Restriction map for
pF1KSDA1066
(Linear) (Six-base) MAPSORT of: KIAA1066.seq Check: 5931 from: 1 to: 4008
KIAA1066.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3978 4008
Size: 3978 30
AccI GT'mk_AC
Cuts at: 0 1159 1343 1708 4008
Size: 1159 184 365 2300
Fragments arranged by size:
2300 1159 365 184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 1710 1722 1806 3013 4008
Size: 433 1277 12 84 1207 995
Fragments arranged by size:
1277 1207 995 433 84 12
AflIII A'CryG_T
Cuts at: 0 626 1055 2255 3614 4008
Size: 626 429 1200 1359 394
Fragments arranged by size:
1359 1200 626 429 394
AhdI GACnn_n'nnGTC
Cuts at: 0 1011 1350 1902 4008
Size: 1011 339 552 2106
Fragments arranged by size:
2106 1011 552 339
AleI CACnn'nnGTG
Cuts at: 0 704 1573 2386 2542 2605 2698 3077 4008
Size: 704 869 813 156 63 93 379 931
Fragments arranged by size:
931 869 813 704 379 156 93 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 694 3725 4008
Size: 694 3031 283
Fragments arranged by size:
3031 694 283
AlwNI CAG_nnn'CTG
Cuts at: 0 882 1171 1290 2710 2833 3520 3546 3834
Size: 882 289 119 1420 123 687 26 288
Cuts at: 3834 4008
Size: 174
Fragments arranged by size:
1420 882 687 289 288 174 123 119
26
ApaI G_GGCC'C
Cuts at: 0 2717 3793 4008
Size: 2717 1076 215
Fragments arranged by size:
2717 1076 215
ApaLI G'TGCA_C
Cuts at: 0 656 2944 3199 4008
Size: 656 2288 255 809
Fragments arranged by size:
2288 809 656 255
ApoI r'AATT_y
Cuts at: 0 319 1879 4008
Size: 319 1560 2129
Fragments arranged by size:
2129 1560 319
AvaI C'yCGr_G
Cuts at: 0 923 1673 2263 2467 2508 2591 2746 3598
Size: 923 750 590 204 41 83 155 852
Cuts at: 3598 4003 4008
Size: 405 5
Fragments arranged by size:
923 852 750 590 405 204 155 83
41 5
AvrII C'CTAG_G
Cuts at: 0 1209 4008
Size: 1209 2799
BamHI G'GATC_C
Cuts at: 0 2321 4008
Size: 2321 1687
BanI G'GyrC_C
Cuts at: 0 698 808 2070 2700 3478 3589 3701 3732
Size: 698 110 1262 630 778 111 112 31
Cuts at: 3732 4008
Size: 276
Fragments arranged by size:
1262 778 698 630 276 112 111 110
31
BanII G_rGCy'C
Cuts at: 0 103 164 904 2243 2288 2717 2867 2925
Size: 103 61 740 1339 45 429 150 58
Cuts at: 2925 3386 3554 3793 4008
Size: 461 168 239 215
Fragments arranged by size:
1339 740 461 429 239 215 168 150
103 61 58 45
BbeI G_GCGC'C
Cuts at: 0 3593 4008
Size: 3593 415
BbsI GAAGACnn'nnnn_
Cuts at: 0 2760 4008
Size: 2760 1248
BbvCI CC'TCA_GC
Cuts at: 0 1372 4008
Size: 1372 2636
BclI T'GATC_A
Cuts at: 0 1661 2357 4008
Size: 1661 696 1651
Fragments arranged by size:
1661 1651 696
BglI GCCn_nnn'nGGC
Cuts at: 0 98 2689 4008
Size: 98 2591 1319
Fragments arranged by size:
2591 1319 98
BlpI GC'TnA_GC
Cuts at: 0 208 1394 3007 3853 4008
Size: 208 1186 1613 846 155
Fragments arranged by size:
1613 1186 846 208 155
Bme1580I G_kGCm'C
Cuts at: 0 660 842 924 1852 2383 2717 2948 3203
Size: 660 182 82 928 531 334 231 255
Cuts at: 3203 3481 3704 3793 4008
Size: 278 223 89 215
Fragments arranged by size:
928 660 531 334 278 255 231 223
215 182 89 82
BmgBI CAC'GTC
Cuts at: 0 1904 2258 2493 2766 3204 3372 4008
Size: 1904 354 235 273 438 168 636
Fragments arranged by size:
1904 636 438 354 273 235 168
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 2599 2661 4008
Size: 270 2329 62 1347
Fragments arranged by size:
2329 1347 270 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3559 4008
Size: 3559 449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 259 427 717 1734 2016 2292 2415
Size: 208 51 168 290 1017 282 276 123
Cuts at: 2415 4008
Size: 1593
Fragments arranged by size:
1593 1017 290 282 276 208 168 123
51
Bpu10I CC'TnA_GC
Cuts at: 0 1372 2143 4008
Size: 1372 771 1865
Fragments arranged by size:
1865 1372 771
BsaI GGTCTCn'nnnn_
Cuts at: 0 1060 4008
Size: 1060 2948
BsaAI yAC'GTr
Cuts at: 0 525 655 1959 2418 3615 4008
Size: 525 130 1304 459 1197 393
Fragments arranged by size:
1304 1197 525 459 393 130
BsaBI GATnn'nnATC
Cuts at: 0 7 4008
Size: 7 4001
BsaHI Gr'CG_yC
Cuts at: 0 2135 2300 2366 3245 3590 4008
Size: 2135 165 66 879 345 418
Fragments arranged by size:
2135 879 418 345 165 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 43 70 73 100 445 475 1123 1153
Size: 43 27 3 27 345 30 648 30
Cuts at: 1153 2290 2320 2572 2602 4008
Size: 1137 30 252 30 1406
Fragments arranged by size:
1406 1137 648 345 252 43 30 30
30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 328 466 2577 2692 3928 4008
Size: 160 168 138 2111 115 1236 80
Fragments arranged by size:
2111 1236 168 160 138 115 80
BseYI C'CCAG_C
Cuts at: 0 796 1553 2173 2716 2778 2804 2866 2916
Size: 796 757 620 543 62 26 62 50
Cuts at: 2916 3417 3683 3780 4008
Size: 501 266 97 228
Fragments arranged by size:
796 757 620 543 501 266 228 97
62 62 50 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1884 2885 2962 3514 4008
Size: 475 1409 1001 77 552 494
Fragments arranged by size:
1409 1001 552 494 475 77
BsiEI CG_ry'CG
Cuts at: 0 788 848 1977 2223 3171 4008
Size: 788 60 1129 246 948 837
Fragments arranged by size:
1129 948 837 788 246 60
BsiHKAI G_wGCw'C
Cuts at: 0 164 209 227 383 533 660 1595 1682
Size: 164 45 18 156 150 127 935 87
Cuts at: 1682 1958 2288 2765 2948 3203 4008
Size: 276 330 477 183 255 805
Fragments arranged by size:
935 805 477 330 276 255 183 164
156 150 127 87 45 18
BsiWI C'GTAC_G
Cuts at: 0 3432 4008
Size: 3432 576
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2225 2264 3884 3901 4008
Size: 2225 39 1620 17 107
Fragments arranged by size:
2225 1620 107 39 17
Bsp1286I G_dGCh'C
Cuts at: 0 103 164 209 227 383 533 660 842
Size: 103 61 45 18 156 150 127 182
Cuts at: 842 904 924 1595 1682 1852 1958 2243 2288
Size: 62 20 671 87 170 106 285 45
Cuts at: 2288 2383 2717 2765 2867 2925 2948 3203 3386
Size: 95 334 48 102 58 23 255 183
Cuts at: 3386 3481 3554 3704 3793 4008
Size: 95 73 150 89 215
Fragments arranged by size:
671 334 285 255 215 183 182 170
156 150 150 127 106 103 102 95
95 89 87 73 62 61 58 48
45 45 23 20 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 546 2224 3978 4008
Size: 546 1678 1754 30
Fragments arranged by size:
1754 1678 546 30
BsrBI CCG'CTC
Cuts at: 0 81 456 1602 1620 2147 3857 4008
Size: 81 375 1146 18 527 1710 151
Fragments arranged by size:
1710 1146 527 375 151 81 18
BsrDI GCAATG_nn'
Cuts at: 0 2737 4008
Size: 2737 1271
BsrFI r'CCGG_y
Cuts at: 0 1816 1868 2067 2073 2530 3464 3481 4008
Size: 1816 52 199 6 457 934 17 527
Fragments arranged by size:
1816 934 527 457 199 52 17 6
BsrGI T'GTAC_A
Cuts at: 0 1525 4008
Size: 1525 2483
BssSI C'ACGA_G
Cuts at: 0 226 676 4008
Size: 226 450 3332
Fragments arranged by size:
3332 450 226
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1368 4008
Size: 1368 2640
BstEII G'GTnAC_C
Cuts at: 0 960 4008
Size: 960 3048
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2352 2916 3318 3619 4008
Size: 2352 564 402 301 389
Fragments arranged by size:
2352 564 402 389 301
BstYI r'GATC_y
Cuts at: 0 8 2321 3096 4008
Size: 8 2313 775 912
Fragments arranged by size:
2313 912 775 8
Bsu36I CC'TnA_GG
Cuts at: 0 3331 4008
Size: 3331 677
BtgI C'CryG_G
Cuts at: 0 1547 2129 2441 2572 2693 2879 3075 3253
Size: 1547 582 312 131 121 186 196 178
Cuts at: 3253 3671 3696 4008
Size: 418 25 312
Fragments arranged by size:
1547 582 418 312 312 196 186 178
131 121 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1244 1458 2625 3308 3638 3722 4008
Size: 1244 214 1167 683 330 84 286
Fragments arranged by size:
1244 1167 683 330 286 214 84
BtsI GCAGTG_nn'
Cuts at: 0 132 1008 1811 2901 3639 3771 4008
Size: 132 876 803 1090 738 132 237
Fragments arranged by size:
1090 876 803 738 237 132 132
DraIII CAC_nnn'GTG
Cuts at: 0 231 2912 4008
Size: 231 2681 1096
Fragments arranged by size:
2681 1096 231
DrdI GACnn_nn'nnGTC
Cuts at: 0 1167 4008
Size: 1167 2841
EaeI y'GGCC_r
Cuts at: 0 714 785 845 1763 1974 2444 2528 2633
Size: 714 71 60 918 211 470 84 105
Cuts at: 2633 2851 2957 3062 3087 3644 3749 4008
Size: 218 106 105 25 557 105 259
Fragments arranged by size:
918 714 557 470 259 218 211 106
105 105 105 84 71 60 25
EagI C'GGCC_G
Cuts at: 0 785 845 1974 4008
Size: 785 60 1129 2034
Fragments arranged by size:
2034 1129 785 60
EarI CTCTTCn'nnn_
Cuts at: 0 1433 1490 1741 1749 4008
Size: 1433 57 251 8 2259
Fragments arranged by size:
2259 1433 251 57 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 325 612 1516 1899 3064 3304 4008
Size: 325 287 904 383 1165 240 704
Fragments arranged by size:
1165 904 704 383 325 287 240
EcoICRI GAG'CTC
Cuts at: 0 162 2286 4008
Size: 162 2124 1722
Fragments arranged by size:
2124 1722 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 259 427 433 717 1710 1722 1734
Size: 208 51 168 6 284 993 12 12
Cuts at: 1734 1806 2016 2292 2415 3013 4008
Size: 72 210 276 123 598 995
Fragments arranged by size:
995 993 598 284 276 210 208 168
123 72 51 12 12 6
EcoO109I rG'GnC_Cy
Cuts at: 0 2103 2505 2874 3056 3789 3790 4008
Size: 2103 402 369 182 733 1 218
Fragments arranged by size:
2103 733 402 369 218 182 1
EcoRI G'AATT_C
Cuts at: 0 1879 4008
Size: 1879 2129
FspI TGC'GCA
Cuts at: 0 301 976 4008
Size: 301 675 3032
Fragments arranged by size:
3032 675 301
HaeII r_GCGC'y
Cuts at: 0 126 298 612 1560 1773 1833 2197 2252
Size: 126 172 314 948 213 60 364 55
Cuts at: 2252 3593 4008
Size: 1341 415
Fragments arranged by size:
1341 948 415 364 314 213 172 126
60 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 70 75 102 445 477 745 777
Size: 43 27 5 27 343 32 268 32
Cuts at: 777 997 1029 1336 1368 1669 1701 1888 1920
Size: 220 32 307 32 301 32 187 32
Cuts at: 1920 2290 2322 3093 3125 3482 3514 4008
Size: 370 32 771 32 357 32 494
Fragments arranged by size:
771 494 370 357 343 307 301 268
220 187 43 32 32 32 32 32
32 32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 0 1160 1344 1709 2139 2658 4008
Size: 1160 184 365 430 519 1350
Fragments arranged by size:
1350 1160 519 430 365 184
HindIII A'AGCT_T
Cuts at: 0 1118 4008
Size: 1118 2890
KasI G'GCGC_C
Cuts at: 0 3589 4008
Size: 3589 419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 428 443 540 842 1364 4008
Size: 56 372 15 97 302 522 2644
Fragments arranged by size:
2644 522 372 302 97 56 15
MscI TGG'CCA
Cuts at: 0 2635 2959 3064 3089 3751 4008
Size: 2635 324 105 25 662 257
Fragments arranged by size:
2635 662 324 257 105 25
MslI CAynn'nnrTG
Cuts at: 0 508 704 1573 2053 2386 2542 2605 2698
Size: 508 196 869 480 333 156 63 93
Cuts at: 2698 3077 3613 3617 3707 3979 4008
Size: 379 536 4 90 272 29
Fragments arranged by size:
869 536 508 480 379 333 272 196
156 93 90 63 29 4
MspA1I CmG'CkG
Cuts at: 0 171 267 405 498 720 1350 2022 2131
Size: 171 96 138 93 222 630 672 109
Cuts at: 2131 2443 2561 2574 2870 3149 3255 3279 3802
Size: 312 118 13 296 279 106 24 523
Cuts at: 3802 4008
Size: 206
Fragments arranged by size:
672 630 523 312 296 279 222 206
171 138 118 109 106 96 93 24
13
NaeI GCC'GGC
Cuts at: 0 1818 1870 3466 4008
Size: 1818 52 1596 542
Fragments arranged by size:
1818 1596 542 52
NarI GG'CG_CC
Cuts at: 0 3590 4008
Size: 3590 418
NcoI C'CATG_G
Cuts at: 0 1547 3671 3696 4008
Size: 1547 2124 25 312
Fragments arranged by size:
2124 1547 312 25
NgoMIV G'CCGG_C
Cuts at: 0 1816 1868 3464 4008
Size: 1816 52 1596 544
Fragments arranged by size:
1816 1596 544 52
NotI GC'GGCC_GC
Cuts at: 0 785 4008
Size: 785 3223
NspI r_CATG'y
Cuts at: 0 1059 3022 4008
Size: 1059 1963 986
Fragments arranged by size:
1963 1059 986
PasI CC'CwG_GG
Cuts at: 0 882 1101 2710 3793 4008
Size: 882 219 1609 1083 215
Fragments arranged by size:
1609 1083 882 219 215
PciI A'CATG_T
Cuts at: 0 1055 4008
Size: 1055 2953
PflMI CCAn_nnn'nTGG
Cuts at: 0 2710 2912 3546 4008
Size: 2710 202 634 462
Fragments arranged by size:
2710 634 462 202
PfoI T'CCnGG_A
Cuts at: 0 1691 2480 4008
Size: 1691 789 1528
Fragments arranged by size:
1691 1528 789
PmlI CAC'GTG
Cuts at: 0 1959 2418 3615 4008
Size: 1959 459 1197 393
Fragments arranged by size:
1959 1197 459 393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 4008
Size: 184 32 3792
Fragments arranged by size:
3792 184 32
PpuMI rG'GwC_Cy
Cuts at: 0 2103 2505 2874 3056 4008
Size: 2103 402 369 182 952
Fragments arranged by size:
2103 952 402 369 182
PshAI GACnn'nnGTC
Cuts at: 0 1058 1976 3523 4008
Size: 1058 918 1547 485
Fragments arranged by size:
1547 1058 918 485
PspOMI G'GGCC_C
Cuts at: 0 2713 3789 4008
Size: 2713 1076 219
Fragments arranged by size:
2713 1076 219
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1780 1812 4008
Size: 1780 32 2196
Fragments arranged by size:
2196 1780 32
PstI C_TGCA'G
Cuts at: 0 725 878 1637 1985 4008
Size: 725 153 759 348 2023
Fragments arranged by size:
2023 759 725 348 153
PvuII CAG'CTG
Cuts at: 0 267 405 720 1350 2022 3279 3802 4008
Size: 267 138 315 630 672 1257 523 206
Fragments arranged by size:
1257 672 630 523 315 267 206 138
SacI G_AGCT'C
Cuts at: 0 164 2288 4008
Size: 164 2124 1720
Fragments arranged by size:
2124 1720 164
SacII CC_GC'GG
Cuts at: 0 2132 2444 2575 3256 4008
Size: 2132 312 131 681 752
Fragments arranged by size:
2132 752 681 312 131
SalI G'TCGA_C
Cuts at: 0 1158 1342 1707 4008
Size: 1158 184 365 2301
Fragments arranged by size:
2301 1158 365 184
SanDI GG'GwC_CC
Cuts at: 0 2874 3056 4008
Size: 2874 182 952
Fragments arranged by size:
2874 952 182
SapI GCTCTTCn'nnn_
Cuts at: 0 1749 4008
Size: 1749 2259
SfcI C'TryA_G
Cuts at: 0 721 874 1633 1981 3364 4008
Size: 721 153 759 348 1383 644
Fragments arranged by size:
1383 759 721 644 348 153
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2689 4008
Size: 2689 1319
SfoI GGC'GCC
Cuts at: 0 3591 4008
Size: 3591 417
SmaI CCC'GGG
Cuts at: 0 925 2469 2510 2748 3600 4008
Size: 925 1544 41 238 852 408
Fragments arranged by size:
1544 925 852 408 238 41
SrfI GCCC'GGGC
Cuts at: 0 2748 4008
Size: 2748 1260
StuI AGG'CCT
Cuts at: 0 1987 4008
Size: 1987 2021
StyI C'CwwG_G
Cuts at: 0 1209 1337 1547 2279 3671 3696 3956 4008
Size: 1209 128 210 732 1392 25 260 52
Fragments arranged by size:
1392 1209 732 260 210 128 52 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 289 592 810 979 1697 1821 2237 2919
Size: 289 303 218 169 718 124 416 682
Cuts at: 2919 3294 3462 4008
Size: 375 168 546
Fragments arranged by size:
718 682 546 416 375 303 289 218
169 168 124
TatI w'GTAC_w
Cuts at: 0 479 954 1525 1610 2012 2078 4008
Size: 479 475 571 85 402 66 1930
Fragments arranged by size:
1930 571 479 475 402 85 66
Tth111I GACn'n_nGTC
Cuts at: 0 2790 4008
Size: 2790 1218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1047 1410 2349 2913 3150 4008
Size: 1047 363 939 564 237 858
Fragments arranged by size:
1047 939 858 564 363 237
XmaI C'CCGG_G
Cuts at: 0 923 2467 2508 2746 3598 4008
Size: 923 1544 41 238 852 410
Fragments arranged by size:
1544 923 852 410 238 41
XmnI GAAnn'nnTTC
Cuts at: 0 628 4008
Size: 628 3380
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BclI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuII SacI SacII SalI
SanDI SapI SfcI SfiI SfoI SmaI SrfI StuI
StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI AscI
AseI AsiSI BaeI BcgI BciVI BfrBI BglII BmtI
BpuEI BsaWI BsmI BspEI BspHI BssHII BstBI BstZ17I
ClaI CspCI DraI EcoNI EcoRV FalI FseI FspAI
HpaI KpnI MfeI MluI NdeI NheI NruI NsiI
PacI PmeI PsiI PspXI PvuI RsrII SbfI ScaI
SexAI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
SwaI TstI XbaI XhoI ZraI