Restriction map for
pF1KB0950
(Linear) (Six-base) MAPSORT of: KIBB0950.seq Check: 6808 from: 1 to: 1221
KIBB0950.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AfeI AGC'GCT
Cuts at: 0 293 1221
Size: 293 928
AgeI A'CCGG_T
Cuts at: 0 1034 1221
Size: 1034 187
AvaI C'yCGr_G
Cuts at: 0 520 874 1221
Size: 520 354 347
Fragments arranged by size:
520 354 347
BanI G'GyrC_C
Cuts at: 0 308 650 760 1221
Size: 308 342 110 461
Fragments arranged by size:
461 342 308 110
BanII G_rGCy'C
Cuts at: 0 71 875 1221
Size: 71 804 346
Fragments arranged by size:
804 346 71
BbeI G_GCGC'C
Cuts at: 0 654 1221
Size: 654 567
BbsI GAAGACnn'nnnn_
Cuts at: 0 50 1221
Size: 50 1171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 855 889 1221
Size: 855 34 332
Fragments arranged by size:
855 332 34
BglI GCCn_nnn'nGGC
Cuts at: 0 769 850 1221
Size: 769 81 371
Fragments arranged by size:
769 371 81
BglII A'GATC_T
Cuts at: 0 983 1221
Size: 983 238
Bme1580I G_kGCm'C
Cuts at: 0 551 765 1221
Size: 551 214 456
Fragments arranged by size:
551 456 214
BmrI ACTGGGnnnn_n'
Cuts at: 0 613 960 1221
Size: 613 347 261
Fragments arranged by size:
613 347 261
BmtI G_CTAG'C
Cuts at: 0 1168 1221
Size: 1168 53
Bpu10I CC'TnA_GC
Cuts at: 0 80 1221
Size: 80 1141
BsaAI yAC'GTr
Cuts at: 0 882 1221
Size: 882 339
BsaHI Gr'CG_yC
Cuts at: 0 651 782 1221
Size: 651 131 439
Fragments arranged by size:
651 439 131
BsaWI w'CCGG_w
Cuts at: 0 1034 1221
Size: 1034 187
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 1221
Size: 514 707
BseYI C'CCAG_C
Cuts at: 0 391 487 810 1221
Size: 391 96 323 411
Fragments arranged by size:
411 391 323 96
BsiEI CG_ry'CG
Cuts at: 0 268 612 1221
Size: 268 344 609
Fragments arranged by size:
609 344 268
BsmBI CGTCTCn'nnnn_
Cuts at: 0 653 1221
Size: 653 568
Bsp1286I G_dGCh'C
Cuts at: 0 71 551 765 875 1221
Size: 71 480 214 110 346
Fragments arranged by size:
480 346 214 110 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 1221
Size: 125 1096
BsrBI CCG'CTC
Cuts at: 0 435 1137 1221
Size: 435 702 84
Fragments arranged by size:
702 435 84
BsrFI r'CCGG_y
Cuts at: 0 998 1034 1058 1221
Size: 998 36 24 163
Fragments arranged by size:
998 163 36 24
BssSI C'ACGA_G
Cuts at: 0 1072 1221
Size: 1072 149
BstAPI GCAn_nnn'nTGC
Cuts at: 0 452 1221
Size: 452 769
BstEII G'GTnAC_C
Cuts at: 0 297 1221
Size: 297 924
BstXI CCAn_nnnn'nTGG
Cuts at: 0 846 910 1221
Size: 846 64 311
Fragments arranged by size:
846 311 64
BstYI r'GATC_y
Cuts at: 0 983 1221
Size: 983 238
Bsu36I CC'TnA_GG
Cuts at: 0 341 992 1221
Size: 341 651 229
Fragments arranged by size:
651 341 229
BtgI C'CryG_G
Cuts at: 0 705 845 903 1004 1221
Size: 705 140 58 101 217
Fragments arranged by size:
705 217 140 101 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 923 1221
Size: 923 298
BtsI GCAGTG_nn'
Cuts at: 0 212 471 477 795 1221
Size: 212 259 6 318 426
Fragments arranged by size:
426 318 259 212 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 627 662 1221
Size: 627 35 559
Fragments arranged by size:
627 559 35
DraIII CAC_nnn'GTG
Cuts at: 0 1077 1221
Size: 1077 144
EaeI y'GGCC_r
Cuts at: 0 87 265 620 1221
Size: 87 178 355 601
Fragments arranged by size:
601 355 178 87
EagI C'GGCC_G
Cuts at: 0 265 1221
Size: 265 956
EarI CTCTTCn'nnn_
Cuts at: 0 230 1221
Size: 230 991
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 781 1077 1221
Size: 781 296 144
Fragments arranged by size:
781 296 144
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 408 1221
Size: 31 377 813
Fragments arranged by size:
813 377 31
EcoO109I rG'GnC_Cy
Cuts at: 0 185 345 633 1221
Size: 185 160 288 588
Fragments arranged by size:
588 288 185 160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 603 1221
Size: 603 618
FseI GG_CCGG'CC
Cuts at: 0 1002 1221
Size: 1002 219
FspI TGC'GCA
Cuts at: 0 801 1221
Size: 801 420
HaeII r_GCGC'y
Cuts at: 0 287 295 508 528 654 918 1221
Size: 287 8 213 20 126 264 303
Fragments arranged by size:
303 287 264 213 126 20 8
HincII GTy'rAC
Cuts at: 0 894 1221
Size: 894 327
KasI G'GCGC_C
Cuts at: 0 650 1221
Size: 650 571
MslI CAynn'nnrTG
Cuts at: 0 908 1221
Size: 908 313
MspA1I CmG'CkG
Cuts at: 0 270 458 1221
Size: 270 188 763
Fragments arranged by size:
763 270 188
NaeI GCC'GGC
Cuts at: 0 1000 1221
Size: 1000 221
NarI GG'CG_CC
Cuts at: 0 651 1221
Size: 651 570
NcoI C'CATG_G
Cuts at: 0 845 1221
Size: 845 376
NgoMIV G'CCGG_C
Cuts at: 0 998 1221
Size: 998 223
NheI G'CTAG_C
Cuts at: 0 1164 1221
Size: 1164 57
NotI GC'GGCC_GC
Cuts at: 0 265 1221
Size: 265 956
NspI r_CATG'y
Cuts at: 0 558 1221
Size: 558 663
PasI CC'CwG_GG
Cuts at: 0 327 404 1221
Size: 327 77 817
Fragments arranged by size:
817 327 77
PflMI CCAn_nnn'nTGG
Cuts at: 0 318 1221
Size: 318 903
PfoI T'CCnGG_A
Cuts at: 0 150 1221
Size: 150 1071
PpuMI rG'GwC_Cy
Cuts at: 0 185 1221
Size: 185 1036
PstI C_TGCA'G
Cuts at: 0 275 291 457 463 983 1221
Size: 275 16 166 6 520 238
Fragments arranged by size:
520 275 238 166 16 6
PvuII CAG'CTG
Cuts at: 0 458 1221
Size: 458 763
SanDI GG'GwC_CC
Cuts at: 0 185 1221
Size: 185 1036
SexAI A'CCwGG_T
Cuts at: 0 1096 1221
Size: 1096 125
SfcI C'TryA_G
Cuts at: 0 271 287 453 459 979 1221
Size: 271 16 166 6 520 242
Fragments arranged by size:
520 271 242 166 16 6
SfoI GGC'GCC
Cuts at: 0 652 1221
Size: 652 569
SnaBI TAC'GTA
Cuts at: 0 882 1221
Size: 882 339
SphI G_CATG'C
Cuts at: 0 558 1221
Size: 558 663
StuI AGG'CCT
Cuts at: 0 169 1221
Size: 169 1052
StyI C'CwwG_G
Cuts at: 0 71 302 839 845 1130 1221
Size: 71 231 537 6 285 91
Fragments arranged by size:
537 285 231 91 71 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 405 714 1221
Size: 405 309 507
Fragments arranged by size:
507 405 309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 1221
Size: 27 1194
Tth111I GACn'n_nGTC
Cuts at: 0 784 1221
Size: 784 437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 846 1221
Size: 102 744 375
Fragments arranged by size:
744 375 102
Enzymes that do cut:
AfeI AgeI AvaI BanI BanII BbeI BbsI BcgI
BglI BglII Bme1580I BmrI BmtI Bpu10I BsaAI BsaHI
BsaWI BseRI BseYI BsiEI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI
EcoNI EcoO109I FalI FseI FspI HaeII HincII KasI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NspI PasI PflMI PfoI PpuMI PstI PvuII SanDI
SexAI SfcI SfoI SnaBI SphI StuI StyI TaqII
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BlpI BmgBI BplI BpmI BpuEI BsaI
BsaBI BsaXI BsgI BsiHKAI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssHII BstBI BstZ17I ClaI DraI DrdI
EcoICRI Eco57MI EcoRI EcoRV FspAI Hin4I HindIII HpaI
KpnI MfeI MluI MmeI MscI NdeI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SgfI SgrAI SmaI SmlI SpeI
SrfI SspI SwaI TatI XbaI XhoI XmaI XmnI
ZraI