Restriction map for
pF1KSDA1152
(Linear) (Six-base) MAPSORT of: KIAA1152.seq Check: 8416 from: 1 to: 1053
KIAA1152.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 369 927 1053
Size: 369 558 126
Fragments arranged by size:
558 369 126
Acc65I G'GTAC_C
Cuts at: 0 905 1053
Size: 905 148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 460 845 1053
Size: 412 48 385 208
Fragments arranged by size:
412 385 208 48
AflIII A'CryG_T
Cuts at: 0 650 1053
Size: 650 403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 963 1053
Size: 963 90
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 126 158 1053
Size: 126 32 895
Fragments arranged by size:
895 126 32
AlwNI CAG_nnn'CTG
Cuts at: 0 438 501 1053
Size: 438 63 552
Fragments arranged by size:
552 438 63
ApoI r'AATT_y
Cuts at: 0 856 1053
Size: 856 197
BanI G'GyrC_C
Cuts at: 0 905 1053
Size: 905 148
BanII G_rGCy'C
Cuts at: 0 94 640 701 810 1053
Size: 94 546 61 109 243
Fragments arranged by size:
546 243 109 94 61
BbvCI CC'TCA_GC
Cuts at: 0 27 1053
Size: 27 1026
BclI T'GATC_A
Cuts at: 0 813 1053
Size: 813 240
BlpI GC'TnA_GC
Cuts at: 0 88 1053
Size: 88 965
BmrI ACTGGGnnnn_n'
Cuts at: 0 704 1053
Size: 704 349
BmtI G_CTAG'C
Cuts at: 0 425 1053
Size: 425 628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 1053
Size: 953 100
Bpu10I CC'TnA_GC
Cuts at: 0 27 580 1053
Size: 27 553 473
Fragments arranged by size:
553 473 27
BsaWI w'CCGG_w
Cuts at: 0 240 1053
Size: 240 813
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 752 782 1053
Size: 752 30 271
Fragments arranged by size:
752 271 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1053
Size: 91 962
BseYI C'CCAG_C
Cuts at: 0 291 1053
Size: 291 762
Bsp1286I G_dGCh'C
Cuts at: 0 94 640 701 810 1053
Size: 94 546 61 109 243
Fragments arranged by size:
546 243 109 94 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 181 464 1053
Size: 181 283 589
Fragments arranged by size:
589 283 181
BstEII G'GTnAC_C
Cuts at: 0 243 1053
Size: 243 810
BtgI C'CryG_G
Cuts at: 0 543 1053
Size: 543 510
BtsI GCAGTG_nn'
Cuts at: 0 678 693 1053
Size: 678 15 360
Fragments arranged by size:
678 360 15
EaeI y'GGCC_r
Cuts at: 0 889 1053
Size: 889 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 402 1053
Size: 402 651
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 460 845 953 1053
Size: 412 48 385 108 100
Fragments arranged by size:
412 385 108 100 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 955 1053
Size: 955 98
HaeII r_GCGC'y
Cuts at: 0 88 1053
Size: 88 965
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 28 42 60 637 669 752 767
Size: 10 18 14 18 577 32 83 15
Cuts at: 767 784 799 1053
Size: 17 15 254
Fragments arranged by size:
577 254 83 32 18 18 17 15
15 14 10
HincII GTy'rAC
Cuts at: 0 370 1053
Size: 370 683
HindIII A'AGCT_T
Cuts at: 0 58 1053
Size: 58 995
KpnI G_GTAC'C
Cuts at: 0 909 1053
Size: 909 144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1053
Size: 112 941
MspA1I CmG'CkG
Cuts at: 0 509 1053
Size: 509 544
NheI G'CTAG_C
Cuts at: 0 421 1053
Size: 421 632
NspI r_CATG'y
Cuts at: 0 654 1053
Size: 654 399
PciI A'CATG_T
Cuts at: 0 650 1053
Size: 650 403
PstI C_TGCA'G
Cuts at: 0 475 942 1053
Size: 475 467 111
Fragments arranged by size:
475 467 111
SalI G'TCGA_C
Cuts at: 0 368 1053
Size: 368 685
SfcI C'TryA_G
Cuts at: 0 180 471 938 1053
Size: 180 291 467 115
Fragments arranged by size:
467 291 180 115
SspI AAT'ATT
Cuts at: 0 1026 1053
Size: 1026 27
StuI AGG'CCT
Cuts at: 0 190 1053
Size: 190 863
StyI C'CwwG_G
Cuts at: 0 32 1053
Size: 32 1021
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 305 1053
Size: 305 748
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 597 629 1053
Size: 597 32 424
Fragments arranged by size:
597 424 32
XmnI GAAnn'nnTTC
Cuts at: 0 840 1053
Size: 840 213
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AloI AlwNI ApoI
BanI BanII BbvCI BclI BlpI BmrI BmtI BpmI
Bpu10I BsaWI BsaXI BseRI BseYI Bsp1286I BspMI BstEII
BtgI BtsI EaeI EciI Eco57MI EcoNI HaeII Hin4I
HincII HindIII KpnI MmeI MspA1I NheI NspI PciI
PstI SalI SfcI SspI StuI StyI TaqII TstI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BfrBI BglI BglII
Bme1580I BmgBI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoICRI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI MfeI MluI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI ZraI