Restriction map for
pF1KB0476
(Linear) (Six-base) MAPSORT of: KIBB0476.seq Check: 8484 from: 1 to: 3057
KIBB0476.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 0 2676 3057
Size: 2676 381
Acc65I G'GTAC_C
Cuts at: 0 5 3057
Size: 5 3052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 627 848 949 2093 2484 2987 3057
Size: 194 433 221 101 1144 391 503 70
Fragments arranged by size:
1144 503 433 391 221 194 101 70
AflII C'TTAA_G
Cuts at: 0 315 1048 2134 3057
Size: 315 733 1086 923
Fragments arranged by size:
1086 923 733 315
AflIII A'CryG_T
Cuts at: 0 1155 1178 3057
Size: 1155 23 1879
Fragments arranged by size:
1879 1155 23
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 860 892 3057
Size: 860 32 2165
Fragments arranged by size:
2165 860 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2157 3057
Size: 2157 900
ApaLI G'TGCA_C
Cuts at: 0 274 3057
Size: 274 2783
ApoI r'AATT_y
Cuts at: 0 649 727 859 1372 1561 1585 1599 1729
Size: 649 78 132 513 189 24 14 130
Cuts at: 1729 2812 2934 2989 3057
Size: 1083 122 55 68
Fragments arranged by size:
1083 649 513 189 132 130 122 78
68 55 24 14
AseI AT'TA_AT
Cuts at: 0 1124 3057
Size: 1124 1933
AvaI C'yCGr_G
Cuts at: 0 1100 3057
Size: 1100 1957
BamHI G'GATC_C
Cuts at: 0 294 3057
Size: 294 2763
BanI G'GyrC_C
Cuts at: 0 5 64 478 3057
Size: 5 59 414 2579
Fragments arranged by size:
2579 414 59 5
BanII G_rGCy'C
Cuts at: 0 1101 1796 2429 3057
Size: 1101 695 633 628
Fragments arranged by size:
1101 695 633 628
BbeI G_GCGC'C
Cuts at: 0 68 3057
Size: 68 2989
BbsI GAAGACnn'nnnn_
Cuts at: 0 182 2243 2490 3057
Size: 182 2061 247 567
Fragments arranged by size:
2061 567 247 182
BbvCI CC'TCA_GC
Cuts at: 0 2150 3057
Size: 2150 907
BciVI GTATCCnnnnn_n'
Cuts at: 0 1650 2871 3057
Size: 1650 1221 186
Fragments arranged by size:
1650 1221 186
BfrBI ATG'CAT
Cuts at: 0 1822 3057
Size: 1822 1235
Bme1580I G_kGCm'C
Cuts at: 0 278 483 514 3057
Size: 278 205 31 2543
Fragments arranged by size:
2543 278 205 31
BmgBI CAC'GTC
Cuts at: 0 2512 3057
Size: 2512 545
BmrI ACTGGGnnnn_n'
Cuts at: 0 1488 2730 3057
Size: 1488 1242 327
Fragments arranged by size:
1488 1242 327
BmtI G_CTAG'C
Cuts at: 0 16 1840 3057
Size: 16 1824 1217
Fragments arranged by size:
1824 1217 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 697 2443 3057
Size: 615 82 1746 614
Fragments arranged by size:
1746 615 614 82
Bpu10I CC'TnA_GC
Cuts at: 0 2150 3057
Size: 2150 907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1202 1810 1866 2556 3057
Size: 1202 608 56 690 501
Fragments arranged by size:
1202 690 608 501 56
BsaAI yAC'GTr
Cuts at: 0 1204 3057
Size: 1204 1853
BsaHI Gr'CG_yC
Cuts at: 0 65 3057
Size: 65 2992
BsaWI w'CCGG_w
Cuts at: 0 81 1515 3057
Size: 81 1434 1542
Fragments arranged by size:
1542 1434 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 153 183 2138 2168 3057
Size: 76 30 47 30 1955 30 889
Fragments arranged by size:
1955 889 76 47 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1786 3057
Size: 1786 1271
BseYI C'CCAG_C
Cuts at: 0 40 1061 2738 3057
Size: 40 1021 1677 319
Fragments arranged by size:
1677 1021 319 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 1226 2152 3057
Size: 10 1216 926 905
Fragments arranged by size:
1216 926 905 10
BsiHKAI G_wGCw'C
Cuts at: 0 278 1349 1796 2429 3057
Size: 278 1071 447 633 628
Fragments arranged by size:
1071 633 628 447 278
BsmI GAATG_Cn'
Cuts at: 0 555 1257 3057
Size: 555 702 1800
Fragments arranged by size:
1800 702 555
Bsp1286I G_dGCh'C
Cuts at: 0 278 483 514 1101 1349 1796 2429 3057
Size: 278 205 31 587 248 447 633 628
Fragments arranged by size:
633 628 587 447 278 248 205 31
BspEI T'CCGG_A
Cuts at: 0 81 3057
Size: 81 2976
BspHI T'CATG_A
Cuts at: 0 462 3057
Size: 462 2595
BsrBI CCG'CTC
Cuts at: 0 53 3057
Size: 53 3004
BsrDI GCAATG_nn'
Cuts at: 0 850 3057
Size: 850 2207
BsrFI r'CCGG_y
Cuts at: 0 8 3057
Size: 8 3049
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2839 3057
Size: 2839 218
BstXI CCAn_nnnn'nTGG
Cuts at: 0 759 2886 3057
Size: 759 2127 171
Fragments arranged by size:
2127 759 171
BstYI r'GATC_y
Cuts at: 0 294 3057
Size: 294 2763
BtgI C'CryG_G
Cuts at: 0 245 1288 2187 2606 3057
Size: 245 1043 899 419 451
Fragments arranged by size:
1043 899 451 419 245
BtsI GCAGTG_nn'
Cuts at: 0 34 3057
Size: 34 3023
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2346 2381 3057
Size: 2346 35 676
Fragments arranged by size:
2346 676 35
DraI TTT'AAA
Cuts at: 0 1272 1566 2783 3057
Size: 1272 294 1217 274
Fragments arranged by size:
1272 1217 294 274
EaeI y'GGCC_r
Cuts at: 0 206 3057
Size: 206 2851
EarI CTCTTCn'nnn_
Cuts at: 0 1334 1472 2434 2630 3057
Size: 1334 138 962 196 427
Fragments arranged by size:
1334 962 427 196 138
EcoICRI GAG'CTC
Cuts at: 0 1794 2427 3057
Size: 1794 633 630
Fragments arranged by size:
1794 633 630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 615 627 697 848 949 2093 2443
Size: 194 421 12 70 151 101 1144 350
Cuts at: 2443 2484 2987 3057
Size: 41 503 70
Fragments arranged by size:
1144 503 421 350 194 151 101 70
70 41 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1339 2318 3057
Size: 1339 979 739
Fragments arranged by size:
1339 979 739
EcoO109I rG'GnC_Cy
Cuts at: 0 1024 1213 3057
Size: 1024 189 1844
Fragments arranged by size:
1844 1024 189
EcoRI G'AATT_C
Cuts at: 0 2989 3057
Size: 2989 68
EcoRV GAT'ATC
Cuts at: 0 2805 3057
Size: 2805 252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 244 907 3057
Size: 244 663 2150
Fragments arranged by size:
2150 663 244
FspI TGC'GCA
Cuts at: 0 2470 3057
Size: 2470 587
HaeII r_GCGC'y
Cuts at: 0 68 90 3057
Size: 68 22 2967
Fragments arranged by size:
2967 68 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 1839 1871 2531 2563 2717 2749
Size: 241 32 1566 32 660 32 154 32
Cuts at: 2749 3057
Size: 308
Fragments arranged by size:
1566 660 308 241 154 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2677 3057
Size: 2677 380
HindIII A'AGCT_T
Cuts at: 0 2591 3057
Size: 2591 466
KasI G'GCGC_C
Cuts at: 0 64 3057
Size: 64 2993
KpnI G_GTAC'C
Cuts at: 0 9 3057
Size: 9 3048
MfeI C'AATT_G
Cuts at: 0 607 3057
Size: 607 2450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 776 2886 3057
Size: 272 504 2110 171
Fragments arranged by size:
2110 504 272 171
MscI TGG'CCA
Cuts at: 0 208 3057
Size: 208 2849
MslI CAynn'nnrTG
Cuts at: 0 326 1126 2039 3057
Size: 326 800 913 1018
Fragments arranged by size:
1018 913 800 326
MspA1I CmG'CkG
Cuts at: 0 1354 2286 2504 3057
Size: 1354 932 218 553
Fragments arranged by size:
1354 932 553 218
NarI GG'CG_CC
Cuts at: 0 65 3057
Size: 65 2992
NcoI C'CATG_G
Cuts at: 0 2187 2606 3057
Size: 2187 419 451
Fragments arranged by size:
2187 451 419
NdeI CA'TA_TG
Cuts at: 0 335 1824 3057
Size: 335 1489 1233
Fragments arranged by size:
1489 1233 335
NheI G'CTAG_C
Cuts at: 0 12 1836 3057
Size: 12 1824 1221
Fragments arranged by size:
1824 1221 12
NsiI A_TGCA'T
Cuts at: 0 1824 3057
Size: 1824 1233
NspI r_CATG'y
Cuts at: 0 403 1159 1182 2044 2257 2397 3057
Size: 403 756 23 862 213 140 660
Fragments arranged by size:
862 756 660 403 213 140 23
PciI A'CATG_T
Cuts at: 0 1155 1178 3057
Size: 1155 23 1879
Fragments arranged by size:
1879 1155 23
PfoI T'CCnGG_A
Cuts at: 0 1026 3057
Size: 1026 2031
PpuMI rG'GwC_Cy
Cuts at: 0 1024 1213 3057
Size: 1024 189 1844
Fragments arranged by size:
1844 1024 189
PvuII CAG'CTG
Cuts at: 0 2286 3057
Size: 2286 771
SacI G_AGCT'C
Cuts at: 0 1796 2429 3057
Size: 1796 633 628
Fragments arranged by size:
1796 633 628
SalI G'TCGA_C
Cuts at: 0 2675 3057
Size: 2675 382
SapI GCTCTTCn'nnn_
Cuts at: 0 2434 3057
Size: 2434 623
ScaI AGT'ACT
Cuts at: 0 224 3057
Size: 224 2833
SfcI C'TryA_G
Cuts at: 0 620 3057
Size: 620 2437
SfoI GGC'GCC
Cuts at: 0 66 3057
Size: 66 2991
SmlI C'TyrA_G
Cuts at: 0 315 1048 1217 1789 1845 2134 2535 3057
Size: 315 733 169 572 56 289 401 522
Fragments arranged by size:
733 572 522 401 315 289 169 56
SpeI A'CTAG_T
Cuts at: 0 424 3057
Size: 424 2633
SphI G_CATG'C
Cuts at: 0 403 2044 3057
Size: 403 1641 1013
Fragments arranged by size:
1641 1013 403
SspI AAT'ATT
Cuts at: 0 306 1319 1360 3057
Size: 306 1013 41 1697
Fragments arranged by size:
1697 1013 306 41
StuI AGG'CCT
Cuts at: 0 2140 2641 3057
Size: 2140 501 416
Fragments arranged by size:
2140 501 416
StyI C'CwwG_G
Cuts at: 0 2187 2474 2606 2795 2845 3057
Size: 2187 287 132 189 50 212
Fragments arranged by size:
2187 287 212 189 132 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1153 3001 3057
Size: 1153 1848 56
Fragments arranged by size:
1848 1153 56
TatI w'GTAC_w
Cuts at: 0 222 1496 2048 3057
Size: 222 1274 552 1009
Fragments arranged by size:
1274 1009 552 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 76 108 3057
Size: 76 32 2949
Fragments arranged by size:
2949 76 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2481 3057
Size: 2481 576
XmnI GAAnn'nnTTC
Cuts at: 0 1589 2180 2972 3057
Size: 1589 591 792 85
Fragments arranged by size:
1589 792 591 85
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AloI AlwNI ApaLI
ApoI AseI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BfrBI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BsrDI
BsrFI BstAPI BstXI BstYI BtgI BtsI CspCI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NsiI NspI PciI PfoI PpuMI PvuII SacI
SalI SapI ScaI SfcI SfoI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AleI AlfI
ApaI AscI AsiSI AvrII BaeI BcgI BclI BglI
BglII BlpI BplI BsaI BsaBI BsiEI BsiWI BsmBI
BspMI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraIII DrdI EagI EciI FseI FspAI
HpaI MluI NaeI NgoMIV NotI NruI PacI PasI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SanDI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SrfI SwaI Tth111I
XbaI XhoI XmaI ZraI