Restriction map for
pF1KA1531
(Linear) (Six-base) MAPSORT of: KIAA1531.seq Check: 6584 from: 1 to: 3363
KIAA1531.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 599 1508 3363
Size: 599 909 1855
Fragments arranged by size:
1855 909 599
AatII G_ACGT'C
Cuts at: 0 3290 3363
Size: 3290 73
AccI GT'mk_AC
Cuts at: 0 1430 1667 3363
Size: 1430 237 1696
Fragments arranged by size:
1696 1430 237
Acc65I G'GTAC_C
Cuts at: 0 842 3363
Size: 842 2521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1077 3363
Size: 421 656 2286
Fragments arranged by size:
2286 656 421
AfeI AGC'GCT
Cuts at: 0 2476 3363
Size: 2476 887
AflII C'TTAA_G
Cuts at: 0 544 3363
Size: 544 2819
AleI CACnn'nnGTG
Cuts at: 0 1159 1273 1288 2555 3363
Size: 1159 114 15 1267 808
Fragments arranged by size:
1267 1159 808 114 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1498 1699 1888 3363
Size: 1498 201 189 1475
Fragments arranged by size:
1498 1475 201 189
AlwNI CAG_nnn'CTG
Cuts at: 0 684 3363
Size: 684 2679
ApaI G_GGCC'C
Cuts at: 0 615 726 2363 2643 2690 2746 3363
Size: 615 111 1637 280 47 56 617
Fragments arranged by size:
1637 617 615 280 111 56 47
ApaLI G'TGCA_C
Cuts at: 0 981 2875 3363
Size: 981 1894 488
Fragments arranged by size:
1894 981 488
ApoI r'AATT_y
Cuts at: 0 1414 3363
Size: 1414 1949
AscI GG'CGCG_CC
Cuts at: 0 98 2589 3363
Size: 98 2491 774
Fragments arranged by size:
2491 774 98
AvaI C'yCGr_G
Cuts at: 0 85 92 138 229 1182 1190 2356 2486
Size: 85 7 46 91 953 8 1166 130
Cuts at: 2486 2636 2840 3060 3363
Size: 150 204 220 303
Fragments arranged by size:
1166 953 303 220 204 150 130 91
85 46 8 7
BanI G'GyrC_C
Cuts at: 0 4 30 81 253 376 562 842 1177
Size: 4 26 51 172 123 186 280 335
Cuts at: 1177 1186 1549 1639 1950 2125 2836 3064 3259
Size: 9 363 90 311 175 711 228 195
Cuts at: 3259 3363
Size: 104
Fragments arranged by size:
711 363 335 311 280 228 195 186
175 172 123 104 90 51 26 9
4
BanII G_rGCy'C
Cuts at: 0 236 296 615 726 858 1940 2363 2460
Size: 236 60 319 111 132 1082 423 97
Cuts at: 2460 2643 2690 2746 3092 3363
Size: 183 47 56 346 271
Fragments arranged by size:
1082 423 346 319 271 236 183 132
111 97 60 56 47
BbeI G_GCGC'C
Cuts at: 0 8 34 85 380 1643 2129 3068 3263
Size: 8 26 51 295 1263 486 939 195
Cuts at: 3263 3363
Size: 100
Fragments arranged by size:
1263 939 486 295 195 100 51 26
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 2535 3337 3363
Size: 2535 802 26
Fragments arranged by size:
2535 802 26
BbvCI CC'TCA_GC
Cuts at: 0 1579 2266 3363
Size: 1579 687 1097
Fragments arranged by size:
1579 1097 687
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1054 1088 1524 1558 3363
Size: 1054 34 436 34 1805
Fragments arranged by size:
1805 1054 436 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1157 3363
Size: 1157 2206
BglI GCCn_nnn'nGGC
Cuts at: 0 156 2446 2455 3155 3257 3363
Size: 156 2290 9 700 102 106
Fragments arranged by size:
2290 700 156 106 102 9
BglII A'GATC_T
Cuts at: 0 411 3363
Size: 411 2952
BlpI GC'TnA_GC
Cuts at: 0 160 1612 3363
Size: 160 1452 1751
Fragments arranged by size:
1751 1452 160
Bme1580I G_kGCm'C
Cuts at: 0 565 615 669 726 985 1166 1182 1189
Size: 565 50 54 57 259 181 16 7
Cuts at: 1189 1554 1953 2363 2643 2690 2746 2839 2879
Size: 365 399 410 280 47 56 93 40
Cuts at: 2879 3363
Size: 484
Fragments arranged by size:
565 484 410 399 365 280 259 181
93 57 56 54 50 47 40 16
7
BmgBI CAC'GTC
Cuts at: 0 2574 3363
Size: 2574 789
BmrI ACTGGGnnnn_n'
Cuts at: 0 656 1985 2342 3363
Size: 656 1329 357 1021
Fragments arranged by size:
1329 1021 656 357
BmtI G_CTAG'C
Cuts at: 0 1367 3363
Size: 1367 1996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 489 679 748 1057 1576 1965 2042
Size: 349 140 190 69 309 519 389 77
Cuts at: 2042 2269 3004 3226 3363
Size: 227 735 222 137
Fragments arranged by size:
735 519 389 349 309 227 222 190
140 137 77 69
Bpu10I CC'TnA_GC
Cuts at: 0 677 1579 2266 2284 3363
Size: 677 902 687 18 1079
Fragments arranged by size:
1079 902 687 677 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 2683 2935 3172 3363
Size: 291 2392 252 237 191
Fragments arranged by size:
2392 291 252 237 191
BsaI GGTCTCn'nnnn_
Cuts at: 0 442 1033 3363
Size: 442 591 2330
Fragments arranged by size:
2330 591 442
BsaBI GATnn'nnATC
Cuts at: 0 1300 3363
Size: 1300 2063
BsaHI Gr'CG_yC
Cuts at: 0 5 31 82 170 377 1640 2112 2126
Size: 5 26 51 88 207 1263 472 14
Cuts at: 2126 2353 2886 3065 3260 3287 3363
Size: 227 533 179 195 27 76
Fragments arranged by size:
1263 533 472 227 207 195 179 88
76 51 27 26 14 5
BsaWI w'CCGG_w
Cuts at: 0 425 2003 3334 3363
Size: 425 1578 1331 29
Fragments arranged by size:
1578 1331 425 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 487 517 1720 1750 1867 1897 2977 3007
Size: 487 30 1203 30 117 30 1080 30
Cuts at: 3007 3363
Size: 356
Fragments arranged by size:
1203 1080 487 356 117 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1872 2275 3363
Size: 1872 403 1088
Fragments arranged by size:
1872 1088 403
BseYI C'CCAG_C
Cuts at: 0 704 1223 1514 1592 2471 3363
Size: 704 519 291 78 879 892
Fragments arranged by size:
892 879 704 519 291 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 388 718 900 1639 1659 1858 2517
Size: 222 166 330 182 739 20 199 659
Cuts at: 2517 2998 3363
Size: 481 365
Fragments arranged by size:
739 659 481 365 330 222 199 182
166 20
BsiEI CG_ry'CG
Cuts at: 0 1221 2807 3363
Size: 1221 1586 556
Fragments arranged by size:
1586 1221 556
BsiHKAI G_wGCw'C
Cuts at: 0 139 805 985 1499 1928 1940 2879 3363
Size: 139 666 180 514 429 12 939 484
Fragments arranged by size:
939 666 514 484 429 180 139 12
Bsp1286I G_dGCh'C
Cuts at: 0 139 236 296 565 615 669 726 805
Size: 139 97 60 269 50 54 57 79
Cuts at: 805 858 985 1166 1182 1189 1499 1554 1928
Size: 53 127 181 16 7 310 55 374
Cuts at: 1928 1940 1953 2363 2460 2643 2690 2746 2839
Size: 12 13 410 97 183 47 56 93
Cuts at: 2839 2879 3092 3363
Size: 40 213 271
Fragments arranged by size:
410 374 310 271 269 213 183 181
139 127 97 97 93 79 60 57
56 55 54 53 50 47 40 16
13 12 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 599 1508 2771 3363
Size: 599 909 1263 592
Fragments arranged by size:
1263 909 599 592
BsrBI CCG'CTC
Cuts at: 0 147 164 1978 3005 3227 3237 3363
Size: 147 17 1814 1027 222 10 126
Fragments arranged by size:
1814 1027 222 147 126 17 10
BsrDI GCAATG_nn'
Cuts at: 0 306 3363
Size: 306 3057
BsrFI r'CCGG_y
Cuts at: 0 1202 1216 2806 2829 2945 3067 3363
Size: 1202 14 1590 23 116 122 296
Fragments arranged by size:
1590 1202 296 122 116 23 14
BsrGI T'GTAC_A
Cuts at: 0 1280 1346 3363
Size: 1280 66 2017
Fragments arranged by size:
2017 1280 66
BssHII G'CGCG_C
Cuts at: 0 37 98 2339 2589 3200 3363
Size: 37 61 2241 250 611 163
Fragments arranged by size:
2241 611 250 163 61 37
BssSI C'ACGA_G
Cuts at: 0 2583 3363
Size: 2583 780
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1770 3363
Size: 1770 1593
BstEII G'GTnAC_C
Cuts at: 0 2415 3363
Size: 2415 948
BstXI CCAn_nnnn'nTGG
Cuts at: 0 816 1632 3363
Size: 816 816 1731
Fragments arranged by size:
1731 816 816
BstYI r'GATC_y
Cuts at: 0 411 2168 3363
Size: 411 1757 1195
Fragments arranged by size:
1757 1195 411
BstZ17I GTA'TAC
Cuts at: 0 1668 3363
Size: 1668 1695
Bsu36I CC'TnA_GG
Cuts at: 0 343 966 3363
Size: 343 623 2397
Fragments arranged by size:
2397 623 343
BtgI C'CryG_G
Cuts at: 0 61 992 1083 1661 2615 2880 3363
Size: 61 931 91 578 954 265 483
Fragments arranged by size:
954 931 578 483 265 91 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3205 3363
Size: 3205 158
BtsI GCAGTG_nn'
Cuts at: 0 322 2471 3363
Size: 322 2149 892
Fragments arranged by size:
2149 892 322
DraIII CAC_nnn'GTG
Cuts at: 0 1662 3363
Size: 1662 1701
EaeI y'GGCC_r
Cuts at: 0 893 1218 1469 2438 2724 2804 3363
Size: 893 325 251 969 286 80 559
Fragments arranged by size:
969 893 559 325 286 251 80
EagI C'GGCC_G
Cuts at: 0 1218 2804 3363
Size: 1218 1586 559
Fragments arranged by size:
1586 1218 559
EarI CTCTTCn'nnn_
Cuts at: 0 1705 2542 3340 3363
Size: 1705 837 798 23
Fragments arranged by size:
1705 837 798 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 204 2514 2580 3363
Size: 204 2310 66 783
Fragments arranged by size:
2310 783 204 66
EcoICRI GAG'CTC
Cuts at: 0 1938 3363
Size: 1938 1425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 421 489 679 748 1057 1077 1576
Size: 349 72 68 190 69 309 20 499
Cuts at: 1576 1965 2042 2269 3004 3226 3363
Size: 389 77 227 735 222 137
Fragments arranged by size:
735 499 389 349 309 227 222 190
137 77 72 69 68 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 697 1623 3363
Size: 697 926 1740
Fragments arranged by size:
1740 926 697
EcoO109I rG'GnC_Cy
Cuts at: 0 213 459 681 722 723 1971 2203 2240
Size: 213 246 222 41 1 1248 232 37
Cuts at: 2240 2360 2640 2686 2687 2743 2955 3363
Size: 120 280 46 1 56 212 408
Fragments arranged by size:
1248 408 280 246 232 222 213 212
120 56 46 41 37 1 1
HaeII r_GCGC'y
Cuts at: 0 8 34 85 380 1619 1643 2129 2413
Size: 8 26 51 295 1239 24 486 284
Cuts at: 2413 2478 2983 3068 3128 3165 3173 3263 3363
Size: 65 505 85 60 37 8 90 100
Fragments arranged by size:
1239 505 486 295 284 100 90 85
65 60 51 37 26 24 8 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 420 452 930 962 1314 1346 2977 2994
Size: 420 32 478 32 352 32 1631 17
Cuts at: 2994 3009 3026 3363
Size: 15 17 337
Fragments arranged by size:
1631 478 420 352 337 32 32 32
17 17 15
HincII GTy'rAC
Cuts at: 0 1431 2064 3363
Size: 1431 633 1299
Fragments arranged by size:
1431 1299 633
KasI G'GCGC_C
Cuts at: 0 4 30 81 376 1639 2125 3064 3259
Size: 4 26 51 295 1263 486 939 195
Cuts at: 3259 3363
Size: 104
Fragments arranged by size:
1263 939 486 295 195 104 51 26
4
KpnI G_GTAC'C
Cuts at: 0 846 3363
Size: 846 2517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 501 617 3363
Size: 443 58 116 2746
Fragments arranged by size:
2746 443 116 58
MscI TGG'CCA
Cuts at: 0 1471 2440 3363
Size: 1471 969 923
Fragments arranged by size:
1471 969 923
MslI CAynn'nnrTG
Cuts at: 0 955 1159 1273 1288 1603 1744 1795 2140
Size: 955 204 114 15 315 141 51 345
Cuts at: 2140 2555 3295 3363
Size: 415 740 68
Fragments arranged by size:
955 740 415 345 315 204 141 114
68 51 15
MspA1I CmG'CkG
Cuts at: 0 54 128 206 633 839 1085 1223 1338
Size: 54 74 78 427 206 246 138 115
Cuts at: 1338 2056 2294 2501 2617 2723 2733 2769 3078
Size: 718 238 207 116 106 10 36 309
Cuts at: 3078 3317 3363
Size: 239 46
Fragments arranged by size:
718 427 309 246 239 238 207 206
138 116 115 106 78 74 54 46
36 10
NaeI GCC'GGC
Cuts at: 0 1218 2808 2831 3069 3363
Size: 1218 1590 23 238 294
Fragments arranged by size:
1590 1218 294 238 23
NarI GG'CG_CC
Cuts at: 0 5 31 82 377 1640 2126 3065 3260
Size: 5 26 51 295 1263 486 939 195
Cuts at: 3260 3363
Size: 103
Fragments arranged by size:
1263 939 486 295 195 103 51 26
5
NcoI C'CATG_G
Cuts at: 0 992 3363
Size: 992 2371
NgoMIV G'CCGG_C
Cuts at: 0 1216 2806 2829 3067 3363
Size: 1216 1590 23 238 296
Fragments arranged by size:
1590 1216 296 238 23
NheI G'CTAG_C
Cuts at: 0 1363 3363
Size: 1363 2000
NspI r_CATG'y
Cuts at: 0 676 1770 3363
Size: 676 1094 1593
Fragments arranged by size:
1593 1094 676
PasI CC'CwG_GG
Cuts at: 0 608 684 1167 1625 2529 3363
Size: 608 76 483 458 904 834
Fragments arranged by size:
904 834 608 483 458 76
PflMI CCAn_nnn'nTGG
Cuts at: 0 1860 2122 2733 3363
Size: 1860 262 611 630
Fragments arranged by size:
1860 630 611 262
PfoI T'CCnGG_A
Cuts at: 0 218 695 1750 2391 3363
Size: 218 477 1055 641 972
Fragments arranged by size:
1055 972 641 477 218
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 632 664 3363
Size: 632 32 2699
Fragments arranged by size:
2699 632 32
PpuMI rG'GwC_Cy
Cuts at: 0 213 1971 2203 3363
Size: 213 1758 232 1160
Fragments arranged by size:
1758 1160 232 213
PspOMI G'GGCC_C
Cuts at: 0 611 722 2359 2639 2686 2742 3363
Size: 611 111 1637 280 47 56 621
Fragments arranged by size:
1637 621 611 280 111 56 47
PstI C_TGCA'G
Cuts at: 0 632 1145 1537 2061 2768 3077 3363
Size: 632 513 392 524 707 309 286
Fragments arranged by size:
707 632 524 513 392 309 286
PvuII CAG'CTG
Cuts at: 0 128 633 1338 2056 2501 2769 3078 3363
Size: 128 505 705 718 445 268 309 285
Fragments arranged by size:
718 705 505 445 309 285 268 128
SacI G_AGCT'C
Cuts at: 0 1940 3363
Size: 1940 1423
SacII CC_GC'GG
Cuts at: 0 1086 2618 3363
Size: 1086 1532 745
Fragments arranged by size:
1532 1086 745
SalI G'TCGA_C
Cuts at: 0 1429 3363
Size: 1429 1934
SanDI GG'GwC_CC
Cuts at: 0 1971 3363
Size: 1971 1392
SapI GCTCTTCn'nnn_
Cuts at: 0 3340 3363
Size: 3340 23
SfcI C'TryA_G
Cuts at: 0 628 1141 1533 2057 2764 3073 3363
Size: 628 513 392 524 707 309 290
Fragments arranged by size:
707 628 524 513 392 309 290
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2446 3155 3363
Size: 2446 709 208
Fragments arranged by size:
2446 709 208
SfoI GGC'GCC
Cuts at: 0 6 32 83 378 1641 2127 3066 3261
Size: 6 26 51 295 1263 486 939 195
Cuts at: 3261 3363
Size: 102
Fragments arranged by size:
1263 939 486 295 195 102 51 26
6
SgrAI Cr'CCGG_yG
Cuts at: 0 1202 3363
Size: 1202 2161
SmaI CCC'GGG
Cuts at: 0 1184 2358 2488 2638 2842 3062 3363
Size: 1184 1174 130 150 204 220 301
Fragments arranged by size:
1184 1174 301 220 204 150 130
SmlI C'TyrA_G
Cuts at: 0 270 544 2698 2950 3187 3363
Size: 270 274 2154 252 237 176
Fragments arranged by size:
2154 274 270 252 237 176
SphI G_CATG'C
Cuts at: 0 676 3363
Size: 676 2687
SrfI GCCC'GGGC
Cuts at: 0 2358 3062 3363
Size: 2358 704 301
Fragments arranged by size:
2358 704 301
StuI AGG'CCT
Cuts at: 0 1531 2932 3363
Size: 1531 1401 431
Fragments arranged by size:
1531 1401 431
StyI C'CwwG_G
Cuts at: 0 152 992 999 2121 2222 2909 3266 3363
Size: 152 840 7 1122 101 687 357 97
Fragments arranged by size:
1122 840 687 357 152 101 97 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1537 3363
Size: 1537 1826
TatI w'GTAC_w
Cuts at: 0 1280 1346 2432 3363
Size: 1280 66 1086 931
Fragments arranged by size:
1280 1086 931 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1867 1899 3363
Size: 1081 32 754 32 1464
Fragments arranged by size:
1464 1081 754 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 942 3286 3363
Size: 942 2344 77
Fragments arranged by size:
2344 942 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1017 1842 2766 3363
Size: 1017 825 924 597
Fragments arranged by size:
1017 924 825 597
XmaI C'CCGG_G
Cuts at: 0 1182 2356 2486 2636 2840 3060 3363
Size: 1182 1174 130 150 204 220 303
Fragments arranged by size:
1182 1174 303 220 204 150 130
ZraI GAC'GTC
Cuts at: 0 3288 3363
Size: 3288 75
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AleI
AlfI AlwNI ApaI ApaLI ApoI AscI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII SacI SacII SalI SanDI SapI
SfcI SfiI SfoI SgrAI SmaI SmlI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
AclI AflIII AgeI AhdI AloI AseI AsiSI AvrII
BaeI BamHI BclI BfrBI BplI BsaAI BsiWI BsmI
BsmBI BspEI BspHI BstBI ClaI CspCI DraI DrdI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
MfeI MluI NdeI NotI NruI NsiI PacI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SbfI ScaI SexAI SgfI SnaBI SpeI SspI SwaI
XbaI XhoI XmnI