Restriction map for
pF1KB0926
(Linear) (Six-base) MAPSORT of: KIBB0926.seq Check: 8662 from: 1 to: 1068
KIBB0926.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 350 1068
Size: 350 718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 918 994 1027 1068
Size: 414 504 76 33 41
Fragments arranged by size:
504 414 76 41 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 155 979 1068
Size: 155 824 89
Fragments arranged by size:
824 155 89
AlwNI CAG_nnn'CTG
Cuts at: 0 264 342 455 912 1068
Size: 264 78 113 457 156
Fragments arranged by size:
457 264 156 113 78
ApaI G_GGCC'C
Cuts at: 0 149 197 1068
Size: 149 48 871
Fragments arranged by size:
871 149 48
AvaI C'yCGr_G
Cuts at: 0 196 1068
Size: 196 872
BanI G'GyrC_C
Cuts at: 0 729 1068
Size: 729 339
BanII G_rGCy'C
Cuts at: 0 44 149 197 604 932 1068
Size: 44 105 48 407 328 136
Fragments arranged by size:
407 328 136 105 48 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 261 704 1068
Size: 261 443 364
Fragments arranged by size:
443 364 261
BbvCI CC'TCA_GC
Cuts at: 0 49 739 1068
Size: 49 690 329
Fragments arranged by size:
690 329 49
BglI GCCn_nnn'nGGC
Cuts at: 0 100 201 1068
Size: 100 101 867
Fragments arranged by size:
867 101 100
Bme1580I G_kGCm'C
Cuts at: 0 149 197 1068
Size: 149 48 871
Fragments arranged by size:
871 149 48
BmgBI CAC'GTC
Cuts at: 0 876 1068
Size: 876 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 668 731 738 1068
Size: 175 493 63 7 330
Fragments arranged by size:
493 330 175 63 7
Bpu10I CC'TnA_GC
Cuts at: 0 9 49 739 1068
Size: 9 40 690 329
Fragments arranged by size:
690 329 40 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 1068
Size: 786 282
BsaAI yAC'GTr
Cuts at: 0 924 1068
Size: 924 144
BsaHI Gr'CG_yC
Cuts at: 0 896 1068
Size: 896 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 586 601 616 631 1068
Size: 37 30 519 15 15 15 437
Fragments arranged by size:
519 437 37 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 606 609 1068
Size: 606 3 459
Fragments arranged by size:
606 459 3
BseYI C'CCAG_C
Cuts at: 0 82 1011 1068
Size: 82 929 57
Fragments arranged by size:
929 82 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1068
Size: 190 878
BsiHKAI G_wGCw'C
Cuts at: 0 932 1068
Size: 932 136
Bsp1286I G_dGCh'C
Cuts at: 0 44 149 197 604 932 1068
Size: 44 105 48 407 328 136
Fragments arranged by size:
407 328 136 105 48 44
BspHI T'CATG_A
Cuts at: 0 989 1068
Size: 989 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 1068
Size: 350 718
BsrBI CCG'CTC
Cuts at: 0 201 1068
Size: 201 867
BssSI C'ACGA_G
Cuts at: 0 943 1068
Size: 943 125
BstAPI GCAn_nnn'nTGC
Cuts at: 0 245 1068
Size: 245 823
Bsu36I CC'TnA_GG
Cuts at: 0 62 1068
Size: 62 1006
BtgI C'CryG_G
Cuts at: 0 25 74 838 1068
Size: 25 49 764 230
Fragments arranged by size:
764 230 49 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 434 1068
Size: 434 634
BtsI GCAGTG_nn'
Cuts at: 0 465 1068
Size: 465 603
EaeI y'GGCC_r
Cuts at: 0 202 835 1068
Size: 202 633 233
Fragments arranged by size:
633 233 202
EarI CTCTTCn'nnn_
Cuts at: 0 433 521 937 970 1068
Size: 433 88 416 33 98
Fragments arranged by size:
433 416 98 88 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 567 846 1068
Size: 567 279 222
Fragments arranged by size:
567 279 222
EcoICRI GAG'CTC
Cuts at: 0 930 1068
Size: 930 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 414 668 731 738 918 994 1027
Size: 175 239 254 63 7 180 76 33
Cuts at: 1027 1068
Size: 41
Fragments arranged by size:
254 239 180 175 76 63 41 33
7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 447 1068
Size: 447 621
EcoO109I rG'GnC_Cy
Cuts at: 0 145 146 916 1015 1068
Size: 145 1 770 99 53
Fragments arranged by size:
770 145 99 53 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 285 1068
Size: 285 783
FspI TGC'GCA
Cuts at: 0 821 1068
Size: 821 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 190 222 292 324 1068
Size: 37 32 121 32 70 32 744
Fragments arranged by size:
744 121 70 37 32 32 32
MslI CAynn'nnrTG
Cuts at: 0 988 1068
Size: 988 80
MspA1I CmG'CkG
Cuts at: 0 27 685 1068
Size: 27 658 383
Fragments arranged by size:
658 383 27
PasI CC'CwG_GG
Cuts at: 0 449 1068
Size: 449 619
PfoI T'CCnGG_A
Cuts at: 0 179 476 1068
Size: 179 297 592
Fragments arranged by size:
592 297 179
PmlI CAC'GTG
Cuts at: 0 924 1068
Size: 924 144
PpuMI rG'GwC_Cy
Cuts at: 0 1015 1068
Size: 1015 53
PspOMI G'GGCC_C
Cuts at: 0 145 193 1068
Size: 145 48 875
Fragments arranged by size:
875 145 48
PstI C_TGCA'G
Cuts at: 0 221 460 1068
Size: 221 239 608
Fragments arranged by size:
608 239 221
SacI G_AGCT'C
Cuts at: 0 932 1068
Size: 932 136
SacII CC_GC'GG
Cuts at: 0 28 1068
Size: 28 1040
SanDI GG'GwC_CC
Cuts at: 0 1015 1068
Size: 1015 53
SapI GCTCTTCn'nnn_
Cuts at: 0 937 1068
Size: 937 131
SexAI A'CCwGG_T
Cuts at: 0 533 1068
Size: 533 535
SfcI C'TryA_G
Cuts at: 0 217 456 561 1068
Size: 217 239 105 507
Fragments arranged by size:
507 239 217 105
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 201 1068
Size: 201 867
SmlI C'TyrA_G
Cuts at: 0 801 1068
Size: 801 267
StuI AGG'CCT
Cuts at: 0 744 1068
Size: 744 324
StyI C'CwwG_G
Cuts at: 0 660 1068
Size: 660 408
TatI w'GTAC_w
Cuts at: 0 626 1068
Size: 626 442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 586 601 618 633 865 897 1068
Size: 586 15 17 15 232 32 171
Fragments arranged by size:
586 232 171 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 0 985 1068
Size: 985 83
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 664 1068
Size: 664 404
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI AvaI BanI BanII
BbsI BbvCI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BssSI BstAPI Bsu36I BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI Hin4I MslI MspA1I PasI PfoI PmlI
PpuMI PspOMI PstI SacI SacII SanDI SapI SexAI
SfcI SfiI SmlI StuI StyI TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI BsaI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EcoRI EcoRV FseI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SalI SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII XbaI XhoI
XmaI XmnI ZraI