Restriction map for
pF1KB0462
(Linear) (Six-base) MAPSORT of: KIBB0462.seq Check: 2134 from: 1 to: 2928
KIBB0462.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1679 1788 2454 2928
Size: 1679 109 666 474
Fragments arranged by size:
1679 666 474 109
AatII G_ACGT'C
Cuts at: 0 29 2928
Size: 29 2899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 1547 1937 2119 2662 2928
Size: 1342 205 390 182 543 266
Fragments arranged by size:
1342 543 390 266 205 182
AfeI AGC'GCT
Cuts at: 0 19 131 2928
Size: 19 112 2797
Fragments arranged by size:
2797 112 19
AflIII A'CryG_T
Cuts at: 0 2609 2928
Size: 2609 319
AgeI A'CCGG_T
Cuts at: 0 982 2928
Size: 982 1946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 939 2718 2928
Size: 939 1779 210
Fragments arranged by size:
1779 939 210
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 739 771 2928
Size: 739 32 2157
Fragments arranged by size:
2157 739 32
AlwNI CAG_nnn'CTG
Cuts at: 0 260 600 825 1167 2337 2928
Size: 260 340 225 342 1170 591
Fragments arranged by size:
1170 591 342 340 260 225
ApaI G_GGCC'C
Cuts at: 0 368 2928
Size: 368 2560
ApaLI G'TGCA_C
Cuts at: 0 169 2928
Size: 169 2759
ApoI r'AATT_y
Cuts at: 0 1385 2775 2928
Size: 1385 1390 153
Fragments arranged by size:
1390 1385 153
AvaI C'yCGr_G
Cuts at: 0 1741 2014 2437 2648 2910 2928
Size: 1741 273 423 211 262 18
Fragments arranged by size:
1741 423 273 262 211 18
BamHI G'GATC_C
Cuts at: 0 378 1328 2928
Size: 378 950 1600
Fragments arranged by size:
1600 950 378
BanI G'GyrC_C
Cuts at: 0 543 810 985 1667 1860 2718 2928
Size: 543 267 175 682 193 858 210
Fragments arranged by size:
858 682 543 267 210 193 175
BanII G_rGCy'C
Cuts at: 0 368 905 974 1853 2045 2928
Size: 368 537 69 879 192 883
Fragments arranged by size:
883 879 537 368 192 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 1535 2792 2928
Size: 1535 1257 136
Fragments arranged by size:
1535 1257 136
BbvCI CC'TCA_GC
Cuts at: 0 1567 2928
Size: 1567 1361
BciVI GTATCCnnnnn_n'
Cuts at: 0 1360 2928
Size: 1360 1568
BclI T'GATC_A
Cuts at: 0 2273 2928
Size: 2273 655
BglI GCCn_nnn'nGGC
Cuts at: 0 154 655 794 1026 1035 2928
Size: 154 501 139 232 9 1893
Fragments arranged by size:
1893 501 232 154 139 9
BglII A'GATC_T
Cuts at: 0 2849 2928
Size: 2849 79
BlpI GC'TnA_GC
Cuts at: 0 126 275 2928
Size: 126 149 2653
Fragments arranged by size:
2653 149 126
Bme1580I G_kGCm'C
Cuts at: 0 173 199 368 990 1223 1670 2928
Size: 173 26 169 622 233 447 1258
Fragments arranged by size:
1258 622 447 233 173 169 26
BmgBI CAC'GTC
Cuts at: 0 1776 2928
Size: 1776 1152
BmrI ACTGGGnnnn_n'
Cuts at: 0 1034 2928
Size: 1034 1894
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1470 2928
Size: 1470 1458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1354 1447 1996 2359 2650 2928
Size: 1354 93 549 363 291 278
Fragments arranged by size:
1354 549 363 291 278 93
Bpu10I CC'TnA_GC
Cuts at: 0 568 1567 2928
Size: 568 999 1361
Fragments arranged by size:
1361 999 568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 727 889 2928
Size: 679 48 162 2039
Fragments arranged by size:
2039 679 162 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 1470 2769 2928
Size: 1470 1299 159
Fragments arranged by size:
1470 1299 159
BsaAI yAC'GTr
Cuts at: 0 2612 2928
Size: 2612 316
BsaBI GATnn'nnATC
Cuts at: 0 2149 2928
Size: 2149 779
BsaHI Gr'CG_yC
Cuts at: 0 26 1112 2426 2558 2928
Size: 26 1086 1314 132 370
Fragments arranged by size:
1314 1086 370 132 26
BsaWI w'CCGG_w
Cuts at: 0 381 982 1226 1964 2597 2928
Size: 381 601 244 738 633 331
Fragments arranged by size:
738 633 601 381 331 244
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 739 769 1882 1912 2251 2281
Size: 226 30 483 30 1113 30 339 30
Cuts at: 2281 2928
Size: 647
Fragments arranged by size:
1113 647 483 339 226 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 834 837 1351 1759 1906 2775 2784
Size: 508 326 3 514 408 147 869 9
Cuts at: 2784 2928
Size: 144
Fragments arranged by size:
869 514 508 408 326 147 144 9
3
BseYI C'CCAG_C
Cuts at: 0 9 296 937 1492 1557 2928
Size: 9 287 641 555 65 1371
Fragments arranged by size:
1371 641 555 287 65 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 613 1180 1284 2923 2928
Size: 427 186 567 104 1639 5
Fragments arranged by size:
1639 567 427 186 104 5
BsiEI CG_ry'CG
Cuts at: 0 54 2518 2928
Size: 54 2464 410
Fragments arranged by size:
2464 410 54
BsiHKAI G_wGCw'C
Cuts at: 0 127 173 329 348 395 905 2928
Size: 127 46 156 19 47 510 2023
Fragments arranged by size:
2023 510 156 127 47 46 19
BsiWI C'GTAC_G
Cuts at: 0 2612 2928
Size: 2612 316
BsmI GAATG_Cn'
Cuts at: 0 228 1070 1503 2738 2928
Size: 228 842 433 1235 190
Fragments arranged by size:
1235 842 433 228 190
BsmBI CGTCTCn'nnnn_
Cuts at: 0 312 2787 2928
Size: 312 2475 141
Fragments arranged by size:
2475 312 141
Bsp1286I G_dGCh'C
Cuts at: 0 127 173 199 329 348 368 395 905
Size: 127 46 26 130 19 20 27 510
Cuts at: 905 974 990 1223 1670 1853 2045 2928
Size: 69 16 233 447 183 192 883
Fragments arranged by size:
883 510 447 233 192 183 130 127
69 46 27 26 20 19 16
BspEI T'CCGG_A
Cuts at: 0 381 1964 2597 2928
Size: 381 1583 633 331
Fragments arranged by size:
1583 633 381 331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 833 1679 1788 2163 2454 2928
Size: 833 846 109 375 291 474
Fragments arranged by size:
846 833 474 375 291 109
BsrDI GCAATG_nn'
Cuts at: 0 2712 2869 2928
Size: 2712 157 59
Fragments arranged by size:
2712 157 59
BsrFI r'CCGG_y
Cuts at: 0 982 2513 2517 2624 2890 2928
Size: 982 1531 4 107 266 38
Fragments arranged by size:
1531 982 266 107 38 4
BsrGI T'GTAC_A
Cuts at: 0 866 2873 2928
Size: 866 2007 55
Fragments arranged by size:
2007 866 55
BssSI C'ACGA_G
Cuts at: 0 100 2857 2928
Size: 100 2757 71
Fragments arranged by size:
2757 100 71
BstAPI GCAn_nnn'nTGC
Cuts at: 0 600 2928
Size: 600 2328
BstEII G'GTnAC_C
Cuts at: 0 1611 2928
Size: 1611 1317
BstXI CCAn_nnnn'nTGG
Cuts at: 0 945 1425 2928
Size: 945 480 1503
Fragments arranged by size:
1503 945 480
BstYI r'GATC_y
Cuts at: 0 378 1163 1280 1328 1593 2636 2849 2928
Size: 378 785 117 48 265 1043 213 79
Fragments arranged by size:
1043 785 378 265 213 117 79 48
Bsu36I CC'TnA_GG
Cuts at: 0 253 2928
Size: 253 2675
BtgI C'CryG_G
Cuts at: 0 370 851 1754 1767 2561 2928
Size: 370 481 903 13 794 367
Fragments arranged by size:
903 794 481 370 367 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2136 2928
Size: 2136 792
BtsI GCAGTG_nn'
Cuts at: 0 547 2928
Size: 547 2381
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1464 1499 2928
Size: 1464 35 1429
Fragments arranged by size:
1464 1429 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1210 2928
Size: 1210 1718
EaeI y'GGCC_r
Cuts at: 0 587 656 795 854 2515 2840 2866 2928
Size: 587 69 139 59 1661 325 26 62
Fragments arranged by size:
1661 587 325 139 69 62 59 26
EagI C'GGCC_G
Cuts at: 0 2515 2928
Size: 2515 413
EarI CTCTTCn'nnn_
Cuts at: 0 1081 1816 2095 2482 2793 2811 2928
Size: 1081 735 279 387 311 18 117
Fragments arranged by size:
1081 735 387 311 279 117 18
EcoICRI GAG'CTC
Cuts at: 0 903 2928
Size: 903 2025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 1354 1447 1547 1937 1996 2119 2359
Size: 1342 12 93 100 390 59 123 240
Cuts at: 2359 2650 2662 2928
Size: 291 12 266
Fragments arranged by size:
1342 390 291 266 240 123 100 93
59 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 558 621 2393 2928
Size: 558 63 1772 535
Fragments arranged by size:
1772 558 535 63
EcoO109I rG'GnC_Cy
Cuts at: 0 364 365 522 648 732 801 822 2076
Size: 364 1 157 126 84 69 21 1254
Cuts at: 2076 2181 2235 2499 2914 2928
Size: 105 54 264 415 14
Fragments arranged by size:
1254 415 364 264 157 126 105 84
69 54 21 14 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1302 2493 2928
Size: 1302 1191 435
Fragments arranged by size:
1302 1191 435
HaeII r_GCGC'y
Cuts at: 0 21 133 185 880 1296 1344 2928
Size: 21 112 52 695 416 48 1584
Fragments arranged by size:
1584 695 416 112 52 48 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 311 343 2642 2674 2928
Size: 226 32 53 32 2299 32 254
Fragments arranged by size:
2299 254 226 53 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 2709 2928
Size: 1025 1684 219
Fragments arranged by size:
1684 1025 219
MscI TGG'CCA
Cuts at: 0 658 797 856 2868 2928
Size: 658 139 59 2012 60
Fragments arranged by size:
2012 658 139 60 59
MspA1I CmG'CkG
Cuts at: 0 56 411 2928
Size: 56 355 2517
Fragments arranged by size:
2517 355 56
NaeI GCC'GGC
Cuts at: 0 2892 2928
Size: 2892 36
NcoI C'CATG_G
Cuts at: 0 851 2928
Size: 851 2077
NgoMIV G'CCGG_C
Cuts at: 0 2890 2928
Size: 2890 38
NspI r_CATG'y
Cuts at: 0 1524 2659 2928
Size: 1524 1135 269
Fragments arranged by size:
1524 1135 269
PasI CC'CwG_GG
Cuts at: 0 525 1194 2502 2928
Size: 525 669 1308 426
Fragments arranged by size:
1308 669 525 426
PflMI CCAn_nnn'nTGG
Cuts at: 0 2337 2628 2928
Size: 2337 291 300
Fragments arranged by size:
2337 300 291
PfoI T'CCnGG_A
Cuts at: 0 662 1148 1271 1331 2177 2543 2928
Size: 662 486 123 60 846 366 385
Fragments arranged by size:
846 662 486 385 366 123 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 739 771 2928
Size: 739 32 2157
Fragments arranged by size:
2157 739 32
PpuMI rG'GwC_Cy
Cuts at: 0 822 2076 2181 2235 2914 2928
Size: 822 1254 105 54 679 14
Fragments arranged by size:
1254 822 679 105 54 14
PshAI GACnn'nnGTC
Cuts at: 0 1718 2928
Size: 1718 1210
PsiI TTA'TAA
Cuts at: 0 2534 2928
Size: 2534 394
PspOMI G'GGCC_C
Cuts at: 0 364 2928
Size: 364 2564
PstI C_TGCA'G
Cuts at: 0 1461 2928
Size: 1461 1467
SacI G_AGCT'C
Cuts at: 0 905 2928
Size: 905 2023
SanDI GG'GwC_CC
Cuts at: 0 2076 2928
Size: 2076 852
SapI GCTCTTCn'nnn_
Cuts at: 0 1816 2928
Size: 1816 1112
SfcI C'TryA_G
Cuts at: 0 238 1457 2928
Size: 238 1219 1471
Fragments arranged by size:
1471 1219 238
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 655 794 1035 2928
Size: 655 139 241 1893
Fragments arranged by size:
1893 655 241 139
SgrAI Cr'CCGG_yG
Cuts at: 0 982 2928
Size: 982 1946
SmaI CCC'GGG
Cuts at: 0 2912 2928
Size: 2912 16
SmlI C'TyrA_G
Cuts at: 0 694 706 904 2928
Size: 694 12 198 2024
Fragments arranged by size:
2024 694 198 12
StuI AGG'CCT
Cuts at: 0 611 2388 2928
Size: 611 1777 540
Fragments arranged by size:
1777 611 540
StyI C'CwwG_G
Cuts at: 0 644 767 806 851 974 1829 2210 2394
Size: 644 123 39 45 123 855 381 184
Cuts at: 2394 2714 2928
Size: 320 214
Fragments arranged by size:
855 644 381 320 214 184 123 123
45 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 431 1493 2928
Size: 431 1062 1435
Fragments arranged by size:
1435 1062 431
TatI w'GTAC_w
Cuts at: 0 866 2873 2928
Size: 866 2007 55
Fragments arranged by size:
2007 866 55
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 651 2928
Size: 651 2277
XmaI C'CCGG_G
Cuts at: 0 2910 2928
Size: 2910 18
ZraI GAC'GTC
Cuts at: 0 27 2928
Size: 27 2901
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI
MscI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PfoI PpiI PpuMI PshAI PsiI PspOMI PstI SacI
SanDI SapI SfcI SfiI SgrAI SmaI SmlI StuI
StyI TaqII TatI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AhdI AleI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BfrBI BmtI BspHI
BsrBI BssHII BstBI BstZ17I ClaI DraI DraIII EciI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MslI NarI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PspXI
PsrI PvuI PvuII RsrII SacII SalI SbfI ScaI
SexAI SfoI SgfI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmnI