Restriction map for
pF1KB0918
(Linear) (Six-base) MAPSORT of: KIBB0918.seq Check: 5219 from: 1 to: 993
KIBB0918.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 743 892 993
Size: 209 534 149 101
Fragments arranged by size:
534 209 149 101
AflII C'TTAA_G
Cuts at: 0 661 993
Size: 661 332
AflIII A'CryG_T
Cuts at: 0 130 993
Size: 130 863
AgeI A'CCGG_T
Cuts at: 0 91 993
Size: 91 902
ApaLI G'TGCA_C
Cuts at: 0 134 788 993
Size: 134 654 205
Fragments arranged by size:
654 205 134
ApoI r'AATT_y
Cuts at: 0 331 449 508 828 854 993
Size: 331 118 59 320 26 139
Fragments arranged by size:
331 320 139 118 59 26
AvaI C'yCGr_G
Cuts at: 0 25 993
Size: 25 968
BamHI G'GATC_C
Cuts at: 0 252 993
Size: 252 741
BanI G'GyrC_C
Cuts at: 0 40 993
Size: 40 953
BbeI G_GCGC'C
Cuts at: 0 44 993
Size: 44 949
BbsI GAAGACnn'nnnn_
Cuts at: 0 821 993
Size: 821 172
BfrBI ATG'CAT
Cuts at: 0 862 993
Size: 862 131
BglI GCCn_nnn'nGGC
Cuts at: 0 21 993
Size: 21 972
BglII A'GATC_T
Cuts at: 0 951 993
Size: 951 42
Bme1580I G_kGCm'C
Cuts at: 0 138 792 993
Size: 138 654 201
Fragments arranged by size:
654 201 138
BsaBI GATnn'nnATC
Cuts at: 0 251 993
Size: 251 742
BsaHI Gr'CG_yC
Cuts at: 0 10 41 993
Size: 10 31 952
Fragments arranged by size:
952 31 10
BsaWI w'CCGG_w
Cuts at: 0 91 993
Size: 91 902
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 341 371 993
Size: 341 30 622
Fragments arranged by size:
622 341 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 362 993
Size: 70 292 631
Fragments arranged by size:
631 292 70
BsiHKAI G_wGCw'C
Cuts at: 0 38 138 792 934 993
Size: 38 100 654 142 59
Fragments arranged by size:
654 142 100 59 38
Bsp1286I G_dGCh'C
Cuts at: 0 38 138 792 934 993
Size: 38 100 654 142 59
Fragments arranged by size:
654 142 100 59 38
BspHI T'CATG_A
Cuts at: 0 201 993
Size: 201 792
BsrFI r'CCGG_y
Cuts at: 0 91 993
Size: 91 902
BsrGI T'GTAC_A
Cuts at: 0 564 993
Size: 564 429
BstBI TT'CG_AA
Cuts at: 0 812 993
Size: 812 181
BstYI r'GATC_y
Cuts at: 0 252 951 993
Size: 252 699 42
Fragments arranged by size:
699 252 42
BtgI C'CryG_G
Cuts at: 0 356 993
Size: 356 637
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 477 993
Size: 477 516
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 856 891 993
Size: 856 35 102
Fragments arranged by size:
856 102 35
DraI TTT'AAA
Cuts at: 0 371 993
Size: 371 622
EarI CTCTTCn'nnn_
Cuts at: 0 611 887 993
Size: 611 276 106
Fragments arranged by size:
611 276 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 743 892 993
Size: 209 534 149 101
Fragments arranged by size:
534 209 149 101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 700 993
Size: 700 293
HaeII r_GCGC'y
Cuts at: 0 44 79 993
Size: 44 35 914
Fragments arranged by size:
914 44 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 480 512 943 975 993
Size: 271 32 177 32 431 32 18
Fragments arranged by size:
431 271 177 32 32 32 18
KasI G'GCGC_C
Cuts at: 0 40 993
Size: 40 953
MslI CAynn'nnrTG
Cuts at: 0 635 993
Size: 635 358
NarI GG'CG_CC
Cuts at: 0 41 993
Size: 41 952
NsiI A_TGCA'T
Cuts at: 0 864 993
Size: 864 129
NspI r_CATG'y
Cuts at: 0 134 478 993
Size: 134 344 515
Fragments arranged by size:
515 344 134
PciI A'CATG_T
Cuts at: 0 130 993
Size: 130 863
PsiI TTA'TAA
Cuts at: 0 455 993
Size: 455 538
SapI GCTCTTCn'nnn_
Cuts at: 0 611 993
Size: 611 382
SfcI C'TryA_G
Cuts at: 0 801 993
Size: 801 192
SfoI GGC'GCC
Cuts at: 0 42 993
Size: 42 951
SgrAI Cr'CCGG_yG
Cuts at: 0 91 993
Size: 91 902
SmlI C'TyrA_G
Cuts at: 0 661 993
Size: 661 332
SspI AAT'ATT
Cuts at: 0 710 993
Size: 710 283
TatI w'GTAC_w
Cuts at: 0 564 993
Size: 564 429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 341 373 993
Size: 341 32 620
Fragments arranged by size:
620 341 32
Enzymes that do cut:
AcuI AflII AflIII AgeI ApaLI ApoI AvaI BamHI
BanI BbeI BbsI BfrBI BglI BglII Bme1580I BsaBI
BsaHI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspHI BsrFI
BsrGI BstBI BstYI BtgI BtgZI CspCI DraI EarI
Eco57MI FalI HaeII Hin4I KasI MslI NarI NsiI
NspI PciI PsiI SapI SfcI SfoI SgrAI SmlI
SspI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AhdI AleI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BanII BbvCI BcgI BciVI BclI BlpI BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SmaI SnaBI SpeI SphI SrfI StuI StyI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI