Restriction map for
pF1KSDA1399
(Linear) (Six-base) MAPSORT of: KIAA1399.seq Check: 5125 from: 1 to: 1230
KIAA1399.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 387 1230
Size: 387 843
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1230
Size: 166 1064
AleI CACnn'nnGTG
Cuts at: 0 1127 1225 1230
Size: 1127 98 5
Fragments arranged by size:
1127 98 5
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1097 1230
Size: 1097 133
ApaI G_GGCC'C
Cuts at: 0 277 1081 1230
Size: 277 804 149
Fragments arranged by size:
804 277 149
ApaLI G'TGCA_C
Cuts at: 0 558 1230
Size: 558 672
AvaI C'yCGr_G
Cuts at: 0 278 1230
Size: 278 952
BanI G'GyrC_C
Cuts at: 0 164 247 1057 1230
Size: 164 83 810 173
Fragments arranged by size:
810 173 164 83
BanII G_rGCy'C
Cuts at: 0 277 302 532 733 1081 1230
Size: 277 25 230 201 348 149
Fragments arranged by size:
348 277 230 201 149 25
BbeI G_GCGC'C
Cuts at: 0 168 251 1230
Size: 168 83 979
Fragments arranged by size:
979 168 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 433 1230
Size: 433 797
BglII A'GATC_T
Cuts at: 0 821 1230
Size: 821 409
BlpI GC'TnA_GC
Cuts at: 0 391 526 1230
Size: 391 135 704
Fragments arranged by size:
704 391 135
Bme1580I G_kGCm'C
Cuts at: 0 78 277 562 1081 1230
Size: 78 199 285 519 149
Fragments arranged by size:
519 285 199 149 78
BmgBI CAC'GTC
Cuts at: 0 639 852 1230
Size: 639 213 378
Fragments arranged by size:
639 378 213
BmrI ACTGGGnnnn_n'
Cuts at: 0 363 457 1230
Size: 363 94 773
Fragments arranged by size:
773 363 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 627 988 1230
Size: 288 339 361 242
Fragments arranged by size:
361 339 288 242
Bpu10I CC'TnA_GC
Cuts at: 0 1070 1230
Size: 1070 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 158 1182 1230
Size: 97 61 1024 48
Fragments arranged by size:
1024 97 61 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 112 871 1042 1158 1230
Size: 112 759 171 116 72
Fragments arranged by size:
759 171 116 112 72
BsaHI Gr'CG_yC
Cuts at: 0 165 248 1230
Size: 165 83 982
Fragments arranged by size:
982 165 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 161 393 999 1026 1060 1230
Size: 100 61 232 606 27 34 170
Fragments arranged by size:
606 232 170 100 61 34 27
BseYI C'CCAG_C
Cuts at: 0 78 203 363 1230
Size: 78 125 160 867
Fragments arranged by size:
867 160 125 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1230
Size: 1129 101
BsiHKAI G_wGCw'C
Cuts at: 0 562 1230
Size: 562 668
Bsp1286I G_dGCh'C
Cuts at: 0 78 277 302 532 562 733 1081 1230
Size: 78 199 25 230 30 171 348 149
Fragments arranged by size:
348 230 199 171 149 78 30 25
BspHI T'CATG_A
Cuts at: 0 1170 1211 1230
Size: 1170 41 19
Fragments arranged by size:
1170 41 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 387 1230
Size: 387 843
BsrBI CCG'CTC
Cuts at: 0 597 1230
Size: 597 633
BsrFI r'CCGG_y
Cuts at: 0 1093 1230
Size: 1093 137
BstYI r'GATC_y
Cuts at: 0 821 1230
Size: 821 409
Bsu36I CC'TnA_GG
Cuts at: 0 1104 1230
Size: 1104 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 627 729 1230
Size: 627 102 501
Fragments arranged by size:
627 501 102
BtsI GCAGTG_nn'
Cuts at: 0 402 676 937 1230
Size: 402 274 261 293
