2772
Size: 928 1844
BmgBI CAC'GTC
Cuts at: 0 696 2772
Size: 696 2076
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1578 2772
Size: 1578 1194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 1111 2772
Size: 1070 41 1661
Fragments arranged by size:
1661 1070 41
Bpu10I CC'TnA_GC
Cuts at: 0 350 794 1999 2772
Size: 350 444 1205 773
Fragments arranged by size:
1205 773 444 350
BsaI GGTCTCn'nnnn_
Cuts at: 0 812 1381 2772
Size: 812 569 1391
Fragments arranged by size:
1391 812 569
BsaAI yAC'GTr
Cuts at: 0 309 2772
Size: 309 2463
BsaBI GATnn'nnATC
Cuts at: 0 511 2772
Size: 511 2261
BsaHI Gr'CG_yC
Cuts at: 0 6 2772
Size: 6 2766
BsaWI w'CCGG_w
Cuts at: 0 244 2772
Size: 244 2528
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 1070 1100 2268 2298 2772
Size: 358 30 682 30 1168 30 474
Fragments arranged by size:
1168 682 474 358 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 414 417 715 1627 2076 2126 2530 2772
Size: 414 3 298 912 449 50 404 242
Fragments arranged by size:
912 449 414 404 298 242 50 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 2772
Size: 322 2450
BsiHKAI G_wGCw'C
Cuts at: 0 161 2772
Size: 161 2611
BsmI GAATG_Cn'
Cuts at: 0 918 2253 2772
Size: 918 1335 519
Fragments arranged by size:
1335 918 519
Bsp1286I G_dGCh'C
Cuts at: 0 161 928 2369 2772
Size: 161 767 1441 403
Fragments arranged by size:
1441 767 403 161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 191 2772
Size: 53 138 2581
Fragments arranged by size:
2581 138 53
BsrBI CCG'CTC
Cuts at: 0 326 407 2772
Size: 326 81 2365
Fragments arranged by size:
2365 326 81
BsrDI GCAATG_nn'
Cuts at: 0 1268 2344 2772
Size: 1268 1076 428
Fragments arranged by size:
1268 1076 428
BsrFI r'CCGG_y
Cuts at: 0 244 2772
Size: 244 2528
BsrGI T'GTAC_A
Cuts at: 0 125 2772
Size: 125 2647
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2430 2625 2772
Size: 2430 195 147
Fragments arranged by size:
2430 195 147
BstYI r'GATC_y
Cuts at: 0 557 1290 2772
Size: 557 733 1482
Fragments arranged by size:
1482 733 557
Bsu36I CC'TnA_GG
Cuts at: 0 476 2772
Size: 476 2296
BtgI C'CryG_G
Cuts at: 0 149 675 2772
Size: 149 526 2097
Fragments arranged by size:
2097 526 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2356 2772
Size: 2356 416
BtsI GCAGTG_nn'
Cuts at: 0 831 1148 2772
Size: 831 317 1624
Fragments arranged by size:
1624 831 317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 800 835 2772
Size: 800 35 1937
Fragments arranged by size:
1937 800 35
EaeI y'GGCC_r
Cuts at: 0 173 300 602 2772
Size: 173 127 302 2170
Fragments arranged by size:
2170 302 173 127
EarI CTCTTCn'nnn_
Cuts at: 0 76 880 1547 1712 2274 2333 2772
Size: 76 804 667 165 562 59 439
Fragments arranged by size:
804 667 562 439 165 76 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 258 329 471 1070 1111 1833 2286
Size: 69 189 71 142 599 41 722 453
Cuts at: 2286 2772
Size: 486
Fragments arranged by size:
722 599 486 453 189 142 71 69
41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 175 475 2772
Size: 136 39 300 2297
Fragments arranged by size:
2297 300 136 39
EcoO109I rG'GnC_Cy
Cuts at: 0 79 180 369 570 1642 2284 2772
Size: 79 101 189 201 1072 642 488
Fragments arranged by size:
1072 642 488 201 189 101 79
EcoRI G'AATT_C
Cuts at: 0 1685 2772
Size: 1685 1087
EcoRV GAT'ATC
Cuts at: 0 2121 2772
Size: 2121 651
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1507 2772
Size: 1507 1265
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 310 342 790 822 960 992
Size: 208 32 70 32 448 32 138 32
Cuts at: 992 1451 1483 1639 1671 1727 1759 2268 2300
Size: 459 32 156 32 56 32 509 32
Cuts at: 2300 2772
Size: 472
Fragments arranged by size:
509 472 459 448 208 156 138 70
56 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1260 1314 1755 2229 2772
Size: 1260 54 441 474 543
