Restriction map for
pF1KSDA0927
(Linear) (Six-base) MAPSORT of: KIAA0927.seq Check: 773 from: 1 to: 2847
KIAA0927.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 360 1484 1786 2365 2847
Size: 360 1124 302 579 482
Fragments arranged by size:
1124 579 482 360 302
AatII G_ACGT'C
Cuts at: 0 2626 2847
Size: 2626 221
AccI GT'mk_AC
Cuts at: 0 941 1106 2847
Size: 941 165 1741
Fragments arranged by size:
1741 941 165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 2847
Size: 206 2641
AfeI AGC'GCT
Cuts at: 0 83 2847
Size: 83 2764
AleI CACnn'nnGTG
Cuts at: 0 1232 2847
Size: 1232 1615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1849 2847
Size: 1849 998
AlwNI CAG_nnn'CTG
Cuts at: 0 421 1470 2016 2115 2847
Size: 421 1049 546 99 732
Fragments arranged by size:
1049 732 546 421 99
ApaI G_GGCC'C
Cuts at: 0 1810 2585 2847
Size: 1810 775 262
Fragments arranged by size:
1810 775 262
ApaLI G'TGCA_C
Cuts at: 0 921 1366 2847
Size: 921 445 1481
Fragments arranged by size:
1481 921 445
AvaI C'yCGr_G
Cuts at: 0 103 661 1646 1933 2245 2847
Size: 103 558 985 287 312 602
Fragments arranged by size:
985 602 558 312 287 103
BanII G_rGCy'C
Cuts at: 0 73 153 965 1274 1337 1709 1810 1865
Size: 73 80 812 309 63 372 101 55
Cuts at: 1865 1895 1940 2528 2585 2847
Size: 30 45 588 57 262
Fragments arranged by size:
812 588 372 309 262 101 80 73
63 57 55 45 30
BbvCI CC'TCA_GC
Cuts at: 0 394 2371 2847
Size: 394 1977 476
Fragments arranged by size:
1977 476 394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 128 2606 2640 2847
Size: 94 34 2478 34 207
Fragments arranged by size:
2478 207 94 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2446 2847
Size: 2446 401
BfrBI ATG'CAT
Cuts at: 0 1298 1826 2847
Size: 1298 528 1021
Fragments arranged by size:
1298 1021 528
BglI GCCn_nnn'nGGC
Cuts at: 0 552 691 1262 1651 2847
Size: 552 139 571 389 1196
Fragments arranged by size:
1196 571 552 389 139
BglII A'GATC_T
Cuts at: 0 462 2847
Size: 462 2385
BlpI GC'TnA_GC
Cuts at: 0 1171 1344 1600 2847
Size: 1171 173 256 1247
Fragments arranged by size:
1247 1171 256 173
Bme1580I G_kGCm'C
Cuts at: 0 548 925 1370 1810 2585 2847
Size: 548 377 445 440 775 262
Fragments arranged by size:
775 548 445 440 377 262
BmgBI CAC'GTC
Cuts at: 0 1634 2847
Size: 1634 1213
BmrI ACTGGGnnnn_n'
Cuts at: 0 206 761 2847
Size: 206 555 2086
Fragments arranged by size:
2086 555 206
BmtI G_CTAG'C
Cuts at: 0 119 2847
Size: 119 2728
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2512 2847
Size: 2512 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 864 937 949 1258 1341 1439 1819
Size: 106 758 73 12 309 83 98 380
Cuts at: 1819 2397 2847
Size: 578 450
Fragments arranged by size:
758 578 450 380 309 106 98 83
73 12
Bpu10I CC'TnA_GC
Cuts at: 0 394 2371 2847
Size: 394 1977 476
Fragments arranged by size:
1977 476 394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 2847
Size: 349 2498
BsaI GGTCTCn'nnnn_
Cuts at: 0 772 2847
Size: 772 2075
BsaAI yAC'GTr
Cuts at: 0 1944 1977 2847
Size: 1944 33 870
Fragments arranged by size:
1944 870 33
BsaHI Gr'CG_yC
Cuts at: 0 2623 2847
Size: 2623 224
BsaWI w'CCGG_w
Cuts at: 0 1112 2847
Size: 1112 1735
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 862 892 1084 1114 1349 1379 2847
Size: 862 30 192 30 235 30 1468
Fragments arranged by size:
1468 862 235 192 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 457 462 465 544 781 2112 2425
Size: 328 129 5 3 79 237 1331 313
Cuts at: 2425 2708 2847
Size: 283 139
Fragments arranged by size:
1331 328 313 283 237 139 129 79
5 3
BseYI C'CCAG_C
Cuts at: 0 237 288 440 1906 2688 2847
Size: 237 51 152 1466 782 159
Fragments arranged by size:
1466 782 237 159 152 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1210 1456 1462 1890 2847
Size: 257 953 246 6 428 957
Fragments arranged by size:
957 953 428 257 246 6
BsiEI CG_ry'CG
Cuts at: 0 16 1386 2847
Size: 16 1370 1461
