Restriction map for
pF1KA1909
(Linear) (Six-base) MAPSORT of: KIAA1909.seq Check: 6377 from: 1 to: 3813
KIAA1909.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 555 1690 2388 3813
Size: 555 1135 698 1425
Fragments arranged by size:
1425 1135 698 555
AatII G_ACGT'C
Cuts at: 0 578 3128 3813
Size: 578 2550 685
Fragments arranged by size:
2550 685 578
AccI GT'mk_AC
Cuts at: 0 3128 3813
Size: 3128 685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 1735 2235 3813
Size: 964 771 500 1578
Fragments arranged by size:
1578 964 771 500
AfeI AGC'GCT
Cuts at: 0 1979 2614 3813
Size: 1979 635 1199
Fragments arranged by size:
1979 1199 635
AhdI GACnn_n'nnGTC
Cuts at: 0 167 2040 3813
Size: 167 1873 1773
Fragments arranged by size:
1873 1773 167
AleI CACnn'nnGTG
Cuts at: 0 3520 3813
Size: 3520 293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1883 2729 2965 3813
Size: 1883 846 236 848
Fragments arranged by size:
1883 848 846 236
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3326 3358 3813
Size: 3326 32 455
Fragments arranged by size:
3326 455 32
AlwNI CAG_nnn'CTG
Cuts at: 0 214 570 588 1044 1371 2130 2847 2889
Size: 214 356 18 456 327 759 717 42
Cuts at: 2889 3689 3710 3813
Size: 800 21 103
Fragments arranged by size:
800 759 717 456 356 327 214 103
42 21 18
ApaI G_GGCC'C
Cuts at: 0 282 1601 2014 3813
Size: 282 1319 413 1799
Fragments arranged by size:
1799 1319 413 282
ApaLI G'TGCA_C
Cuts at: 0 952 1330 3813
Size: 952 378 2483
Fragments arranged by size:
2483 952 378
ApoI r'AATT_y
Cuts at: 0 958 1092 2985 3813
Size: 958 134 1893 828
Fragments arranged by size:
1893 958 828 134
AvaI C'yCGr_G
Cuts at: 0 49 143 508 623 1693 2361 3660 3813
Size: 49 94 365 115 1070 668 1299 153
Fragments arranged by size:
1299 1070 668 365 153 115 94 49
AvrII C'CTAG_G
Cuts at: 0 339 3813
Size: 339 3474
BamHI G'GATC_C
Cuts at: 0 3302 3813
Size: 3302 511
BanI G'GyrC_C
Cuts at: 0 258 555 1228 1889 1933 3751 3813
Size: 258 297 673 661 44 1818 62
Fragments arranged by size:
1818 673 661 297 258 62 44
BanII G_rGCy'C
Cuts at: 0 282 940 1601 1852 2006 2014 2120 2326
Size: 282 658 661 251 154 8 106 206
Cuts at: 2326 2369 2900 3441 3676 3780 3813
Size: 43 531 541 235 104 33
Fragments arranged by size:
661 658 541 531 282 251 235 206
154 106 104 43 33 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 413 1199 1277 2241 3083 3813
Size: 413 786 78 964 842 730
Fragments arranged by size:
964 842 786 730 413 78
BbvCI CC'TCA_GC
Cuts at: 0 423 1246 2198 2378 3813
Size: 423 823 952 180 1435
Fragments arranged by size:
1435 952 823 423 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 472 506 3813
Size: 472 34 3307
Fragments arranged by size:
3307 472 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2467 3813
Size: 2467 1346
BglI GCCn_nnn'nGGC
Cuts at: 0 495 1876 2080 3813
Size: 495 1381 204 1733
Fragments arranged by size:
1733 1381 495 204
BglII A'GATC_T
Cuts at: 0 238 3813
Size: 238 3575
BlpI GC'TnA_GC
Cuts at: 0 701 2722 3793 3813
Size: 701 2021 1071 20
Fragments arranged by size:
2021 1071 701 20
Bme1580I G_kGCm'C
Cuts at: 0 261 282 560 956 1231 1334 1601 1872
Size: 261 21 278 396 275 103 267 271
Cuts at: 1872 2014 3493 3754 3813
Size: 142 1479 261 59
Fragments arranged by size:
1479 396 278 275 271 267 261 261
142 103 59 21
BmgBI CAC'GTC
Cuts at: 0 2982 3813
Size: 2982 831
BmrI ACTGGGnnnn_n'
Cuts at: 0 110 370 3813
Size: 110 260 3443
Fragments arranged by size:
3443 260 110
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 150 1686 2115 3813
Size: 150 1536 429 1698
Fragments arranged by size:
1698 1536 429 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 255 306 1240 1309 1348 1393 2110
Size: 98 157 51 934 69 39 45 717
Cuts at: 2110 2284 2629 2824 3630 3813
Size: 174 345 195 806 183
Fragments arranged by size:
934 806 717 345 195 183 174 157
98 69 51 45 39
Bpu10I CC'TnA_GC
Cuts at: 0 423 1246 1501 2198 2378 2469 3813
Size: 423 823 255 697 180 91 1344
Fragments arranged by size:
1344 823 697 423 255 180 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2836 3216 3813
Size: 2836 380 597
Fragments arranged by size:
2836 597 380
BsaI GGTCTCn'nnnn_
Cuts at: 0 143 620 3395 3813
Size: 143 477 2775 418
Fragments arranged by size:
2775 477 418 143
BsaAI yAC'GTr
Cuts at: 0 2682 3813
Size: 2682 1131
BsaHI Gr'CG_yC
Cuts at: 0 575 1393 2963 3125 3813
Size: 575 818 1570 162 688
Fragments arranged by size:
1570 818 688 575 162
BsaWI w'CCGG_w
Cuts at: 0 3404 3813
Size: 3404 409
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1378 1408 2030 2060 2773 2803 3813
Size: 1378 30 622 30 713 30 1010
Fragments arranged by size:
1378 1010 713 622 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 1263 1339 1614 1662 1899 2038 2395
Size: 55 1208 76 275 48 237 139 357
Cuts at: 2395 3519 3579 3600 3813
Size: 1124 60 21 213
Fragments arranged by size:
1208 1124 357 275 237 213 139 76
60 55 48 21
BseYI C'CCAG_C
Cuts at: 0 193 469 547 1825 1912 2889 3627 3651
Size: 193 276 78 1278 87 977 738 24
Cuts at: 3651 3813
Size: 162
Fragments arranged by size:
1278 977 738 276 193 162 87 78
24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 670 1548 2830 3440 3470 3813
Size: 378 292 878 1282 610 30 343
Fragments arranged by size:
1282 878 610 378 343 292 30
BsiEI CG_ry'CG
Cuts at: 0 1314 3158 3465 3813
Size: 1314 1844 307 348
Fragments arranged by size:
1844 1314 348 307
BsiHKAI G_wGCw'C
Cuts at: 0 940 956 1334 1379 2028 2900 3813
Size: 940 16 378 45 649 872 913
Fragments arranged by size:
940 913 872 649 378 45 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 816 1162 1366 1570 3739 3813
Size: 816 346 204 204 2169 74
Fragments arranged by size:
2169 816 346 204 204 74
Bsp1286I G_dGCh'C
Cuts at: 0 261 282 560 940 956 1231 1334 1379
Size: 261 21 278 380 16 275 103 45
Cuts at: 1379 1601 1852 1872 2006 2014 2028 2120 2326
Size: 222 251 20 134 8 14 92 206
Cuts at: 2326 2369 2900 3441 3493 3676 3754 3780 3813
Size: 43 531 541 52 183 78 26 33
Fragments arranged by size:
541 531 380 278 275 261 251 222
206 183 134 103 92 78 52 45
43 33 26 21 20 16 14 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 555 642 1690 1953 2388 2804 2855 2957
Size: 555 87 1048 263 435 416 51 102
Cuts at: 2957 3813
Size: 856
Fragments arranged by size:
1048 856 555 435 416 263 102 87
51
BsrBI CCG'CTC
Cuts at: 0 1559 3813
Size: 1559 2254
BsrDI GCAATG_nn'
Cuts at: 0 456 2949 3813
Size: 456 2493 864
Fragments arranged by size:
2493 864 456
BsrGI T'GTAC_A
Cuts at: 0 3134 3813
Size: 3134 679
BstEII G'GTnAC_C
Cuts at: 0 2268 3813
Size: 2268 1545
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 3813
Size: 27 3786
BstYI r'GATC_y
Cuts at: 0 238 1025 3302 3813
Size: 238 787 2277 511
Fragments arranged by size:
2277 787 511 238
Bsu36I CC'TnA_GG
Cuts at: 0 2050 3813
Size: 2050 1763
BtgI C'CryG_G
Cuts at: 0 1014 1508 1631 1738 2078 2636 2730 2957
Size: 1014 494 123 107 340 558 94 227
Cuts at: 2957 2970 3359 3813
Size: 13 389 454
Fragments arranged by size:
1014 558 494 454 389 340 227 123
107 94 13
BtsI GCAGTG_nn'
Cuts at: 0 650 1198 1715 2622 3428 3458 3770 3813
Size: 650 548 517 907 806 30 312 43
Fragments arranged by size:
907 806 650 548 517 312 43 30
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 502 537 1741 1776 3813
Size: 502 35 1204 35 2037
Fragments arranged by size:
2037 1204 502 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1560 3481 3813
Size: 1560 1921 332
Fragments arranged by size:
1921 1560 332
EaeI y'GGCC_r
Cuts at: 0 523 1544 2132 2429 2633 2819 2954 3155
Size: 523 1021 588 297 204 186 135 201
Cuts at: 3155 3813
Size: 658
Fragments arranged by size:
1021 658 588 523 297 204 201 186
135
EagI C'GGCC_G
Cuts at: 0 3155 3813
Size: 3155 658
EarI CTCTTCn'nnn_
Cuts at: 0 233 1250 1790 2657 3597 3813
Size: 233 1017 540 867 940 216
Fragments arranged by size:
1017 940 867 540 233 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 237 3813
Size: 237 3576
EcoICRI GAG'CTC
Cuts at: 0 938 2898 3813
Size: 938 1960 915
Fragments arranged by size:
1960 938 915
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 255 306 964 1240 1309 1348 1393
Size: 98 157 51 658 276 69 39 45
Cuts at: 1393 1735 2110 2235 2284 2629 2824 3630 3813
Size: 342 375 125 49 345 195 806 183
Fragments arranged by size:
806 658 375 345 342 276 195 183
157 125 98 69 51 49 45 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 252 1807 2851 2875 3813
Size: 252 1555 1044 24 938
Fragments arranged by size:
1555 1044 938 252 24
EcoO109I rG'GnC_Cy
Cuts at: 0 45 54 90 119 278 359 385 620
Size: 45 9 36 29 159 81 26 235
Cuts at: 620 756 819 1497 1597 1598 1800 2034 2055
Size: 136 63 678 100 1 202 234 21
Cuts at: 2055 2259 2526 2844 3468 3813
Size: 204 267 318 624 345
Fragments arranged by size:
678 624 345 318 267 235 234 204
202 159 136 100 81 63 45 36
29 26 21 9 1
EcoRI G'AATT_C
Cuts at: 0 1092 3813
Size: 1092 2721
EcoRV GAT'ATC
Cuts at: 0 3702 3813
Size: 3702 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 430 1819 3162 3813
Size: 430 1389 1343 651
Fragments arranged by size:
1389 1343 651 430
HaeII r_GCGC'y
Cuts at: 0 1508 1981 2616 3813
Size: 1508 473 635 1197
Fragments arranged by size:
1508 1197 635 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 871 903 1378 1405 1410 1437
Size: 153 32 686 32 475 27 5 27
Cuts at: 1437 1638 1670 2026 2058 2331 2363 2773 2805
Size: 201 32 356 32 273 32 410 32
Cuts at: 2805 3698 3730 3813
Size: 893 32 83
Fragments arranged by size:
893 686 475 410 356 273 201 153
83 32 32 32 32 32 32 32
27 27 5
HincII GTy'rAC
Cuts at: 0 966 3813
Size: 966 2847
HindIII A'AGCT_T
Cuts at: 0 1180 3813
Size: 1180 2633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2690 3813
Size: 2690 1123
MscI TGG'CCA
Cuts at: 0 525 1546 2134 2821 2956 3813
Size: 525 1021 588 687 135 857
Fragments arranged by size:
1021 857 687 588 525 135
MslI CAynn'nnrTG
Cuts at: 0 1865 2243 2942 3520 3813
Size: 1865 378 699 578 293
Fragments arranged by size:
1865 699 578 378 293
MspA1I CmG'CkG
Cuts at: 0 473 799 971 978 1371 1443 1589 2304
Size: 473 326 172 7 393 72 146 715
Cuts at: 2304 2760 2864 2931 2972 3006 3520 3813
Size: 456 104 67 41 34 514 293
Fragments arranged by size:
715 514 473 456 393 326 293 172
146 104 72 67 41 34 7
NcoI C'CATG_G
Cuts at: 0 1014 2730 2957 3359 3813
Size: 1014 1716 227 402 454
Fragments arranged by size:
1716 1014 454 402 227
NspI r_CATG'y
Cuts at: 0 2317 3064 3813
Size: 2317 747 749
Fragments arranged by size:
2317 749 747
PasI CC'CwG_GG
Cuts at: 0 27 616 1728 2007 2014 2216 2523 2877
Size: 27 589 1112 279 7 202 307 354
Cuts at: 2877 3813
Size: 936
Fragments arranged by size:
1112 936 589 354 307 279 202 27
7
PflMI CCAn_nnn'nTGG
Cuts at: 0 1371 2130 3108 3351 3813
Size: 1371 759 978 243 462
Fragments arranged by size:
1371 978 759 462 243
PfoI T'CCnGG_A
Cuts at: 0 207 752 1187 1382 1400 1481 2600 2840
Size: 207 545 435 195 18 81 1119 240
Cuts at: 2840 3813
Size: 973
Fragments arranged by size:
1119 973 545 435 240 207 195 81
18
PpuMI rG'GwC_Cy
Cuts at: 0 54 90 119 359 385 620 756 2034
Size: 54 36 29 240 26 235 136 1278
Cuts at: 2034 2844 3468 3813
Size: 810 624 345
Fragments arranged by size:
1278 810 624 345 240 235 136 54
36 29 26
PshAI GACnn'nnGTC
Cuts at: 0 578 3289 3813
Size: 578 2711 524
Fragments arranged by size:
2711 578 524
PspOMI G'GGCC_C
Cuts at: 0 278 1597 2010 3813
Size: 278 1319 413 1803
Fragments arranged by size:
1803 1319 413 278
PstI C_TGCA'G
Cuts at: 0 67 219 291 596 1683 1769 3765 3813
Size: 67 152 72 305 1087 86 1996 48
Fragments arranged by size:
1996 1087 305 152 86 72 67 48
PvuII CAG'CTG
Cuts at: 0 799 971 978 1371 1443 1589 2864 2931
Size: 799 172 7 393 72 146 1275 67
Cuts at: 2931 3006 3813
Size: 75 807
Fragments arranged by size:
1275 807 799 393 172 146 75 72
67 7
SacI G_AGCT'C
Cuts at: 0 940 2900 3813
Size: 940 1960 913
Fragments arranged by size:
1960 940 913
SacII CC_GC'GG
Cuts at: 0 2973 3813
Size: 2973 840
SanDI GG'GwC_CC
Cuts at: 0 119 359 385 620 2034 3468 3813
Size: 119 240 26 235 1414 1434 345
Fragments arranged by size:
1434 1414 345 240 235 119 26
SapI GCTCTTCn'nnn_
Cuts at: 0 1250 2657 3813
Size: 1250 1407 1156
Fragments arranged by size:
1407 1250 1156
SfcI C'TryA_G
Cuts at: 0 63 215 287 592 1008 1087 1438 1679
Size: 63 152 72 305 416 79 351 241
Cuts at: 1679 1765 2279 2688 3761 3813
Size: 86 514 409 1073 52
Fragments arranged by size:
1073 514 416 409 351 305 241 152
86 79 72 63 52
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2080 3813
Size: 2080 1733
SmaI CCC'GGG
Cuts at: 0 145 1695 3662 3813
Size: 145 1550 1967 151
Fragments arranged by size:
1967 1550 151 145
SmlI C'TyrA_G
Cuts at: 0 2851 3195 3813
Size: 2851 344 618
Fragments arranged by size:
2851 618 344
SpeI A'CTAG_T
Cuts at: 0 2242 3813
Size: 2242 1571
SphI G_CATG'C
Cuts at: 0 2317 3813
Size: 2317 1496
StuI AGG'CCT
Cuts at: 0 327 900 1687 3813
Size: 327 573 787 2126
Fragments arranged by size:
2126 787 573 327
StyI C'CwwG_G
Cuts at: 0 339 1014 2630 2730 2957 3359 3813
Size: 339 675 1616 100 227 402 454
Fragments arranged by size:
1616 675 454 402 339 227 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 207 233 276 1129 1328 2137 3301 3769
Size: 207 26 43 853 199 809 1164 468
Cuts at: 3769 3813
Size: 44
Fragments arranged by size:
1164 853 809 468 207 199 44 43
26
TatI w'GTAC_w
Cuts at: 0 722 2456 3134 3813
Size: 722 1734 678 679
Fragments arranged by size:
1734 722 679 678
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 338 370 1892 1924 2030 2062 3813
Size: 338 32 1522 32 106 32 1751
Fragments arranged by size:
1751 1522 338 106 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3466 3813
Size: 3466 347
XbaI T'CTAG_A
Cuts at: 0 171 3813
Size: 171 3642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 90 567 2085 2127 2868 3813
Size: 90 477 1518 42 741 945
Fragments arranged by size:
1518 945 741 477 90 42
XmaI C'CCGG_G
Cuts at: 0 143 1693 3660 3813
Size: 143 1550 1967 153
Fragments arranged by size:
1967 1550 153 143
XmnI GAAnn'nnTTC
Cuts at: 0 410 1096 3813
Size: 410 686 2717
Fragments arranged by size:
2717 686 410
ZraI GAC'GTC
Cuts at: 0 576 3126 3813
Size: 576 2550 687
Fragments arranged by size:
2550 687 576
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI
BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI
SapI SfcI SfiI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AscI AseI AsiSI
BaeI BbeI BclI BfrBI BmtI BsaBI BsiWI BsmI
BspEI BspHI BsrFI BssHII BssSI BstAPI BstBI BstZ17I
BtgZI ClaI DraI DraIII FseI FspI FspAI HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI RsrII SalI SbfI
ScaI SexAI SfoI SgfI SgrAI SnaBI SrfI SspI
SwaI XhoI