Fragments arranged by size:
402 293 274 261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 308 343 1230
Size: 308 35 887
Fragments arranged by size:
887 308 35
DraI TTT'AAA
Cuts at: 0 906 1230
Size: 906 324
EaeI y'GGCC_r
Cuts at: 0 221 1095 1230
Size: 221 874 135
Fragments arranged by size:
874 221 135
EarI CTCTTCn'nnn_
Cuts at: 0 139 742 820 1037 1230
Size: 139 603 78 217 193
Fragments arranged by size:
603 217 193 139 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 288 627 988 1230
Size: 166 122 339 361 242
Fragments arranged by size:
361 339 242 166 122
EcoO109I rG'GnC_Cy
Cuts at: 0 273 274 476 883 1077 1078 1108 1230
Size: 273 1 202 407 194 1 30 122
Fragments arranged by size:
407 273 202 194 122 30 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 171 1230
Size: 171 1059
HaeII r_GCGC'y
Cuts at: 0 168 181 251 804 1230
Size: 168 13 70 553 426
Fragments arranged by size:
553 426 168 70 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 420 427 452 459 1193 1225 1230
Size: 420 7 25 7 734 32 5
Fragments arranged by size:
734 420 32 25 7 7 5
HincII GTy'rAC
Cuts at: 0 773 924 1230
Size: 773 151 306
Fragments arranged by size:
773 306 151
KasI G'GCGC_C
Cuts at: 0 164 247 1230
Size: 164 83 983
Fragments arranged by size:
983 164 83
MslI CAynn'nnrTG
Cuts at: 0 850 1127 1225 1230
Size: 850 277 98 5
Fragments arranged by size:
850 277 98 5
MspA1I CmG'CkG
Cuts at: 0 122 539 1230
Size: 122 417 691
Fragments arranged by size:
691 417 122
NaeI GCC'GGC
Cuts at: 0 1095 1230
Size: 1095 135
NarI GG'CG_CC
Cuts at: 0 165 248 1230
Size: 165 83 982
Fragments arranged by size:
982 165 83
NgoMIV G'CCGG_C
Cuts at: 0 1093 1230
Size: 1093 137
NspI r_CATG'y
Cuts at: 0 132 1230
Size: 132 1098
PpuMI rG'GwC_Cy
Cuts at: 0 476 883 1230
Size: 476 407 347
Fragments arranged by size:
476 407 347
PspOMI G'GGCC_C
Cuts at: 0 273 1077 1230
Size: 273 804 153
Fragments arranged by size:
804 273 153
PstI C_TGCA'G
Cuts at: 0 671 1230
Size: 671 559
SapI GCTCTTCn'nnn_
Cuts at: 0 139 1230
Size: 139 1091
SfcI C'TryA_G
Cuts at: 0 667 1230
Size: 667 563
SfoI GGC'GCC
Cuts at: 0 166 249 1230
Size: 166 83 981
Fragments arranged by size:
981 166 83
SmlI C'TyrA_G
Cuts at: 0 112 173 1161 1230
Size: 112 61 988 69
Fragments arranged by size:
988 112 69 61
SphI G_CATG'C
Cuts at: 0 132 1230
Size: 132 1098
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 540 1033 1230
Size: 445 95 493 197
Fragments arranged by size:
493 445 197 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 645 677 841 873 875 907 1230
Size: 645 32 164 32 2 32 323
Fragments arranged by size:
645 323 164 32 32 32 2
XmnI GAAnn'nnTTC
Cuts at: 0 151 1230
Size: 151 1079
Enzymes that do cut:
AarI AcuI AleI AlfI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BstYI Bsu36I
BtgZI BtsI CspCI DraI EaeI EarI Eco57MI EcoO109I
FalI HaeII Hin4I HincII KasI MslI MspA1I NaeI
NarI NgoMIV NspI PpuMI PspOMI PstI SapI SfcI
SfoI SmlI SphI TaqII TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BmtI BplI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI ClaI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI StyI SwaI TatI
Tth111I XbaI XcmI XhoI XmaI ZraI