Restriction map for
pF1KSDA1387
(Linear) (Six-base) MAPSORT of: KIAA1387.seq Check: 3259 from: 1 to: 2547
KIAA1387.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 584 962 1430 2547
Size: 371 213 378 468 1117
Fragments arranged by size:
1117 468 378 371 213
AflIII A'CryG_T
Cuts at: 0 406 2547
Size: 406 2141
AleI CACnn'nnGTG
Cuts at: 0 91 2547
Size: 91 2456
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1286 1318 2547
Size: 1286 32 1229
Fragments arranged by size:
1286 1229 32
AlwNI CAG_nnn'CTG
Cuts at: 0 769 2067 2547
Size: 769 1298 480
Fragments arranged by size:
1298 769 480
ApoI r'AATT_y
Cuts at: 0 287 718 855 1088 1174 1381 1389 1737
Size: 287 431 137 233 86 207 8 348
Cuts at: 1737 1807 1896 1921 2317 2547
Size: 70 89 25 396 230
Fragments arranged by size:
431 396 348 287 233 230 207 137
89 86 70 25 8
AseI AT'TA_AT
Cuts at: 0 205 1247 2547
Size: 205 1042 1300
Fragments arranged by size:
1300 1042 205
BamHI G'GATC_C
Cuts at: 0 2404 2547
Size: 2404 143
BanI G'GyrC_C
Cuts at: 0 67 2187 2547
Size: 67 2120 360
Fragments arranged by size:
2120 360 67
BanII G_rGCy'C
Cuts at: 0 104 2547
Size: 104 2443
BbsI GAAGACnn'nnnn_
Cuts at: 0 50 1457 2032 2279 2547
Size: 50 1407 575 247 268
Fragments arranged by size:
1407 575 268 247 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1973 2007 2547
Size: 1973 34 540
Fragments arranged by size:
1973 540 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 192 2547
Size: 192 2355
BfrBI ATG'CAT
Cuts at: 0 1060 1413 2547
Size: 1060 353 1134
Fragments arranged by size:
1134 1060 353
BglII A'GATC_T
Cuts at: 0 275 2547
Size: 275 2272
BlpI GC'TnA_GC
Cuts at: 0 698 1217 2547
Size: 698 519 1330
Fragments arranged by size:
1330 698 519
Bme1580I G_kGCm'C
Cuts at: 0 77 1770 2547
Size: 77 1693 777
Fragments arranged by size:
1693 777 77
BmgBI CAC'GTC
Cuts at: 0 78 2547
Size: 78 2469
Bpu10I CC'TnA_GC
Cuts at: 0 1286 2204 2547
Size: 1286 918 343
Fragments arranged by size:
1286 918 343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1544 2547
Size: 88 1456 1003
Fragments arranged by size:
1456 1003 88
BsaAI yAC'GTr
Cuts at: 0 93 407 2547
Size: 93 314 2140
Fragments arranged by size:
2140 314 93
BsaBI GATnn'nnATC
Cuts at: 0 1280 1610 2547
Size: 1280 330 937
Fragments arranged by size:
1280 937 330
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 1286 1316 2547
Size: 67 30 1189 30 1231
Fragments arranged by size:
1231 1189 67 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 72 112 450 2547
Size: 72 40 338 2097
Fragments arranged by size:
2097 338 72 40
BsiEI CG_ry'CG
Cuts at: 0 63 2547
Size: 63 2484
BsiHKAI G_wGCw'C
Cuts at: 0 104 458 2547
Size: 104 354 2089
Fragments arranged by size:
2089 354 104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 84 2547
Size: 84 2463
Bsp1286I G_dGCh'C
Cuts at: 0 77 104 458 1770 2547
Size: 77 27 354 1312 777
Fragments arranged by size:
1312 777 354 77 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 407 2547
Size: 407 2140
BsrDI GCAATG_nn'
Cuts at: 0 58 2547
Size: 58 2489
BsrFI r'CCGG_y
Cuts at: 0 47 2547
Size: 47 2500
BstXI CCAn_nnnn'nTGG
Cuts at: 0 93 2547
Size: 93 2454
BstYI r'GATC_y
Cuts at: 0 275 2404 2547
Size: 275 2129 143
Fragments arranged by size:
2129 275 143
ClaI AT'CG_AT
Cuts at: 0 1993 2547
Size: 1993 554
DraI TTT'AAA
Cuts at: 0 1478 2547
Size: 1478 1069
EarI CTCTTCn'nnn_
Cuts at: 0 422 845 1551 1713 2123 2159 2415 2486
Size: 422 423 706 162 410 36 256 71
Cuts at: 2486 2547
Size: 61
Fragments arranged by size:
706 423 422 410 256 162 71 61
36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1800 2547
Size: 1800 747
EcoICRI GAG'CTC
Cuts at: 0 102 2547
Size: 102 2445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 584 962 1430 2547
Size: 371 213 378 468 1117
Fragments arranged by size:
1117 468 378 371 213
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 1289 2547
Size: 613 676 1258
Fragments arranged by size:
1258 676 613
EcoO109I rG'GnC_Cy
Cuts at: 0 333 2547
Size: 333 2214
EcoRI G'AATT_C
Cuts at: 0 718 1088 1737 1896 2547
Size: 718 370 649 159 651
Fragments arranged by size:
718 651 649 370 159
EcoRV GAT'ATC
Cuts at: 0 1941 2547
Size: 1941 606
HaeII r_GCGC'y
Cuts at: 0 1301 2547
Size: 1301 1246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 11 43 134 166 1712 1744 1930 1962
Size: 11 32 91 32 1546 32 186 32
Cuts at: 1962 2547
Size: 585
Fragments arranged by size:
1546 585 186 91 32 32 32 32
11
HindIII A'AGCT_T
Cuts at: 0 539 2547
Size: 539 2008
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 1477 1554 2547
Size: 161 1316 77 993
Fragments arranged by size:
1316 993 161 77
MslI CAynn'nnrTG
Cuts at: 0 91 581 2547
Size: 91 490 1966
Fragments arranged by size:
1966 490 91
MspA1I CmG'CkG
Cuts at: 0 531 1144 1903 2547
Size: 531 613 759 644
Fragments arranged by size:
759 644 613 531
NsiI A_TGCA'T
Cuts at: 0 1062 1415 2547
Size: 1062 353 1132
Fragments arranged by size:
1132 1062 353
NspI r_CATG'y
Cuts at: 0 915 1350 1417 2547
Size: 915 435 67 1130
Fragments arranged by size:
1130 915 435 67
PmlI CAC'GTG
Cuts at: 0 407 2547
Size: 407 2140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1286 1318 2547
Size: 1286 32 1229
Fragments arranged by size:
1286 1229 32
PpuMI rG'GwC_Cy
Cuts at: 0 333 2547
Size: 333 2214
PsiI TTA'TAA
Cuts at: 0 1814 1855 1927 1979 2547
Size: 1814 41 72 52 568
Fragments arranged by size:
1814 568 72 52 41
PvuII CAG'CTG
Cuts at: 0 531 1144 1903 2547
Size: 531 613 759 644
Fragments arranged by size:
759 644 613 531
SacI G_AGCT'C
Cuts at: 0 104 2547
Size: 104 2443
SfcI C'TryA_G
Cuts at: 0 971 1148 2369 2547
Size: 971 177 1221 178
Fragments arranged by size:
1221 971 178 177
SmlI C'TyrA_G
Cuts at: 0 103 1559 2547
Size: 103 1456 988
Fragments arranged by size:
1456 988 103
SpeI A'CTAG_T
Cuts at: 0 382 2547
Size: 382 2165
StyI C'CwwG_G
Cuts at: 0 494 1829 2123 2450 2547
Size: 494 1335 294 327 97
Fragments arranged by size:
1335 494 327 294 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 77 2547
Size: 77 2470
TatI w'GTAC_w
Cuts at: 0 809 2547
Size: 809 1738
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 1584 1616 2547
Size: 67 32 1485 32 931
Fragments arranged by size:
1485 931 67 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2534 2547
Size: 2534 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2457 2547
Size: 2457 90
XmnI GAAnn'nnTTC
Cuts at: 0 2003 2285 2547
Size: 2003 282 262
Fragments arranged by size:
2003 282 262
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApoI AseI BamHI
BanI BanII BbsI BcgI BciVI BfrBI BglII BlpI
Bme1580I BmgBI Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BstXI
BstYI ClaI DraI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HindIII MmeI MslI
MspA1I NsiI NspI PmlI PpiI PpuMI PsiI PvuII
SacI SfcI SmlI SpeI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BbvCI BclI BglI BmrI BmtI BplI
BpmI BsaI BsaHI BsaWI BseYI BsgI BsiWI BsmI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EagI FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI StuI SwaI XbaI XhoI XmaI
ZraI