Restriction map for
pF1KA1643
(Linear) (Six-base) MAPSORT of: KIAA1643.seq Check: 1980 from: 1 to: 2658
KIAA1643.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2571 2658
Size: 2571 87
AatII G_ACGT'C
Cuts at: 0 494 2658
Size: 494 2164
AccI GT'mk_AC
Cuts at: 0 1155 2658
Size: 1155 1503
Acc65I G'GTAC_C
Cuts at: 0 623 2658
Size: 623 2035
AfeI AGC'GCT
Cuts at: 0 476 2658
Size: 476 2182
AflIII A'CryG_T
Cuts at: 0 752 779 2615 2658
Size: 752 27 1836 43
Fragments arranged by size:
1836 752 43 27
AhdI GACnn_n'nnGTC
Cuts at: 0 1062 2658
Size: 1062 1596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 71 382 2658
Size: 71 311 2276
Fragments arranged by size:
2276 311 71
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1180 1212 2658
Size: 1180 32 1446
Fragments arranged by size:
1446 1180 32
AlwNI CAG_nnn'CTG
Cuts at: 0 435 816 1020 1283 1430 2034 2331 2658
Size: 435 381 204 263 147 604 297 327
Fragments arranged by size:
604 435 381 327 297 263 204 147
ApaI G_GGCC'C
Cuts at: 0 612 1815 2658
Size: 612 1203 843
Fragments arranged by size:
1203 843 612
ApaLI G'TGCA_C
Cuts at: 0 141 2357 2601 2658
Size: 141 2216 244 57
Fragments arranged by size:
2216 244 141 57
AvaI C'yCGr_G
Cuts at: 0 1243 1384 1755 2658
Size: 1243 141 371 903
Fragments arranged by size:
1243 903 371 141
BamHI G'GATC_C
Cuts at: 0 42 2658
Size: 42 2616
BanI G'GyrC_C
Cuts at: 0 307 415 534 623 915 1420 1771 1833
Size: 307 108 119 89 292 505 351 62
Cuts at: 1833 2022 2439 2658
Size: 189 417 219
Fragments arranged by size:
505 417 351 307 292 219 189 119
108 89 62
BanII G_rGCy'C
Cuts at: 0 515 612 1616 1815 1874 2008 2044 2658
Size: 515 97 1004 199 59 134 36 614
Fragments arranged by size:
1004 614 515 199 134 97 59 36
BbeI G_GCGC'C
Cuts at: 0 419 919 1837 2026 2658
Size: 419 500 918 189 632
Fragments arranged by size:
918 632 500 419 189
BbsI GAAGACnn'nnnn_
Cuts at: 0 755 2429 2658
Size: 755 1674 229
Fragments arranged by size:
1674 755 229
BbvCI CC'TCA_GC
Cuts at: 0 478 2658
Size: 478 2180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1923 1957 2658
Size: 1923 34 701
Fragments arranged by size:
1923 701 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1418 1760 2125 2658
Size: 1418 342 365 533
Fragments arranged by size:
1418 533 365 342
BglII A'GATC_T
Cuts at: 0 2528 2658
Size: 2528 130
BlpI GC'TnA_GC
Cuts at: 0 514 1030 1651 1791 2658
Size: 514 516 621 140 867
Fragments arranged by size:
867 621 516 514 140
Bme1580I G_kGCm'C
Cuts at: 0 145 612 1399 1423 1815 2361 2605 2658
Size: 145 467 787 24 392 546 244 53
Fragments arranged by size:
787 546 467 392 244 145 53 24
BmgBI CAC'GTC
Cuts at: 0 408 870 894 1212 1725 2658
Size: 408 462 24 318 513 933
Fragments arranged by size:
933 513 462 408 318 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1667 2658
Size: 1667 991
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1083 1623 2658
Size: 1083 540 1035
Fragments arranged by size:
1083 1035 540
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 421 1042 1133 2028 2658
Size: 373 48 621 91 895 630
Fragments arranged by size:
895 630 621 373 91 48
Bpu10I CC'TnA_GC
Cuts at: 0 478 962 2658
Size: 478 484 1696
Fragments arranged by size:
1696 484 478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1389 2658
Size: 1389 1269
BsaI GGTCTCn'nnnn_
Cuts at: 0 2365 2658
Size: 2365 293
BsaAI yAC'GTr
Cuts at: 0 564 2658
Size: 564 2094
BsaHI Gr'CG_yC
Cuts at: 0 416 491 916 1763 1834 2023 2658
Size: 416 75 425 847 71 189 635
Fragments arranged by size:
847 635 425 416 189 75 71
BsaWI w'CCGG_w
Cuts at: 0 1514 2144 2658
Size: 1514 630 514
Fragments arranged by size:
1514 630 514
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1180 1210 2362 2392 2658
Size: 1180 30 1152 30 266
Fragments arranged by size:
1180 1152 266 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 280 838 841 1198 1201 1375 1843
Size: 94 186 558 3 357 3 174 468
Cuts at: 1843 2061 2538 2658
Size: 218 477 120
Fragments arranged by size:
558 477 468 357 218 186 174 120
94 3 3
BseYI C'CCAG_C
Cuts at: 0 134 1101 1251 1466 1954 1999 2059 2100
Size: 134 967 150 215 488 45 60 41
Cuts at: 2100 2658
Size: 558
Fragments arranged by size:
967 558 488 215 150 134 60 45
41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 817 916 1438 1770 2448 2658
Size: 586 231 99 522 332 678 210
Fragments arranged by size:
678 586 522 332 231 210 99
BsiEI CG_ry'CG
Cuts at: 0 1589 2658
Size: 1589 1069
BsiHKAI G_wGCw'C
Cuts at: 0 145 515 587 2361 2605 2658
Size: 145 370 72 1774 244 53
Fragments arranged by size:
1774 370 244 145 72 53
BsiWI C'GTAC_G
Cuts at: 0 1778 2658
Size: 1778 880
BsmI GAATG_Cn'
Cuts at: 0 1680 2658
Size: 1680 978
BsmBI CGTCTCn'nnnn_
Cuts at: 0 528 589 1486 2658
Size: 528 61 897 1172
Fragments arranged by size:
1172 897 528 61
Bsp1286I G_dGCh'C
Cuts at: 0 145 515 587 612 1399 1423 1616 1815
Size: 145 370 72 25 787 24 193 199
Cuts at: 1815 1874 2008 2044 2361 2605 2658
Size: 59 134 36 317 244 53
Fragments arranged by size:
787 370 317 244 199 193 145 134
72 59 53 36 25 24
BspHI T'CATG_A
Cuts at: 0 338 2658
Size: 338 2320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1954 2571 2658
Size: 1954 617 87
Fragments arranged by size:
1954 617 87
BsrBI CCG'CTC
Cuts at: 0 167 1120 1327 2658
Size: 167 953 207 1331
Fragments arranged by size:
1331 953 207 167
BsrDI GCAATG_nn'
Cuts at: 0 1085 2658
Size: 1085 1573
BsrFI r'CCGG_y
Cuts at: 0 289 325 657 1786 2589 2650 2658
Size: 289 36 332 1129 803 61 8
Fragments arranged by size:
1129 803 332 289 61 36 8
BssSI C'ACGA_G
Cuts at: 0 703 2017 2658
Size: 703 1314 641
Fragments arranged by size:
1314 703 641
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2471 2609 2658
Size: 2471 138 49
Fragments arranged by size:
2471 138 49
BstEII G'GTnAC_C
Cuts at: 0 2319 2658
Size: 2319 339
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1268 1575 2658
Size: 1268 307 1083
Fragments arranged by size:
1268 1083 307
BstYI r'GATC_y
Cuts at: 0 42 269 2141 2528 2658
Size: 42 227 1872 387 130
Fragments arranged by size:
1872 387 227 130 42
Bsu36I CC'TnA_GG
Cuts at: 0 263 944 1911 2027 2658
Size: 263 681 967 116 631
Fragments arranged by size:
967 681 631 263 116
BtgI C'CryG_G
Cuts at: 0 98 530 1541 1616 2157 2658
Size: 98 432 1011 75 541 501
Fragments arranged by size:
1011 541 501 432 98 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2423 2658
Size: 2423 235
BtsI GCAGTG_nn'
Cuts at: 0 1182 2508 2607 2658
Size: 1182 1326 99 51
Fragments arranged by size:
1326 1182 99 51
DraIII CAC_nnn'GTG
Cuts at: 0 2471 2658
Size: 2471 187
DrdI GACnn_nn'nnGTC
Cuts at: 0 1585 2658
Size: 1585 1073
EaeI y'GGCC_r
Cuts at: 0 95 287 291 320 362 377 527 686
Size: 95 192 4 29 42 15 150 159
Cuts at: 686 1235 1619 2276 2658
Size: 549 384 657 382
Fragments arranged by size:
657 549 384 382 192 159 150 95
42 29 15 4
EarI CTCTTCn'nnn_
Cuts at: 0 592 2658
Size: 592 2066
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 138 1337 2485 2658
Size: 138 1199 1148 173
Fragments arranged by size:
1199 1148 173 138
EcoICRI GAG'CTC
Cuts at: 0 513 2658
Size: 513 2145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 421 1042 1133 2028 2658
Size: 373 48 621 91 895 630
Fragments arranged by size:
895 630 621 373 91 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 631 2658
Size: 631 2027
EcoO109I rG'GnC_Cy
Cuts at: 0 129 441 608 609 901 1811 1812 2031
Size: 129 312 167 1 292 910 1 219
Cuts at: 2031 2063 2658
Size: 32 595
Fragments arranged by size:
910 595 312 292 219 167 129 32
1 1
FseI GG_CCGG'CC
Cuts at: 0 293 2654 2658
Size: 293 2361 4
Fragments arranged by size:
2361 293 4
HaeII r_GCGC'y
Cuts at: 0 419 436 478 919 1837 2026 2658
Size: 419 17 42 441 918 189 632
Fragments arranged by size:
918 632 441 419 189 42 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 59 1048 1080 2126 2158 2362 2394
Size: 27 32 989 32 1046 32 204 32
Cuts at: 2394 2658
Size: 264
Fragments arranged by size:
1046 989 264 204 32 32 32 32
27
HindIII A'AGCT_T
Cuts at: 0 1648 2658
Size: 1648 1010
KasI G'GCGC_C
Cuts at: 0 415 915 1833 2022 2658
Size: 415 500 918 189 636
Fragments arranged by size:
918 636 500 415 189
KpnI G_GTAC'C
Cuts at: 0 627 2658
Size: 627 2031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 1883 1913 2658
Size: 20 1863 30 745
Fragments arranged by size:
1863 745 30 20
MscI TGG'CCA
Cuts at: 0 364 379 688 1237 2278 2658
Size: 364 15 309 549 1041 380
Fragments arranged by size:
1041 549 380 364 309 15
MslI CAynn'nnrTG
Cuts at: 0 337 364 1266 2658
Size: 337 27 902 1392
Fragments arranged by size:
1392 902 337 27
MspA1I CmG'CkG
Cuts at: 0 51 100 138 297 375 907 1255 1436
Size: 51 49 38 159 78 532 348 181
Cuts at: 1436 2059 2217 2519 2565 2658
Size: 623 158 302 46 93
Fragments arranged by size:
623 532 348 302 181 159 158 93
78 51 49 46 38
NaeI GCC'GGC
Cuts at: 0 291 327 659 1788 2652 2658
Size: 291 36 332 1129 864 6
Fragments arranged by size:
1129 864 332 291 36 6
NarI GG'CG_CC
Cuts at: 0 416 916 1834 2023 2658
Size: 416 500 918 189 635
Fragments arranged by size:
918 635 500 416 189
NcoI C'CATG_G
Cuts at: 0 530 2658
Size: 530 2128
NgoMIV G'CCGG_C
Cuts at: 0 289 325 657 1786 2650 2658
Size: 289 36 332 1129 864 8
Fragments arranged by size:
1129 864 332 289 36 8
NspI r_CATG'y
Cuts at: 0 756 1999 2116 2619 2658
Size: 756 1243 117 503 39
Fragments arranged by size:
1243 756 503 117 39
PasI CC'CwG_GG
Cuts at: 0 551 2658
Size: 551 2107
PciI A'CATG_T
Cuts at: 0 752 2615 2658
Size: 752 1863 43
Fragments arranged by size:
1863 752 43
PflMI CCAn_nnn'nTGG
Cuts at: 0 207 870 1020 2658
Size: 207 663 150 1638
Fragments arranged by size:
1638 663 207 150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 836 868 1180 1212 2658
Size: 836 32 312 32 1446
Fragments arranged by size:
1446 836 312 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 129 441 901 2063 2658
Size: 129 312 460 1162 595
Fragments arranged by size:
1162 595 460 312 129
PspOMI G'GGCC_C
Cuts at: 0 608 1811 2658
Size: 608 1203 847
Fragments arranged by size:
1203 847 608
PstI C_TGCA'G
Cuts at: 0 812 1435 2222 2658
Size: 812 623 787 436
Fragments arranged by size:
812 787 623 436
PvuII CAG'CTG
Cuts at: 0 51 297 1255 1436 2059 2217 2519 2658
Size: 51 246 958 181 623 158 302 139
Fragments arranged by size:
958 623 302 246 181 158 139 51
RsrII CG'GwC_CG
Cuts at: 0 2592 2658
Size: 2592 66
SacI G_AGCT'C
Cuts at: 0 515 2658
Size: 515 2143
SacII CC_GC'GG
Cuts at: 0 101 2658
Size: 101 2557
SanDI GG'GwC_CC
Cuts at: 0 901 2658
Size: 901 1757
SapI GCTCTTCn'nnn_
Cuts at: 0 592 2658
Size: 592 2066
ScaI AGT'ACT
Cuts at: 0 559 2658
Size: 559 2099
SexAI A'CCwGG_T
Cuts at: 0 89 2658
Size: 89 2569
SfcI C'TryA_G
Cuts at: 0 808 1431 2218 2637 2658
Size: 808 623 787 419 21
Fragments arranged by size:
808 787 623 419 21
SfoI GGC'GCC
Cuts at: 0 417 917 1835 2024 2658
Size: 417 500 918 189 634
Fragments arranged by size:
918 634 500 417 189
SmaI CCC'GGG
Cuts at: 0 1757 2658
Size: 1757 901
SmlI C'TyrA_G
Cuts at: 0 1368 1384 2658
Size: 1368 16 1274
Fragments arranged by size:
1368 1274 16
SphI G_CATG'C
Cuts at: 0 1999 2116 2658
Size: 1999 117 542
Fragments arranged by size:
1999 542 117
StuI AGG'CCT
Cuts at: 0 1144 2452 2658
Size: 1144 1308 206
Fragments arranged by size:
1308 1144 206
StyI C'CwwG_G
Cuts at: 0 380 530 1796 2345 2658
Size: 380 150 1266 549 313
Fragments arranged by size:
1266 549 380 313 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1256 1465 1609 2338 2427 2620 2658
Size: 1256 209 144 729 89 193 38
Fragments arranged by size:
1256 729 209 193 144 89 38
TatI w'GTAC_w
Cuts at: 0 557 2658
Size: 557 2101
Tth111I GACn'n_nGTC
Cuts at: 0 2376 2658
Size: 2376 282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 372 2658
Size: 372 2286
XhoI C'TCGA_G
Cuts at: 0 1384 2658
Size: 1384 1274
XmaI C'CCGG_G
Cuts at: 0 1755 2658
Size: 1755 903
XmnI GAAnn'nnTTC
Cuts at: 0 7 2530 2658
Size: 7 2523 128
Fragments arranged by size:
2523 128 7
ZraI GAC'GTC
Cuts at: 0 492 2658
Size: 492 2166
Enzymes that do cut:
AarI AatII AccI Acc65I AfeI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PpiI PpuMI PspOMI
PstI PvuII RsrII SacI SacII SanDI SapI ScaI
SexAI SfcI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AcuI AflII AgeI AleI ApoI AscI AseI
AsiSI AvrII BaeI BciVI BclI BfrBI BmtI BsaBI
BspEI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI
EagI EcoRI EcoRV FalI FspI FspAI HincII HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
SalI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI TstI XbaI