(Linear) (Six-base) MAPSORT of: KIBB3122.seq  Check: 9302  from: 1  to: 654



KIBB3122.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:      0     333     654 
   Size:        333     321
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     368     654 
   Size:        368     286
 
AlwNI CAG_nnn'CTG

Cuts at:      0     346     654 
   Size:        346     308
 
ApoI r'AATT_y

Cuts at:      0     118     654 
   Size:        118     536
 
BanI G'GyrC_C

Cuts at:      0     630     654 
   Size:        630      24
 
BbvCI CC'TCA_GC

Cuts at:      0     368     654 
   Size:        368     286
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     324     419     654 
   Size:        324      95     235

  Fragments arranged by size: 

                 324     235      95
 
Bpu10I CC'TnA_GC

Cuts at:      0     368     654 
   Size:        368     286
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     561     654 
   Size:        561      93
 
BsiHKAI G_wGCw'C

Cuts at:      0     187     654 
   Size:        187     467
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     480     654 
   Size:        480     174
 
Bsp1286I G_dGCh'C

Cuts at:      0     187     654 
   Size:        187     467
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     548     654 
   Size:        548     106
 
BsrGI T'GTAC_A

Cuts at:      0     124     654 
   Size:        124     530
 
BstEII G'GTnAC_C

Cuts at:      0     639     654 
   Size:        639      15
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     627     654 
   Size:        627      27
 
BtsI GCAGTG_nn'

Cuts at:      0     429     654 
   Size:        429     225
 
EaeI y'GGCC_r

Cuts at:      0     528     654 
   Size:        528     126
 
EarI CTCTTCn'nnn_

Cuts at:      0     277     654 
   Size:        277     377
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     324     368     419     654 
   Size:        324      44      51     235

  Fragments arranged by size: 

                 324     235      51      44
 
EcoRI G'AATT_C

Cuts at:      0     118     654 
   Size:        118     536
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     164     654 
   Size:        164     490
 
HaeII r_GCGC'y

Cuts at:      0     465     654 
   Size:        465     189
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     460     492     654 
   Size:        460      32     162

  Fragments arranged by size: 

                 460     162      32
 
HincII GTy'rAC

Cuts at:      0     600     654 
   Size:        600      54
 
HindIII A'AGCT_T

Cuts at:      0     146     654 
   Size:        146     508
 
MfeI C'AATT_G

Cuts at:      0     492     654 
   Size:        492     162
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     629     654 
   Size:        629      25
 
MscI TGG'CCA

Cuts at:      0     530     654 
   Size:        530     124
 
MslI CAynn'nnrTG

Cuts at:      0     625     654 
   Size:        625      29
 
PstI C_TGCA'G

Cuts at:      0     545     654 
   Size:        545     109
 
SfcI C'TryA_G

Cuts at:      0     541     654 
   Size:        541     113
 
SspI AAT'ATT

Cuts at:      0     381     654 
   Size:        381     273
 
TatI w'GTAC_w

Cuts at:      0     124     654 
   Size:        124     530
 
Tth111I GACn'n_nGTC

Cuts at:      0     595     654 
   Size:        595      59
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     BanI    BbvCI     BpmI   Bpu10I
    BseRI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrGI   BstEII    BstXI
     BtsI     EaeI     EarI  Eco57MI    EcoRI     FalI    HaeII    Hin4I
   HincII  HindIII     MfeI     MmeI     MscI     MslI     PstI     SfcI
     SspI     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FseI     FspI    FspAI     HpaI     KasI     KpnI     MluI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI