Restriction map for
pF1KB0458
(Linear) (Six-base) MAPSORT of: KIBB0458.seq Check: 7271 from: 1 to: 2847
KIBB0458.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 898 2847
Size: 898 1949
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 869 1924 1963 2653 2847
Size: 809 60 1055 39 690 194
Fragments arranged by size:
1055 809 690 194 60 39
AflIII A'CryG_T
Cuts at: 0 158 1336 2847
Size: 158 1178 1511
Fragments arranged by size:
1511 1178 158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 252 2388 2847
Size: 252 2136 459
Fragments arranged by size:
2136 459 252
AlwNI CAG_nnn'CTG
Cuts at: 0 691 2847
Size: 691 2156
ApaLI G'TGCA_C
Cuts at: 0 2581 2847
Size: 2581 266
ApoI r'AATT_y
Cuts at: 0 879 897 1027 1201 1716 2494 2665 2847
Size: 879 18 130 174 515 778 171 182
Fragments arranged by size:
879 778 515 182 174 171 130 18
AvaI C'yCGr_G
Cuts at: 0 300 1122 1516 2048 2847
Size: 300 822 394 532 799
Fragments arranged by size:
822 799 532 394 300
AvrII C'CTAG_G
Cuts at: 0 549 2847
Size: 549 2298
BamHI G'GATC_C
Cuts at: 0 2246 2847
Size: 2246 601
BanI G'GyrC_C
Cuts at: 0 384 1749 1858 2847
Size: 384 1365 109 989
Fragments arranged by size:
1365 989 384 109
BanII G_rGCy'C
Cuts at: 0 1238 1478 2153 2236 2594 2847
Size: 1238 240 675 83 358 253
Fragments arranged by size:
1238 675 358 253 240 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 2641 2847
Size: 2641 206
BfrBI ATG'CAT
Cuts at: 0 2283 2847
Size: 2283 564
BglII A'GATC_T
Cuts at: 0 918 1781 2847
Size: 918 863 1066
Fragments arranged by size:
1066 918 863
Bme1580I G_kGCm'C
Cuts at: 0 1754 2585 2847
Size: 1754 831 262
Fragments arranged by size:
1754 831 262
BmgBI CAC'GTC
Cuts at: 0 159 2007 2016 2847
Size: 159 1848 9 831
Fragments arranged by size:
1848 831 159 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 1677 2796 2811 2847
Size: 1677 1119 15 36
Fragments arranged by size:
1677 1119 36 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 63 2847
Size: 63 2784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2578 2847
Size: 2578 269
Bpu10I CC'TnA_GC
Cuts at: 0 1477 2847
Size: 1477 1370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1252 2470 2847
Size: 1252 1218 377
Fragments arranged by size:
1252 1218 377
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 407 2246 2847
Size: 46 361 1839 601
Fragments arranged by size:
1839 601 361 46
BsaAI yAC'GTr
Cuts at: 0 1612 2847
Size: 1612 1235
BsaHI Gr'CG_yC
Cuts at: 0 1389 2847
Size: 1389 1458
BsaWI w'CCGG_w
Cuts at: 0 444 1968 2847
Size: 444 1524 879
Fragments arranged by size:
1524 879 444
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1288 1318 2119 2149 2847
Size: 1288 30 801 30 698
Fragments arranged by size:
1288 801 698 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 36 1185 2602 2847
Size: 36 1149 1417 245
Fragments arranged by size:
1417 1149 245 36
BseYI C'CCAG_C
Cuts at: 0 567 1588 1863 2847
Size: 567 1021 275 984
Fragments arranged by size:
1021 984 567 275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1588 2847
Size: 454 1134 1259
Fragments arranged by size:
1259 1134 454
BsiHKAI G_wGCw'C
Cuts at: 0 1238 1986 2236 2585 2594 2743 2847
Size: 1238 748 250 349 9 149 104
Fragments arranged by size:
1238 748 349 250 149 104 9
BsmI GAATG_Cn'
Cuts at: 0 1445 2847
Size: 1445 1402
Bsp1286I G_dGCh'C
Cuts at: 0 1238 1478 1754 1986 2153 2236 2585 2594
Size: 1238 240 276 232 167 83 349 9
Cuts at: 2594 2743 2847
Size: 149 104
Fragments arranged by size:
1238 349 276 240 232 167 149 104
83 9
BspEI T'CCGG_A
Cuts at: 0 444 1968 2847
Size: 444 1524 879
Fragments arranged by size:
1524 879 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 282 898 2621 2847
Size: 282 616 1723 226
Fragments arranged by size:
1723 616 282 226
BsrDI GCAATG_nn'
Cuts at: 0 777 2623 2709 2847
Size: 777 1846 86 138
Fragments arranged by size:
1846 777 138 86
BssSI C'ACGA_G
Cuts at: 0 76 1459 2152 2584 2847
Size: 76 1383 693 432 263
Fragments arranged by size:
1383 693 432 263 76
BstBI TT'CG_AA
Cuts at: 0 2171 2847
Size: 2171 676
BstXI CCAn_nnnn'nTGG
Cuts at: 0 63 816 1686 2847
Size: 63 753 870 1161
Fragments arranged by size:
1161 870 753 63
BstYI r'GATC_y
Cuts at: 0 601 717 918 1175 1781 2246 2847
Size: 601 116 201 257 606 465 601
Fragments arranged by size:
606 601 601 465 257 201 116
Bsu36I CC'TnA_GG
Cuts at: 0 366 2847
Size: 366 2481
BtgI C'CryG_G
Cuts at: 0 656 1701 2847
Size: 656 1045 1146
Fragments arranged by size:
1146 1045 656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 123 1882 2465 2823 2847
Size: 123 1759 583 358 24
Fragments arranged by size:
1759 583 358 123 24
BtsI GCAGTG_nn'
Cuts at: 0 20 149 335 2847
Size: 20 129 186 2512
Fragments arranged by size:
2512 186 129 20
ClaI AT'CG_AT
Cuts at: 0 1652 2847
Size: 1652 1195
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1028 1063 1273 1308 2847
Size: 1028 35 210 35 1539
Fragments arranged by size:
1539 1028 210 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 619 1511 1981 2847
Size: 619 892 470 866
Fragments arranged by size:
892 866 619 470
DrdI GACnn_nn'nnGTC
Cuts at: 0 163 560 2847
Size: 163 397 2287
Fragments arranged by size:
2287 397 163
EaeI y'GGCC_r
Cuts at: 0 110 1688 2847
Size: 110 1578 1159
Fragments arranged by size:
1578 1159 110
EarI CTCTTCn'nnn_
Cuts at: 0 32 1795 2847
Size: 32 1763 1052
Fragments arranged by size:
1763 1052 32
EcoICRI GAG'CTC
Cuts at: 0 1236 2234 2592 2847
Size: 1236 998 358 255
Fragments arranged by size:
1236 998 358 255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 869 1924 1963 2578 2653 2847
Size: 809 60 1055 39 615 75 194
Fragments arranged by size:
1055 809 615 194 75 60 39
EcoO109I rG'GnC_Cy
Cuts at: 0 843 1140 1581 2847
Size: 843 297 441 1266
Fragments arranged by size:
1266 843 441 297
EcoRI G'AATT_C
Cuts at: 0 2494 2665 2847
Size: 2494 171 182
Fragments arranged by size:
2494 182 171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 773 1506 2847
Size: 773 733 1341
Fragments arranged by size:
1341 773 733
FspI TGC'GCA
Cuts at: 0 2715 2847
Size: 2715 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2268 2300 2388 2420 2847
Size: 2268 32 88 32 427
Fragments arranged by size:
2268 427 88 32 32
HincII GTy'rAC
Cuts at: 0 2271 2847
Size: 2271 576
HindIII A'AGCT_T
Cuts at: 0 1453 2847
Size: 1453 1394
MfeI C'AATT_G
Cuts at: 0 1040 2847
Size: 1040 1807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 1357 1573 2847
Size: 150 1207 216 1274
Fragments arranged by size:
1274 1207 216 150
MscI TGG'CCA
Cuts at: 0 112 1690 2847
Size: 112 1578 1157
Fragments arranged by size:
1578 1157 112
MslI CAynn'nnrTG
Cuts at: 0 142 640 1363 2014 2278 2335 2847
Size: 142 498 723 651 264 57 512
Fragments arranged by size:
723 651 512 498 264 142 57
MspA1I CmG'CkG
Cuts at: 0 567 816 1189 2760 2847
Size: 567 249 373 1571 87
Fragments arranged by size:
1571 567 373 249 87
NcoI C'CATG_G
Cuts at: 0 656 2847
Size: 656 2191
NdeI CA'TA_TG
Cuts at: 0 1345 2847
Size: 1345 1502
NsiI A_TGCA'T
Cuts at: 0 2285 2847
Size: 2285 562
NspI r_CATG'y
Cuts at: 0 1119 1340 1362 2847
Size: 1119 221 22 1485
Fragments arranged by size:
1485 1119 221 22
PasI CC'CwG_GG
Cuts at: 0 577 2847
Size: 577 2270
PciI A'CATG_T
Cuts at: 0 1336 2847
Size: 1336 1511
PflMI CCAn_nnn'nTGG
Cuts at: 0 1431 2010 2243 2847
Size: 1431 579 233 604
Fragments arranged by size:
1431 604 579 233
PpuMI rG'GwC_Cy
Cuts at: 0 843 1581 2847
Size: 843 738 1266
Fragments arranged by size:
1266 843 738
PstI C_TGCA'G
Cuts at: 0 906 2847
Size: 906 1941
PvuII CAG'CTG
Cuts at: 0 567 1189 2760 2847
Size: 567 622 1571 87
Fragments arranged by size:
1571 622 567 87
SacI G_AGCT'C
Cuts at: 0 1238 2236 2594 2847
Size: 1238 998 358 253
Fragments arranged by size:
1238 998 358 253
SapI GCTCTTCn'nnn_
Cuts at: 0 1795 2847
Size: 1795 1052
SfcI C'TryA_G
Cuts at: 0 902 1071 1574 2847
Size: 902 169 503 1273
Fragments arranged by size:
1273 902 503 169
SmaI CCC'GGG
Cuts at: 0 1124 2050 2847
Size: 1124 926 797
Fragments arranged by size:
1124 926 797
SmlI C'TyrA_G
Cuts at: 0 1231 2485 2847
Size: 1231 1254 362
Fragments arranged by size:
1254 1231 362
SphI G_CATG'C
Cuts at: 0 1119 2847
Size: 1119 1728
SspI AAT'ATT
Cuts at: 0 1329 2847
Size: 1329 1518
StyI C'CwwG_G
Cuts at: 0 119 549 656 2177 2310 2847
Size: 119 430 107 1521 133 537
Fragments arranged by size:
1521 537 430 133 119 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 559 1696 2506 2847
Size: 559 1137 810 341
Fragments arranged by size:
1137 810 559 341
TatI w'GTAC_w
Cuts at: 0 1320 2117 2829 2847
Size: 1320 797 712 18
Fragments arranged by size:
1320 797 712 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 745 777 1497 1529 2119 2151 2803 2835
Size: 745 32 720 32 590 32 652 32
Cuts at: 2835 2847
Size: 12
Fragments arranged by size:
745 720 652 590 32 32 32 32
12
Tth111I GACn'n_nGTC
Cuts at: 0 2005 2847
Size: 2005 842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 2427 2847
Size: 126 2301 420
Fragments arranged by size:
2301 420 126
XmaI C'CCGG_G
Cuts at: 0 1122 2048 2847
Size: 1122 926 799
Fragments arranged by size:
1122 926 799
XmnI GAAnn'nnTTC
Cuts at: 0 1736 1813 2847
Size: 1736 77 1034
Fragments arranged by size:
1736 1034 77
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaLI ApoI AvaI
AvrII BamHI BanI BanII BbsI BfrBI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrDI BssSI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PasI PciI PflMI PpuMI
PstI PvuII SacI SapI SfcI SmaI SmlI SphI
SspI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI BaeI BbeI
BbvCI BcgI BciVI BclI BglI BlpI BmtI BsaBI
BsiEI BsiWI BsmBI BspHI BsrBI BsrFI BsrGI BssHII
BstAPI BstEII BstZ17I DraI EagI EciI EcoNI EcoRV
FseI FspAI HaeII HpaI KasI KpnI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI
XbaI XhoI ZraI