Restriction map for
pF1KB0908
(Linear) (Six-base) MAPSORT of: KIBB0908.seq Check: 6497 from: 1 to: 933
KIBB0908.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 931 933
Size: 931 2
AccI GT'mk_AC
Cuts at: 0 438 662 933
Size: 438 224 271
Fragments arranged by size:
438 271 224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 933
Size: 124 809
AfeI AGC'GCT
Cuts at: 0 847 933
Size: 847 86
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 374 933
Size: 374 559
AlwNI CAG_nnn'CTG
Cuts at: 0 69 933
Size: 69 864
BamHI G'GATC_C
Cuts at: 0 282 933
Size: 282 651
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 75 109 933
Size: 75 34 824
Fragments arranged by size:
824 75 34
BclI T'GATC_A
Cuts at: 0 494 933
Size: 494 439
BglI GCCn_nnn'nGGC
Cuts at: 0 64 933
Size: 64 869
BlpI GC'TnA_GC
Cuts at: 0 449 933
Size: 449 484
BmgBI CAC'GTC
Cuts at: 0 9 933
Size: 9 924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 60 154 933
Size: 52 8 94 779
Fragments arranged by size:
779 94 52 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 293 933
Size: 293 640
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 933
Size: 310 623
BsmBI CGTCTCn'nnnn_
Cuts at: 0 121 933
Size: 121 812
BspHI T'CATG_A
Cuts at: 0 797 933
Size: 797 136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 931 933
Size: 931 2
BsrDI GCAATG_nn'
Cuts at: 0 569 933
Size: 569 364
BsrFI r'CCGG_y
Cuts at: 0 926 933
Size: 926 7
BstEII G'GTnAC_C
Cuts at: 0 573 933
Size: 573 360
BstYI r'GATC_y
Cuts at: 0 282 933
Size: 282 651
BtsI GCAGTG_nn'
Cuts at: 0 740 933
Size: 740 193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 444 479 933
Size: 444 35 454
Fragments arranged by size:
454 444 35
DraI TTT'AAA
Cuts at: 0 383 933
Size: 383 550
EarI CTCTTCn'nnn_
Cuts at: 0 260 933
Size: 260 673
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 933
Size: 19 914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 60 124 154 933
Size: 52 8 64 30 779
Fragments arranged by size:
779 64 52 30 8
EcoO109I rG'GnC_Cy
Cuts at: 0 195 834 933
Size: 195 639 99
Fragments arranged by size:
639 195 99
FspI TGC'GCA
Cuts at: 0 161 933
Size: 161 772
HaeII r_GCGC'y
Cuts at: 0 70 849 933
Size: 70 779 84
Fragments arranged by size:
779 84 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 676 708 933
Size: 676 32 225
Fragments arranged by size:
676 225 32
HincII GTy'rAC
Cuts at: 0 189 316 933
Size: 189 127 617
Fragments arranged by size:
617 189 127
HindIII A'AGCT_T
Cuts at: 0 679 933
Size: 679 254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 933
Size: 725 208
MslI CAynn'nnrTG
Cuts at: 0 263 333 933
Size: 263 70 600
Fragments arranged by size:
600 263 70
MspA1I CmG'CkG
Cuts at: 0 762 933
Size: 762 171
NaeI GCC'GGC
Cuts at: 0 928 933
Size: 928 5
NgoMIV G'CCGG_C
Cuts at: 0 926 933
Size: 926 7
PfoI T'CCnGG_A
Cuts at: 0 167 933
Size: 167 766
PpuMI rG'GwC_Cy
Cuts at: 0 195 933
Size: 195 738
PstI C_TGCA'G
Cuts at: 0 866 933
Size: 866 67
PvuII CAG'CTG
Cuts at: 0 762 933
Size: 762 171
SexAI A'CCwGG_T
Cuts at: 0 802 933
Size: 802 131
SfcI C'TryA_G
Cuts at: 0 309 862 933
Size: 309 553 71
Fragments arranged by size:
553 309 71
StuI AGG'CCT
Cuts at: 0 37 151 933
Size: 37 114 782
Fragments arranged by size:
782 114 37
StyI C'CwwG_G
Cuts at: 0 578 933
Size: 578 355
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 174 933
Size: 174 759
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AlwNI BamHI BcgI
BclI BglI BlpI BmgBI BpmI BseRI BsgI BsmBI
BspHI BspMI BsrDI BsrFI BstEII BstYI BtsI CspCI
DraI EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I
HincII HindIII MmeI MslI MspA1I NaeI NgoMIV PfoI
PpuMI PstI PvuII SexAI SfcI StuI StyI TaqII
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BbsI BbvCI BciVI
BfrBI BglII Bme1580I BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI HpaI KasI KpnI MfeI
MluI MscI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI