Restriction map for
pF1KSDA1059
(Linear) (Six-base) MAPSORT of: KIAA1059.seq Check: 4201 from: 1 to: 3666
KIAA1059.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 294 1117 1661 2123 3666
Size: 294 823 544 462 1543
Fragments arranged by size:
1543 823 544 462 294
AccI GT'mk_AC
Cuts at: 0 76 697 3666
Size: 76 621 2969
Fragments arranged by size:
2969 621 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 2007 2058 2598 3146 3310 3666
Size: 554 1453 51 540 548 164 356
Fragments arranged by size:
1453 554 548 540 356 164 51
AflIII A'CryG_T
Cuts at: 0 1241 1370 1396 2642 3666
Size: 1241 129 26 1246 1024
Fragments arranged by size:
1246 1241 1024 129 26
AhdI GACnn_n'nnGTC
Cuts at: 0 122 3666
Size: 122 3544
AlwNI CAG_nnn'CTG
Cuts at: 0 364 897 3666
Size: 364 533 2769
Fragments arranged by size:
2769 533 364
ApaLI G'TGCA_C
Cuts at: 0 601 1063 1615 3666
Size: 601 462 552 2051
Fragments arranged by size:
2051 601 552 462
ApoI r'AATT_y
Cuts at: 0 3270 3666
Size: 3270 396
AvaI C'yCGr_G
Cuts at: 0 491 2462 3666
Size: 491 1971 1204
Fragments arranged by size:
1971 1204 491
BamHI G'GATC_C
Cuts at: 0 1115 3666
Size: 1115 2551
BanI G'GyrC_C
Cuts at: 0 42 94 245 507 709 1571 1852 2111
Size: 42 52 151 262 202 862 281 259
Cuts at: 2111 3666
Size: 1555
Fragments arranged by size:
1555 862 281 262 259 202 151 52
42
BanII G_rGCy'C
Cuts at: 0 65 1751 1974 2184 3096 3666
Size: 65 1686 223 210 912 570
Fragments arranged by size:
1686 912 570 223 210 65
BbeI G_GCGC'C
Cuts at: 0 46 98 249 511 1575 3666
Size: 46 52 151 262 1064 2091
Fragments arranged by size:
2091 1064 262 151 52 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 542 754 984 1543 3666
Size: 542 212 230 559 2123
Fragments arranged by size:
2123 559 542 230 212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 543 577 3666
Size: 543 34 3089
Fragments arranged by size:
3089 543 34
BclI T'GATC_A
Cuts at: 0 836 3666
Size: 836 2830
BfrBI ATG'CAT
Cuts at: 0 996 3666
Size: 996 2670
BglI GCCn_nnn'nGGC
Cuts at: 0 29 1861 1890 3666
Size: 29 1832 29 1776
Fragments arranged by size:
1832 1776 29 29
BglII A'GATC_T
Cuts at: 0 1808 3666
Size: 1808 1858
BlpI GC'TnA_GC
Cuts at: 0 3372 3666
Size: 3372 294
Bme1580I G_kGCm'C
Cuts at: 0 605 1067 1619 1857 2193 2449 2516 3365
Size: 605 462 552 238 336 256 67 849
Cuts at: 3365 3666
Size: 301
Fragments arranged by size:
849 605 552 462 336 301 256 238
67
BmgBI CAC'GTC
Cuts at: 0 80 3666
Size: 80 3586
BmrI ACTGGGnnnn_n'
Cuts at: 0 933 1606 1854 2381 2810 2833 3666
Size: 933 673 248 527 429 23 833
Fragments arranged by size:
933 833 673 527 429 248 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1938 3666
Size: 1938 1728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3336 3666
Size: 3336 330
Bpu10I CC'TnA_GC
Cuts at: 0 482 2413 3666
Size: 482 1931 1253
Fragments arranged by size:
1931 1253 482
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3455 3666
Size: 3455 211
BsaI GGTCTCn'nnnn_
Cuts at: 0 1987 2195 2226 3157 3666
Size: 1987 208 31 931 509
Fragments arranged by size:
1987 931 509 208 31
BsaAI yAC'GTr
Cuts at: 0 1242 1373 2643 3666
Size: 1242 131 1270 1023
Fragments arranged by size:
1270 1242 1023 131
BsaBI GATnn'nnATC
Cuts at: 0 3460 3666
Size: 3460 206
BsaHI Gr'CG_yC
Cuts at: 0 43 95 132 217 246 431 508 1572
Size: 43 52 37 85 29 185 77 1064
Cuts at: 1572 3666
Size: 2094
Fragments arranged by size:
2094 1064 185 85 77 52 43 37
29
BsaWI w'CCGG_w
Cuts at: 0 53 1587 3666
Size: 53 1534 2079
Fragments arranged by size:
2079 1534 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 202 232 2278 2308 3321 3351 3666
Size: 202 30 2046 30 1013 30 315
Fragments arranged by size:
2046 1013 315 202 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 399 402 511 1770 2557 3219 3580
Size: 223 176 3 109 1259 787 662 361
Cuts at: 3580 3666
Size: 86
Fragments arranged by size:
1259 787 662 361 223 176 109 86
3
BseYI C'CCAG_C
Cuts at: 0 240 272 357 517 550 2301 2947 3005
Size: 240 32 85 160 33 1751 646 58
Cuts at: 3005 3358 3666
Size: 353 308
Fragments arranged by size:
1751 646 353 308 240 160 85 58
33 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1633 2385 2822 3639 3666
Size: 1633 752 437 817 27
Fragments arranged by size:
1633 817 752 437 27
BsiEI CG_ry'CG
Cuts at: 0 452 2486 3666
Size: 452 2034 1180
Fragments arranged by size:
2034 1180 452
BsiHKAI G_wGCw'C
Cuts at: 0 605 1067 1619 1751 2184 3096 3666
Size: 605 462 552 132 433 912 570
Fragments arranged by size:
912 605 570 552 462 433 132
BsmI GAATG_Cn'
Cuts at: 0 2958 3666
Size: 2958 708
Bsp1286I G_dGCh'C
Cuts at: 0 65 605 1067 1619 1751 1857 1974 2184
Size: 65 540 462 552 132 106 117 210
Cuts at: 2184 2193 2449 2516 3096 3365 3666
Size: 9 256 67 580 269 301
Fragments arranged by size:
580 552 540 462 301 269 256 210
132 117 106 67 65 9
BspEI T'CCGG_A
Cuts at: 0 53 1587 3666
Size: 53 1534 2079
Fragments arranged by size:
2079 1534 53
BspHI T'CATG_A
Cuts at: 0 2003 2988 3666
Size: 2003 985 678
Fragments arranged by size:
2003 985 678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 294 1117 1477 1661 2123 3666
Size: 294 823 360 184 462 1543
Fragments arranged by size:
1543 823 462 360 294 184
BsrBI CCG'CTC
Cuts at: 0 339 1160 2033 3666
Size: 339 821 873 1633
Fragments arranged by size:
1633 873 821 339
BsrDI GCAATG_nn'
Cuts at: 0 2082 3084 3134 3222 3608 3666
Size: 2082 1002 50 88 386 58
Fragments arranged by size:
2082 1002 386 88 58 50
BsrFI r'CCGG_y
Cuts at: 0 91 159 327 2078 3666
Size: 91 68 168 1751 1588
Fragments arranged by size:
1751 1588 168 91 68
BssHII G'CGCG_C
Cuts at: 0 10 3666
Size: 10 3656
BssSI C'ACGA_G
Cuts at: 0 1858 3666
Size: 1858 1808
BstAPI GCAn_nnn'nTGC
Cuts at: 0 993 1623 3666
Size: 993 630 2043
Fragments arranged by size:
2043 993 630
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2309 2823 3666
Size: 2309 514 843
Fragments arranged by size:
2309 843 514
BstYI r'GATC_y
Cuts at: 0 1115 1808 2660 3128 3644 3666
Size: 1115 693 852 468 516 22
Fragments arranged by size:
1115 852 693 516 468 22
Bsu36I CC'TnA_GG
Cuts at: 0 1680 3563 3666
Size: 1680 1883 103
Fragments arranged by size:
1883 1680 103
BtgI C'CryG_G
Cuts at: 0 181 511 596 965 1082 1421 3666
Size: 181 330 85 369 117 339 2245
Fragments arranged by size:
2245 369 339 330 181 117 85
BtsI GCAGTG_nn'
Cuts at: 0 194 440 1617 3666
Size: 194 246 1177 2049
Fragments arranged by size:
2049 1177 246 194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 146 181 3666
Size: 146 35 3485
Fragments arranged by size:
3485 146 35
DraI TTT'AAA
Cuts at: 0 3438 3666
Size: 3438 228
DraIII CAC_nnn'GTG
Cuts at: 0 3547 3666
Size: 3547 119
DrdI GACnn_nn'nnGTC
Cuts at: 0 2710 3595 3666
Size: 2710 885 71
