(Linear) (Six-base) MAPSORT of: KIBB0936.seq  Check: 3221  from: 1  to: 1158



KIBB0936.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     788    1158 
   Size:        211     577     370

  Fragments arranged by size: 

                 577     370     211
 
AleI CACnn'nnGTG

Cuts at:      0     769     897    1158 
   Size:        769     128     261

  Fragments arranged by size: 

                 769     261     128
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     582     614    1158 
   Size:        582      32     544

  Fragments arranged by size: 

                 582     544      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     234     735    1021    1158 
   Size:        234     501     286     137

  Fragments arranged by size: 

                 501     286     234     137
 
ApoI r'AATT_y

Cuts at:      0     320     907    1158 
   Size:        320     587     251

  Fragments arranged by size: 

                 587     320     251
 
BanII G_rGCy'C

Cuts at:      0     410     565    1158 
   Size:        410     155     593

  Fragments arranged by size: 

                 593     410     155
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     559     662    1009    1158 
   Size:        559     103     347     149

  Fragments arranged by size: 

                 559     347     149     103
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     204     398    1158 
   Size:        204     194     760

  Fragments arranged by size: 

                 760     204     194
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     979    1158 
   Size:        979     179
 
Bpu10I CC'TnA_GC

Cuts at:      0    1076    1158 
   Size:       1076      82
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579    1116    1158 
   Size:        579     537      42

  Fragments arranged by size: 

                 579     537      42
 
BsaWI w'CCGG_w

Cuts at:      0      37    1158 
   Size:         37    1121
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       7      37    1158 
   Size:          7      30    1121

  Fragments arranged by size: 

                1121      30       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     320     824    1158 
   Size:        320     504     334

  Fragments arranged by size: 

                 504     334     320
 
BsiHKAI G_wGCw'C

Cuts at:      0     410    1158 
   Size:        410     748
 
BsmI GAATG_Cn'

Cuts at:      0     878    1158 
   Size:        878     280
 
Bsp1286I G_dGCh'C

Cuts at:      0     410     565    1158 
   Size:        410     155     593

  Fragments arranged by size: 

                 593     410     155
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     720    1158 
   Size:        720     438
 
BsrDI GCAATG_nn'

Cuts at:      0     238    1158 
   Size:        238     920
 
BsrFI r'CCGG_y

Cuts at:      0     387    1158 
   Size:        387     771
 
BsrGI T'GTAC_A

Cuts at:      0    1031    1158 
   Size:       1031     127
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     138    1158 
   Size:        138    1020
 
BtgI C'CryG_G

Cuts at:      0     935    1158 
   Size:        935     223
 
BtsI GCAGTG_nn'

Cuts at:      0     278    1158 
   Size:        278     880
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      41      76    1158 
   Size:         41      35    1082

  Fragments arranged by size: 

                1082      41      35
 
DraI TTT'AAA

Cuts at:      0     113    1158 
   Size:        113    1045
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      91    1158 
   Size:         91    1067
 
EarI CTCTTCn'nnn_

Cuts at:      0     230     398    1119    1124    1158 
   Size:        230     168     721       5      34

  Fragments arranged by size: 

                 721     230     168      34       5
 
EcoICRI GAG'CTC

Cuts at:      0     408    1158 
   Size:        408     750
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     788     979    1158 
   Size:        211     577     191     179

  Fragments arranged by size: 

                 577     211     191     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0     453     571     732    1158 
   Size:        453     118     161     426

  Fragments arranged by size: 

                 453     426     161     118
 
EcoRV GAT'ATC

Cuts at:      0    1113    1158 
   Size:       1113      45
 
MfeI C'AATT_G

Cuts at:      0    1145    1158 
   Size:       1145      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1081    1158 
   Size:       1081      77
 
MslI CAynn'nnrTG

Cuts at:      0     769     897    1158 
   Size:        769     128     261

  Fragments arranged by size: 

                 769     261     128
 
NcoI C'CATG_G

Cuts at:      0     935    1158 
   Size:        935     223
 
NdeI CA'TA_TG

Cuts at:      0     167    1158 
   Size:        167     991
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     844    1158 
   Size:        844     314
 
PfoI T'CCnGG_A

Cuts at:      0     858    1158 
   Size:        858     300
 
PpuMI rG'GwC_Cy

Cuts at:      0     453     571     732    1158 
   Size:        453     118     161     426

  Fragments arranged by size: 

                 453     426     161     118
 
SacI G_AGCT'C

Cuts at:      0     410    1158 
   Size:        410     748
 
SanDI GG'GwC_CC

Cuts at:      0     453    1158 
   Size:        453     705
 
SapI GCTCTTCn'nnn_

Cuts at:      0     398    1158 
   Size:        398     760
 
SmlI C'TyrA_G

Cuts at:      0     558    1095    1158 
   Size:        558     537      63

  Fragments arranged by size: 

                 558     537      63
 
StyI C'CwwG_G

Cuts at:      0     935    1158 
   Size:        935     223
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     710    1158 
   Size:        710     448
 
TatI w'GTAC_w

Cuts at:      0     552    1031    1158 
   Size:        552     479     127

  Fragments arranged by size: 

                 552     479     127
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     608    1158 
   Size:        608     550
 

 Enzymes that do cut:

     AcuI     AleI     AloI    AlwNI     ApoI    BanII     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaWI    BsaXI    BseRI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI    BstXI     BtgI     BtsI
    CspCI     DraI     DrdI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV
     MfeI     MmeI     MslI     NcoI     NdeI    PflMI     PfoI    PpuMI
     SacI    SanDI     SapI     SmlI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
   DraIII     EaeI     EagI     EciI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MscI   MspA1I     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI