(Linear) (Six-base) MAPSORT of: KIBB0958.seq  Check: 9786  from: 1  to: 1260



KIBB0958.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781     970    1260 
   Size:        781     189     290

  Fragments arranged by size: 

                 781     290     189
 
AflII C'TTAA_G

Cuts at:      0    1231    1260 
   Size:       1231      29
 
AleI CACnn'nnGTG

Cuts at:      0     611    1260 
   Size:        611     649
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1009    1260 
   Size:       1009     251
 
ApoI r'AATT_y

Cuts at:      0     130     620    1260 
   Size:        130     490     640

  Fragments arranged by size: 

                 640     490     130
 
AvaI C'yCGr_G

Cuts at:      0      28    1260 
   Size:         28    1232
 
BanII G_rGCy'C

Cuts at:      0     958    1260 
   Size:        958     302
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     524    1159    1260 
   Size:        524     635     101

  Fragments arranged by size: 

                 635     524     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     877     895    1260 
   Size:        877      18     365

  Fragments arranged by size: 

                 877     365      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     302    1260 
   Size:        302     958
 
BsaWI w'CCGG_w

Cuts at:      0      50    1260 
   Size:         50    1210
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     348     378    1260 
   Size:        348      30     882

  Fragments arranged by size: 

                 882     348      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     369    1117    1260 
   Size:        369     748     143

  Fragments arranged by size: 

                 748     369     143
 
BseYI C'CCAG_C

Cuts at:      0     285     716    1260 
   Size:        285     431     544

  Fragments arranged by size: 

                 544     431     285
 
BsiHKAI G_wGCw'C

Cuts at:      0     812    1260 
   Size:        812     448
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     469    1260 
   Size:        469     791
 
Bsp1286I G_dGCh'C

Cuts at:      0     812     958    1260 
   Size:        812     146     302

  Fragments arranged by size: 

                 812     302     146
 
BspHI T'CATG_A

Cuts at:      0     401     753    1260 
   Size:        401     352     507

  Fragments arranged by size: 

                 507     401     352
 
BtgI C'CryG_G

Cuts at:      0     907    1260 
   Size:        907     353
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      78     113    1260 
   Size:         78      35    1147

  Fragments arranged by size: 

                1147      78      35
 
EaeI y'GGCC_r

Cuts at:      0     410    1260 
   Size:        410     850
 
EarI CTCTTCn'nnn_

Cuts at:      0     946    1085    1231    1260 
   Size:        946     139     146      29

  Fragments arranged by size: 

                 946     146     139      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781     877     895     970    1260 
   Size:        781      96      18      75     290

  Fragments arranged by size: 

                 781     290      96      75      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     574     977    1260 
   Size:        574     403     283

  Fragments arranged by size: 

                 574     403     283
 
EcoRV GAT'ATC

Cuts at:      0     835     918    1260 
   Size:        835      83     342

  Fragments arranged by size: 

                 835     342      83
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     132     462    1243    1260 
   Size:        132     330     781      17

  Fragments arranged by size: 

                 781     330     132      17
 
HindIII A'AGCT_T

Cuts at:      0     771    1050    1260 
   Size:        771     279     210

  Fragments arranged by size: 

                 771     279     210
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1066    1260 
   Size:       1066     194
 
MscI TGG'CCA

Cuts at:      0     412    1260 
   Size:        412     848
 
MslI CAynn'nnrTG

Cuts at:      0     331     406     611     991    1260 
   Size:        331      75     205     380     269

  Fragments arranged by size: 

                 380     331     269     205      75
 
NdeI CA'TA_TG

Cuts at:      0     379    1260 
   Size:        379     881
 
PshAI GACnn'nnGTC

Cuts at:      0     380    1260 
   Size:        380     880
 
PsiI TTA'TAA

Cuts at:      0    1148    1260 
   Size:       1148     112
 
PstI C_TGCA'G

Cuts at:      0     375     540    1260 
   Size:        375     165     720

  Fragments arranged by size: 

                 720     375     165
 
SapI GCTCTTCn'nnn_

Cuts at:      0     946    1260 
   Size:        946     314
 
ScaI AGT'ACT

Cuts at:      0      76     898    1260 
   Size:         76     822     362

  Fragments arranged by size: 

                 822     362      76
 
SfcI C'TryA_G

Cuts at:      0     371     536    1260 
   Size:        371     165     724

  Fragments arranged by size: 

                 724     371     165
 
SmlI C'TyrA_G

Cuts at:      0    1231    1260 
   Size:       1231      29
 
StyI C'CwwG_G

Cuts at:      0     680    1260 
   Size:        680     580
 
TatI w'GTAC_w

Cuts at:      0      74     896    1260 
   Size:         74     822     364

  Fragments arranged by size: 

                 822     364      74
 
XmnI GAAnn'nnTTC

Cuts at:      0     620     749    1190    1260 
   Size:        620     129     441      70

  Fragments arranged by size: 

                 620     441     129      70
 

 Enzymes that do cut:

     AcuI    AflII     AleI     AlfI     ApoI     AvaI    BanII     BbsI
     BpmI   Bpu10I    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BspHI     BtgI    CspCI     EaeI     EarI  Eco57MI    EcoNI
    EcoRV     FalI  HindIII     MmeI     MscI     MslI     NdeI    PshAI
     PsiI     PstI     SapI     ScaI     SfcI     SmlI     StyI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI EcoO109I    EcoRI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI