Restriction map for
pF1KA1930
(Linear) (Six-base) MAPSORT of: KIAA1930.seq Check: 4976 from: 1 to: 3657
KIAA1930.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 729 3657
Size: 729 2928
AccI GT'mk_AC
Cuts at: 0 2239 3657
Size: 2239 1418
Acc65I G'GTAC_C
Cuts at: 0 916 3657
Size: 916 2741
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 262 832 1127 1373 1598 2532 3256
Size: 102 160 570 295 246 225 934 724
Cuts at: 3256 3657
Size: 401
Fragments arranged by size:
934 724 570 401 295 246 225 160
102
AfeI AGC'GCT
Cuts at: 0 700 1018 3657
Size: 700 318 2639
Fragments arranged by size:
2639 700 318
AleI CACnn'nnGTG
Cuts at: 0 1352 3657
Size: 1352 2305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 592 3657
Size: 592 3065
AlwNI CAG_nnn'CTG
Cuts at: 0 1177 1540 1561 1606 2101 2439 3069 3094
Size: 1177 363 21 45 495 338 630 25
Cuts at: 3094 3523 3657
Size: 429 134
Fragments arranged by size:
1177 630 495 429 363 338 134 45
25 21
ApaI G_GGCC'C
Cuts at: 0 101 134 1277 2388 2753 2778 3291 3370
Size: 101 33 1143 1111 365 25 513 79
Cuts at: 3370 3390 3657
Size: 20 267
Fragments arranged by size:
1143 1111 513 365 267 101 79 33
25 20
ApaLI G'TGCA_C
Cuts at: 0 271 2919 3657
Size: 271 2648 738
Fragments arranged by size:
2648 738 271
ApoI r'AATT_y
Cuts at: 0 324 784 3657
Size: 324 460 2873
Fragments arranged by size:
2873 460 324
AvaI C'yCGr_G
Cuts at: 0 44 158 211 501 518 542 1219 2801
Size: 44 114 53 290 17 24 677 1582
Cuts at: 2801 3256 3434 3475 3657
Size: 455 178 41 182
Fragments arranged by size:
1582 677 455 290 182 178 114 53
44 41 24 17
AvrII C'CTAG_G
Cuts at: 0 1198 3456 3657
Size: 1198 2258 201
Fragments arranged by size:
2258 1198 201
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 900 933 3657
Size: 900 33 2724
Fragments arranged by size:
2724 900 33
BanI G'GyrC_C
Cuts at: 0 201 463 916 2702 2989 3252 3657
Size: 201 262 453 1786 287 263 405
Fragments arranged by size:
1786 453 405 287 263 262 201
BanII G_rGCy'C
Cuts at: 0 101 134 952 1042 1226 1277 2388 2753
Size: 101 33 818 90 184 51 1111 365
Cuts at: 2753 2778 3291 3370 3390 3491 3657
Size: 25 513 79 20 101 166
Fragments arranged by size:
1111 818 513 365 184 166 101 101
90 79 51 33 25 20
BbeI G_GCGC'C
Cuts at: 0 3256 3657
Size: 3256 401
BbsI GAAGACnn'nnnn_
Cuts at: 0 108 2570 2582 3105 3657
Size: 108 2462 12 523 552
Fragments arranged by size:
2462 552 523 108 12
BbvCI CC'TCA_GC
Cuts at: 0 975 1382 2627 2662 3023 3182 3292 3657
Size: 975 407 1245 35 361 159 110 365
Fragments arranged by size:
1245 975 407 365 361 159 110 35
BclI T'GATC_A
Cuts at: 0 3219 3657
Size: 3219 438
BglI GCCn_nnn'nGGC
Cuts at: 0 216 2392 3014 3078 3261 3657
Size: 216 2176 622 64 183 396
Fragments arranged by size:
2176 622 396 216 183 64
BglII A'GATC_T
Cuts at: 0 674 3657
Size: 674 2983
BlpI GC'TnA_GC
Cuts at: 0 37 928 3657
Size: 37 891 2729
Fragments arranged by size:
2729 891 37
Bme1580I G_kGCm'C
Cuts at: 0 101 134 275 1277 2388 2655 2743 2753
Size: 101 33 141 1002 1111 267 88 10
Cuts at: 2753 2778 2923 2994 3291 3370 3390 3657
Size: 25 145 71 297 79 20 267
Fragments arranged by size:
1111 1002 297 267 267 145 141 101
88 79 71 33 25 20 10
BmgBI CAC'GTC
Cuts at: 0 3426 3657
Size: 3426 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 503 1297 1466 1774 1928 2119 2323 2389
Size: 503 794 169 308 154 191 204 66
Cuts at: 2389 2642 2746 3373 3657
Size: 253 104 627 284
Fragments arranged by size:
