(Linear) (Six-base) MAPSORT of: KIBB0487.seq  Check: 7726  from: 1  to: 1587



KIBB0487.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:      0     473    1587 
   Size:        473    1114
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247    1587 
   Size:        247    1340
 
AfeI AGC'GCT

Cuts at:      0     462    1587 
   Size:        462    1125
 
AleI CACnn'nnGTG

Cuts at:      0     778    1265    1587 
   Size:        778     487     322

  Fragments arranged by size: 

                 778     487     322
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     778    1587 
   Size:        778     809
 
AlwNI CAG_nnn'CTG

Cuts at:      0     268    1587 
   Size:        268    1319
 
ApoI r'AATT_y

Cuts at:      0      91     145     353     918    1587 
   Size:         91      54     208     565     669

  Fragments arranged by size: 

                 669     565     208      91      54
 
AseI AT'TA_AT

Cuts at:      0     376    1587 
   Size:        376    1211
 
BanII G_rGCy'C

Cuts at:      0     984    1587 
   Size:        984     603
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1202    1282    1587 
   Size:       1202      80     305

  Fragments arranged by size: 

                1202     305      80
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     147     328     993    1587 
   Size:        147     181     665     594

  Fragments arranged by size: 

                 665     594     181     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86    1587 
   Size:         86    1501
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1021    1587 
   Size:       1021     566
 
BsaAI yAC'GTr

Cuts at:      0     397    1587 
   Size:        397    1190
 
BsaBI GATnn'nnATC

Cuts at:      0     787    1587 
   Size:        787     800
 
BseYI C'CCAG_C

Cuts at:      0     806    1587 
   Size:        806     781
 
BsiHKAI G_wGCw'C

Cuts at:      0     888    1587 
   Size:        888     699
 
BsmI GAATG_Cn'

Cuts at:      0     175    1085    1587 
   Size:        175     910     502

  Fragments arranged by size: 

                 910     502     175
 
Bsp1286I G_dGCh'C

Cuts at:      0     888     984    1587 
   Size:        888      96     603

  Fragments arranged by size: 

                 888     603      96
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     895    1587 
   Size:        895     692
 
BsrDI GCAATG_nn'

Cuts at:      0     842    1151    1587 
   Size:        842     309     436

  Fragments arranged by size: 

                 842     436     309
 
BssSI C'ACGA_G

Cuts at:      0    1036    1587 
   Size:       1036     551
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     558    1587 
   Size:        558    1029
 
DraI TTT'AAA

Cuts at:      0     917    1587 
   Size:        917     670
 
EaeI y'GGCC_r

Cuts at:      0     839     923    1587 
   Size:        839      84     664

  Fragments arranged by size: 

                 839     664      84
 
EarI CTCTTCn'nnn_

Cuts at:      0    1403    1587 
   Size:       1403     184
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     247    1587 
   Size:         86     161    1340

  Fragments arranged by size: 

                1340     161      86
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     578    1587 
   Size:        578    1009
 
EcoRI G'AATT_C

Cuts at:      0     353    1587 
   Size:        353    1234
 
EcoRV GAT'ATC

Cuts at:      0     719    1587 
   Size:        719     868
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1516    1587 
   Size:       1516      71
 
HaeII r_GCGC'y

Cuts at:      0     464    1587 
   Size:        464    1123
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1269    1301    1587 
   Size:       1269      32     286

  Fragments arranged by size: 

                1269     286      32
 
HindIII A'AGCT_T

Cuts at:      0     661    1587 
   Size:        661     926
 
MfeI C'AATT_G

Cuts at:      0    1532    1587 
   Size:       1532      55
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     861    1333    1587 
   Size:        861     472     254

  Fragments arranged by size: 

                 861     472     254
 
MscI TGG'CCA

Cuts at:      0     841     925    1587 
   Size:        841      84     662

  Fragments arranged by size: 

                 841     662      84
 
MslI CAynn'nnrTG

Cuts at:      0     778    1265    1587 
   Size:        778     487     322

  Fragments arranged by size: 

                 778     487     322
 
MspA1I CmG'CkG

Cuts at:      0     213     258     709    1587 
   Size:        213      45     451     878

  Fragments arranged by size: 

                 878     451     213      45
 
NspI r_CATG'y

Cuts at:      0     934    1587 
   Size:        934     653
 
PasI CC'CwG_GG

Cuts at:      0     382    1587 
   Size:        382    1205
 
PmlI CAC'GTG

Cuts at:      0     397    1587 
   Size:        397    1190
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     637     669    1587 
   Size:        637      32     918

  Fragments arranged by size: 

                 918     637      32
 
PstI C_TGCA'G

Cuts at:      0     714    1587 
   Size:        714     873
 
PvuII CAG'CTG

Cuts at:      0     213     709    1587 
   Size:        213     496     878

  Fragments arranged by size: 

                 878     496     213
 
SfcI C'TryA_G

Cuts at:      0     710    1587 
   Size:        710     877
 
SphI G_CATG'C

Cuts at:      0     934    1587 
   Size:        934     653
 
SspI AAT'ATT

Cuts at:      0     759    1587 
   Size:        759     828
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     790     864    1587 
   Size:        790      74     723

  Fragments arranged by size: 

                 790     723      74
 
XbaI T'CTAG_A

Cuts at:      0     991    1587 
   Size:        991     596
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1098    1587 
   Size:       1098     489
 
XmnI GAAnn'nnTTC

Cuts at:      0     357    1071    1587 
   Size:        357     714     516

  Fragments arranged by size: 

                 714     516     357
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AleI     AlfI    AlwNI     ApoI     AseI
    BanII     BbsI    BciVI     BpmI     BsaI    BsaAI    BsaBI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BssSI    BtgZI     DraI
     EaeI     EarI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI    HaeII
    Hin4I  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NspI
     PasI     PmlI     PpiI     PstI    PvuII     SfcI     SphI     SspI
    TaqII     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XhoI     XmaI     ZraI