Fragments arranged by size:
1461 1370 16
BsiHKAI G_wGCw'C
Cuts at: 0 182 925 965 1274 1370 1895 2528 2847
Size: 182 743 40 309 96 525 633 319
Fragments arranged by size:
743 633 525 319 309 182 96 40
BsmI GAATG_Cn'
Cuts at: 0 1828 2847
Size: 1828 1019
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2614 2847
Size: 2614 233
Bsp1286I G_dGCh'C
Cuts at: 0 73 153 182 548 925 965 1274 1337
Size: 73 80 29 366 377 40 309 63
Cuts at: 1337 1370 1709 1810 1865 1895 1940 2528 2585
Size: 33 339 101 55 30 45 588 57
Cuts at: 2585 2847
Size: 262
Fragments arranged by size:
588 377 366 339 309 262 101 80
73 63 57 55 45 40 33 30
29
BspEI T'CCGG_A
Cuts at: 0 1112 2847
Size: 1112 1735
BspHI T'CATG_A
Cuts at: 0 2198 2847
Size: 2198 649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 360 1062 1232 1484 1786 2365 2489 2847
Size: 360 702 170 252 302 579 124 358
Fragments arranged by size:
702 579 360 358 302 252 170 124
BsrFI r'CCGG_y
Cuts at: 0 1190 2847
Size: 1190 1657
BsrGI T'GTAC_A
Cuts at: 0 2465 2847
Size: 2465 382
BstAPI GCAn_nnn'nTGC
Cuts at: 0 271 336 2847
Size: 271 65 2511
Fragments arranged by size:
2511 271 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 717 2573 2847
Size: 717 1856 274
Fragments arranged by size:
1856 717 274
BstYI r'GATC_y
Cuts at: 0 38 462 1970 1994 2249 2847
Size: 38 424 1508 24 255 598
Fragments arranged by size:
1508 598 424 255 38 24
Bsu36I CC'TnA_GG
Cuts at: 0 144 863 2847
Size: 144 719 1984
Fragments arranged by size:
1984 719 144
BtgI C'CryG_G
Cuts at: 0 5 23 33 479 716 806 1011 2780
Size: 5 18 10 446 237 90 205 1769
Cuts at: 2780 2847
Size: 67
Fragments arranged by size:
1769 446 237 205 90 67 18 10
5
BtsI GCAGTG_nn'
Cuts at: 0 849 892 1251 1370 1695 2847
Size: 849 43 359 119 325 1152
Fragments arranged by size:
1152 849 359 325 119 43
DrdI GACnn_nn'nnGTC
Cuts at: 0 1542 2847
Size: 1542 1305
EaeI y'GGCC_r
Cuts at: 0 13 1043 1383 1788 1811 2847
Size: 13 1030 340 405 23 1036
Fragments arranged by size:
1036 1030 405 340 23 13
EagI C'GGCC_G
Cuts at: 0 13 1383 2847
Size: 13 1370 1464
Fragments arranged by size:
1464 1370 13
EarI CTCTTCn'nnn_
Cuts at: 0 104 182 967 1556 1979 2847
Size: 104 78 785 589 423 868
Fragments arranged by size:
868 785 589 423 104 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 2728 2847
Size: 244 2484 119
Fragments arranged by size:
2484 244 119
EcoICRI GAG'CTC
Cuts at: 0 963 1272 1893 2526 2847
Size: 963 309 621 633 321
Fragments arranged by size:
963 633 621 321 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 206 864 937 949 1258 1341 1439
Size: 106 100 658 73 12 309 83 98
Cuts at: 1439 1819 2397 2847
Size: 380 578 450
Fragments arranged by size:
658 578 450 380 309 106 100 98
83 73 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1063 1355 1801 2847
Size: 1063 292 446 1046
Fragments arranged by size:
1063 1046 446 292
EcoO109I rG'GnC_Cy
Cuts at: 0 127 437 456 504 693 1139 1593 1806
Size: 127 310 19 48 189 446 454 213
Cuts at: 1806 1839 2367 2582 2847
Size: 33 528 215 265
Fragments arranged by size:
528 454 446 310 265 215 213 189
127 48 33 19
EcoRV GAT'ATC
Cuts at: 0 2007 2847
Size: 2007 840
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 351 565 2847
Size: 351 214 2282
Fragments arranged by size:
2282 351 214
HaeII r_GCGC'y
Cuts at: 0 85 208 1283 2847
Size: 85 123 1075 1564
Fragments arranged by size:
1564 1075 123 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 150 182 454 486 520 552 1213 1245
Size: 150 32 272 32 34 32 661 32
Cuts at: 1245 1437 1469 1618 1650 2218 2250 2288 2320
Size: 192 32 149 32 568 32 38 32
Cuts at: 2320 2444 2476 2847
Size: 124 32 371
Fragments arranged by size:
661 568 371 272 192 150 149 124
38 34 32 32 32 32 32 32
32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1661 1763 2756 2847
Size: 1661 102 993 91
Fragments arranged by size:
1661 993 102 91
MscI TGG'CCA
Cuts at: 0 1045 2847
Size: 1045 1802
MslI CAynn'nnrTG
Cuts at: 0 1216 1232 1700 1831 2503 2847
Size: 1216 16 468 131 672 344
Fragments arranged by size:
1216 672 468 344 131 16
MspA1I CmG'CkG
Cuts at: 0 7 25 35 303 393 1013 1529 2375
Size: 7 18 10 268 90 620 516 846
Cuts at: 2375 2549 2847
Size: 174 298
Fragments arranged by size:
846 620 516 298 268 174 90 18
10 7
NaeI GCC'GGC
Cuts at: 0 1192 2847
Size: 1192 1655
NcoI C'CATG_G
Cuts at: 0 716 2847
Size: 716 2131
NgoMIV G'CCGG_C
Cuts at: 0 1190 2847
Size: 1190 1657
NheI G'CTAG_C
Cuts at: 0 115 2847
Size: 115 2732
NsiI A_TGCA'T
Cuts at: 0 1300 1828 2847
Size: 1300 528 1019
Fragments arranged by size:
1300 1019 528
NspI r_CATG'y
Cuts at: 0 1298 2426 2847
Size: 1298 1128 421
Fragments arranged by size:
1298 1128 421
PasI CC'CwG_GG
Cuts at: 0 453 2434 2847
Size: 453 1981 413
Fragments arranged by size:
1981 453 413
PflMI CCAn_nnn'nTGG
Cuts at: 0 1797 2079 2847
Size: 1797 282 768
Fragments arranged by size:
1797 768 282
PfoI T'CCnGG_A
Cuts at: 0 1121 2847
Size: 1121 1726
PmlI CAC'GTG
Cuts at: 0 1977 2847
Size: 1977 870
PpuMI rG'GwC_Cy
Cuts at: 0 127 437 504 1139 1839 2367 2847
Size: 127 310 67 635 700 528 480
Fragments arranged by size:
700 635 528 480 310 127 67
PspOMI G'GGCC_C
Cuts at: 0 1806 2581 2847
Size: 1806 775 266
Fragments arranged by size:
1806 775 266
PstI C_TGCA'G
Cuts at: 0 844 2847
Size: 844 2003
PvuII CAG'CTG
Cuts at: 0 393 2375 2847
Size: 393 1982 472
Fragments arranged by size:
1982 472 393
SacI G_AGCT'C
Cuts at: 0 965 1274 1895 2528 2847
Size: 965 309 621 633 319
Fragments arranged by size:
965 633 621 319 309
SacII CC_GC'GG
Cuts at: 0 8 26 36 1014 2847
Size: 8 18 10 978 1833
Fragments arranged by size:
1833 978 18 10 8
SanDI GG'GwC_CC
Cuts at: 0 437 504 1839 2847
Size: 437 67 1335 1008
Fragments arranged by size:
1335 1008 437 67
SapI GCTCTTCn'nnn_
Cuts at: 0 104 1556 2847
Size: 104 1452 1291
Fragments arranged by size:
1452 1291 104
SfcI C'TryA_G
Cuts at: 0 840 1763 2766 2847
Size: 840 923 1003 81
Fragments arranged by size:
1003 923 840 81
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 691 1651 2847
Size: 691 960 1196
Fragments arranged by size:
1196 960 691
SmaI CCC'GGG
Cuts at: 0 663 1648 2847
Size: 663 985 1199
Fragments arranged by size:
1199 985 663
SmlI C'TyrA_G
Cuts at: 0 364 2847
Size: 364 2483
SrfI GCCC'GGGC
Cuts at: 0 1648 2847
Size: 1648 1199
StuI AGG'CCT
Cuts at: 0 1162 2847
Size: 1162 1685
StyI C'CwwG_G
Cuts at: 0 401 716 1134 2091 2847
Size: 401 315 418 957 756
Fragments arranged by size:
957 756 418 401 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2521 2847
Size: 2521 326
TatI w'GTAC_w
Cuts at: 0 2117 2465 2753 2847
Size: 2117 348 288 94
Fragments arranged by size:
2117 348 288 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1349 1381 2847
Size: 1349 32 1466
Fragments arranged by size:
1466 1349 32
Tth111I GACn'n_nGTC
Cuts at: 0 753 2062 2847
Size: 753 1309 785
Fragments arranged by size:
1309 785 753
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 723 1014 2847
Size: 723 291 1833
Fragments arranged by size:
1833 723 291
XmaI C'CCGG_G
Cuts at: 0 661 1646 2847
Size: 661 985 1201
Fragments arranged by size:
1201 985 661
ZraI GAC'GTC
Cuts at: 0 2624 2847
Size: 2624 223
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI AvaI BanII BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrFI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtsI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
NsiI NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SapI SfcI SfiI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbsI BclI BsaBI BsiWI BsrBI BsrDI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
EcoRI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NotI NruI PacI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SbfI ScaI SexAI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI XbaI XhoI XmnI