Restriction map for
pF1KSDA0939
(Linear) (Six-base) MAPSORT of: KIAA0939.seq Check: 4866 from: 1 to: 1743
KIAA0939.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AatII G_ACGT'C
Cuts at: 0 1466 1743
Size: 1466 277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 740 877 1743
Size: 174 566 137 866
Fragments arranged by size:
866 566 174 137
AfeI AGC'GCT
Cuts at: 0 818 1743
Size: 818 925
AflII C'TTAA_G
Cuts at: 0 1570 1743
Size: 1570 173
AflIII A'CryG_T
Cuts at: 0 371 1078 1743
Size: 371 707 665
Fragments arranged by size:
707 665 371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1417 1743
Size: 1417 326
AlwNI CAG_nnn'CTG
Cuts at: 0 1737 1743
Size: 1737 6
ApaI G_GGCC'C
Cuts at: 0 1708 1743
Size: 1708 35
ApoI r'AATT_y
Cuts at: 0 515 1133 1212 1743
Size: 515 618 79 531
Fragments arranged by size:
618 531 515 79
BanI G'GyrC_C
Cuts at: 0 1345 1743
Size: 1345 398
BanII G_rGCy'C
Cuts at: 0 1322 1526 1708 1743
Size: 1322 204 182 35
Fragments arranged by size:
1322 204 182 35
BbvCI CC'TCA_GC
Cuts at: 0 1471 1743
Size: 1471 272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 81 115 1743
Size: 81 34 1628
Fragments arranged by size:
1628 81 34
BclI T'GATC_A
Cuts at: 0 905 1743
Size: 905 838
BglI GCCn_nnn'nGGC
Cuts at: 0 930 1712 1743
Size: 930 782 31
Fragments arranged by size:
930 782 31
Bme1580I G_kGCm'C
Cuts at: 0 1708 1743
Size: 1708 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 1015 1743
Size: 1015 728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1743
Size: 1336 407
Bpu10I CC'TnA_GC
Cuts at: 0 1297 1471 1743
Size: 1297 174 272
Fragments arranged by size:
1297 272 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 1743
Size: 891 852
BsaBI GATnn'nnATC
Cuts at: 0 1252 1743
Size: 1252 491
BsaHI Gr'CG_yC
Cuts at: 0 1433 1463 1592 1743
Size: 1433 30 129 151
Fragments arranged by size:
1433 151 129 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 86 116 1501 1522 1531 1552 1743
Size: 86 30 1385 21 9 21 191
Fragments arranged by size:
1385 191 86 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 175 373 1546 1549 1705 1743
Size: 37 138 198 1173 3 156 38
Fragments arranged by size:
1173 198 156 138 38 37 3
BseYI C'CCAG_C
Cuts at: 0 152 1386 1743
Size: 152 1234 357
Fragments arranged by size:
1234 357 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1627 1743
Size: 154 1473 116
Fragments arranged by size:
1473 154 116
BsiHKAI G_wGCw'C
Cuts at: 0 104 653 1370 1514 1526 1743
Size: 104 549 717 144 12 217
Fragments arranged by size:
717 549 217 144 104 12
Bsp1286I G_dGCh'C
Cuts at: 0 104 653 1322 1370 1514 1526 1708 1743
Size: 104 549 669 48 144 12 182 35
Fragments arranged by size:
669 549 182 144 104 48 35 12
BspHI T'CATG_A
Cuts at: 0 45 1743
Size: 45 1698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1649 1743
Size: 1649 94
BsrBI CCG'CTC
Cuts at: 0 1180 1743
Size: 1180 563
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1737 1743
Size: 1737 6
BstYI r'GATC_y
Cuts at: 0 1031 1743
Size: 1031 712
BtgI C'CryG_G
Cuts at: 0 1058 1322 1647 1743
Size: 1058 264 325 96
Fragments arranged by size:
1058 325 264 96
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 63 98 1743
Size: 63 35 1645
Fragments arranged by size:
1645 63 35
DraI TTT'AAA
Cuts at: 0 327 1743
Size: 327 1416
EaeI y'GGCC_r
Cuts at: 0 608 965 1743
Size: 608 357 778
Fragments arranged by size:
778 608 357
EarI CTCTTCn'nnn_
Cuts at: 0 14 1375 1743
Size: 14 1361 368
Fragments arranged by size:
1361 368 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 31 1743
Size: 31 1712
EcoICRI GAG'CTC
Cuts at: 0 1524 1743
Size: 1524 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 740 877 1336 1743
Size: 174 566 137 459 407
Fragments arranged by size:
566 459 407 174 137
EcoO109I rG'GnC_Cy
Cuts at: 0 1566 1638 1704 1705 1743
Size: 1566 72 66 1 38
Fragments arranged by size:
1566 72 66 38 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 453 1743
Size: 453 1290
HaeII r_GCGC'y
Cuts at: 0 820 1743
Size: 820 923
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 529 561 679 711 1501 1533
Size: 151 32 346 32 118 32 790 32
Cuts at: 1533 1653 1685 1743
Size: 120 32 58
Fragments arranged by size:
790 346 151 120 118 58 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1467 1743
Size: 1467 276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1487 1499 1743
Size: 1487 12 244
Fragments arranged by size:
1487 244 12
MscI TGG'CCA
Cuts at: 0 610 967 1743
Size: 610 357 776
Fragments arranged by size:
776 610 357
MslI CAynn'nnrTG
Cuts at: 0 223 988 1660 1743
Size: 223 765 672 83
Fragments arranged by size:
765 672 223 83
MspA1I CmG'CkG
Cuts at: 0 173 718 1743
Size: 173 545 1025
Fragments arranged by size:
1025 545 173
NcoI C'CATG_G
Cuts at: 0 1322 1743
Size: 1322 421
NspI r_CATG'y
Cuts at: 0 375 660 1082 1743
Size: 375 285 422 661
Fragments arranged by size:
661 422 375 285
PciI A'CATG_T
Cuts at: 0 371 1078 1743
Size: 371 707 665
Fragments arranged by size:
707 665 371
PflMI CCAn_nnn'nTGG
Cuts at: 0 1682 1743
Size: 1682 61
PfoI T'CCnGG_A
Cuts at: 0 1220 1743
Size: 1220 523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 1743
Size: 13 32 1698
Fragments arranged by size:
1698 32 13
PpuMI rG'GwC_Cy
Cuts at: 0 1566 1638 1743
Size: 1566 72 105
Fragments arranged by size:
1566 105 72
PsiI TTA'TAA
Cuts at: 0 310 1743
Size: 310 1433
PspOMI G'GGCC_C
Cuts at: 0 1704 1743
Size: 1704 39
PstI C_TGCA'G
Cuts at: 0 816 1743
Size: 816 927
PvuII CAG'CTG
Cuts at: 0 718 1743
Size: 718 1025
SacI G_AGCT'C
Cuts at: 0 1526 1743
Size: 1526 217
SapI GCTCTTCn'nnn_
Cuts at: 0 1375 1743
Size: 1375 368
SfcI C'TryA_G
Cuts at: 0 812 1743
Size: 812 931
SmlI C'TyrA_G
Cuts at: 0 870 1570 1743
Size: 870 700 173
Fragments arranged by size:
870 700 173
SspI AAT'ATT
Cuts at: 0 450 1743
Size: 450 1293
StyI C'CwwG_G
Cuts at: 0 887 1322 1436 1743
Size: 887 435 114 307
Fragments arranged by size:
887 435 307 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 746 1042 1743
Size: 746 296 701
Fragments arranged by size:
746 701 296
TatI w'GTAC_w
Cuts at: 0 1164 1743
Size: 1164 579
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1383 1743
Size: 1383 360
XmnI GAAnn'nnTTC
Cuts at: 0 359 677 1216 1609 1743
Size: 359 318 539 393 134
Fragments arranged by size:
539 393 359 318 134
ZraI GAC'GTC
Cuts at: 0 1464 1743
Size: 1464 279
Enzymes that do cut:
AatII AcuI AfeI AflII AflIII AlfI AlwNI ApaI
ApoI BanI BanII BbvCI BcgI BclI BglI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BstAPI
BstYI BtgI CspCI DraI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NcoI NspI PciI PflMI PfoI PpiI
PpuMI PsiI PspOMI PstI PvuII SacI SapI SfcI
SmlI SspI StyI TaqII TatI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AgeI AhdI AleI AloI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BciVI BfrBI BglII BlpI BmgBI BmtI
BplI BsaI BsaAI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI DraIII DrdI
EagI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PshAI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XbaI XhoI XmaI