Restriction map for
pF1KSDA0845
(Linear) (Six-base) MAPSORT of: KIAA0845.seq Check: 7334 from: 1 to: 3078
KIAA0845.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 527 3078
Size: 527 2551
AatII G_ACGT'C
Cuts at: 0 160 805 3078
Size: 160 645 2273
Fragments arranged by size:
2273 645 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1421 1433 2446 2729 3078
Size: 808 613 12 1013 283 349
Fragments arranged by size:
1013 808 613 349 283 12
AfeI AGC'GCT
Cuts at: 0 1036 3078
Size: 1036 2042
AhdI GACnn_n'nnGTC
Cuts at: 0 801 897 3078
Size: 801 96 2181
Fragments arranged by size:
2181 801 96
AlwNI CAG_nnn'CTG
Cuts at: 0 309 666 1098 1161 3078
Size: 309 357 432 63 1917
Fragments arranged by size:
1917 432 357 309 63
ApoI r'AATT_y
Cuts at: 0 1283 2356 3078
Size: 1283 1073 722
Fragments arranged by size:
1283 1073 722
AvaI C'yCGr_G
Cuts at: 0 523 1741 2215 3078
Size: 523 1218 474 863
Fragments arranged by size:
1218 863 523 474
BanI G'GyrC_C
Cuts at: 0 34 205 692 745 2607 3078
Size: 34 171 487 53 1862 471
Fragments arranged by size:
1862 487 471 171 53 34
BanII G_rGCy'C
Cuts at: 0 169 742 2531 3078
Size: 169 573 1789 547
Fragments arranged by size:
1789 573 547 169
BbeI G_GCGC'C
Cuts at: 0 38 209 696 749 3078
Size: 38 171 487 53 2329
Fragments arranged by size:
2329 487 171 53 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 2777 3062 3078
Size: 2777 285 16
Fragments arranged by size:
2777 285 16
BbvCI CC'TCA_GC
Cuts at: 0 1111 1583 1727 1787 1847 1889 3078
Size: 1111 472 144 60 60 42 1189
Fragments arranged by size:
1189 1111 472 144 60 60 42
BglI GCCn_nnn'nGGC
Cuts at: 0 377 589 631 754 3078
Size: 377 212 42 123 2324
Fragments arranged by size:
2324 377 212 123 42
BlpI GC'TnA_GC
Cuts at: 0 238 936 1106 3078
Size: 238 698 170 1972
Fragments arranged by size:
1972 698 238 170
BmgBI CAC'GTC
Cuts at: 0 798 3078
Size: 798 2280
BmrI ACTGGGnnnn_n'
Cuts at: 0 1813 1855 2017 2059 2101 2143 2185 2227
Size: 1813 42 162 42 42 42 42 42
Cuts at: 2227 2479 3078
Size: 252 599
Fragments arranged by size:
1813 599 252 162 42 42 42 42
42 42
BmtI G_CTAG'C
Cuts at: 0 85 3078
Size: 85 2993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 373 394 526 1015 1045 1066 1640
Size: 114 259 21 132 489 30 21 574
Cuts at: 1640 1706 1784 1868 2291 2306 2994 3023 3078
Size: 66 78 84 423 15 688 29 55
Fragments arranged by size:
688 574 489 423 259 132 114 84
78 66 55 30 29 21 21 15
Bpu10I CC'TnA_GC
Cuts at: 0 1111 1583 1727 1787 1847 1889 3078
Size: 1111 472 144 60 60 42 1189
Fragments arranged by size:
1189 1111 472 144 60 60 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 3078
Size: 198 2880
BsaI GGTCTCn'nnnn_
Cuts at: 0 1381 3078
Size: 1381 1697
BsaAI yAC'GTr
Cuts at: 0 540 3078
Size: 540 2538
BsaHI Gr'CG_yC
Cuts at: 0 35 157 206 693 746 782 802 966
Size: 35 122 49 487 53 36 20 164
Cuts at: 966 3078
Size: 2112
Fragments arranged by size:
2112 487 164 122 53 49 36 35
20
BsaWI w'CCGG_w
Cuts at: 0 2871 3078
Size: 2871 207
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1057 1087 1491 1521 2383 2413 3078
Size: 1057 30 404 30 862 30 665
Fragments arranged by size:
1057 862 665 404 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 117 592 688 883 961 1399 1435 1453
Size: 117 475 96 195 78 438 36 18
Cuts at: 1453 1456 1489 2404 2509 2512 2593 2617 2656
Size: 3 33 915 105 3 81 24 39
Cuts at: 2656 2908 2929 3078
Size: 252 21 149
Fragments arranged by size:
915 475 438 252 195 149 117 105
