(Linear) (Six-base) MAPSORT of: KIBB3129.seq Check: 263 from: 1 to: 717 KIBB3129.orf With 182 enzymes: SgfI * February 28, 2007 11:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 560 717 Size: 560 157 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 396 670 717 Size: 396 274 47 Fragments arranged by size: 396 274 47 AleI CACnn'nnGTG Cuts at: 0 397 717 Size: 397 320 AvaI C'yCGr_G Cuts at: 0 117 157 185 717 Size: 117 40 28 532 Fragments arranged by size: 532 117 40 28 BamHI G'GATC_C Cuts at: 0 563 717 Size: 563 154 BanI G'GyrC_C Cuts at: 0 106 235 264 548 697 717 Size: 106 129 29 284 149 20 Fragments arranged by size: 284 149 129 106 29 20 BanII G_rGCy'C Cuts at: 0 410 717 Size: 410 307 BbeI G_GCGC'C Cuts at: 0 110 239 717 Size: 110 129 478 Fragments arranged by size: 478 129 110 BbsI GAAGACnn'nnnn_ Cuts at: 0 658 717 Size: 658 59 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 73 107 717 Size: 73 34 610 Fragments arranged by size: 610 73 34 BglI GCCn_nnn'nGGC Cuts at: 0 299 717 Size: 299 418 BlpI GC'TnA_GC Cuts at: 0 256 717 Size: 256 461 Bme1580I G_kGCm'C Cuts at: 0 164 717 Size: 164 553 BmrI ACTGGGnnnn_n' Cuts at: 0 438 717 Size: 438 279 BsaHI Gr'CG_yC Cuts at: 0 107 236 364 717 Size: 107 129 128 353 Fragments arranged by size: 353 129 128 107 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 295 325 717 Size: 295 30 392 Fragments arranged by size: 392 295 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 138 418 717 Size: 138 280 299 Fragments arranged by size: 299 280 138 BseYI C'CCAG_C Cuts at: 0 10 152 581 610 717 Size: 10 142 429 29 107 Fragments arranged by size: 429 142 107 29 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 538 717 Size: 340 198 179 Fragments arranged by size: 340 198 179 BsiEI CG_ry'CG Cuts at: 0 197 717 Size: 197 520 BsiHKAI G_wGCw'C Cuts at: 0 410 717 Size: 410 307 Bsp1286I G_dGCh'C Cuts at: 0 164 410 717 Size: 164 246 307 Fragments arranged by size: 307 246 164 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 560 717 Size: 560 157 BsrFI r'CCGG_y Cuts at: 0 298 545 717 Size: 298 247 172 Fragments arranged by size: 298 247 172 BstXI CCAn_nnnn'nTGG Cuts at: 0 399 717 Size: 399 318 BstYI r'GATC_y Cuts at: 0 563 717 Size: 563 154 BtgI C'CryG_G Cuts at: 0 242 392 717 Size: 242 150 325 Fragments arranged by size: 325 242 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 501 717 Size: 501 216 EarI CTCTTCn'nnn_ Cuts at: 0 415 717 Size: 415 302 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 280 717 Size: 280 437 EcoICRI GAG'CTC Cuts at: 0 408 717 Size: 408 309 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 396 670 717 Size: 396 274 47 Fragments arranged by size: 396 274 47 EcoNI CCTnn'n_nnAGG Cuts at: 0 292 717 Size: 292 425 EcoO109I rG'GnC_Cy Cuts at: 0 189 203 247 717 Size: 189 14 44 470 Fragments arranged by size: 470 189 44 14 HaeII r_GCGC'y Cuts at: 0 110 239 717 Size: 110 129 478 Fragments arranged by size: 478 129 110 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 295 327 671 703 717 Size: 295 32 344 32 14 Fragments arranged by size: 344 295 32 32 14 HincII GTy'rAC Cuts at: 0 486 717 Size: 486 231 KasI G'GCGC_C Cuts at: 0 106 235 717 Size: 106 129 482 Fragments arranged by size: 482 129 106 MslI CAynn'nnrTG Cuts at: 0 397 472 717 Size: 397 75 245 Fragments arranged by size: 397 245 75 MspA1I CmG'CkG Cuts at: 0 213 244 354 717 Size: 213 31 110 363 Fragments arranged by size: 363 213 110 31 NaeI GCC'GGC Cuts at: 0 300 717 Size: 300 417 NarI GG'CG_CC Cuts at: 0 107 236 717 Size: 107 129 481 Fragments arranged by size: 481 129 107 NgoMIV G'CCGG_C Cuts at: 0 298 717 Size: 298 419 NspI r_CATG'y Cuts at: 0 618 717 Size: 618 99 PasI CC'CwG_GG Cuts at: 0 693 717 Size: 693 24 PfoI T'CCnGG_A Cuts at: 0 566 717 Size: 566 151 PpuMI rG'GwC_Cy Cuts at: 0 189 203 717 Size: 189 14 514 Fragments arranged by size: 514 189 14 PsiI TTA'TAA Cuts at: 0 62 717 Size: 62 655 PstI C_TGCA'G Cuts at: 0 218 717 Size: 218 499 PvuII CAG'CTG Cuts at: 0 354 717 Size: 354 363 SacI G_AGCT'C Cuts at: 0 410 717 Size: 410 307 SacII CC_GC'GG Cuts at: 0 245 717 Size: 245 472 SanDI GG'GwC_CC Cuts at: 0 189 717 Size: 189 528 SapI GCTCTTCn'nnn_ Cuts at: 0 415 717 Size: 415 302 SfcI C'TryA_G Cuts at: 0 214 717 Size: 214 503 SfoI GGC'GCC Cuts at: 0 108 237 717 Size: 108 129 480 Fragments arranged by size: 480 129 108 SmaI CCC'GGG Cuts at: 0 119 159 187 717 Size: 119 40 28 530 Fragments arranged by size: 530 119 40 28 SphI G_CATG'C Cuts at: 0 618 717 Size: 618 99 SrfI GCCC'GGGC Cuts at: 0 159 717 Size: 159 558 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 685 717 Size: 685 32 TatI w'GTAC_w Cuts at: 0 50 717 Size: 50 667 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 156 188 260 292 717 Size: 156 32 72 32 425 Fragments arranged by size: 425 156 72 32 32 Tth111I GACn'n_nGTC Cuts at: 0 193 717 Size: 193 524 XmaI C'CCGG_G Cuts at: 0 117 157 185 717 Size: 117 40 28 532 Fragments arranged by size: 532 117 40 28 Enzymes that do cut: AarI AcuI AleI AvaI BamHI BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmrI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstXI BstYI BtgI BtgZI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MslI MspA1I NaeI NarI NgoMIV NspI PasI PfoI PpuMI PsiI PstI PvuII SacI SacII SanDI SapI SfcI SfoI SmaI SphI SrfI TaqII TatI TstI Tth111I XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BglII BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SspI StuI StyI SwaI XbaI XcmI XhoI XmnI ZraI