Restriction map for
pF1KB0455
(Linear) (Six-base) MAPSORT of: KIBB0455.seq Check: 9956 from: 1 to: 1515
KIBB0455.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1384 1515
Size: 1384 131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 259 283 832 991 1055 1515
Size: 97 162 24 549 159 64 460
Fragments arranged by size:
549 460 162 159 97 64 24
AflIII A'CryG_T
Cuts at: 0 1016 1250 1515
Size: 1016 234 265
Fragments arranged by size:
1016 265 234
AhdI GACnn_n'nnGTC
Cuts at: 0 1254 1515
Size: 1254 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 431 1515
Size: 431 1084
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 355 387 813 845 1515
Size: 355 32 426 32 670
Fragments arranged by size:
670 426 355 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 237 261 600 645 1515
Size: 237 24 339 45 870
Fragments arranged by size:
870 339 237 45 24
ApaI G_GGCC'C
Cuts at: 0 690 1515
Size: 690 825
ApoI r'AATT_y
Cuts at: 0 1062 1515
Size: 1062 453
AvaI C'yCGr_G
Cuts at: 0 341 739 1324 1384 1429 1515
Size: 341 398 585 60 45 86
Fragments arranged by size:
585 398 341 86 60 45
BanI G'GyrC_C
Cuts at: 0 472 1299 1515
Size: 472 827 216
Fragments arranged by size:
827 472 216
BanII G_rGCy'C
Cuts at: 0 73 690 920 1515
Size: 73 617 230 595
Fragments arranged by size:
617 595 230 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 120 179 979 1224 1515
Size: 120 59 800 245 291
Fragments arranged by size:
800 291 245 120 59
BclI T'GATC_A
Cuts at: 0 518 1515
Size: 518 997
Bme1580I G_kGCm'C
Cuts at: 0 326 690 1515
Size: 326 364 825
Fragments arranged by size:
825 364 326
BmgBI CAC'GTC
Cuts at: 0 1251 1515
Size: 1251 264
BmrI ACTGGGnnnn_n'
Cuts at: 0 320 1515
Size: 320 1195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 577 815 1363 1489 1515
Size: 293 284 238 548 126 26
Fragments arranged by size:
548 293 284 238 126 26
BsaHI Gr'CG_yC
Cuts at: 0 101 1400 1515
Size: 101 1299 115
Fragments arranged by size:
1299 115 101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 813 843 1515
Size: 550 30 233 30 672
Fragments arranged by size:
672 550 233 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 134 1447 1515
Size: 134 1313 68
Fragments arranged by size:
1313 134 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1515
Size: 625 890
Bsp1286I G_dGCh'C
Cuts at: 0 73 326 690 920 1515
Size: 73 253 364 230 595
Fragments arranged by size:
595 364 253 230 73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 1088 1384 1515
Size: 236 852 296 131
Fragments arranged by size:
852 296 236 131
BsrBI CCG'CTC
Cuts at: 0 882 1437 1494 1515
Size: 882 555 57 21
Fragments arranged by size:
882 555 57 21
BsrFI r'CCGG_y
Cuts at: 0 433 1424 1515
Size: 433 991 91
Fragments arranged by size:
991 433 91
BssSI C'ACGA_G
Cuts at: 0 877 1515
Size: 877 638
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1464 1515
Size: 1464 51
BstEII G'GTnAC_C
Cuts at: 0 906 1515
Size: 906 609
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1098 1515
Size: 1098 417
BstYI r'GATC_y
Cuts at: 0 533 704 1515
Size: 533 171 811
Fragments arranged by size:
811 533 171
BtgI C'CryG_G
Cuts at: 0 1395 1515
Size: 1395 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1389 1515
Size: 1389 126
BtsI GCAGTG_nn'
Cuts at: 0 109 908 1515
Size: 109 799 607