96 81 78 39 36 33 24 21
18 3 3
BseYI C'CCAG_C
Cuts at: 0 30 184 1139 1512 1620 1758 2839 3078
Size: 30 154 955 373 108 138 1081 239
Fragments arranged by size:
1081 955 373 239 154 138 108 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 3078
Size: 868 2210
BsiEI CG_ry'CG
Cuts at: 0 318 3078
Size: 318 2760
BsiHKAI G_wGCw'C
Cuts at: 0 169 518 856 3078
Size: 169 349 338 2222
Fragments arranged by size:
2222 349 338 169
BsmBI CGTCTCn'nnnn_
Cuts at: 0 766 3078
Size: 766 2312
Bsp1286I G_dGCh'C
Cuts at: 0 169 518 742 856 2531 3078
Size: 169 349 224 114 1675 547
Fragments arranged by size:
1675 547 349 224 169 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 527 653 695 1169 2664 3078
Size: 527 126 42 474 1495 414
Fragments arranged by size:
1495 527 474 414 126 42
BsrBI CCG'CTC
Cuts at: 0 416 3078
Size: 416 2662
BsrDI GCAATG_nn'
Cuts at: 0 1067 3078
Size: 1067 2011
BsrFI r'CCGG_y
Cuts at: 0 962 1578 1782 1842 3078
Size: 962 616 204 60 1236
Fragments arranged by size:
1236 962 616 204 60
BssHII G'CGCG_C
Cuts at: 0 487 599 607 634 824 3078
Size: 487 112 8 27 190 2254
Fragments arranged by size:
2254 487 190 112 27 8
BssSI C'ACGA_G
Cuts at: 0 144 3078
Size: 144 2934
BstAPI GCAn_nnn'nTGC
Cuts at: 0 666 3078
Size: 666 2412
BstXI CCAn_nnnn'nTGG
Cuts at: 0 993 3078
Size: 993 2085
BstYI r'GATC_y
Cuts at: 0 731 2456 3078
Size: 731 1725 622
Fragments arranged by size:
1725 731 622
Bsu36I CC'TnA_GG
Cuts at: 0 1685 2912 2984 3078
Size: 1685 1227 72 94
Fragments arranged by size:
1685 1227 94 72
BtgI C'CryG_G
Cuts at: 0 195 3078
Size: 195 2883
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 516 3078
Size: 516 2562
BtsI GCAGTG_nn'
Cuts at: 0 291 3078
Size: 291 2787
DraIII CAC_nnn'GTG
Cuts at: 0 286 846 1124 3078
Size: 286 560 278 1954
Fragments arranged by size:
1954 560 286 278
DrdI GACnn_nn'nnGTC
Cuts at: 0 156 3078
Size: 156 2922
EaeI y'GGCC_r
Cuts at: 0 272 726 1133 2801 3078
Size: 272 454 407 1668 277
Fragments arranged by size:
1668 454 407 277 272
EarI CTCTTCn'nnn_
Cuts at: 0 701 1220 1316 1412 1466 1514 1559 1661
Size: 701 519 96 96 54 48 45 102
Cuts at: 1661 1763 1985 2465 2543 2696 3078
Size: 102 222 480 78 153 382
Fragments arranged by size:
701 519 480 382 222 153 102 102
96 96 78 54 48 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 105 165 3078
Size: 105 60 2913
Fragments arranged by size:
2913 105 60
EcoICRI GAG'CTC
Cuts at: 0 167 3078
Size: 167 2911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 373 394 526 808 1015 1045 1066
Size: 114 259 21 132 282 207 30 21
Cuts at: 1066 1421 1433 1640 1706 1784 1868 2291 2306
Size: 355 12 207 66 78 84 423 15
Cuts at: 2306 2446 2729 2994 3023 3078
Size: 140 283 265 29 55
Fragments arranged by size:
423 355 283 282 265 259 207 207
140 132 114 84 78 66 55 30
29 21 21 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 508 523 2428 3078
Size: 508 15 1905 650
Fragments arranged by size:
1905 650 508 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1158 2342 2442 3078
Size: 1158 1184 100 636
Fragments arranged by size:
1184 1158 636 100
HaeII r_GCGC'y
Cuts at: 0 38 209 310 394 552 667 696 749
Size: 38 171 101 84 158 115 29 53
Cuts at: 749 811 1038 3078
Size: 62 227 2040
Fragments arranged by size:
2040 227 171 158 115 101 84 62
53 38 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 787 819 883 915 1057 1089 1513 1545
Size: 787 32 64 32 142 32 424 32
Cuts at: 1545 1558 1576 1590 1608 1660 1678 1692 1710
Size: 13 18 14 18 52 18 14 18
Cuts at: 1710 1720 1752 1762 1780 1794 1812 1840 1872
Size: 10 32 10 18 14 18 28 32
Cuts at: 1872 1882 1914 1984 2016 2287 2319 2383 2415
Size: 10 32 70 32 271 32 64 32
Cuts at: 2415 2464 2496 3078
Size: 49 32 582
Fragments arranged by size:
787 582 424 271 142 70 64 64
52 49 32 32 32 32 32 32
32 32 32 32 32 28 18 18
18 18 18 18 14 14 14 13
10 10 10
KasI G'GCGC_C
Cuts at: 0 34 205 692 745 3078
Size: 34 171 487 53 2333
Fragments arranged by size:
2333 487 171 53 34
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1213 1945 3078
Size: 881 332 732 1133
Fragments arranged by size:
1133 881 732 332
MscI TGG'CCA
Cuts at: 0 274 2803 3078
Size: 274 2529 275
Fragments arranged by size:
2529 275 274
MslI CAynn'nnrTG
Cuts at: 0 1309 3078
Size: 1309 1769
MspA1I CmG'CkG
Cuts at: 0 51 121 134 300 351 972 1098 1143
Size: 51 70 13 166 51 621 126 45
Cuts at: 1143 1726 1888 3078
Size: 583 162 1190
Fragments arranged by size:
1190 621 583 166 162 126 70 51
51 45 13
NarI GG'CG_CC
Cuts at: 0 35 206 693 746 3078
Size: 35 171 487 53 2332
Fragments arranged by size:
2332 487 171 53 35
NheI G'CTAG_C
Cuts at: 0 81 3078
Size: 81 2997
PfoI T'CCnGG_A
Cuts at: 0 1214 3078
Size: 1214 1864
PmlI CAC'GTG
Cuts at: 0 540 3078
Size: 540 2538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 859 891 3078
Size: 859 32 2187
Fragments arranged by size:
2187 859 32
PpuMI rG'GwC_Cy
Cuts at: 0 1158 2342 3078
Size: 1158 1184 736
Fragments arranged by size:
1184 1158 736
PspXI vC'TCGA_Gb
Cuts at: 0 523 3078
Size: 523 2555
PstI C_TGCA'G
Cuts at: 0 305 650 671 863 977 2352 3078
Size: 305 345 21 192 114 1375 726
Fragments arranged by size:
1375 726 345 305 192 114 21
PvuII CAG'CTG
Cuts at: 0 300 351 972 1098 1143 1726 1888 3078
Size: 300 51 621 126 45 583 162 1190
Fragments arranged by size:
1190 621 583 300 162 126 51 45
SacI G_AGCT'C
Cuts at: 0 169 3078
Size: 169 2909
SfcI C'TryA_G
Cuts at: 0 301 646 667 859 973 2348 3078
Size: 301 345 21 192 114 1375 730
Fragments arranged by size:
1375 730 345 301 192 114 21
SfoI GGC'GCC
Cuts at: 0 36 207 694 747 3078
Size: 36 171 487 53 2331
Fragments arranged by size:
2331 487 171 53 36
SmlI C'TyrA_G
Cuts at: 0 213 523 3078
Size: 213 310 2555
Fragments arranged by size:
2555 310 213
StyI C'CwwG_G
Cuts at: 0 911 1013 2438 2633 2753 2825 2831 2888
Size: 911 102 1425 195 120 72 6 57
Cuts at: 2888 2942 3068 3078
Size: 54 126 10
Fragments arranged by size:
1425 911 195 126 120 102 72 57
54 10 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 908 2625 3078
Size: 908 1717 453
Fragments arranged by size:
1717 908 453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1020 1095 1128 3078
Size: 1020 75 33 1950
Fragments arranged by size:
1950 1020 75 33
XhoI C'TCGA_G
Cuts at: 0 523 3078
Size: 523 2555
XmnI GAAnn'nnTTC
Cuts at: 0 1088 3078
Size: 1088 1990
ZraI GAC'GTC
Cuts at: 0 158 803 3078
Size: 158 645 2275
Fragments arranged by size:
2275 645 158
Enzymes that do cut:
AarI AatII AcuI AfeI AhdI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BglI BlpI BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MmeI MscI MslI MspA1I NarI NheI
PfoI PmlI PpiI PpuMI PspXI PstI PvuII SacI
SfcI SfoI SmlI StyI TaqII XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglII Bme1580I BplI
BsaBI BsiWI BsmI BspEI BspHI BsrGI BstBI BstEII
BstZ17I ClaI CspCI DraI EagI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XmaI