Fragments arranged by size:
1260 543 474 441 54
HindIII A'AGCT_T
Cuts at: 0 2607 2772
Size: 2607 165
HpaI GTT'AAC
Cuts at: 0 1260 1314 2772
Size: 1260 54 1458
Fragments arranged by size:
1458 1260 54
MfeI C'AATT_G
Cuts at: 0 864 932 2772
Size: 864 68 1840
Fragments arranged by size:
1840 864 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1787 2772
Size: 1787 985
MscI TGG'CCA
Cuts at: 0 302 604 2772
Size: 302 302 2168
Fragments arranged by size:
2168 302 302
MslI CAynn'nnrTG
Cuts at: 0 1037 1483 2242 2428 2635 2772
Size: 1037 446 759 186 207 137
Fragments arranged by size:
1037 759 446 207 186 137
MspA1I CmG'CkG
Cuts at: 0 89 151 231 940 1707 2772
Size: 89 62 80 709 767 1065
Fragments arranged by size:
1065 767 709 89 80 62
NcoI C'CATG_G
Cuts at: 0 675 2772
Size: 675 2097
NdeI CA'TA_TG
Cuts at: 0 2735 2772
Size: 2735 37
PasI CC'CwG_GG
Cuts at: 0 2678 2772
Size: 2678 94
PflMI CCAn_nnn'nTGG
Cuts at: 0 951 1882 2772
Size: 951 931 890
Fragments arranged by size:
951 931 890
PfoI T'CCnGG_A
Cuts at: 0 572 2772
Size: 572 2200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1070 1102 2772
Size: 1070 32 1670
Fragments arranged by size:
1670 1070 32
PpuMI rG'GwC_Cy
Cuts at: 0 79 180 570 1642 2284 2772
Size: 79 101 390 1072 642 488
Fragments arranged by size:
1072 642 488 390 101 79
PshAI GACnn'nnGTC
Cuts at: 0 443 1823 2772
Size: 443 1380 949
Fragments arranged by size:
1380 949 443
PstI C_TGCA'G
Cuts at: 0 207 2354 2772
Size: 207 2147 418
Fragments arranged by size:
2147 418 207
PvuII CAG'CTG
Cuts at: 0 89 231 940 1707 2772
Size: 89 142 709 767 1065
Fragments arranged by size:
1065 767 709 142 89
SacII CC_GC'GG
Cuts at: 0 152 2772
Size: 152 2620
SalI G'TCGA_C
Cuts at: 0 2227 2772
Size: 2227 545
SanDI GG'GwC_CC
Cuts at: 0 79 570 2772
Size: 79 491 2202
Fragments arranged by size:
2202 491 79
ScaI AGT'ACT
Cuts at: 0 2180 2772
Size: 2180 592
SfcI C'TryA_G
Cuts at: 0 36 203 917 1414 1595 2090 2350 2772
Size: 36 167 714 497 181 495 260 422
Fragments arranged by size:
714 497 495 422 260 181 167 36
SgrAI Cr'CCGG_yG
Cuts at: 0 244 2772
Size: 244 2528
SmaI CCC'GGG
Cuts at: 0 289 2772
Size: 289 2483
SspI AAT'ATT
Cuts at: 0 1378 2769 2772
Size: 1378 1391 3
Fragments arranged by size:
1391 1378 3
StuI AGG'CCT
Cuts at: 0 2292 2772
Size: 2292 480
StyI C'CwwG_G
Cuts at: 0 675 2287 2772
Size: 675 1612 485
Fragments arranged by size:
1612 675 485
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 733 1581 1727 1785 2772
Size: 733 848 146 58 987
Fragments arranged by size:
987 848 733 146 58
TatI w'GTAC_w
Cuts at: 0 125 1730 2178 2700 2772
Size: 125 1605 448 522 72
Fragments arranged by size:
1605 522 448 125 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 358 390 2772
Size: 358 32 2382
Fragments arranged by size:
2382 358 32
Tth111I GACn'n_nGTC
Cuts at: 0 1675 2455 2772
Size: 1675 780 317
Fragments arranged by size:
1675 780 317
XbaI T'CTAG_A
Cuts at: 0 2115 2772
Size: 2115 657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1398 2772
Size: 1398 1374
XmaI C'CCGG_G
Cuts at: 0 287 2772
Size: 287 2485
XmnI GAAnn'nnTTC
Cuts at: 0 1871 2772
Size: 1871 901
Enzymes that do cut:
AccI AcuI AgeI AhdI AloI AlwNI ApoI AseI
AvaI AvrII BanI BanII BbvCI BclI BglI BglII
Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NcoI NdeI PasI
PflMI PfoI PpiI PpuMI PshAI PstI PvuII SacII
SalI SanDI ScaI SfcI SgrAI SmaI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AleI
AlfI ApaI ApaLI AscI AsiSI BaeI BamHI BbeI
BbsI BcgI BciVI BfrBI BlpI BmrI BmtI BpuEI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BssHII BssSI
BstAPI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI
EagI EciI EcoICRI FseI FspI FspAI HaeII KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SapI SbfI SexAI
SfiI SfoI SgfI SmlI SnaBI SpeI SphI SrfI
SwaI XhoI ZraI