Restriction map for
pF1KB0453
(Linear) (Six-base) MAPSORT of: KIBB0453.seq Check: 9140 from: 1 to: 1515
KIBB0453.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 0 716 1515
Size: 716 799
Acc65I G'GTAC_C
Cuts at: 0 1028 1515
Size: 1028 487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1515
Size: 592 923
AflIII A'CryG_T
Cuts at: 0 158 1515
Size: 158 1357
AleI CACnn'nnGTG
Cuts at: 0 208 1515
Size: 208 1307
AlwNI CAG_nnn'CTG
Cuts at: 0 1104 1116 1515
Size: 1104 12 399
Fragments arranged by size:
1104 399 12
ApoI r'AATT_y
Cuts at: 0 438 859 1515
Size: 438 421 656
Fragments arranged by size:
656 438 421
AvaI C'yCGr_G
Cuts at: 0 143 398 940 1515
Size: 143 255 542 575
Fragments arranged by size:
575 542 255 143
BanI G'GyrC_C
Cuts at: 0 945 1028 1515
Size: 945 83 487
Fragments arranged by size:
945 487 83
BbvCI CC'TCA_GC
Cuts at: 0 1474 1515
Size: 1474 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1140 1174 1515
Size: 1140 34 341
Fragments arranged by size:
1140 341 34
Bme1580I G_kGCm'C
Cuts at: 0 950 1491 1515
Size: 950 541 24
Fragments arranged by size:
950 541 24
BmgBI CAC'GTC
Cuts at: 0 738 1515
Size: 738 777
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1262 1515
Size: 1262 253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 886 1138 1515
Size: 526 360 252 377
Fragments arranged by size:
526 377 360 252
Bpu10I CC'TnA_GC
Cuts at: 0 1474 1515
Size: 1474 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1257 1450 1515
Size: 1257 193 65
Fragments arranged by size:
1257 193 65
BsaAI yAC'GTr
Cuts at: 0 159 1515
Size: 159 1356
BsaHI Gr'CG_yC
Cuts at: 0 1431 1515
Size: 1431 84
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 301 370 420 1515
Size: 301 69 50 1095
Fragments arranged by size:
1095 301 69 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1515
Size: 1129 386
BsiHKAI G_wGCw'C
Cuts at: 0 318 1515
Size: 318 1197
BsmI GAATG_Cn'
Cuts at: 0 589 1515
Size: 589 926
Bsp1286I G_dGCh'C
Cuts at: 0 318 950 1491 1515
Size: 318 632 541 24
Fragments arranged by size:
632 541 318 24
BspHI T'CATG_A
Cuts at: 0 725 1515
Size: 725 790
BsrDI GCAATG_nn'
Cuts at: 0 488 1158 1515
Size: 488 670 357
Fragments arranged by size:
670 488 357
BsrFI r'CCGG_y
Cuts at: 0 764 1457 1515
Size: 764 693 58
Fragments arranged by size:
764 693 58
BstBI TT'CG_AA
Cuts at: 0 63 1417 1515
Size: 63 1354 98
Fragments arranged by size:
1354 98 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 504 1515
Size: 504 1011
BstYI r'GATC_y
Cuts at: 0 299 633 1515
Size: 299 334 882
Fragments arranged by size:
882 334 299
BtsI GCAGTG_nn'
Cuts at: 0 964 1515
Size: 964 551
ClaI AT'CG_AT
Cuts at: 0 1074 1515
Size: 1074 441
EarI CTCTTCn'nnn_
Cuts at: 0 568 907 992 1031 1515
Size: 568 339 85 39 484
Fragments arranged by size:
568 484 339 85 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 592 886 1138 1515
Size: 526 66 294 252 377
Fragments arranged by size:
526 377 294 252 66
EcoO109I rG'GnC_Cy
Cuts at: 0 1101 1515
Size: 1101 414
EcoRI G'AATT_C
Cuts at: 0 438 1515
Size: 438 1077
EcoRV GAT'ATC
Cuts at: 0 1078 1515
Size: 1078 437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 471 1515
Size: 471 1044
HaeII r_GCGC'y
Cuts at: 0 328 1515
Size: 328 1187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 662 694 1232 1264 1515
Size: 662 32 538 32 251
Fragments arranged by size:
662 538 251 32 32
HincII GTy'rAC
Cuts at: 0 681 1515
Size: 681 834
KpnI G_GTAC'C
Cuts at: 0 1032 1515
Size: 1032 483
MslI CAynn'nnrTG
Cuts at: 0 208 281 649 1515
Size: 208 73 368 866
Fragments arranged by size:
866 368 208 73
MspA1I CmG'CkG
Cuts at: 0 1113 1515
Size: 1113 402
NaeI GCC'GGC
Cuts at: 0 766 1459 1515
Size: 766 693 56
Fragments arranged by size:
766 693 56
NgoMIV G'CCGG_C
Cuts at: 0 764 1457 1515
Size: 764 693 58
Fragments arranged by size:
764 693 58
NspI r_CATG'y
Cuts at: 0 1500 1515
Size: 1500 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 1104 1515
Size: 1104 411
PfoI T'CCnGG_A
Cuts at: 0 689 1515
Size: 689 826
PpuMI rG'GwC_Cy
Cuts at: 0 1101 1515
Size: 1101 414
SexAI A'CCwGG_T
Cuts at: 0 1103 1515
Size: 1103 412
SmaI CCC'GGG
Cuts at: 0 145 400 1515
Size: 145 255 1115
Fragments arranged by size:
1115 255 145
SmlI C'TyrA_G
Cuts at: 0 1236 1465 1515
Size: 1236 229 50
Fragments arranged by size:
1236 229 50
SspI AAT'ATT
Cuts at: 0 501 1515
Size: 501 1014
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1274 1515
Size: 1274 241
XmaI C'CCGG_G
Cuts at: 0 143 398 1515
Size: 143 255 1117
Fragments arranged by size:
1117 255 143
XmnI GAAnn'nnTTC
Cuts at: 0 442 1515
Size: 442 1073
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlwNI ApoI AvaI
BanI BbvCI BcgI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BsrFI BstBI BstXI BstYI BtsI ClaI
EarI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII KpnI MslI MspA1I NaeI NgoMIV NspI PflMI
PfoI PpuMI SexAI SmaI SmlI SspI TaqII XmaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI BmrI BmtI BsaI BsaBI BsaWI BsaXI
BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI FseI FspI FspAI HindIII HpaI KasI MfeI
MluI MmeI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI StuI StyI SwaI TatI TstI Tth111I XbaI
XcmI XhoI ZraI