Restriction map for
pF1KA0970
(Linear) (Six-base) MAPSORT of: KIAA0970.seq Check: 3863 from: 1 to: 3426
KIAA0970.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 1848 3426
Size: 1848 1578
AclI AA'CG_TT
Cuts at: 0 384 3426
Size: 384 3042
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1343 1526 2000 2013 2779 3139 3426
Size: 890 453 183 474 13 766 360 287
Fragments arranged by size:
890 766 474 453 360 287 183 13
AflII C'TTAA_G
Cuts at: 0 2731 3426
Size: 2731 695
AflIII A'CryG_T
Cuts at: 0 2134 3426
Size: 2134 1292
AleI CACnn'nnGTG
Cuts at: 0 3017 3250 3426
Size: 3017 233 176
Fragments arranged by size:
3017 233 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3268 3368 3426
Size: 3268 100 58
Fragments arranged by size:
3268 100 58
AlwNI CAG_nnn'CTG
Cuts at: 0 913 2098 2325 2389 3426
Size: 913 1185 227 64 1037
Fragments arranged by size:
1185 1037 913 227 64
ApaLI G'TGCA_C
Cuts at: 0 2153 3426
Size: 2153 1273
ApoI r'AATT_y
Cuts at: 0 984 1078 1150 1325 1441 1498 2612 2890
Size: 984 94 72 175 116 57 1114 278
Cuts at: 2890 3103 3426
Size: 213 323
Fragments arranged by size:
1114 984 323 278 213 175 116 94
72 57
AseI AT'TA_AT
Cuts at: 0 476 710 3426
Size: 476 234 2716
Fragments arranged by size:
2716 476 234
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 557 590 3426
Size: 557 33 2836
Fragments arranged by size:
2836 557 33
BamHI G'GATC_C
Cuts at: 0 1587 3426
Size: 1587 1839
BanI G'GyrC_C
Cuts at: 0 129 1008 3426
Size: 129 879 2418
Fragments arranged by size:
2418 879 129
BanII G_rGCy'C
Cuts at: 0 746 1102 3426
Size: 746 356 2324
Fragments arranged by size:
2324 746 356
BbsI GAAGACnn'nnnn_
Cuts at: 0 98 290 2398 3067 3426
Size: 98 192 2108 669 359
Fragments arranged by size:
2108 669 359 192 98
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1730 1764 3331 3365 3426
Size: 1730 34 1567 34 61
Fragments arranged by size:
1730 1567 61 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2835 3426
Size: 2835 591
BclI T'GATC_A
Cuts at: 0 2490 3009 3426
Size: 2490 519 417
Fragments arranged by size:
2490 519 417
BglII A'GATC_T
Cuts at: 0 1395 2140 3426
Size: 1395 745 1286
Fragments arranged by size:
1395 1286 745
Bme1580I G_kGCm'C
Cuts at: 0 2157 3333 3426
Size: 2157 1176 93
Fragments arranged by size:
2157 1176 93
BmgBI CAC'GTC
Cuts at: 0 232 3426
Size: 232 3194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 281 1787 2233 3426
Size: 275 6 1506 446 1193
Fragments arranged by size:
1506 1193 446 275 6
Bpu10I CC'TnA_GC
Cuts at: 0 14 3426
Size: 14 3412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 753 826 951 2111 3426
Size: 753 73 125 1160 1315
Fragments arranged by size:
1315 1160 753 125 73
BsaI GGTCTCn'nnnn_
Cuts at: 0 1782 2269 3426
Size: 1782 487 1157
Fragments arranged by size:
1782 1157 487
BsaAI yAC'GTr
Cuts at: 0 2038 3426
Size: 2038 1388
BsaWI w'CCGG_w
Cuts at: 0 3140 3426
Size: 3140 286
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 173 2237 3426
Size: 173 2064 1189
Fragments arranged by size:
2064 1189 173
BseYI C'CCAG_C
Cuts at: 0 2474 2917 2970 3426
Size: 2474 443 53 456
Fragments arranged by size:
2474 456 443 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1816 2060 2360 3426
Size: 1816 244 300 1066
Fragments arranged by size:
1816 1066 300 244
BsiHKAI G_wGCw'C
Cuts at: 0 746 1230 2157 3257 3426
Size: 746 484 927 1100 169
Fragments arranged by size:
1100 927 746 484 169
Bsp1286I G_dGCh'C
Cuts at: 0 746 1102 1230 2157 3257 3333 3426
Size: 746 356 128 927 1100 76 93
Fragments arranged by size:
1100 927 746 356 128 93 76
BsrDI GCAATG_nn'
Cuts at: 0 1006 1145 3426
Size: 1006 139 2281
Fragments arranged by size:
2281 1006 139
BsrGI T'GTAC_A
Cuts at: 0 1990 3426
Size: 1990 1436
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2152 3426
Size: 2152 1274
BstBI TT'CG_AA
Cuts at: 0 105 3426
Size: 105 3321
BstEII G'GTnAC_C
Cuts at: 0 689 3426
Size: 689 2737
BstYI r'GATC_y
Cuts at: 0 1395 1587 2140 3426
Size: 1395 192 553 1286
Fragments arranged by size:
1395 1286 553 192
Bsu36I CC'TnA_GG
Cuts at: 0 125 1218 1302 3426
Size: 125 1093 84 2124
Fragments arranged by size:
2124 1093 125 84
BtgI C'CryG_G
Cuts at: 0 851 3426
Size: 851 2575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2435 3426
Size: 2435 991
BtsI GCAGTG_nn'
Cuts at: 0 3352 3426
Size: 3352 74
DraI TTT'AAA
Cuts at: 0 833 1116 1575 3426
Size: 833 283 459 1851
Fragments arranged by size:
1851 833 459 283
DraIII CAC_nnn'GTG
Cuts at: 0 1015 3337 3426
Size: 1015 2322 89
Fragments arranged by size:
2322 1015 89
EaeI y'GGCC_r
Cuts at: 0 1163 3426
Size: 1163 2263
EarI CTCTTCn'nnn_
Cuts at: 0 102 3138 3262 3426
Size: 102 3036 124 164
Fragments arranged by size:
3036 164 124 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1622 3426
Size: 1622 1804
EcoICRI GAG'CTC
Cuts at: 0 744 3426
Size: 744 2682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 281 890 1343 1526 1787 2000 2013
Size: 275 6 609 453 183 261 213 13
Cuts at: 2013 2233 2779 3139 3426
Size: 220 546 360 287
Fragments arranged by size:
609 546 453 360 287 275 261 220
213 183 13 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1882 2344 2926 3124 3225 3235 3320 3426
Size: 1882 462 582 198 101 10 85 106
Fragments arranged by size:
1882 582 462 198 106 101 85 10
EcoRI G'AATT_C
Cuts at: 0 2612 3103 3426
Size: 2612 491 323
Fragments arranged by size:
2612 491 323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 643 3426
Size: 643 2783
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 207 239 313 345 349 381 957 989
Size: 207 32 74 32 4 32 576 32
Cuts at: 989 1601 1633 1888 1920 2421 2453 2524 2556
Size: 612 32 255 32 501 32 71 32
Cuts at: 2556 2866 2898 3426
Size: 310 32 528
Fragments arranged by size:
612 576 528 501 310 255 207 74
71 32 32 32 32 32 32 32
32 32 4
HincII GTy'rAC
Cuts at: 0 2771 2879 3426
Size: 2771 108 547
Fragments arranged by size:
2771 547 108
HindIII A'AGCT_T
Cuts at: 0 2903 3426
Size: 2903 523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 656 680 3426
Size: 371 285 24 2746
Fragments arranged by size:
2746 371 285 24
MslI CAynn'nnrTG
Cuts at: 0 1703 3017 3033 3250 3364 3426
Size: 1703 1314 16 217 114 62
Fragments arranged by size:
1703 1314 217 114 62 16
MspA1I CmG'CkG
Cuts at: 0 1807 2974 3159 3426
Size: 1807 1167 185 267
Fragments arranged by size:
1807 1167 267 185
NcoI C'CATG_G
Cuts at: 0 851 3426
Size: 851 2575
NdeI CA'TA_TG
Cuts at: 0 236 378 2654 3426
Size: 236 142 2276 772
Fragments arranged by size:
2276 772 236 142
NspI r_CATG'y
Cuts at: 0 2138 2462 3426
Size: 2138 324 964
Fragments arranged by size:
2138 964 324
PasI CC'CwG_GG
Cuts at: 0 2098 3426
Size: 2098 1328
PciI A'CATG_T
Cuts at: 0 2134 3426
Size: 2134 1292
PfoI T'CCnGG_A
Cuts at: 0 66 2010 3426
Size: 66 1944 1416
Fragments arranged by size:
1944 1416 66
PpuMI rG'GwC_Cy
Cuts at: 0 1882 2926 3124 3225 3235 3320 3426
Size: 1882 1044 198 101 10 85 106
Fragments arranged by size:
1882 1044 198 106 101 85 10
PsiI TTA'TAA
Cuts at: 0 496 3426
Size: 496 2930
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 932 964 3426
Size: 932 32 2462
Fragments arranged by size:
2462 932 32
PvuII CAG'CTG
Cuts at: 0 1807 2974 3159 3426
Size: 1807 1167 185 267
Fragments arranged by size:
1807 1167 267 185
SacI G_AGCT'C
Cuts at: 0 746 3426
Size: 746 2680
SanDI GG'GwC_CC
Cuts at: 0 1882 3124 3426
Size: 1882 1242 302
Fragments arranged by size:
1882 1242 302
SapI GCTCTTCn'nnn_
Cuts at: 0 3262 3426
Size: 3262 164
ScaI AGT'ACT
Cuts at: 0 680 774 1431 3412 3426
Size: 680 94 657 1981 14
Fragments arranged by size:
1981 680 657 94 14
SexAI A'CCwGG_T
Cuts at: 0 189 685 3426
Size: 189 496 2741
Fragments arranged by size:
2741 496 189
SfcI C'TryA_G
Cuts at: 0 1940 2090 2514 3240 3426
Size: 1940 150 424 726 186
Fragments arranged by size:
1940 726 424 186 150
SmlI C'TyrA_G
Cuts at: 0 768 841 930 2126 2731 3426
Size: 768 73 89 1196 605 695
Fragments arranged by size:
1196 768 695 605 89 73
StyI C'CwwG_G
Cuts at: 0 411 851 961 1259 1971 2631 3426
Size: 411 440 110 298 712 660 795
Fragments arranged by size:
795 712 660 440 411 298 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1923 2326 3426
Size: 1923 403 1100
Fragments arranged by size:
1923 1100 403
TatI w'GTAC_w
Cuts at: 0 239 678 772 1429 1990 2859 3410 3426
Size: 239 439 94 657 561 869 551 16
Fragments arranged by size:
869 657 561 551 439 239 94 16
Tth111I GACn'n_nGTC
Cuts at: 0 2766 2843 3426
Size: 2766 77 583
Fragments arranged by size:
2766 583 77
XmnI GAAnn'nnTTC
Cuts at: 0 623 903 3096 3426
Size: 623 280 2193 330
Fragments arranged by size:
2193 623 330 280
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AleI AlfI AlwNI
ApaLI ApoI AseI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglII Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BsrDI BsrGI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII
MmeI MslI MspA1I NcoI NdeI NspI PasI PciI
PfoI PpuMI PsiI PsrI PvuII SacI SanDI SapI
ScaI SexAI SfcI SmlI StyI TaqII TatI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AhdI AloI ApaI
AscI AsiSI AvaI AvrII BbeI BbvCI BfrBI BglI
BlpI BmrI BmtI BplI BsaBI BsaHI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstXI BstZ17I ClaI CspCI DrdI EagI
EcoNI EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PpiI
PshAI PspOMI PspXI PstI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI XbaI XcmI
XhoI XmaI ZraI