Restriction map for
pF1KB0927
(Linear) (Six-base) MAPSORT of: KIBB0927.seq Check: 6727 from: 1 to: 399
KIBB0927.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AflIII A'CryG_T
Cuts at: 0 339 399
Size: 339 60
BanI G'GyrC_C
Cuts at: 0 103 399
Size: 103 296
BanII G_rGCy'C
Cuts at: 0 233 366 399
Size: 233 133 33
Fragments arranged by size:
233 133 33
BbeI G_GCGC'C
Cuts at: 0 107 399
Size: 107 292
BglII A'GATC_T
Cuts at: 0 29 399
Size: 29 370
BmrI ACTGGGnnnn_n'
Cuts at: 0 322 399
Size: 322 77
BmtI G_CTAG'C
Cuts at: 0 383 399
Size: 383 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 311 399
Size: 97 214 88
Fragments arranged by size:
214 97 88
Bpu10I CC'TnA_GC
Cuts at: 0 186 399
Size: 186 213
BsaI GGTCTCn'nnnn_
Cuts at: 0 212 266 399
Size: 212 54 133
Fragments arranged by size:
212 133 54
BsaHI Gr'CG_yC
Cuts at: 0 104 199 399
Size: 104 95 200
Fragments arranged by size:
200 104 95
BsaWI w'CCGG_w
Cuts at: 0 239 399
Size: 239 160
BseYI C'CCAG_C
Cuts at: 0 211 333 399
Size: 211 122 66
Fragments arranged by size:
211 122 66
BsiHKAI G_wGCw'C
Cuts at: 0 233 399
Size: 233 166
Bsp1286I G_dGCh'C
Cuts at: 0 233 366 399
Size: 233 133 33
Fragments arranged by size:
233 133 33
BspEI T'CCGG_A
Cuts at: 0 239 399
Size: 239 160
BsrFI r'CCGG_y
Cuts at: 0 100 399
Size: 100 299
BstYI r'GATC_y
Cuts at: 0 29 399
Size: 29 370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 71 399
Size: 71 328
EaeI y'GGCC_r
Cuts at: 0 98 176 307 369 399
Size: 98 78 131 62 30
Fragments arranged by size:
131 98 78 62 30
EarI CTCTTCn'nnn_
Cuts at: 0 290 399
Size: 290 109
EcoICRI GAG'CTC
Cuts at: 0 231 399
Size: 231 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 311 399
Size: 97 214 88
Fragments arranged by size:
214 97 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 399
Size: 115 284
HaeII r_GCGC'y
Cuts at: 0 107 399
Size: 107 292
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 146 178 234 266 399
Size: 146 32 56 32 133
Fragments arranged by size:
146 133 56 32 32
HindIII A'AGCT_T
Cuts at: 0 183 399
Size: 183 216
KasI G'GCGC_C
Cuts at: 0 103 399
Size: 103 296
MspA1I CmG'CkG
Cuts at: 0 215 399
Size: 215 184
NaeI GCC'GGC
Cuts at: 0 102 399
Size: 102 297
NarI GG'CG_CC
Cuts at: 0 104 399
Size: 104 295
NgoMIV G'CCGG_C
Cuts at: 0 100 399
Size: 100 299
NheI G'CTAG_C
Cuts at: 0 379 399
Size: 379 20
NspI r_CATG'y
Cuts at: 0 343 399
Size: 343 56
PasI CC'CwG_GG
Cuts at: 0 193 399
Size: 193 206
PciI A'CATG_T
Cuts at: 0 339 399
Size: 339 60
PfoI T'CCnGG_A
Cuts at: 0 113 399
Size: 113 286
SacI G_AGCT'C
Cuts at: 0 233 399
Size: 233 166
SapI GCTCTTCn'nnn_
Cuts at: 0 290 399
Size: 290 109
SfoI GGC'GCC
Cuts at: 0 105 399
Size: 105 294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 355 399
Size: 255 100 44
Fragments arranged by size:
255 100 44
Enzymes that do cut:
AflIII BanI BanII BbeI BglII BmrI BmtI BpmI
Bpu10I BsaI BsaHI BsaWI BseYI BsiHKAI Bsp1286I BspEI
BsrFI BstYI BtgZI EaeI EarI EcoICRI Eco57MI EcoNI
HaeII Hin4I HindIII KasI MspA1I NaeI NarI NgoMIV
NheI NspI PasI PciI PfoI SacI SapI SfoI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
Bme1580I BmgBI BplI BpuEI BsaAI BsaBI BsaXI BseRI
BsgI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI MmeI
MscI MslI NcoI NdeI NotI NruI NsiI PacI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI