Restriction map for
pF1KSDA0899
(Linear) (Six-base) MAPSORT of: KIAA0899.seq Check: 2644 from: 1 to: 2817
KIAA0899.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 590 1577 2337 2346 2817
Size: 590 987 760 9 471
Fragments arranged by size:
987 760 590 471 9
AatII G_ACGT'C
Cuts at: 0 535 715 2817
Size: 535 180 2102
Fragments arranged by size:
2102 535 180
AccI GT'mk_AC
Cuts at: 0 1957 2817
Size: 1957 860
Acc65I G'GTAC_C
Cuts at: 0 1382 2817
Size: 1382 1435
AclI AA'CG_TT
Cuts at: 0 2517 2817
Size: 2517 300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 1294 1441 2272 2817
Size: 1147 147 147 831 545
Fragments arranged by size:
1147 831 545 147 147
AflII C'TTAA_G
Cuts at: 0 2179 2817
Size: 2179 638
AflIII A'CryG_T
Cuts at: 0 2057 2441 2817
Size: 2057 384 376
Fragments arranged by size:
2057 384 376
AleI CACnn'nnGTG
Cuts at: 0 602 2817
Size: 602 2215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1748 2062 2817
Size: 1748 314 755
Fragments arranged by size:
1748 755 314
AlwNI CAG_nnn'CTG
Cuts at: 0 817 1008 2817
Size: 817 191 1809
Fragments arranged by size:
1809 817 191
ApaI G_GGCC'C
Cuts at: 0 1861 2020 2817
Size: 1861 159 797
Fragments arranged by size:
1861 797 159
ApaLI G'TGCA_C
Cuts at: 0 577 1056 2817
Size: 577 479 1761
Fragments arranged by size:
1761 577 479
ApoI r'AATT_y
Cuts at: 0 127 2188 2199 2473 2658 2817
Size: 127 2061 11 274 185 159
Fragments arranged by size:
2061 274 185 159 127 11
AvaI C'yCGr_G
Cuts at: 0 569 1539 1990 2817
Size: 569 970 451 827
Fragments arranged by size:
970 827 569 451
BanI G'GyrC_C
Cuts at: 0 1382 2394 2428 2817
Size: 1382 1012 34 389
Fragments arranged by size:
1382 1012 389 34
BanII G_rGCy'C
Cuts at: 0 1861 2020 2817
Size: 1861 159 797
Fragments arranged by size:
1861 797 159
BbeI G_GCGC'C
Cuts at: 0 2398 2817
Size: 2398 419
BbsI GAAGACnn'nnnn_
Cuts at: 0 36 1135 2114 2817
Size: 36 1099 979 703
Fragments arranged by size:
1099 979 703 36
BciVI GTATCCnnnnn_n'
Cuts at: 0 1320 2817
Size: 1320 1497
BclI T'GATC_A
Cuts at: 0 332 629 2817
Size: 332 297 2188
Fragments arranged by size:
2188 332 297
BglI GCCn_nnn'nGGC
Cuts at: 0 1288 2817
Size: 1288 1529
BglII A'GATC_T
Cuts at: 0 726 2817
Size: 726 2091
BlpI GC'TnA_GC
Cuts at: 0 697 1225 1753 2817
Size: 697 528 528 1064
Fragments arranged by size:
1064 697 528 528
Bme1580I G_kGCm'C
Cuts at: 0 581 1060 1861 2020 2431 2817
Size: 581 479 801 159 411 386
Fragments arranged by size:
801 581 479 411 386 159
BmgBI CAC'GTC
Cuts at: 0 1143 1419 1680 1716 1911 2058 2817
Size: 1143 276 261 36 195 147 759
Fragments arranged by size:
1143 759 276 261 195 147 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 868 1066 1255 1553 1813 1900 2817
Size: 715 153 198 189 298 260 87 917
Fragments arranged by size:
917 715 298 260 198 189 153 87
Bpu10I CC'TnA_GC
Cuts at: 0 1702 1925 2817
Size: 1702 223 892
Fragments arranged by size:
1702 892 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1828 2817
Size: 1828 989
BsaI GGTCTCn'nnnn_
Cuts at: 0 844 1015 1529 1993 2817
Size: 844 171 514 464 824
Fragments arranged by size:
844 824 514 464 171
BsaAI yAC'GTr
Cuts at: 0 1365 2817
Size: 1365 1452
BsaBI GATnn'nnATC
Cuts at: 0 1402 2215 2648 2817
Size: 1402 813 433 169
Fragments arranged by size:
1402 813 433 169
BsaHI Gr'CG_yC
Cuts at: 0 532 712 2395 2817
Size: 532 180 1683 422
Fragments arranged by size:
1683 532 422 180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1039 1069 1807 1837 1950 1980 2817
Size: 1039 30 738 30 113 30 837
Fragments arranged by size:
1039 837 738 113 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1162 1810 2019 2817
Size: 1162 648 209 798
Fragments arranged by size:
1162 798 648 209