Fragments arranged by size:
2710 885 71
EaeI y'GGCC_r
Cuts at: 0 89 154 191 202 449 1034 1418 1862
Size: 89 65 37 11 247 585 384 444
Cuts at: 1862 2016 3666
Size: 154 1650
Fragments arranged by size:
1650 585 444 384 247 154 89 65
37 11
EagI C'GGCC_G
Cuts at: 0 449 3666
Size: 449 3217
EarI CTCTTCn'nnn_
Cuts at: 0 1221 1248 1814 2722 3666
Size: 1221 27 566 908 944
Fragments arranged by size:
1221 944 908 566 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1069 2397 3666
Size: 1069 1328 1269
Fragments arranged by size:
1328 1269 1069
EcoICRI GAG'CTC
Cuts at: 0 1749 2182 3094 3666
Size: 1749 433 912 572
Fragments arranged by size:
1749 912 572 433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 2007 2058 2598 3146 3310 3336 3666
Size: 554 1453 51 540 548 164 26 330
Fragments arranged by size:
1453 554 548 540 330 164 51 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 481 2539 2962 3666
Size: 481 2058 423 704
Fragments arranged by size:
2058 704 481 423
EcoO109I rG'GnC_Cy
Cuts at: 0 84 2466 2828 3225 3666
Size: 84 2382 362 397 441
Fragments arranged by size:
2382 441 397 362 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1166 3666
Size: 1166 2500
HaeII r_GCGC'y
Cuts at: 0 24 46 98 249 511 1575 3666
Size: 24 22 52 151 262 1064 2091
Fragments arranged by size:
2091 1064 262 151 52 24 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 202 234 375 407 2050 2082
Size: 108 32 62 32 141 32 1643 32
Cuts at: 2082 2204 2236 3321 3353 3666
Size: 122 32 1085 32 313
Fragments arranged by size:
1643 1085 313 141 122 108 62 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 77 698 1965 2793 2821 3666
Size: 77 621 1267 828 28 845
Fragments arranged by size:
1267 845 828 621 77 28
HindIII A'AGCT_T
Cuts at: 0 370 3666
Size: 370 3296
KasI G'GCGC_C
Cuts at: 0 42 94 245 507 1571 3666
Size: 42 52 151 262 1064 2095
Fragments arranged by size:
2095 1064 262 151 52 42
MfeI C'AATT_G
Cuts at: 0 2044 2244 3666
Size: 2044 200 1422
Fragments arranged by size:
2044 1422 200
MluI A'CGCG_T
Cuts at: 0 1396 3666
Size: 1396 2270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 2417 3501 3666
Size: 802 1615 1084 165
Fragments arranged by size:
1615 1084 802 165
MscI TGG'CCA
Cuts at: 0 193 1420 1864 2018 3666
Size: 193 1227 444 154 1648
Fragments arranged by size:
1648 1227 444 193 154
MslI CAynn'nnrTG
Cuts at: 0 1072 2536 3139 3382 3666
Size: 1072 1464 603 243 284
Fragments arranged by size:
1464 1072 603 284 243
MspA1I CmG'CkG
Cuts at: 0 183 513 2710 3327 3666
Size: 183 330 2197 617 339
Fragments arranged by size:
2197 617 339 330 183
NaeI GCC'GGC
Cuts at: 0 93 2080 3666
Size: 93 1987 1586
Fragments arranged by size:
1987 1586 93
NarI GG'CG_CC
Cuts at: 0 43 95 246 508 1572 3666
Size: 43 52 151 262 1064 2094
Fragments arranged by size:
2094 1064 262 151 52 43
NcoI C'CATG_G
Cuts at: 0 596 965 1421 3666
Size: 596 369 456 2245
Fragments arranged by size:
2245 596 456 369
NgoMIV G'CCGG_C
Cuts at: 0 91 2078 3666
Size: 91 1987 1588
Fragments arranged by size:
1987 1588 91
NsiI A_TGCA'T
Cuts at: 0 998 3666
Size: 998 2668
NspI r_CATG'y
Cuts at: 0 1308 2728 2749 3666
Size: 1308 1420 21 917
Fragments arranged by size:
1420 1308 917 21
PasI CC'CwG_GG
Cuts at: 0 2193 3666
Size: 2193 1473
PflMI CCAn_nnn'nTGG
Cuts at: 0 200 597 3273 3666
Size: 200 397 2676 393
Fragments arranged by size:
2676 397 393 200
PfoI T'CCnGG_A
Cuts at: 0 126 1118 1187 2963 3666
Size: 126 992 69 1776 703
Fragments arranged by size:
1776 992 703 126 69
PmlI CAC'GTG
Cuts at: 0 2643 3666
Size: 2643 1023
PpuMI rG'GwC_Cy
Cuts at: 0 84 2828 3225 3666
Size: 84 2744 397 441
Fragments arranged by size:
2744 441 397 84
PshAI GACnn'nnGTC
Cuts at: 0 82 3666
Size: 82 3584
PstI C_TGCA'G
Cuts at: 0 1114 2344 3666
Size: 1114 1230 1322
Fragments arranged by size:
1322 1230 1114
PvuII CAG'CTG
Cuts at: 0 2710 3327 3666
Size: 2710 617 339
Fragments arranged by size:
2710 617 339
SacI G_AGCT'C
Cuts at: 0 1751 2184 3096 3666
Size: 1751 433 912 570
Fragments arranged by size:
1751 912 570 433
SacII CC_GC'GG
Cuts at: 0 184 514 3666
Size: 184 330 3152
Fragments arranged by size:
3152 330 184
SalI G'TCGA_C
Cuts at: 0 75 696 3666
Size: 75 621 2970
Fragments arranged by size:
2970 621 75
SanDI GG'GwC_CC
Cuts at: 0 2828 3666
Size: 2828 838
SapI GCTCTTCn'nnn_
Cuts at: 0 1221 3666
Size: 1221 2445
SbfI CC_TGCA'GG
Cuts at: 0 1114 3666
Size: 1114 2552
ScaI AGT'ACT
Cuts at: 0 1288 3666
Size: 1288 2378
SexAI A'CCwGG_T
Cuts at: 0 1058 1529 3666
Size: 1058 471 2137
Fragments arranged by size:
2137 1058 471
SfcI C'TryA_G
Cuts at: 0 936 957 1110 1727 2340 3666
Size: 936 21 153 617 613 1326
Fragments arranged by size:
1326 936 617 613 153 21
SfoI GGC'GCC
Cuts at: 0 44 96 247 509 1573 3666
Size: 44 52 151 262 1064 2093
Fragments arranged by size:
2093 1064 262 151 52 44
SgrAI Cr'CCGG_yG
Cuts at: 0 159 3666
Size: 159 3507
SmaI CCC'GGG
Cuts at: 0 493 3666
Size: 493 3173
SmlI C'TyrA_G
Cuts at: 0 3470 3666
Size: 3470 196
SpeI A'CTAG_T
Cuts at: 0 1219 3658 3666
Size: 1219 2439 8
Fragments arranged by size:
2439 1219 8
SspI AAT'ATT
Cuts at: 0 2159 2995 3666
Size: 2159 836 671
Fragments arranged by size:
2159 836 671
StuI AGG'CCT
Cuts at: 0 1261 3666
Size: 1261 2405
StyI C'CwwG_G
Cuts at: 0 596 965 1421 2090 2346 2432 2694 3451
Size: 596 369 456 669 256 86 262 757
Cuts at: 3451 3666
Size: 215
Fragments arranged by size:
757 669 596 456 369 262 256 215
86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 915 1026 1890 2500 3201 3666
Size: 430 485 111 864 610 701 465
Fragments arranged by size:
864 701 610 485 465 430 111
TatI w'GTAC_w
Cuts at: 0 1286 1318 1823 2423 3666
Size: 1286 32 505 600 1243
Fragments arranged by size:
1286 1243 600 505 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1598 1630 1706 1738 1935 1967 2242 2274
Size: 1598 32 76 32 197 32 275 32
Cuts at: 2274 2278 2310 3666
Size: 4 32 1356
Fragments arranged by size:
1598 1356 275 197 76 32 32 32
32 32 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2446 2578 2944 3666
Size: 2446 132 366 722
Fragments arranged by size:
2446 722 366 132
XmaI C'CCGG_G
Cuts at: 0 491 3666
Size: 491 3175
XmnI GAAnn'nnTTC
Cuts at: 0 2158 3666
Size: 2158 1508
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BcgI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PstI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgrAI SmaI
SmlI SpeI SspI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BbvCI
BciVI BmtI BsiWI BsmBI BsrGI BstBI BstEII BstZ17I
BtgZI ClaI EcoRI EcoRV FseI FspI FspAI HpaI
KpnI NdeI NheI NotI NruI PacI PciI PmeI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SfiI
SgfI SnaBI SphI SrfI SwaI Tth111I XbaI XhoI
ZraI