794 627 503 308 284 253 204 191
169 154 104 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3121 3657
Size: 3121 536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 415 601 613 1126 2046 2389 2461
Size: 280 135 186 12 513 920 343 72
Cuts at: 2461 2493 2800 2830 3067 3116 3144 3433 3613
Size: 32 307 30 237 49 28 289 180
Cuts at: 3613 3657
Size: 44
Fragments arranged by size:
920 513 343 307 289 280 237 186
180 135 72 49 44 32 30 28
12
Bpu10I CC'TnA_GC
Cuts at: 0 975 1382 2426 2627 2662 3023 3182 3292
Size: 975 407 1044 201 35 361 159 110
Cuts at: 3292 3657
Size: 365
Fragments arranged by size:
1044 975 407 365 361 201 159 110
35
BsaI GGTCTCn'nnnn_
Cuts at: 0 850 1252 3169 3657
Size: 850 402 1917 488
Fragments arranged by size:
1917 850 488 402
BsaAI yAC'GTr
Cuts at: 0 3168 3657
Size: 3168 489
BsaHI Gr'CG_yC
Cuts at: 0 74 450 693 3253 3264 3608 3657
Size: 74 376 243 2560 11 344 49
Fragments arranged by size:
2560 376 344 243 74 49 11
BsaWI w'CCGG_w
Cuts at: 0 1044 3350 3657
Size: 1044 2306 307
Fragments arranged by size:
2306 1044 307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 556 586 985 1015 3657
Size: 556 30 399 30 2642
Fragments arranged by size:
2642 556 399 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1114 1300 2386 3199 3610 3657
Size: 1114 186 1086 813 411 47
Fragments arranged by size:
1114 1086 813 411 186 47
BseYI C'CCAG_C
Cuts at: 0 189 603 1264 1446 2288 2620 3083 3344
Size: 189 414 661 182 842 332 463 261
Cuts at: 3344 3657
Size: 313
Fragments arranged by size:
842 661 463 414 332 313 261 189
182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1231 1556 2267 2893 3448 3657
Size: 1231 325 711 626 555 209
Fragments arranged by size:
1231 711 626 555 325 209
BsiEI CG_ry'CG
Cuts at: 0 22 3657
Size: 22 3635
BsiHKAI G_wGCw'C
Cuts at: 0 275 1469 1686 2923 3657
Size: 275 1194 217 1237 734
Fragments arranged by size:
1237 1194 734 275 217
BsmI GAATG_Cn'
Cuts at: 0 2292 3652 3657
Size: 2292 1360 5
Fragments arranged by size:
2292 1360 5
BsmBI CGTCTCn'nnnn_
Cuts at: 0 457 3657
Size: 457 3200
Bsp1286I G_dGCh'C
Cuts at: 0 101 134 275 952 1042 1226 1277 1469
Size: 101 33 141 677 90 184 51 192
Cuts at: 1469 1686 2388 2655 2743 2753 2778 2923 2994
Size: 217 702 267 88 10 25 145 71
Cuts at: 2994 3291 3370 3390 3491 3657
Size: 297 79 20 101 166
Fragments arranged by size:
702 677 297 267 217 192 184 166
145 141 101 101 90 88 79 71
51 33 25 20 10
BspEI T'CCGG_A
Cuts at: 0 3350 3657
Size: 3350 307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 729 3657
Size: 729 2928
BsrBI CCG'CTC
Cuts at: 0 93 216 2784 2958 3657
Size: 93 123 2568 174 699
Fragments arranged by size:
2568 699 174 123 93
BsrGI T'GTAC_A
Cuts at: 0 230 3657
Size: 230 3427
BssSI C'ACGA_G
Cuts at: 0 88 3657
Size: 88 3569
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3069 3657
Size: 3069 588
BstEII G'GTnAC_C
Cuts at: 0 2451 3657
Size: 2451 1206
BstXI CCAn_nnnn'nTGG
Cuts at: 0 831 1941 3657
Size: 831 1110 1716
Fragments arranged by size:
1716 1110 831
BstYI r'GATC_y
Cuts at: 0 674 2226 2843 3657
Size: 674 1552 617 814
Fragments arranged by size:
1552 814 674 617
BtgI C'CryG_G
Cuts at: 0 136 179 1295 1458 2312 2412 3155 3309
Size: 136 43 1116 163 854 100 743 154
Cuts at: 3309 3657
Size: 348
Fragments arranged by size:
1116 854 743 348 163 154 136 100
43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 535 3657
Size: 535 3122
BtsI GCAGTG_nn'