BseYI C'CCAG_C
Cuts at: 0 1861 1935 1983 2817
Size: 1861 74 48 834
Fragments arranged by size:
1861 834 74 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 1441 1558 1614 2371 2470 2572 2609
Size: 379 1062 117 56 757 99 102 37
Cuts at: 2609 2817
Size: 208
Fragments arranged by size:
1062 757 379 208 117 102 99 56
37
BsiEI CG_ry'CG
Cuts at: 0 8 1191 1787 2330 2817
Size: 8 1183 596 543 487
Fragments arranged by size:
1183 596 543 487 8
BsiHKAI G_wGCw'C
Cuts at: 0 581 1060 2817
Size: 581 479 1757
Fragments arranged by size:
1757 581 479
BsmI GAATG_Cn'
Cuts at: 0 921 2817
Size: 921 1896
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1102 2817
Size: 1102 1715
Bsp1286I G_dGCh'C
Cuts at: 0 581 1060 1861 2020 2431 2817
Size: 581 479 801 159 411 386
Fragments arranged by size:
801 581 479 411 386 159
BspHI T'CATG_A
Cuts at: 0 207 2817
Size: 207 2610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 590 980 1037 1577 1754 1988 2312
Size: 251 339 390 57 540 177 234 324
Cuts at: 2312 2337 2346 2738 2817
Size: 25 9 392 79
Fragments arranged by size:
540 392 390 339 324 251 234 177
79 57 25 9
BsrBI CCG'CTC
Cuts at: 0 1137 1818 2817
Size: 1137 681 999
Fragments arranged by size:
1137 999 681
BsrFI r'CCGG_y
Cuts at: 0 3 2747 2817
Size: 3 2744 70
Fragments arranged by size:
2744 70 3
BsrGI T'GTAC_A
Cuts at: 0 524 2817
Size: 524 2293
BssHII G'CGCG_C
Cuts at: 0 1610 2342 2817
Size: 1610 732 475
Fragments arranged by size:
1610 732 475
BssSI C'ACGA_G
Cuts at: 0 1474 2050 2817
Size: 1474 576 767
Fragments arranged by size:
1474 767 576
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2550 2817
Size: 2550 267
BstXI CCAn_nnnn'nTGG
Cuts at: 0 381 2319 2817
Size: 381 1938 498
Fragments arranged by size:
1938 498 381
BstYI r'GATC_y
Cuts at: 0 449 726 1543 2817
Size: 449 277 817 1274
Fragments arranged by size:
1274 817 449 277
Bsu36I CC'TnA_GG
Cuts at: 0 455 2817
Size: 455 2362
BtgI C'CryG_G
Cuts at: 0 551 1163 1760 2012 2318 2330 2817
Size: 551 612 597 252 306 12 487
Fragments arranged by size:
612 597 551 487 306 252 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 387 2817
Size: 387 2430
BtsI GCAGTG_nn'
Cuts at: 0 396 802 827 2817
Size: 396 406 25 1990
Fragments arranged by size:
1990 406 396 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2682 2717 2817
Size: 2682 35 100
Fragments arranged by size:
2682 100 35
DraI TTT'AAA
Cuts at: 0 132 666 2817
Size: 132 534 2151
Fragments arranged by size:
2151 534 132
DrdI GACnn_nn'nnGTC
Cuts at: 0 2590 2817
Size: 2590 227
EaeI y'GGCC_r
Cuts at: 0 5 359 428 1064 1214 1433 1763 2817
Size: 5 354 69 636 150 219 330 1054
Fragments arranged by size:
1054 636 354 330 219 150 69 5
EagI C'GGCC_G
Cuts at: 0 5 2817
Size: 5 2812
EarI CTCTTCn'nnn_
Cuts at: 0 193 653 1256 1367 1460 1654 2817
Size: 193 460 603 111 93 194 1163
Fragments arranged by size:
1163 603 460 194 193 111 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 316 2817
Size: 316 2501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 868 1066 1147 1255 1294 1441 1553
Size: 715 153 198 81 108 39 147 112
Cuts at: 1553 1813 1900 2272 2817
Size: 260 87 372 545
Fragments arranged by size:
715 545 372 260 198 153 147 112
108 87 81 39
EcoO109I rG'GnC_Cy
Cuts at: 0 33 459 1857 1858 1921 2017 2817
Size: 33 426 1398 1 63 96 800
Fragments arranged by size:
1398 800 426 96 63 33 1
EcoRV GAT'ATC
Cuts at: 0 57 2817
Size: 57 2760
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 204 2817
Size: 204 2613
FspI TGC'GCA
Cuts at: 0 1687 2761 2817
Size: 1687 1074 56
Fragments arranged by size:
1687 1074 56
HaeII r_GCGC'y
Cuts at: 0 1952 2398 2817
Size: 1952 446 419
Fragments arranged by size:
1952 446 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 1528 1560 1807 1839 2047 2059
Size: 550 32 946 32 247 32 208 12
Cuts at: 2059 2079 2091 2362 2394 2660 2692 2817
Size: 20 12 271 32 266 32 125
Fragments arranged by size:
946 550 271 266 247 208 125 32
32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 0 2364 2817
Size: 2364 453
KasI G'GCGC_C
Cuts at: 0 2394 2817
Size: 2394 423
KpnI G_GTAC'C
Cuts at: 0 1386 2817
Size: 1386 1431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 2188 2405 2500 2817
Size: 929 1259 217 95 317
Fragments arranged by size:
1259 929 317 217 95
MscI TGG'CCA
Cuts at: 0 361 1066 1765 2817
Size: 361 705 699 1052
Fragments arranged by size:
1052 705 699 361
MslI CAynn'nnrTG
Cuts at: 0 295 379 602 959 2817
Size: 295 84 223 357 1858
Fragments arranged by size:
1858 357 295 223 84
MspA1I CmG'CkG
Cuts at: 0 1158 1243 1554 2014 2042 2163 2454 2817
Size: 1158 85 311 460 28 121 291 363
Fragments arranged by size:
1158 460 363 311 291 121 85 28
NaeI GCC'GGC
Cuts at: 0 5 2817
Size: 5 2812
NarI GG'CG_CC
Cuts at: 0 2395 2817
Size: 2395 422
NcoI C'CATG_G
Cuts at: 0 551 2817
Size: 551 2266
NgoMIV G'CCGG_C
Cuts at: 0 3 2817
Size: 3 2814
NspI r_CATG'y
Cuts at: 0 1056 2817
Size: 1056 1761
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 2817
Size: 600 2217
PfoI T'CCnGG_A
Cuts at: 0 731 1652 1673 1811 2817
Size: 731 921 21 138 1006
Fragments arranged by size:
1006 921 731 138 21
PpuMI rG'GwC_Cy
Cuts at: 0 459 1921 2817
Size: 459 1462 896
Fragments arranged by size:
1462 896 459
PshAI GACnn'nnGTC
Cuts at: 0 2181 2817
Size: 2181 636
PspOMI G'GGCC_C
Cuts at: 0 1857 2016 2817
Size: 1857 159 801
Fragments arranged by size:
1857 801 159
PstI C_TGCA'G
Cuts at: 0 618 797 822 1013 1727 2309 2817
Size: 618 179 25 191 714 582 508
Fragments arranged by size:
714 618 582 508 191 179 25
PvuII CAG'CTG
Cuts at: 0 1243 2163 2454 2817
Size: 1243 920 291 363
Fragments arranged by size:
1243 920 363 291
SacII CC_GC'GG
Cuts at: 0 2015 2817
Size: 2015 802
SapI GCTCTTCn'nnn_
Cuts at: 0 193 1460 2817
Size: 193 1267 1357
Fragments arranged by size:
1357 1267 193
SexAI A'CCwGG_T
Cuts at: 0 1315 1481 2817
Size: 1315 166 1336
Fragments arranged by size:
1336 1315 166
SfcI C'TryA_G
Cuts at: 0 156 614 793 818 1009 1723 2305 2817
Size: 156 458 179 25 191 714 582 512
Fragments arranged by size:
714 582 512 458 191 179 156 25
SfoI GGC'GCC
Cuts at: 0 2396 2817
Size: 2396 421
SmlI C'TyrA_G
Cuts at: 0 1843 2179 2817
Size: 1843 336 638
Fragments arranged by size:
1843 638 336
StuI AGG'CCT
Cuts at: 0 436 2817
Size: 436 2381
StyI C'CwwG_G
Cuts at: 0 14 201 551 2817
Size: 14 187 350 2266
Fragments arranged by size:
2266 350 187 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 2591 2710 2817
Size: 592 1999 119 107
Fragments arranged by size:
1999 592 119 107
TatI w'GTAC_w
Cuts at: 0 524 2817
Size: 524 2293
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1039 1071 2817
Size: 1039 32 1746
Fragments arranged by size:
1746 1039 32
XmnI GAAnn'nnTTC
Cuts at: 0 1648 2560 2817
Size: 1648 912 257
Fragments arranged by size:
1648 912 257
ZraI GAC'GTC
Cuts at: 0 533 713 2817
Size: 533 180 2104
Fragments arranged by size:
2104 533 180
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PflMI PfoI PpuMI PshAI PspOMI PstI
PvuII SacII SapI SexAI SfcI SfoI SmlI StuI
StyI TaqII TatI TstI XmnI ZraI
Enzymes that do not cut:
AfeI AgeI AhdI AloI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BfrBI BmrI BmtI BplI
BsaWI BsiWI BspEI BsrDI BstBI BstEII BstZ17I ClaI
DraIII EcoICRI EcoNI EcoRI FseI FspAI HindIII HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SacI SalI SanDI SbfI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XcmI XhoI XmaI