Restriction map for
pF1KB0953
(Linear) (Six-base) MAPSORT of: KIBB0953.seq Check: 5683 from: 1 to: 552
KIBB0953.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 118 552
Size: 40 78 434
Fragments arranged by size:
434 78 40
AlwNI CAG_nnn'CTG
Cuts at: 0 324 384 552
Size: 324 60 168
Fragments arranged by size:
324 168 60
BanII G_rGCy'C
Cuts at: 0 8 317 552
Size: 8 309 235
Fragments arranged by size:
309 235 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 373 450 552
Size: 373 77 102
Fragments arranged by size:
373 102 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 301 410 445 552
Size: 184 117 109 35 107
Fragments arranged by size:
184 117 109 107 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 552
Size: 115 30 407
Fragments arranged by size:
407 115 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 552
Size: 349 203
BseYI C'CCAG_C
Cuts at: 0 141 552
Size: 141 411
BsiHKAI G_wGCw'C
Cuts at: 0 170 250 317 396 552
Size: 170 80 67 79 156
Fragments arranged by size:
170 156 80 79 67
Bsp1286I G_dGCh'C
Cuts at: 0 8 170 250 317 396 552
Size: 8 162 80 67 79 156
Fragments arranged by size:
162 156 80 79 67 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 500 552
Size: 392 108 52
Fragments arranged by size:
392 108 52
BsrBI CCG'CTC
Cuts at: 0 42 552
Size: 42 510
BstAPI GCAn_nnn'nTGC
Cuts at: 0 384 552
Size: 384 168
EaeI y'GGCC_r
Cuts at: 0 204 344 552
Size: 204 140 208
Fragments arranged by size:
208 204 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 246 552
Size: 246 306
EcoICRI GAG'CTC
Cuts at: 0 315 552
Size: 315 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 118 184 301 410 445 552
Size: 40 78 66 117 109 35 107
Fragments arranged by size:
117 109 107 78 66 40 35
EcoO109I rG'GnC_Cy
Cuts at: 0 381 420 552
Size: 381 39 132
Fragments arranged by size:
381 132 39
HaeII r_GCGC'y
Cuts at: 0 342 476 552
Size: 342 134 76
Fragments arranged by size:
342 134 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 552
Size: 115 32 405
Fragments arranged by size:
405 115 32
MscI TGG'CCA
Cuts at: 0 206 552
Size: 206 346
MspA1I CmG'CkG
Cuts at: 0 309 547 552
Size: 309 238 5
Fragments arranged by size:
309 238 5
NspI r_CATG'y
Cuts at: 0 297 552
Size: 297 255
PfoI T'CCnGG_A
Cuts at: 0 422 515 552
Size: 422 93 37
Fragments arranged by size:
422 93 37
PpuMI rG'GwC_Cy
Cuts at: 0 381 420 552
Size: 381 39 132
Fragments arranged by size:
381 132 39
PstI C_TGCA'G
Cuts at: 0 472 552
Size: 472 80
PvuII CAG'CTG
Cuts at: 0 547 552
Size: 547 5
SacI G_AGCT'C
Cuts at: 0 317 552
Size: 317 235
SfcI C'TryA_G
Cuts at: 0 468 552
Size: 468 84
SphI G_CATG'C
Cuts at: 0 297 552
Size: 297 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 190 552
Size: 190 362
XmnI GAAnn'nnTTC
Cuts at: 0 103 552
Size: 103 449
Enzymes that do cut:
AcuI AlwNI BanII BplI BpmI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrBI BstAPI EaeI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I MscI MspA1I NspI PfoI
PpuMI PstI PvuII SacI SfcI SphI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI