Restriction map for
pF1KB0465
(Linear) (Six-base) MAPSORT of: KIBB0465.seq Check: 7232 from: 1 to: 1434
KIBB0465.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AatII G_ACGT'C
Cuts at: 0 194 1434
Size: 194 1240
AccI GT'mk_AC
Cuts at: 0 920 1434
Size: 920 514
Acc65I G'GTAC_C
Cuts at: 0 254 1434
Size: 254 1180
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1434
Size: 436 998
AflIII A'CryG_T
Cuts at: 0 407 1434
Size: 407 1027
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 958 1434
Size: 958 476
AlwNI CAG_nnn'CTG
Cuts at: 0 24 237 344 1263 1434
Size: 24 213 107 919 171
Fragments arranged by size:
919 213 171 107 24
ApaLI G'TGCA_C
Cuts at: 0 988 1434
Size: 988 446
AvaI C'yCGr_G
Cuts at: 0 1191 1223 1316 1434
Size: 1191 32 93 118
Fragments arranged by size:
1191 118 93 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 164 197 1434
Size: 164 33 1237
Fragments arranged by size:
1237 164 33
BanI G'GyrC_C
Cuts at: 0 254 1434
Size: 254 1180
BanII G_rGCy'C
Cuts at: 0 1192 1339 1434
Size: 1192 147 95
Fragments arranged by size:
1192 147 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 597 849 1434
Size: 597 252 585
Fragments arranged by size:
597 585 252
BbvCI CC'TCA_GC
Cuts at: 0 103 1434
Size: 103 1331
BciVI GTATCCnnnnn_n'
Cuts at: 0 192 1434
Size: 192 1242
BclI T'GATC_A
Cuts at: 0 863 1434
Size: 863 571
BglI GCCn_nnn'nGGC
Cuts at: 0 1023 1237 1434
Size: 1023 214 197
Fragments arranged by size:
1023 214 197
BlpI GC'TnA_GC
Cuts at: 0 1134 1434
Size: 1134 300
Bme1580I G_kGCm'C
Cuts at: 0 992 1434
Size: 992 442
BmtI G_CTAG'C
Cuts at: 0 1378 1434
Size: 1378 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 837 1284 1434
Size: 837 447 150
Fragments arranged by size:
837 447 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 268 1063 1434
Size: 183 85 795 371
Fragments arranged by size:
795 371 183 85
Bpu10I CC'TnA_GC
Cuts at: 0 103 1434
Size: 103 1331
BsaI GGTCTCn'nnnn_
Cuts at: 0 825 958 1434
Size: 825 133 476
Fragments arranged by size:
825 476 133
BsaAI yAC'GTr
Cuts at: 0 408 1434
Size: 408 1026
BsaHI Gr'CG_yC
Cuts at: 0 191 1434
Size: 191 1243
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 87 829 896 1434
Size: 28 59 742 67 538
Fragments arranged by size:
742 538 67 59 28
BseYI C'CCAG_C
Cuts at: 0 633 754 1098 1434
Size: 633 121 344 336
Fragments arranged by size:
633 344 336 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1434
Size: 520 914
BsiHKAI G_wGCw'C
Cuts at: 0 992 1434
Size: 992 442
BsiWI C'GTAC_G
Cuts at: 0 404 1434
Size: 404 1030
Bsp1286I G_dGCh'C
Cuts at: 0 992 1192 1339 1434
Size: 992 200 147 95
Fragments arranged by size:
992 200 147 95
BspHI T'CATG_A
Cuts at: 0 608 1434
Size: 608 826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 803 1010 1379 1434
Size: 803 207 369 55
Fragments arranged by size:
803 369 207 55
BsrBI CCG'CTC
Cuts at: 0 97 1434
Size: 97 1337
BsrFI r'CCGG_y
Cuts at: 0 257 716 885 1434
Size: 257 459 169 549
Fragments arranged by size:
549 459 257 169
BstAPI GCAn_nnn'nTGC
Cuts at: 0 24 1002 1014 1434
Size: 24 978 12 420
Fragments arranged by size:
978 420 24 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 1434
Size: 51 1383
BstYI r'GATC_y
Cuts at: 0 1301 1434
Size: 1301 133
Bsu36I CC'TnA_GG
Cuts at: 0 296 1069 1434
Size: 296 773 365
Fragments arranged by size:
773 365 296
BtgI C'CryG_G
Cuts at: 0 332 1139 1434
Size: 332 807 295
Fragments arranged by size:
807 332 295
BtsI GCAGTG_nn'
Cuts at: 0 890 1149 1434
Size: 890 259 285
Fragments arranged by size:
890 285 259
DrdI GACnn_nn'nnGTC
Cuts at: 0 196 601 1434
Size: 196 405 833
Fragments arranged by size:
833 405 196
EaeI y'GGCC_r
Cuts at: 0 887 959 1434
Size: 887 72 475
Fragments arranged by size:
887 475 72
EarI CTCTTCn'nnn_
Cuts at: 0 733 1180 1434
Size: 733 447 254
Fragments arranged by size:
733 447 254
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 703 1434
Size: 703 731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 268 436 1063 1434
Size: 183 85 168 627 371
Fragments arranged by size:
627 371 183 168 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 1434
Size: 55 1379
EcoO109I rG'GnC_Cy
Cuts at: 0 348 1094 1168 1434
Size: 348 746 74 266
Fragments arranged by size:
746 348 266 74
HaeII r_GCGC'y
Cuts at: 0 25 573 1434
Size: 25 548 861
Fragments arranged by size:
861 548 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 1434
Size: 1117 32 285
Fragments arranged by size:
1117 285 32
HincII GTy'rAC
Cuts at: 0 1082 1434
Size: 1082 352
KpnI G_GTAC'C
Cuts at: 0 258 1434
Size: 258 1176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 617 1434
Size: 92 525 817
Fragments arranged by size:
817 525 92
MslI CAynn'nnrTG
Cuts at: 0 772 1434
Size: 772 662
NaeI GCC'GGC
Cuts at: 0 718 887 1434
Size: 718 169 547
Fragments arranged by size:
718 547 169
NcoI C'CATG_G
Cuts at: 0 1139 1434
Size: 1139 295
NgoMIV G'CCGG_C
Cuts at: 0 716 885 1434
Size: 716 169 549
Fragments arranged by size:
716 549 169
NheI G'CTAG_C
Cuts at: 0 1374 1434
Size: 1374 60
PfoI T'CCnGG_A
Cuts at: 0 350 545 1283 1434
Size: 350 195 738 151
Fragments arranged by size:
738 350 195 151
PpuMI rG'GwC_Cy
Cuts at: 0 348 1168 1434
Size: 348 820 266
Fragments arranged by size:
820 348 266
PstI C_TGCA'G
Cuts at: 0 1007 1156 1259 1434
Size: 1007 149 103 175
Fragments arranged by size:
1007 175 149 103
SanDI GG'GwC_CC
Cuts at: 0 348 1434
Size: 348 1086
SapI GCTCTTCn'nnn_
Cuts at: 0 1180 1434
Size: 1180 254
ScaI AGT'ACT
Cuts at: 0 265 1434
Size: 265 1169
SexAI A'CCwGG_T
Cuts at: 0 1034 1434
Size: 1034 400
SfcI C'TryA_G
Cuts at: 0 132 468 1003 1152 1255 1389 1434
Size: 132 336 535 149 103 134 45
Fragments arranged by size:
535 336 149 134 132 103 45
SgrAI Cr'CCGG_yG
Cuts at: 0 716 1434
Size: 716 718
StuI AGG'CCT
Cuts at: 0 1231 1434
Size: 1231 203
StyI C'CwwG_G
Cuts at: 0 1139 1434
Size: 1139 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 843 1434
Size: 843 591
TatI w'GTAC_w
Cuts at: 0 263 1434
Size: 263 1171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 213 1434
Size: 213 1221
ZraI GAC'GTC
Cuts at: 0 192 1434
Size: 192 1242
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AlfI AlwNI ApaLI
AvaI BaeI BanI BanII BbsI BbvCI BciVI BclI
BglI BlpI Bme1580I BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsiWI Bsp1286I
BspHI BspMI BsrBI BsrFI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KpnI MmeI MslI NaeI
NcoI NgoMIV NheI PfoI PpuMI PstI SanDI SapI
ScaI SexAI SfcI SgrAI StuI StyI TaqII TatI
XcmI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AleI AloI
ApaI ApoI AscI AseI AsiSI AvrII BamHI BbeI
BcgI BfrBI BglII BmgBI BmrI BpuEI BsaBI BsaWI
BsaXI BsiEI BsmI BsmBI BspEI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI MfeI MluI MscI MspA1I
NarI NdeI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SbfI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI