(Linear) (Six-base) MAPSORT of: KIBB0933.seq  Check: 9976  from: 1  to: 444



KIBB0933.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12     444 
   Size:         12     432
 
AlwNI CAG_nnn'CTG

Cuts at:      0      57     444 
   Size:         57     387
 
ApaI G_GGCC'C

Cuts at:      0     419     444 
   Size:        419      25
 
ApoI r'AATT_y

Cuts at:      0     166     216     444 
   Size:        166      50     228

  Fragments arranged by size: 

                 228     166      50
 
AvaI C'yCGr_G

Cuts at:      0     260     444 
   Size:        260     184
 
BanII G_rGCy'C

Cuts at:      0       7     230     419     444 
   Size:          7     223     189      25

  Fragments arranged by size: 

                 223     189      25       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      96     254     444 
   Size:         96     158     190

  Fragments arranged by size: 

                 190     158      96
 
Bme1580I G_kGCm'C

Cuts at:      0     419     444 
   Size:        419      25
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     372     444 
   Size:        372      72
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     444 
   Size:        346      98
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     444 
   Size:        254     190
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     230     444 
   Size:          7     223     214

  Fragments arranged by size: 

                 223     214       7
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     230     419     444 
   Size:          7     223     189      25

  Fragments arranged by size: 

                 223     189      25       7
 
Bsu36I CC'TnA_GG

Cuts at:      0     410     444 
   Size:        410      34
 
BtgI C'CryG_G

Cuts at:      0     399     444 
   Size:        399      45
 
BtsI GCAGTG_nn'

Cuts at:      0     242     444 
   Size:        242     202
 
EarI CTCTTCn'nnn_

Cuts at:      0     127     444 
   Size:        127     317
 
EcoICRI GAG'CTC

Cuts at:      0       5     228     444 
   Size:          5     223     216

  Fragments arranged by size: 

                 223     216       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12     346     444 
   Size:         12     334      98

  Fragments arranged by size: 

                 334      98      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     415     416     444 
   Size:        415       1      28

  Fragments arranged by size: 

                 415      28       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     312     444 
   Size:        312     132
 
MspA1I CmG'CkG

Cuts at:      0     372     444 
   Size:        372      72
 
NspI r_CATG'y

Cuts at:      0     360     444 
   Size:        360      84
 
PspOMI G'GGCC_C

Cuts at:      0     415     444 
   Size:        415      29
 
PvuII CAG'CTG

Cuts at:      0     372     444 
   Size:        372      72
 
SacI G_AGCT'C

Cuts at:      0       7     230     444 
   Size:          7     223     214

  Fragments arranged by size: 

                 223     214       7
 
SfcI C'TryA_G

Cuts at:      0     367     444 
   Size:        367      77
 
SmlI C'TyrA_G

Cuts at:      0     260     444 
   Size:        260     184
 
SspI AAT'ATT

Cuts at:      0      42     444 
   Size:         42     402
 
XhoI C'TCGA_G

Cuts at:      0     260     444 
   Size:        260     184
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     ApoI     AvaI    BanII     BbsI Bme1580I
     BmrI     BpmI     BsgI  BsiHKAI Bsp1286I   Bsu36I     BtgI     BtsI
     EarI  EcoICRI  Eco57MI EcoO109I     FalI   MspA1I     NspI   PspOMI
    PvuII     SacI     SfcI     SmlI     SspI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI