Restriction map for
pF1KB0968
(Linear) (Six-base) MAPSORT of: KIBB0968.seq Check: 4804 from: 1 to: 1293
KIBB0968.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 237 1072 1293
Size: 237 835 221
Fragments arranged by size:
835 237 221
AccI GT'mk_AC
Cuts at: 0 374 1293
Size: 374 919
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1293
Size: 952 341
AfeI AGC'GCT
Cuts at: 0 1084 1293
Size: 1084 209
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1042 1074 1293
Size: 1042 32 219
Fragments arranged by size:
1042 219 32
AlwNI CAG_nnn'CTG
Cuts at: 0 856 1293
Size: 856 437
ApaI G_GGCC'C
Cuts at: 0 305 659 1293
Size: 305 354 634
Fragments arranged by size:
634 354 305
ApoI r'AATT_y
Cuts at: 0 76 1293
Size: 76 1217
AvaI C'yCGr_G
Cuts at: 0 1046 1140 1293
Size: 1046 94 153
Fragments arranged by size:
1046 153 94
BanI G'GyrC_C
Cuts at: 0 310 1293
Size: 310 983
BanII G_rGCy'C
Cuts at: 0 221 305 659 1293
Size: 221 84 354 634
Fragments arranged by size:
634 354 221 84
BglI GCCn_nnn'nGGC
Cuts at: 0 342 1293
Size: 342 951
BlpI GC'TnA_GC
Cuts at: 0 560 1293
Size: 560 733
Bme1580I G_kGCm'C
Cuts at: 0 305 338 347 659 1293
Size: 305 33 9 312 634
Fragments arranged by size:
634 312 305 33 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 362 770 1148 1293
Size: 362 408 378 145
Fragments arranged by size:
408 378 362 145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1022 1293
Size: 1022 271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 227 509 1042 1277 1293
Size: 24 203 282 533 235 16
Fragments arranged by size:
533 282 235 203 24 16
Bpu10I CC'TnA_GC
Cuts at: 0 1105 1268 1293
Size: 1105 163 25
Fragments arranged by size:
1105 163 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1293
Size: 37 1256
BsaBI GATnn'nnATC
Cuts at: 0 188 1293
Size: 188 1105
BsaHI Gr'CG_yC
Cuts at: 0 4 1213 1293
Size: 4 1209 80
Fragments arranged by size:
1209 80 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 450 480 1042 1072 1293
Size: 22 30 398 30 562 30 221
Fragments arranged by size:
562 398 221 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 792 1213 1293
Size: 34 758 421 80
Fragments arranged by size:
758 421 80 34
BseYI C'CCAG_C
Cuts at: 0 578 630 659 840 856 1170 1293
Size: 578 52 29 181 16 314 123
Fragments arranged by size:
578 314 181 123 52 29 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 1293
Size: 90 1203
Bsp1286I G_dGCh'C
Cuts at: 0 221 305 338 347 659 1293
Size: 221 84 33 9 312 634
Fragments arranged by size:
634 312 221 84 33 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 237 1072 1293
Size: 237 835 221
Fragments arranged by size:
835 237 221
BsrBI CCG'CTC
Cuts at: 0 837 1293
Size: 837 456
BstAPI GCAn_nnn'nTGC
Cuts at: 0 669 1293
Size: 669 624
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 177 322 526 699 895 1170 1293
Size: 48 129 145 204 173 196 275 123
Fragments arranged by size:
275 204 196 173 145 129 123 48
Bsu36I CC'TnA_GG
Cuts at: 0 255 1293
Size: 255 1038
BtgI C'CryG_G
Cuts at: 0 176 469 745 1125 1293
Size: 176 293 276 380 168
Fragments arranged by size:
380 293 276 176 168
BtsI GCAGTG_nn'
Cuts at: 0 159 541 704 792 1293
Size: 159 382 163 88 501
Fragments arranged by size:
501 382 163 159 88
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 63 98 1293
Size: 63 35 1195
Fragments arranged by size:
1195 63 35
DraIII CAC_nnn'GTG
Cuts at: 0 751 1293
Size: 751 542
EaeI y'GGCC_r
Cuts at: 0 50 1293
Size: 50 1243
EarI CTCTTCn'nnn_
Cuts at: 0 357 991 1190 1293
Size: 357 634 199 103
Fragments arranged by size:
634 357 199 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 227 509 952 1042 1277 1293
Size: 24 203 282 443 90 235 16
Fragments arranged by size:
443 282 235 203 90 24 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 16 1293
Size: 16 1277
EcoO109I rG'GnC_Cy
Cuts at: 0 318 655 656 965 1144 1293
Size: 318 337 1 309 179 149
Fragments arranged by size:
337 318 309 179 149 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 957 1293
Size: 957 336
FspI TGC'GCA
Cuts at: 0 1036 1293
Size: 1036 257
FspAI rTGC'GCAy
Cuts at: 0 1036 1293
Size: 1036 257
HaeII r_GCGC'y
Cuts at: 0 801 1086 1293
Size: 801 285 207
Fragments arranged by size:
801 285 207
MscI TGG'CCA
Cuts at: 0 52 1293
Size: 52 1241
MslI CAynn'nnrTG
Cuts at: 0 401 1293
Size: 401 892
MspA1I CmG'CkG
Cuts at: 0 582 853 884 1293
Size: 582 271 31 409
Fragments arranged by size:
582 409 271 31
NcoI C'CATG_G
Cuts at: 0 176 469 1293
Size: 176 293 824
Fragments arranged by size:
824 293 176
NspI r_CATG'y
Cuts at: 0 667 1293
Size: 667 626
PasI CC'CwG_GG
Cuts at: 0 298 322 1293
Size: 298 24 971
Fragments arranged by size:
971 298 24
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1042 1074 1293
Size: 1042 32 219
Fragments arranged by size:
1042 219 32
PpuMI rG'GwC_Cy
Cuts at: 0 318 1144 1293
Size: 318 826 149
Fragments arranged by size:
826 318 149
PspOMI G'GGCC_C
Cuts at: 0 301 655 1293
Size: 301 354 638
Fragments arranged by size:
638 354 301
PstI C_TGCA'G
Cuts at: 0 234 1293
Size: 234 1059
PvuII CAG'CTG
Cuts at: 0 582 884 1293
Size: 582 302 409
Fragments arranged by size:
582 409 302
SanDI GG'GwC_CC
Cuts at: 0 1144 1293
Size: 1144 149
SapI GCTCTTCn'nnn_
Cuts at: 0 357 991 1293
Size: 357 634 302
Fragments arranged by size:
634 357 302
SbfI CC_TGCA'GG
Cuts at: 0 234 1293
Size: 234 1059
SfcI C'TryA_G
Cuts at: 0 230 1293
Size: 230 1063
SmaI CCC'GGG
Cuts at: 0 1048 1142 1293
Size: 1048 94 151
Fragments arranged by size:
1048 151 94
SmlI C'TyrA_G
Cuts at: 0 16 1293
Size: 16 1277
SpeI A'CTAG_T
Cuts at: 0 330 1293
Size: 330 963
StyI C'CwwG_G
Cuts at: 0 176 338 469 692 1163 1293
Size: 176 162 131 223 471 130
Fragments arranged by size:
471 223 176 162 131 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 264 328 1138 1293
Size: 264 64 810 155
Fragments arranged by size:
810 264 155 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 22 54 450 482 1293
Size: 22 32 396 32 811
Fragments arranged by size:
811 396 32 32 22
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 295 1293
Size: 295 998
XmaI C'CCGG_G
Cuts at: 0 1046 1140 1293
Size: 1046 94 153
Fragments arranged by size:
1046 153 94
XmnI GAAnn'nnTTC
Cuts at: 0 1057 1293
Size: 1057 236
Enzymes that do cut:
AarI AccI AcuI AfeI AloI AlwNI ApaI ApoI
AvaI BanI BanII BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BseYI
BsgI Bsp1286I BspMI BsrBI BstAPI BstXI Bsu36I BtgI
BtsI CspCI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
FalI FspI FspAI HaeII MscI MslI MspA1I NcoI
NspI PasI PpiI PpuMI PspOMI PstI PvuII SanDI
SapI SbfI SfcI SmaI SmlI SpeI StyI TaqII
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BsaI BsaAI BsaWI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI DraI
DrdI EagI EciI EcoICRI EcoRI EcoRV FseI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI ZraI