Cuts at: 0 849 1175 2712 3562 3657
Size: 849 326 1537 850 95
Fragments arranged by size:
1537 850 849 326 95
ClaI AT'CG_AT
Cuts at: 0 416 3657
Size: 416 3241
DraIII CAC_nnn'GTG
Cuts at: 0 2650 2672 3657
Size: 2650 22 985
Fragments arranged by size:
2650 985 22
DrdI GACnn_nn'nnGTC
Cuts at: 0 361 1122 3657
Size: 361 761 2535
Fragments arranged by size:
2535 761 361
EaeI y'GGCC_r
Cuts at: 0 19 821 1500 2309 2415 2849 3641 3657
Size: 19 802 679 809 106 434 792 16
Fragments arranged by size:
809 802 792 679 434 106 19 16
EagI C'GGCC_G
Cuts at: 0 19 3657
Size: 19 3638
EarI CTCTTCn'nnn_
Cuts at: 0 3560 3657
Size: 3560 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 262 280 415 601 613 832 1126
Size: 102 160 18 135 186 12 219 294
Cuts at: 1126 1127 1373 1598 2046 2389 2461 2493 2532
Size: 1 246 225 448 343 72 32 39
Cuts at: 2532 2800 2830 3067 3116 3144 3256 3433 3613
Size: 268 30 237 49 28 112 177 180
Cuts at: 3613 3657
Size: 44
Fragments arranged by size:
448 343 294 268 246 237 225 219
186 180 177 160 135 112 102 72
49 44 39 32 30 28 18 12
1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 532 883 1248 2371 2443 3657
Size: 532 351 365 1123 72 1214
Fragments arranged by size:
1214 1123 532 365 351 72
EcoO109I rG'GnC_Cy
Cuts at: 0 97 130 131 249 497 816 864 1202
Size: 97 33 1 118 248 319 48 338
Cuts at: 1202 1274 1426 1627 1756 2384 2385 2436 2750
Size: 72 152 201 129 628 1 51 314
Cuts at: 2750 2775 3080 3091 3240 3288 3367 3386 3387
Size: 25 305 11 149 48 79 19 1
Cuts at: 3387 3657
Size: 270
Fragments arranged by size:
628 338 319 314 305 270 248 201
152 149 129 118 97 79 72 51
48 48 33 25 19 11 1 1
1
EcoRI G'AATT_C
Cuts at: 0 324 3657
Size: 324 3333
EcoRV GAT'ATC
Cuts at: 0 903 3657
Size: 903 2754
HaeII r_GCGC'y
Cuts at: 0 30 44 241 702 1020 3256 3657
Size: 30 14 197 461 318 2236 401
Fragments arranged by size:
2236 461 401 318 197 30 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 558 590 958 990 1039 1071 1138 1170
Size: 558 32 368 32 49 32 67 32
Cuts at: 1170 2472 2504 3559 3591 3657
Size: 1302 32 1055 32 66
Fragments arranged by size:
1302 1055 558 368 67 66 49 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 993 3657
Size: 993 2664
KasI G'GCGC_C
Cuts at: 0 3252 3657
Size: 3252 405
KpnI G_GTAC'C
Cuts at: 0 920 3657
Size: 920 2737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1315 3657
Size: 1315 2342
MscI TGG'CCA
Cuts at: 0 823 1502 2311 2417 3643 3657
Size: 823 679 809 106 1226 14
Fragments arranged by size:
1226 823 809 679 106 14
MslI CAynn'nnrTG
Cuts at: 0 1352 2524 3657
Size: 1352 1172 1133
Fragments arranged by size:
1352 1172 1133
MspA1I CmG'CkG
Cuts at: 0 447 603 979 2936 3181 3411 3657
Size: 447 156 376 1957 245 230 246
Fragments arranged by size:
1957 447 376 246 245 230 156
NarI GG'CG_CC
Cuts at: 0 3253 3657
Size: 3253 404
NcoI C'CATG_G
Cuts at: 0 1458 2312 3155 3309 3657
Size: 1458 854 843 154 348
Fragments arranged by size:
1458 854 843 348 154
NotI GC'GGCC_GC
Cuts at: 0 19 3657
Size: 19 3638
NruI TCG'CGA
Cuts at: 0 554 3657
Size: 554 3103
NspI r_CATG'y
Cuts at: 0 573 1086 1324 2536 3657
Size: 573 513 238 1212 1121
Fragments arranged by size:
1212 1121 573 513 238
PasI CC'CwG_GG
Cuts at: 0 3057 3657
Size: 3057 600
PflMI CCAn_nnn'nTGG
Cuts at: 0 891 2868 3094 3657
Size: 891 1977 226 563
Fragments arranged by size:
1977 891 563 226
PfoI T'CCnGG_A
Cuts at: 0 166 455 3044 3377 3401 3657
Size: 166 289 2589 333 24 256
Fragments arranged by size:
2589 333 289 256 166 24
PpuMI rG'GwC_Cy
Cuts at: 0 497 864 1426 3657
Size: 497 367 562 2231
Fragments arranged by size:
2231 562 497 367
PshAI GACnn'nnGTC
Cuts at: 0 2329 3657
Size: 2329 1328
PsiI TTA'TAA
Cuts at: 0 2020 3657
Size: 2020 1637
PspOMI G'GGCC_C
Cuts at: 0 97 130 1273 2384 2749 2774 3287 3366
Size: 97 33 1143 1111 365 25 513 79
Cuts at: 3366 3386 3657
Size: 20 271
Fragments arranged by size:
1143 1111 513 365 271 97 79 33
25 20
PspXI vC'TCGA_Gb
Cuts at: 0 3434 3657
Size: 3434 223
PstI C_TGCA'G
Cuts at: 0 2435 2719 3657
Size: 2435 284 938
Fragments arranged by size:
2435 938 284
PvuII CAG'CTG
Cuts at: 0 603 979 2936 3411 3657
Size: 603 376 1957 475 246
Fragments arranged by size:
1957 603 475 376 246
SanDI GG'GwC_CC
Cuts at: 0 497 3657
Size: 497 3160
ScaI AGT'ACT
Cuts at: 0 2807 3657
Size: 2807 850
SexAI A'CCwGG_T
Cuts at: 0 224 3657
Size: 224 3433
SfcI C'TryA_G
Cuts at: 0 1029 1329 1553 1643 1656 1709 1751 2115
Size: 1029 300 224 90 13 53 42 364
Cuts at: 2115 2431 2715 3550 3657
Size: 316 284 835 107
Fragments arranged by size:
1029 835 364 316 300 284 224 107
90 53 42 13
SfoI GGC'GCC
Cuts at: 0 3254 3657
Size: 3254 403
SmaI CCC'GGG
Cuts at: 0 46 503 520 3657
Size: 46 457 17 3137
Fragments arranged by size:
3137 457 46 17
SmlI C'TyrA_G
Cuts at: 0 3434 3657
Size: 3434 223
SphI G_CATG'C
Cuts at: 0 1324 2536 3657
Size: 1324 1212 1121
Fragments arranged by size:
1324 1212 1121
StuI AGG'CCT
Cuts at: 0 1000 1254 2601 3163 3657
Size: 1000 254 1347 562 494
Fragments arranged by size:
1347 1000 562 494 254
StyI C'CwwG_G
Cuts at: 0 197 860 912 1198 1277 1376 1458 2312
Size: 197 663 52 286 79 99 82 854
Cuts at: 2312 2537 2693 3140 3155 3309 3456 3657
Size: 225 156 447 15 154 147 201
Fragments arranged by size:
854 663 447 286 225 201 197 156
154 147 99 82 79 52 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 203 1460 1471 1735 1819 1882 1903 1987
Size: 203 1257 11 264 84 63 21 84
Cuts at: 1987 2008 2192 2661 3358 3657
Size: 21 184 469 697 299
Fragments arranged by size:
1257 697 469 299 264 203 184 84
84 63 21 21 11
TatI w'GTAC_w
Cuts at: 0 230 557 2805 3657
Size: 230 327 2248 852
Fragments arranged by size:
2248 852 327 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 556 588 725 757 1908 1940 2658 2690
Size: 556 32 137 32 1151 32 718 32
Cuts at: 2690 3657
Size: 967
Fragments arranged by size:
1151 967 718 556 137 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2486 3657
Size: 2486 1171
XbaI T'CTAG_A
Cuts at: 0 2043 3657
Size: 2043 1614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3091 3657
Size: 3091 566
XhoI C'TCGA_G
Cuts at: 0 3434 3657
Size: 3434 223
XmaI C'CCGG_G
Cuts at: 0 44 501 518 3657
Size: 44 457 17 3139
Fragments arranged by size:
3139 457 44 17
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BaeI BanI BanII
BbeI BbsI BbvCI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrGI BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI NotI NruI NspI PasI
PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuII SanDI ScaI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AloI AscI
AseI AsiSI BamHI BcgI BciVI BfrBI BmtI BpuEI
BsaBI BsiWI BspHI BsrDI BsrFI BssHII BstBI BstZ17I
Bsu36I CspCI DraI EciI EcoICRI FalI FseI FspI
FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NsiI PacI PciI PmeI PmlI PpiI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XmnI
ZraI