(Linear) (Six-base) MAPSORT of: KIBB0905.seq  Check: 3464  from: 1  to: 279



KIBB0905.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AvrII C'CTAG_G

Cuts at:      0      51     279 
   Size:         51     228
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     186     279 
   Size:        186      93
 
BglII A'GATC_T

Cuts at:      0       5     279 
   Size:          5     274
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     227     279 
   Size:        227      52
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     154     279 
   Size:        154     125
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25      63     279 
   Size:         25      38     216

  Fragments arranged by size: 

                 216      38      25
 
BsiHKAI G_wGCw'C

Cuts at:      0     178     279 
   Size:        178     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     178     279 
   Size:        178     101
 
BstYI r'GATC_y

Cuts at:      0       5     128     279 
   Size:          5     123     151

  Fragments arranged by size: 

                 151     123       5
 
BtgI C'CryG_G

Cuts at:      0      11     279 
   Size:         11     268
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      30     279 
   Size:         30     249
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     244     279 
   Size:        244      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0      82     279 
   Size:         82     197
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     120     152     279 
   Size:        120      32     127

  Fragments arranged by size: 

                 127     120      32
 
MspA1I CmG'CkG

Cuts at:      0     141     279 
   Size:        141     138
 
PfoI T'CCnGG_A

Cuts at:      0     248     279 
   Size:        248      31
 
PpuMI rG'GwC_Cy

Cuts at:      0      82     279 
   Size:         82     197
 
StyI C'CwwG_G

Cuts at:      0      51     279 
   Size:         51     228
 

 Enzymes that do cut:

    AvrII     BbsI    BglII     BmrI     BsaI    BseRI  BsiHKAI Bsp1286I
    BstYI     BtgI    BtgZI     DrdI EcoO109I    Hin4I   MspA1I     PfoI
    PpuMI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI