(Linear) (Six-base) MAPSORT of: KIBB0923.seq  Check: 2907  from: 1  to: 366



KIBB0923.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AflII C'TTAA_G

Cuts at:      0      46     366 
   Size:         46     320
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     366 
   Size:         18     348
 
AvaI C'yCGr_G

Cuts at:      0     290     366 
   Size:        290      76
 
BmtI G_CTAG'C

Cuts at:      0     122     366 
   Size:        122     244
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     366 
   Size:         40     326
 
BsrDI GCAATG_nn'

Cuts at:      0     160     366 
   Size:        160     206
 
BsrFI r'CCGG_y

Cuts at:      0     359     366 
   Size:        359       7
 
BtgI C'CryG_G

Cuts at:      0      92     366 
   Size:         92     274
 
EarI CTCTTCn'nnn_

Cuts at:      0     214     366 
   Size:        214     152
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     288     366 
   Size:        288      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     366 
   Size:         40     326
 
EcoO109I rG'GnC_Cy

Cuts at:      0      88     366 
   Size:         88     278
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      61     284     297     366 
   Size:         61     223      13      69

  Fragments arranged by size: 

                 223      69      61      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      93     125     366 
   Size:         93      32     241

  Fragments arranged by size: 

                 241      93      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     366 
   Size:         79     287
 
NcoI C'CATG_G

Cuts at:      0      92     366 
   Size:         92     274
 
NheI G'CTAG_C

Cuts at:      0     118     366 
   Size:        118     248
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      98     366 
   Size:         98     268
 
PpuMI rG'GwC_Cy

Cuts at:      0      88     366 
   Size:         88     278
 
PspXI vC'TCGA_Gb

Cuts at:      0     290     366 
   Size:        290      76
 
SanDI GG'GwC_CC

Cuts at:      0      88     366 
   Size:         88     278
 
SapI GCTCTTCn'nnn_

Cuts at:      0     214     366 
   Size:        214     152
 
SmlI C'TyrA_G

Cuts at:      0      46     290     366 
   Size:         46     244      76

  Fragments arranged by size: 

                 244      76      46
 
StyI C'CwwG_G

Cuts at:      0      92     366 
   Size:         92     274
 
XhoI C'TCGA_G

Cuts at:      0     290     366 
   Size:        290      76
 

 Enzymes that do cut:

    AflII    AlwNI     AvaI     BmtI     BpmI    BsrDI    BsrFI     BtgI
     EarI     EciI  Eco57MI EcoO109I     FalI    Hin4I     MmeI     NcoI
     NheI    PflMI    PpuMI    PspXI    SanDI     SapI     SmlI     StyI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI     ZraI