Fragments arranged by size:
799 607 109
EaeI y'GGCC_r
Cuts at: 0 287 797 944 1088 1484 1515
Size: 287 510 147 144 396 31
Fragments arranged by size:
510 396 287 147 144 31
EarI CTCTTCn'nnn_
Cuts at: 0 303 1515
Size: 303 1212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 259 283 293 577 815 832 991
Size: 97 162 24 10 284 238 17 159
Cuts at: 991 1055 1363 1489 1515
Size: 64 308 126 26
Fragments arranged by size:
308 284 238 162 159 126 97 64
26 24 17 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 847 1515
Size: 847 668
EcoO109I rG'GnC_Cy
Cuts at: 0 225 258 597 1515
Size: 225 33 339 918
Fragments arranged by size:
918 339 225 33
EcoRI G'AATT_C
Cuts at: 0 1062 1515
Size: 1062 453
HaeII r_GCGC'y
Cuts at: 0 89 1515
Size: 89 1426
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 852 884 983 1015 1240 1272 1515
Size: 852 32 99 32 225 32 243
Fragments arranged by size:
852 243 225 99 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 915 1515
Size: 370 545 600
Fragments arranged by size:
600 545 370
MscI TGG'CCA
Cuts at: 0 289 799 946 1515
Size: 289 510 147 569
Fragments arranged by size:
569 510 289 147
MslI CAynn'nnrTG
Cuts at: 0 167 211 793 1099 1515
Size: 167 44 582 306 416
Fragments arranged by size:
582 416 306 167 44
MspA1I CmG'CkG
Cuts at: 0 539 1397 1515
Size: 539 858 118
Fragments arranged by size:
858 539 118
NaeI GCC'GGC
Cuts at: 0 435 1426 1515
Size: 435 991 89
Fragments arranged by size:
991 435 89
NgoMIV G'CCGG_C
Cuts at: 0 433 1424 1515
Size: 433 991 91
Fragments arranged by size:
991 433 91
NspI r_CATG'y
Cuts at: 0 1020 1362 1515
Size: 1020 342 153
Fragments arranged by size:
1020 342 153
PasI CC'CwG_GG
Cuts at: 0 507 683 1309 1515
Size: 507 176 626 206
Fragments arranged by size:
626 507 206 176
PciI A'CATG_T
Cuts at: 0 1016 1515
Size: 1016 499
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 1199 1515
Size: 600 599 316
Fragments arranged by size:
600 599 316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 813 845 1515
Size: 813 32 670
Fragments arranged by size:
813 670 32
PpuMI rG'GwC_Cy
Cuts at: 0 225 258 1515
Size: 225 33 1257
Fragments arranged by size:
1257 225 33
PspOMI G'GGCC_C
Cuts at: 0 686 1515
Size: 686 829
PstI C_TGCA'G
Cuts at: 0 233 875 1460 1511 1515
Size: 233 642 585 51 4
Fragments arranged by size:
642 585 233 51 4
SacII CC_GC'GG
Cuts at: 0 1398 1515
Size: 1398 117
SfcI C'TryA_G
Cuts at: 0 229 253 871 1456 1507 1515
Size: 229 24 618 585 51 8
Fragments arranged by size:
618 585 229 51 24 8
SphI G_CATG'C
Cuts at: 0 1362 1515
Size: 1362 153
StuI AGG'CCT
Cuts at: 0 1111 1515
Size: 1111 404
StyI C'CwwG_G
Cuts at: 0 911 1003 1515
Size: 911 92 512
Fragments arranged by size:
911 512 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 520 835 1515
Size: 520 315 680
Fragments arranged by size:
680 520 315
TatI w'GTAC_w
Cuts at: 0 938 1515
Size: 938 577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 1515
Size: 333 1182
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AloI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BclI Bme1580I BmgBI
BmrI BpmI BsaHI BsaXI BseRI BsgI Bsp1286I BspMI
BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PasI PciI PflMI PpiI PpuMI PspOMI PstI
SacII SfcI SphI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI