Restriction map for
pF1KB0919
(Linear) (Six-base) MAPSORT of: KIBB0919.seq Check: 1088 from: 1 to: 354
KIBB0919.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 354
Size: 129 225
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 91 123 354
Size: 91 32 231
Fragments arranged by size:
231 91 32
AlwNI CAG_nnn'CTG
Cuts at: 0 69 225 354
Size: 69 156 129
Fragments arranged by size:
156 129 69
AvaI C'yCGr_G
Cuts at: 0 171 354
Size: 171 183
BamHI G'GATC_C
Cuts at: 0 99 354
Size: 99 255
BanII G_rGCy'C
Cuts at: 0 7 266 354
Size: 7 259 88
Fragments arranged by size:
259 88 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 354
Size: 134 220
BbvCI CC'TCA_GC
Cuts at: 0 247 354
Size: 247 107
BglI GCCn_nnn'nGGC
Cuts at: 0 208 354
Size: 208 146
BmrI ACTGGGnnnn_n'
Cuts at: 0 130 354
Size: 130 224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 199 354
Size: 199 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 310 354
Size: 91 219 44
Fragments arranged by size:
219 91 44
Bpu10I CC'TnA_GC
Cuts at: 0 247 354
Size: 247 107
BsaAI yAC'GTr
Cuts at: 0 207 354
Size: 207 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 336 354
Size: 336 18
BseYI C'CCAG_C
Cuts at: 0 42 354
Size: 42 312
BsiHKAI G_wGCw'C
Cuts at: 0 266 354
Size: 266 88
Bsp1286I G_dGCh'C
Cuts at: 0 7 266 354
Size: 7 259 88
Fragments arranged by size:
259 88 7
BsrBI CCG'CTC
Cuts at: 0 294 354
Size: 294 60
BsrFI r'CCGG_y
Cuts at: 0 90 277 327 354
Size: 90 187 50 27
Fragments arranged by size:
187 90 50 27
BstYI r'GATC_y
Cuts at: 0 65 99 354
Size: 65 34 255
Fragments arranged by size:
255 65 34
Bsu36I CC'TnA_GG
Cuts at: 0 348 354
Size: 348 6
BtgI C'CryG_G
Cuts at: 0 53 354
Size: 53 301
EcoICRI GAG'CTC
Cuts at: 0 264 354
Size: 264 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 129 310 354
Size: 91 38 181 44
Fragments arranged by size:
181 91 44 38
EcoO109I rG'GnC_Cy
Cuts at: 0 168 285 354
Size: 168 117 69
Fragments arranged by size:
168 117 69
HaeII r_GCGC'y
Cuts at: 0 49 354
Size: 49 305
MspA1I CmG'CkG
Cuts at: 0 222 354
Size: 222 132
NaeI GCC'GGC
Cuts at: 0 92 279 354
Size: 92 187 75
Fragments arranged by size:
187 92 75
NgoMIV G'CCGG_C
Cuts at: 0 90 277 354
Size: 90 187 77
Fragments arranged by size:
187 90 77
PfoI T'CCnGG_A
Cuts at: 0 68 354
Size: 68 286
PpuMI rG'GwC_Cy
Cuts at: 0 285 354
Size: 285 69
PstI C_TGCA'G
Cuts at: 0 230 272 354
Size: 230 42 82
Fragments arranged by size:
230 82 42
SacI G_AGCT'C
Cuts at: 0 266 354
Size: 266 88
SfcI C'TryA_G
Cuts at: 0 226 268 354
Size: 226 42 86
Fragments arranged by size:
226 86 42
SmaI CCC'GGG
Cuts at: 0 173 354
Size: 173 181
StyI C'CwwG_G
Cuts at: 0 8 354
Size: 8 346
XmaI C'CCGG_G
Cuts at: 0 171 354
Size: 171 183
Enzymes that do cut:
AcuI AloI AlwNI AvaI BamHI BanII BbsI BbvCI
BglI BmrI BplI BpmI Bpu10I BsaAI BseRI BseYI
BsiHKAI Bsp1286I BsrBI BsrFI BstYI Bsu36I BtgI EcoICRI
Eco57MI EcoO109I HaeII MspA1I NaeI NgoMIV PfoI PpuMI
PstI SacI SfcI SmaI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BanI BbeI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI