Restriction map for
pF1KA1408
(Linear) (Six-base) MAPSORT of: KIAA1408.seq Check: 6144 from: 1 to: 3816
KIAA1408.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AccI GT'mk_AC
Cuts at: 0 936 3816
Size: 936 2880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 465 706 1640 3816
Size: 179 286 241 934 2176
Fragments arranged by size:
2176 934 286 241 179
AflII C'TTAA_G
Cuts at: 0 3304 3816
Size: 3304 512
AflIII A'CryG_T
Cuts at: 0 2125 2782 3816
Size: 2125 657 1034
Fragments arranged by size:
2125 1034 657
AhdI GACnn_n'nnGTC
Cuts at: 0 564 3816
Size: 564 3252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2237 3719 3816
Size: 2237 1482 97
Fragments arranged by size:
2237 1482 97
AlwNI CAG_nnn'CTG
Cuts at: 0 613 1224 3816
Size: 613 611 2592
Fragments arranged by size:
2592 613 611
ApaI G_GGCC'C
Cuts at: 0 3461 3816
Size: 3461 355
ApaLI G'TGCA_C
Cuts at: 0 667 3403 3816
Size: 667 2736 413
Fragments arranged by size:
2736 667 413
ApoI r'AATT_y
Cuts at: 0 588 1201 1233 1395 2587 3016 3816
Size: 588 613 32 162 1192 429 800
Fragments arranged by size:
1192 800 613 588 429 162 32
AseI AT'TA_AT
Cuts at: 0 884 1580 3816
Size: 884 696 2236
Fragments arranged by size:
2236 884 696
BamHI G'GATC_C
Cuts at: 0 2525 3816
Size: 2525 1291
BanII G_rGCy'C
Cuts at: 0 1121 1172 3461 3816
Size: 1121 51 2289 355
Fragments arranged by size:
2289 1121 355 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 167 209 3816
Size: 167 42 3607
Fragments arranged by size:
3607 167 42
BbvCI CC'TCA_GC
Cuts at: 0 1930 3448 3816
Size: 1930 1518 368
Fragments arranged by size:
1930 1518 368
BclI T'GATC_A
Cuts at: 0 2924 3816
Size: 2924 892
BfrBI ATG'CAT
Cuts at: 0 2214 2251 3816
Size: 2214 37 1565
Fragments arranged by size:
2214 1565 37
BglII A'GATC_T
Cuts at: 0 1275 1623 1743 2146 3783 3816
Size: 1275 348 120 403 1637 33
Fragments arranged by size:
1637 1275 403 348 120 33
BlpI GC'TnA_GC
Cuts at: 0 8 3816
Size: 8 3808
Bme1580I G_kGCm'C
Cuts at: 0 671 1821 3407 3461 3816
Size: 671 1150 1586 54 355
Fragments arranged by size:
1586 1150 671 355 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 3475 3816
Size: 3475 341
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1031 3764 3816
Size: 1031 2733 52
Fragments arranged by size:
2733 1031 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1105 1367 2135 2140 2258 2296 3704 3816
Size: 1105 262 768 5 118 38 1408 112
Fragments arranged by size:
1408 1105 768 262 118 112 38 5
Bpu10I CC'TnA_GC
Cuts at: 0 1245 1930 2738 3448 3816
Size: 1245 685 808 710 368
Fragments arranged by size:
1245 808 710 685 368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1268 1579 1675 3816
Size: 480 788 311 96 2141
Fragments arranged by size:
2141 788 480 311 96
BsaBI GATnn'nnATC
Cuts at: 0 2443 3816
Size: 2443 1373
BsaWI w'CCGG_w
Cuts at: 0 453 2324 3816
Size: 453 1871 1492
Fragments arranged by size:
1871 1492 453
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3798 3816
Size: 3798 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 772 2297 3187 3226 3816
Size: 772 1525 890 39 590
Fragments arranged by size:
1525 890 772 590 39
BseYI C'CCAG_C
Cuts at: 0 1434 3714 3816
Size: 1434 2280 102
Fragments arranged by size:
2280 1434 102
BsiHKAI G_wGCw'C
Cuts at: 0 671 1121 1172 2507 3407 3774 3816
Size: 671 450 51 1335 900 367 42
Fragments arranged by size:
1335 900 671 450 367 51 42
BsmI GAATG_Cn'
Cuts at: 0 1148 2253 3816
Size: 1148 1105 1563
Fragments arranged by size:
1563 1148 1105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3804 3816
Size: 3804 12
Bsp1286I G_dGCh'C
Cuts at: 0 671 1121 1172 1821 2507 3407 3461 3774
Size: 671 450 51 649 686 900 54 313
Cuts at: 3774 3816
Size: 42
Fragments arranged by size:
900 686 671 649 450 313 54 51
42
BspEI T'CCGG_A
Cuts at: 0 453 2324 3816
Size: 453 1871 1492
Fragments arranged by size:
1871 1492 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2985 3816
Size: 2985 831
BsrDI GCAATG_nn'
Cuts at: 0 602 1769 1882 2216 3816
Size: 602 1167 113 334 1600
Fragments arranged by size:
1600 1167 602 334 113
BsrGI T'GTAC_A
Cuts at: 0 2336 3816
Size: 2336 1480
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2211 3778 3816
Size: 2211 1567 38
Fragments arranged by size:
2211 1567 38
BstEII G'GTnAC_C
Cuts at: 0 2355 3816
Size: 2355 1461
BstYI r'GATC_y
Cuts at: 0 1275 1623 1681 1743 1914 2146 2343 2525
Size: 1275 348 58 62 171 232 197 182
Cuts at: 2525 3551 3783 3816
Size: 1026 232 33
Fragments arranged by size:
1275 1026 348 232 232 197 182 171
62 58 33
Bsu36I CC'TnA_GG
Cuts at: 0 2164 3816
Size: 2164 1652
BtsI GCAGTG_nn'
Cuts at: 0 81 128 2050 2616 3816
Size: 81 47 1922 566 1200
Fragments arranged by size:
1922 1200 566 81 47
DraI TTT'AAA
Cuts at: 0 1067 1449 2063 3816
Size: 1067 382 614 1753
Fragments arranged by size:
1753 1067 614 382
DraIII CAC_nnn'GTG
Cuts at: 0 2535 3816
Size: 2535 1281
EaeI y'GGCC_r
Cuts at: 0 2787 3816
Size: 2787 1029
EarI CTCTTCn'nnn_
Cuts at: 0 166 263 293 311 1828 3083 3164 3203
Size: 166 97 30 18 1517 1255 81 39
Cuts at: 3203 3212 3263 3614 3816
Size: 9 51 351 202
Fragments arranged by size:
1517 1255 351 202 166 97 81 51
39 30 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 99 3816
Size: 99 3717
EcoICRI GAG'CTC
Cuts at: 0 1119 1170 3816
Size: 1119 51 2646
Fragments arranged by size:
2646 1119 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 465 706 1105 1367 1640 2135 2140
Size: 179 286 241 399 262 273 495 5
Cuts at: 2140 2258 2296 3704 3816
Size: 118 38 1408 112
Fragments arranged by size:
1408 495 399 286 273 262 241 179
118 112 38 5
EcoO109I rG'GnC_Cy
Cuts at: 0 153 3457 3458 3816
Size: 153 3304 1 358
Fragments arranged by size:
3304 358 153 1
EcoRI G'AATT_C
Cuts at: 0 3016 3816
Size: 3016 800
EcoRV GAT'ATC
Cuts at: 0 120 3654 3816
Size: 120 3534 162
Fragments arranged by size:
3534 162 120
HaeII r_GCGC'y
Cuts at: 0 3714 3816
Size: 3714 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 550 582 883 915 1017 1049
Size: 238 32 280 32 301 32 102 32
Cuts at: 1049 1673 1705 2489 2521 2942 2974 3037 3069
Size: 624 32 784 32 421 32 63 32
Cuts at: 3069 3475 3507 3816
Size: 406 32 309
Fragments arranged by size:
784 624 421 406 309 301 280 238
102 63 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 937 1737 3521 3816
Size: 937 800 1784 295
Fragments arranged by size:
1784 937 800 295
HindIII A'AGCT_T
Cuts at: 0 398 3816
Size: 398 3418
HpaI GTT'AAC
Cuts at: 0 3521 3816
Size: 3521 295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1508 1544 1826 3816
Size: 1508 36 282 1990
Fragments arranged by size:
1990 1508 282 36
MscI TGG'CCA
Cuts at: 0 2789 3816
Size: 2789 1027
MslI CAynn'nnrTG
Cuts at: 0 908 1780 2219 3816
Size: 908 872 439 1597
Fragments arranged by size:
1597 908 872 439
MspA1I CmG'CkG
Cuts at: 0 7 352 2278 2659 3447 3816
Size: 7 345 1926 381 788 369
Fragments arranged by size:
1926 788 381 369 345 7
NsiI A_TGCA'T
Cuts at: 0 2216 2253 3816
Size: 2216 37 1563
Fragments arranged by size:
2216 1563 37
NspI r_CATG'y
Cuts at: 0 1899 2111 2129 2786 3313 3816
Size: 1899 212 18 657 527 503
Fragments arranged by size:
1899 657 527 503 212 18
PciI A'CATG_T
Cuts at: 0 2125 2782 3816
Size: 2125 657 1034
Fragments arranged by size:
2125 1034 657
PflMI CCAn_nnn'nTGG
Cuts at: 0 2535 3816
Size: 2535 1281
PfoI T'CCnGG_A
Cuts at: 0 2312 3816
Size: 2312 1504
PspOMI G'GGCC_C
Cuts at: 0 3457 3816
Size: 3457 359
PstI C_TGCA'G
Cuts at: 0 76 993 3498 3816
Size: 76 917 2505 318
Fragments arranged by size:
2505 917 318 76
PvuII CAG'CTG
Cuts at: 0 7 352 2278 2659 3447 3816
Size: 7 345 1926 381 788 369
Fragments arranged by size:
1926 788 381 369 345 7
SacI G_AGCT'C
Cuts at: 0 1121 1172 3816
Size: 1121 51 2644
Fragments arranged by size:
2644 1121 51
SalI G'TCGA_C
Cuts at: 0 935 3816
Size: 935 2881
SapI GCTCTTCn'nnn_
Cuts at: 0 311 3816
Size: 311 3505
ScaI AGT'ACT
Cuts at: 0 1075 2794 3816
Size: 1075 1719 1022
Fragments arranged by size:
1719 1075 1022
SexAI A'CCwGG_T
Cuts at: 0 1335 3816
Size: 1335 2481
SfcI C'TryA_G
Cuts at: 0 72 614 917 989 1714 3373 3494 3816
Size: 72 542 303 72 725 1659 121 322
Fragments arranged by size:
1659 725 542 322 303 121 72 72
SmlI C'TyrA_G
Cuts at: 0 495 1283 1558 1690 3304 3816
Size: 495 788 275 132 1614 512
Fragments arranged by size:
1614 788 512 495 275 132
SpeI A'CTAG_T
Cuts at: 0 2499 3816
Size: 2499 1317
SphI G_CATG'C
Cuts at: 0 2111 3816
Size: 2111 1705
SspI AAT'ATT
Cuts at: 0 879 1977 3816
Size: 879 1098 1839
Fragments arranged by size:
1839 1098 879
StuI AGG'CCT
Cuts at: 0 3035 3816
Size: 3035 781
StyI C'CwwG_G
Cuts at: 0 970 3816
Size: 970 2846
TatI w'GTAC_w
Cuts at: 0 1073 2175 2336 2792 3816
Size: 1073 1102 161 456 1024
Fragments arranged by size:
1102 1073 1024 456 161
XbaI T'CTAG_A
Cuts at: 0 1572 3816
Size: 1572 2244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 577 2178 2518 2536 3816
Size: 577 1601 340 18 1280
Fragments arranged by size:
1601 1280 577 340 18
XmnI GAAnn'nnTTC
Cuts at: 0 380 2555 3816
Size: 380 2175 1261
Fragments arranged by size:
2175 1261 380
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AlfI AlwNI ApaI
ApaLI ApoI AseI BamHI BanII BbsI BbvCI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrDI BsrGI BstAPI BstEII
BstYI Bsu36I BtsI DraI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII
HindIII HpaI MmeI MscI MslI MspA1I NsiI NspI
PciI PflMI PfoI PspOMI PstI PvuII SacI SalI
SapI ScaI SexAI SfcI SmlI SpeI SphI SspI
StuI StyI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AloI
AscI AsiSI AvaI AvrII BaeI BanI BbeI BcgI
BciVI BglI BmgBI BmtI BsaI BsaAI BsaHI BsgI
BsiEI BsiWI BspHI BsrBI BsrFI BssHII BssSI BstBI
BstXI BstZ17I BtgI BtgZI ClaI CspCI DrdI EagI
EcoNI FalI FseI FspI FspAI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SanDI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI
TaqII TstI Tth111I XhoI XmaI ZraI