(Linear) (Six-base) MAPSORT of: KIBB3105.seq  Check: 5386  from: 1  to: 492



KIBB3105.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

ApoI r'AATT_y

Cuts at:      0     154     492 
   Size:        154     338
 
AvaI C'yCGr_G

Cuts at:      0     167     299     419     492 
   Size:        167     132     120      73

  Fragments arranged by size: 

                 167     132     120      73
 
BfrBI ATG'CAT

Cuts at:      0       3     492 
   Size:          3     489
 
BglII A'GATC_T

Cuts at:      0     340     492 
   Size:        340     152
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     341     492 
   Size:        341     151
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     464     492 
   Size:        464      28
 
BsaBI GATnn'nnATC

Cuts at:      0     308     492 
   Size:        308     184
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     333     492 
   Size:        333     159
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     368     492 
   Size:        368     124
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     181     492 
   Size:        181     311
 
BstYI r'GATC_y

Cuts at:      0     162     340     492 
   Size:        162     178     152

  Fragments arranged by size: 

                 178     162     152
 
DraIII CAC_nnn'GTG

Cuts at:      0     214     492 
   Size:        214     278
 
EarI CTCTTCn'nnn_

Cuts at:      0     396     492 
   Size:        396      96
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     453     492 
   Size:        453      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     380     492 
   Size:        380     112
 
HindIII A'AGCT_T

Cuts at:      0     357     492 
   Size:        357     135
 
MspA1I CmG'CkG

Cuts at:      0     178     492 
   Size:        178     314
 
NsiI A_TGCA'T

Cuts at:      0       5     492 
   Size:          5     487
 
PpuMI rG'GwC_Cy

Cuts at:      0     380     492 
   Size:        380     112
 
PvuII CAG'CTG

Cuts at:      0     178     492 
   Size:        178     314
 
SmlI C'TyrA_G

Cuts at:      0     299     492 
   Size:        299     193
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     424     456     492 
   Size:        424      32      36

  Fragments arranged by size: 

                 424      36      32
 
XbaI T'CTAG_A

Cuts at:      0     388     492 
   Size:        388     104
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     281     492 
   Size:        281     211
 
XhoI C'TCGA_G

Cuts at:      0     299     492 
   Size:        299     193
 

 Enzymes that do cut:

     ApoI     AvaI    BfrBI    BglII     BplI     BsaI    BsaBI    BseRI
    BspMI   BstAPI    BstYI   DraIII     EarI    EcoNI EcoO109I  HindIII
   MspA1I     NsiI    PpuMI    PvuII     SmlI     TstI     XbaI     XcmI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XmaI     XmnI     ZraI