Restriction map for
pF1KB3060
(Linear) (Six-base) MAPSORT of: KIBB3060.seq Check: 9017 from: 1 to: 1878
KIBB3060.orf
With 182 enzymes: SgfI *
April 17, 2013 10:25 ..
AclI AA'CG_TT
Cuts at: 0 1766 1878
Size: 1766 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 1695 1878
Size: 927 768 183
Fragments arranged by size:
927 768 183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 343 385 1878
Size: 343 42 1493
Fragments arranged by size:
1493 343 42
AlwNI CAG_nnn'CTG
Cuts at: 0 18 471 753 1249 1878
Size: 18 453 282 496 629
Fragments arranged by size:
629 496 453 282 18
AvaI C'yCGr_G
Cuts at: 0 1175 1878
Size: 1175 703
BamHI G'GATC_C
Cuts at: 0 1082 1878
Size: 1082 796
BanI G'GyrC_C
Cuts at: 0 180 856 928 1878
Size: 180 676 72 950
Fragments arranged by size:
950 676 180 72
BanII G_rGCy'C
Cuts at: 0 1352 1447 1878
Size: 1352 95 431
Fragments arranged by size:
1352 431 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 1057 1245 1384 1701 1878
Size: 1057 188 139 317 177
Fragments arranged by size:
1057 317 188 177 139
BbvCI CC'TCA_GC
Cuts at: 0 482 1878
Size: 482 1396
BciVI GTATCCnnnnn_n'
Cuts at: 0 911 1878
Size: 911 967
BclI T'GATC_A
Cuts at: 0 1869 1878
Size: 1869 9
BfrBI ATG'CAT
Cuts at: 0 1526 1878
Size: 1526 352
Bme1580I G_kGCm'C
Cuts at: 0 647 1724 1878
Size: 647 1077 154
Fragments arranged by size:
1077 647 154
BmrI ACTGGGnnnn_n'
Cuts at: 0 150 1114 1348 1639 1878
Size: 150 964 234 291 239
Fragments arranged by size:
964 291 239 234 150
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1047 1834 1878
Size: 1047 787 44
Fragments arranged by size:
1047 787 44
Bpu10I CC'TnA_GC
Cuts at: 0 482 808 1486 1878
Size: 482 326 678 392
Fragments arranged by size:
678 482 392 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 1018 1878
Size: 1018 860
BsaAI yAC'GTr
Cuts at: 0 117 1361 1878
Size: 117 1244 517
Fragments arranged by size:
1244 517 117
BsaBI GATnn'nnATC
Cuts at: 0 445 1878
Size: 445 1433
BsaWI w'CCGG_w
Cuts at: 0 29 215 1878
Size: 29 186 1663
Fragments arranged by size:
1663 186 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1751 1781 1878
Size: 1751 30 97
Fragments arranged by size:
1751 97 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1258 1878
Size: 1258 620
BseYI C'CCAG_C
Cuts at: 0 91 1199 1563 1839 1878
Size: 91 1108 364 276 39
Fragments arranged by size:
1108 364 276 91 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1600 1617 1878
Size: 1600 17 261
Fragments arranged by size:
1600 261 17
BsiHKAI G_wGCw'C
Cuts at: 0 1334 1447 1878
Size: 1334 113 431
Fragments arranged by size:
1334 431 113
Bsp1286I G_dGCh'C
Cuts at: 0 647 1334 1352 1447 1724 1878
Size: 647 687 18 95 277 154
Fragments arranged by size:
687 647 277 154 95 18
BspEI T'CCGG_A
Cuts at: 0 29 1878
Size: 29 1849
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 317 1036 1878
Size: 317 719 842
Fragments arranged by size:
842 719 317
BsrBI CCG'CTC
Cuts at: 0 60 1878
Size: 60 1818
BsrDI GCAATG_nn'
Cuts at: 0 320 1878
Size: 320 1558
BsrFI r'CCGG_y
Cuts at: 0 183 1878
Size: 183 1695
BssSI C'ACGA_G
Cuts at: 0 105 496 1531 1878
Size: 105 391 1035 347
Fragments arranged by size:
1035 391 347 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 18 1878
Size: 18 1860
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 435 540 1878
Size: 228 207 105 1338
Fragments arranged by size:
1338 228 207 105
BstYI r'GATC_y
Cuts at: 0 1082 1878
Size: 1082 796
BtgI C'CryG_G
Cuts at: 0 293 434 1501 1878
Size: 293 141 1067 377
Fragments arranged by size:
1067 377 293 141
BtsI GCAGTG_nn'
Cuts at: 0 161 1676 1722 1878
Size: 161 1515 46 156
Fragments arranged by size:
1515 161 156 46
DraI TTT'AAA
Cuts at: 0 1020 1878
Size: 1020 858
DraIII CAC_nnn'GTG
Cuts at: 0 1655 1878
Size: 1655 223
EaeI y'GGCC_r
Cuts at: 0 344 1793 1878
Size: 344 1449 85
Fragments arranged by size:
1449 344 85
EarI CTCTTCn'nnn_
Cuts at: 0 515 584 946 1072 1276 1825 1878
Size: 515 69 362 126 204 549 53
Fragments arranged by size:
549 515 362 204 126 69 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 250 1878
Size: 250 1628
EcoICRI GAG'CTC
Cuts at: 0 1445 1878
Size: 1445 433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 1695 1878
Size: 927 768 183
Fragments arranged by size:
927 768 183
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 1878
Size: 10 1868
EcoO109I rG'GnC_Cy
Cuts at: 0 15 770 1878
Size: 15 755 1108
Fragments arranged by size:
1108 755 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1314 1878
Size: 1314 564
FspI TGC'GCA
Cuts at: 0 331 1878
Size: 331 1547
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1017 1049 1878
Size: 1017 32 829
Fragments arranged by size:
1017 829 32
HincII GTy'rAC
Cuts at: 0 1764 1878
Size: 1764 114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 440 536 620 1878
Size: 128 312 96 84 1258
Fragments arranged by size:
1258 312 128 96 84
MscI TGG'CCA
Cuts at: 0 1795 1878
Size: 1795 83
MslI CAynn'nnrTG
Cuts at: 0 280 433 439 616 970 1153 1531 1878
Size: 280 153 6 177 354 183 378 347
Fragments arranged by size:
378 354 347 280 183 177 153 6
MspA1I CmG'CkG
Cuts at: 0 980 1878
Size: 980 898
NaeI GCC'GGC
Cuts at: 0 185 1878
Size: 185 1693
NcoI C'CATG_G
Cuts at: 0 434 1878
Size: 434 1444
NgoMIV G'CCGG_C
Cuts at: 0 183 1878
Size: 183 1695
NsiI A_TGCA'T
Cuts at: 0 1528 1878
Size: 1528 350
NspI r_CATG'y
Cuts at: 0 366 1526 1878
Size: 366 1160 352
Fragments arranged by size:
1160 366 352
PasI CC'CwG_GG
Cuts at: 0 1388 1878
Size: 1388 490
PflMI CCAn_nnn'nTGG
Cuts at: 0 1388 1878
Size: 1388 490
PfoI T'CCnGG_A
Cuts at: 0 878 1878
Size: 878 1000
PmlI CAC'GTG
Cuts at: 0 1361 1878
Size: 1361 517
PpuMI rG'GwC_Cy
Cuts at: 0 770 1878
Size: 770 1108
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 748 780 1878
Size: 748 32 1098
Fragments arranged by size:
1098 748 32
PstI C_TGCA'G
Cuts at: 0 14 174 467 1878
Size: 14 160 293 1411
Fragments arranged by size:
1411 293 160 14
PvuII CAG'CTG
Cuts at: 0 980 1878
Size: 980 898
SacI G_AGCT'C
Cuts at: 0 1447 1878
Size: 1447 431
ScaI AGT'ACT
Cuts at: 0 241 1878
Size: 241 1637
SexAI A'CCwGG_T
Cuts at: 0 931 1878
Size: 931 947
SfcI C'TryA_G
Cuts at: 0 10 170 463 1878
Size: 10 160 293 1415
Fragments arranged by size:
1415 293 160 10
SmaI CCC'GGG
Cuts at: 0 1177 1878
Size: 1177 701
SphI G_CATG'C
Cuts at: 0 366 1526 1878
Size: 366 1160 352
Fragments arranged by size:
1160 366 352
StuI AGG'CCT
Cuts at: 0 103 1878
Size: 103 1775
StyI C'CwwG_G
Cuts at: 0 434 1257 1878
Size: 434 823 621
Fragments arranged by size:
823 621 434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 132 716 1360 1878
Size: 132 584 644 518
Fragments arranged by size:
644 584 518 132
TatI w'GTAC_w
Cuts at: 0 137 239 764 1878
Size: 137 102 525 1114
Fragments arranged by size:
1114 525 137 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 432 1878
Size: 261 171 1446
Fragments arranged by size:
1446 261 171
XmaI C'CCGG_G
Cuts at: 0 1175 1878
Size: 1175 703
XmnI GAAnn'nnTTC
Cuts at: 0 1369 1878
Size: 1369 509
Enzymes that do cut:
AclI AcuI AlfI AlwNI AvaI BamHI BanI BanII
BbsI BbvCI BciVI BclI BfrBI Bme1580I BmrI BplI
Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtsI DraI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NsiI NspI PasI PflMI PfoI PmlI
PpuMI PsrI PstI PvuII SacI ScaI SexAI SfcI
SmaI SphI StuI StyI TaqII TatI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BglI BglII BlpI
BmgBI BmtI BpmI BpuEI BsaHI BsiEI BsiWI BsmI
BsmBI BspHI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EagI EcoRI EcoRV FseI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI PacI PciI PmeI
PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XbaI XhoI ZraI