(Linear) (Six-base) MAPSORT of: KIBB0931.seq  Check: 4896  from: 1  to: 435



KIBB0931.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AccI GT'mk_AC

Cuts at:      0     245     435 
   Size:        245     190
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     254     435 
   Size:        254     181
 
ApaI G_GGCC'C

Cuts at:      0      59     435 
   Size:         59     376
 
BanI G'GyrC_C

Cuts at:      0     372     435 
   Size:        372      63
 
BanII G_rGCy'C

Cuts at:      0      59     435 
   Size:         59     376
 
BglI GCCn_nnn'nGGC

Cuts at:      0      63     435 
   Size:         63     372
 
Bme1580I G_kGCm'C

Cuts at:      0      59     435 
   Size:         59     376
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     435 
   Size:        160     275
 
BseYI C'CCAG_C

Cuts at:      0      66     435 
   Size:         66     369
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     307     325     435 
   Size:        214      93      18     110

  Fragments arranged by size: 

                 214     110      93      18
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     435 
   Size:         59     376
 
BspHI T'CATG_A

Cuts at:      0     413     435 
   Size:        413      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     233     435 
   Size:        233     202
 
BsrFI r'CCGG_y

Cuts at:      0     422     435 
   Size:        422      13
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     268     435 
   Size:        268     167
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     363     435 
   Size:        363      72
 
BstYI r'GATC_y

Cuts at:      0     191     435 
   Size:        191     244
 
Bsu36I CC'TnA_GG

Cuts at:      0      60     435 
   Size:         60     375
 
BtgI C'CryG_G

Cuts at:      0     392     435 
   Size:        392      43
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      99     134     435 
   Size:         99      35     301

  Fragments arranged by size: 

                 301      99      35
 
EaeI y'GGCC_r

Cuts at:      0     207     435 
   Size:        207     228
 
EarI CTCTTCn'nnn_

Cuts at:      0       3      29     435 
   Size:          3      26     406

  Fragments arranged by size: 

                 406      26       3
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     435 
   Size:        160     275
 
EcoO109I rG'GnC_Cy

Cuts at:      0      55      56     339     435 
   Size:         55       1     283      96

  Fragments arranged by size: 

                 283      96      55       1
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     435 
   Size:        134     301
 
MscI TGG'CCA

Cuts at:      0     209     435 
   Size:        209     226
 
MslI CAynn'nnrTG

Cuts at:      0     236     329     361     435 
   Size:        236      93      32      74

  Fragments arranged by size: 

                 236      93      74      32
 
NcoI C'CATG_G

Cuts at:      0     392     435 
   Size:        392      43
 
PfoI T'CCnGG_A

Cuts at:      0     176     435 
   Size:        176     259
 
PpuMI rG'GwC_Cy

Cuts at:      0     339     435 
   Size:        339      96
 
PspOMI G'GGCC_C

Cuts at:      0      55     435 
   Size:         55     380
 
SanDI GG'GwC_CC

Cuts at:      0     339     435 
   Size:        339      96
 
SfcI C'TryA_G

Cuts at:      0     242     317     435 
   Size:        242      75     118

  Fragments arranged by size: 

                 242     118      75
 
StyI C'CwwG_G

Cuts at:      0     392     435 
   Size:        392      43
 

 Enzymes that do cut:

     AccI     AlfI     ApaI     BanI    BanII     BglI Bme1580I     BpmI
    BseYI     BsgI Bsp1286I    BspHI    BspMI    BsrFI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI    CspCI     EaeI     EarI  Eco57MI EcoO109I
     MmeI     MscI     MslI     NcoI     PfoI    PpuMI   PspOMI    SanDI
     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI