Restriction map for
pF1KB0960
(Linear) (Six-base) MAPSORT of: KIBB0960.seq Check: 1386 from: 1 to: 1275
KIBB0960.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 0 968 1275
Size: 968 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 103 703 1275
Size: 43 60 600 572
Fragments arranged by size:
600 572 60 43
AfeI AGC'GCT
Cuts at: 0 163 1275
Size: 163 1112
AhdI GACnn_n'nnGTC
Cuts at: 0 852 1275
Size: 852 423
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 182 1275
Size: 182 1093
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 1275
Size: 1138 32 105
Fragments arranged by size:
1138 105 32
ApoI r'AATT_y
Cuts at: 0 645 1275
Size: 645 630
AvaI C'yCGr_G
Cuts at: 0 146 579 842 1142 1275
Size: 146 433 263 300 133
Fragments arranged by size:
433 300 263 146 133
BanI G'GyrC_C
Cuts at: 0 35 266 367 1275
Size: 35 231 101 908
Fragments arranged by size:
908 231 101 35
BanII G_rGCy'C
Cuts at: 0 147 949 1275
Size: 147 802 326
Fragments arranged by size:
802 326 147
BbeI G_GCGC'C
Cuts at: 0 270 1275
Size: 270 1005
BbsI GAAGACnn'nnnn_
Cuts at: 0 709 1275
Size: 709 566
BciVI GTATCCnnnnn_n'
Cuts at: 0 985 1275
Size: 985 290
BlpI GC'TnA_GC
Cuts at: 0 95 1275
Size: 95 1180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1195 1219 1273 1275
Size: 1195 24 54 2
Fragments arranged by size:
1195 54 24 2
Bpu10I CC'TnA_GC
Cuts at: 0 1100 1275
Size: 1100 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1072 1275
Size: 1072 203
BsaI GGTCTCn'nnnn_
Cuts at: 0 5 860 1275
Size: 5 855 415
Fragments arranged by size:
855 415 5
BsaHI Gr'CG_yC
Cuts at: 0 267 1275
Size: 267 1008
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 52 82 1275
Size: 52 30 1193
Fragments arranged by size:
1193 52 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 316 451 454 777 1081 1084 1275
Size: 127 189 135 3 323 304 3 191
Fragments arranged by size:
323 304 191 189 135 127 3 3
BseYI C'CCAG_C
Cuts at: 0 261 892 1275
Size: 261 631 383
Fragments arranged by size:
631 383 261
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 886 1275
Size: 638 248 389
Fragments arranged by size:
638 389 248
BsiHKAI G_wGCw'C
Cuts at: 0 614 826 1275
Size: 614 212 449
Fragments arranged by size:
614 449 212
Bsp1286I G_dGCh'C
Cuts at: 0 147 614 826 949 1275
Size: 147 467 212 123 326
Fragments arranged by size:
467 326 212 147 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 1275
Size: 266 1009
BsrBI CCG'CTC
Cuts at: 0 99 1275
Size: 99 1176
BstXI CCAn_nnnn'nTGG
Cuts at: 0 229 1275
Size: 229 1046
BstYI r'GATC_y
Cuts at: 0 398 775 1275
Size: 398 377 500
Fragments arranged by size:
500 398 377
Bsu36I CC'TnA_GG
Cuts at: 0 49 1275
Size: 49 1226
EaeI y'GGCC_r
Cuts at: 0 389 1275
Size: 389 886
EarI CTCTTCn'nnn_
Cuts at: 0 22 131 146 327 421 814 1275
Size: 22 109 15 181 94 393 461
Fragments arranged by size:
461 393 181 109 94 22 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 23 1275
Size: 23 1252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 103 703 1195 1219 1273 1275
Size: 43 60 600 492 24 54 2
Fragments arranged by size:
600 492 60 54 43 24 2
EcoO109I rG'GnC_Cy
Cuts at: 0 226 408 724 839 1275
Size: 226 182 316 115 436
Fragments arranged by size:
436 316 226 182 115
HaeII r_GCGC'y
Cuts at: 0 165 270 1275
Size: 165 105 1005
Fragments arranged by size:
1005 165 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 573 605 838 870 1067 1099 1168 1200
Size: 573 32 233 32 197 32 69 32
Cuts at: 1200 1275
Size: 75
Fragments arranged by size:
573 233 197 75 69 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 266 1275
Size: 266 1009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1275
Size: 188 1087
MscI TGG'CCA
Cuts at: 0 391 1275
Size: 391 884
MspA1I CmG'CkG
Cuts at: 0 123 1275
Size: 123 1152
NarI GG'CG_CC
Cuts at: 0 267 1275
Size: 267 1008
NspI r_CATG'y
Cuts at: 0 811 985 1099 1275
Size: 811 174 114 176
Fragments arranged by size:
811 176 174 114
PasI CC'CwG_GG
Cuts at: 0 864 1275
Size: 864 411
PfoI T'CCnGG_A
Cuts at: 0 401 987 1275
Size: 401 586 288
Fragments arranged by size:
586 401 288
PpuMI rG'GwC_Cy
Cuts at: 0 226 408 724 839 1275
Size: 226 182 316 115 436
Fragments arranged by size:
436 316 226 182 115
PspXI vC'TCGA_Gb
Cuts at: 0 579 1275
Size: 579 696
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 576 608 1275
Size: 576 32 667
Fragments arranged by size:
667 576 32
PstI C_TGCA'G
Cuts at: 0 1014 1275
Size: 1014 261
SanDI GG'GwC_CC
Cuts at: 0 839 1275
Size: 839 436
SapI GCTCTTCn'nnn_
Cuts at: 0 22 327 814 1275
Size: 22 305 487 461
Fragments arranged by size:
487 461 305 22
SfcI C'TryA_G
Cuts at: 0 1010 1275
Size: 1010 265
SfoI GGC'GCC
Cuts at: 0 268 1275
Size: 268 1007
SmaI CCC'GGG
Cuts at: 0 148 844 1144 1275
Size: 148 696 300 131
Fragments arranged by size:
696 300 148 131
SmlI C'TyrA_G
Cuts at: 0 579 1087 1275
Size: 579 508 188
Fragments arranged by size:
579 508 188
SphI G_CATG'C
Cuts at: 0 1099 1275
Size: 1099 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 52 84 1275
Size: 52 32 1191
Fragments arranged by size:
1191 52 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 229 1275
Size: 229 1046
XhoI C'TCGA_G
Cuts at: 0 579 1275
Size: 579 696
XmaI C'CCGG_G
Cuts at: 0 146 842 1142 1275
Size: 146 696 300 133
Fragments arranged by size:
696 300 146 133
Enzymes that do cut:
AccI AcuI AfeI AhdI AlfI AloI ApoI AvaI
BanI BanII BbeI BbsI BciVI BlpI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BstXI BstYI Bsu36I EaeI EarI
EciI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI
MspA1I NarI NspI PasI PfoI PpuMI PspXI PsrI
PstI SanDI SapI SfcI SfoI SmaI SmlI SphI
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AleI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BfrBI BglI BglII Bme1580I
BmgBI BmrI BmtI BplI BsaAI BsaBI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PvuI
PvuII RsrII SacI SacII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
StyI SwaI TaqII TatI Tth111I XbaI XmnI ZraI