(Linear) (Six-base) MAPSORT of: KIBB0949.seq  Check: 3870  from: 1  to: 540



KIBB0949.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     540 
   Size:        175     365
 
BanI G'GyrC_C

Cuts at:      0       3      55     540 
   Size:          3      52     485

  Fragments arranged by size: 

                 485      52       3
 
BanII G_rGCy'C

Cuts at:      0     253     540 
   Size:        253     287
 
BbeI G_GCGC'C

Cuts at:      0       7      59     540 
   Size:          7      52     481

  Fragments arranged by size: 

                 481      52       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     469     540 
   Size:        469      71
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     425     540 
   Size:        425     115
 
BclI T'GATC_A

Cuts at:      0     293     540 
   Size:        293     247
 
BglI GCCn_nnn'nGGC

Cuts at:      0      53     540 
   Size:         53     487
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     202     540 
   Size:        202     338
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     540 
   Size:         57     483
 
BsaHI Gr'CG_yC

Cuts at:      0       4      28      56     540 
   Size:          4      24      28     484

  Fragments arranged by size: 

                 484      28      24       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     316     387     412     436     540 
   Size:        316      71      25      24     104

  Fragments arranged by size: 

                 316     104      71      25      24
 
BseYI C'CCAG_C

Cuts at:      0     260     540 
   Size:        260     280
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     540 
   Size:        239     301
 
BsiHKAI G_wGCw'C

Cuts at:      0     253     540 
   Size:        253     287
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     168     540 
   Size:        168     372
 
Bsp1286I G_dGCh'C

Cuts at:      0     253     540 
   Size:        253     287
 
BsrBI CCG'CTC

Cuts at:      0     417     540 
   Size:        417     123
 
BsrDI GCAATG_nn'

Cuts at:      0     335     540 
   Size:        335     205
 
BsrFI r'CCGG_y

Cuts at:      0     228     540 
   Size:        228     312
 
BtgI C'CryG_G

Cuts at:      0      18     540 
   Size:         18     522
 
DraI TTT'AAA

Cuts at:      0     504     540 
   Size:        504      36
 
EaeI y'GGCC_r

Cuts at:      0      86     540 
   Size:         86     454
 
EarI CTCTTCn'nnn_

Cuts at:      0     140     364     540 
   Size:        140     224     176

  Fragments arranged by size: 

                 224     176     140
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     444     540 
   Size:        444      96
 
EcoICRI GAG'CTC

Cuts at:      0     251     540 
   Size:        251     289
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     175     540 
   Size:         57     118     365

  Fragments arranged by size: 

                 365     118      57
 
HaeII r_GCGC'y

Cuts at:      0       7      59     292     540 
   Size:          7      52     233     248

  Fragments arranged by size: 

                 248     233      52       7
 
KasI G'GCGC_C

Cuts at:      0       3      55     540 
   Size:          3      52     485

  Fragments arranged by size: 

                 485      52       3
 
MslI CAynn'nnrTG

Cuts at:      0      82     301     540 
   Size:         82     219     239

  Fragments arranged by size: 

                 239     219      82
 
MspA1I CmG'CkG

Cuts at:      0      20     540 
   Size:         20     520
 
NarI GG'CG_CC

Cuts at:      0       4      56     540 
   Size:          4      52     484

  Fragments arranged by size: 

                 484      52       4
 
PfoI T'CCnGG_A

Cuts at:      0     105     540 
   Size:        105     435
 
PstI C_TGCA'G

Cuts at:      0     364     540 
   Size:        364     176
 
SacI G_AGCT'C

Cuts at:      0     253     540 
   Size:        253     287
 
SacII CC_GC'GG

Cuts at:      0      21     540 
   Size:         21     519
 
SfcI C'TryA_G

Cuts at:      0     360     540 
   Size:        360     180
 
SfoI GGC'GCC

Cuts at:      0       5      57     540 
   Size:          5      52     483

  Fragments arranged by size: 

                 483      52       5
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     264     540 
   Size:        264     276
 

 Enzymes that do cut:

     AcuI     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI
     BmrI     BpmI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BsrBI    BsrDI    BsrFI     BtgI     DraI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    HaeII     KasI     MslI   MspA1I     NarI
     PfoI     PstI     SacI    SacII     SfcI     SfoI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI