Restriction map for
pF1KSDA0833
(Linear) (Six-base) MAPSORT of: KIAA0833.seq Check: 5182 from: 1 to: 5019
KIAA0833.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3503 5019
Size: 3503 1516
AccI GT'mk_AC
Cuts at: 0 3991 5019
Size: 3991 1028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1548 1740 1779 2289 3870 5019
Size: 490 1058 192 39 510 1581 1149
Fragments arranged by size:
1581 1149 1058 510 490 192 39
AflIII A'CryG_T
Cuts at: 0 920 1376 1784 5019
Size: 920 456 408 3235
Fragments arranged by size:
3235 920 456 408
AhdI GACnn_n'nnGTC
Cuts at: 0 1785 2901 5019
Size: 1785 1116 2118
Fragments arranged by size:
2118 1785 1116
AleI CACnn'nnGTG
Cuts at: 0 1138 1375 1924 5019
Size: 1138 237 549 3095
Fragments arranged by size:
3095 1138 549 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2236 2472 5019
Size: 2236 236 2547
Fragments arranged by size:
2547 2236 236
AlwNI CAG_nnn'CTG
Cuts at: 0 1970 2734 3919 4288 5019
Size: 1970 764 1185 369 731
Fragments arranged by size:
1970 1185 764 731 369
ApaI G_GGCC'C
Cuts at: 0 2436 5019
Size: 2436 2583
ApaLI G'TGCA_C
Cuts at: 0 529 5019
Size: 529 4490
ApoI r'AATT_y
Cuts at: 0 3963 4245 4489 4738 4765 5019
Size: 3963 282 244 249 27 254
Fragments arranged by size:
3963 282 254 249 244 27
AvaI C'yCGr_G
Cuts at: 0 1549 1839 2208 2861 3850 5019
Size: 1549 290 369 653 989 1169
Fragments arranged by size:
1549 1169 989 653 369 290
AvrII C'CTAG_G
Cuts at: 0 2580 3357 5019
Size: 2580 777 1662
Fragments arranged by size:
2580 1662 777
BanI G'GyrC_C
Cuts at: 0 212 1918 2375 2575 5019
Size: 212 1706 457 200 2444
Fragments arranged by size:
2444 1706 457 212 200
BanII G_rGCy'C
Cuts at: 0 641 961 1072 1097 1309 2279 2436 2868
Size: 641 320 111 25 212 970 157 432
Cuts at: 2868 3563 4420 5019
Size: 695 857 599
Fragments arranged by size:
970 857 695 641 599 432 320 212
157 111 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 3672 5019
Size: 3672 1347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 150 184 5019
Size: 150 34 4835
Fragments arranged by size:
4835 150 34
BclI T'GATC_A
Cuts at: 0 117 2327 4551 5019
Size: 117 2210 2224 468
Fragments arranged by size:
2224 2210 468 117
BglI GCCn_nnn'nGGC
Cuts at: 0 1805 2053 2566 3151 4424 5019
Size: 1805 248 513 585 1273 595
Fragments arranged by size:
1805 1273 595 585 513 248
BglII A'GATC_T
Cuts at: 0 2723 3972 5019
Size: 2723 1249 1047
Fragments arranged by size:
2723 1249 1047
BlpI GC'TnA_GC
Cuts at: 0 2278 2497 3515 4535 5019
Size: 2278 219 1018 1020 484
Fragments arranged by size:
2278 1020 1018 484 219
Bme1580I G_kGCm'C
Cuts at: 0 533 1921 2378 2436 2578 3357 3365 4256
Size: 533 1388 457 58 142 779 8 891
Cuts at: 4256 5019
Size: 763
Fragments arranged by size:
1388 891 779 763 533 457 142 58
8
BmgBI CAC'GTC
Cuts at: 0 921 1787 2541 5019
Size: 921 866 754 2478
Fragments arranged by size:
2478 921 866 754
BmrI ACTGGGnnnn_n'
Cuts at: 0 3089 3579 3860 4346 5019
Size: 3089 490 281 486 673
Fragments arranged by size:
3089 673 490 486 281
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1047 1626 2571 3686 5019
Size: 1047 579 945 1115 1333
Fragments arranged by size:
1333 1115 1047 945 579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1680 1762 2968 3508 3596 3896 5019
Size: 945 735 82 1206 540 88 300 1123
Fragments arranged by size:
1206 1123 945 735 540 300 88 82
Bpu10I CC'TnA_GC
Cuts at: 0 705 5019
Size: 705 4314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 2098 4434 5019
Size: 403 1695 2336 585
Fragments arranged by size:
2336 1695 585 403
BsaI GGTCTCn'nnnn_
Cuts at: 0 1032 2071 2158 2559 5019
Size: 1032 1039 87 401 2460
Fragments arranged by size:
2460 1039 1032 401 87
BsaAI yAC'GTr
Cuts at: 0 1983 5019
Size: 1983 3036
BsaBI GATnn'nnATC
Cuts at: 0 2932 5019
Size: 2932 2087
BsaHI Gr'CG_yC
Cuts at: 0 1277 1859 2195 5019
Size: 1277 582 336 2824
Fragments arranged by size:
2824 1277 582 336
BsaWI w'CCGG_w
Cuts at: 0 485 1144 4604 5019
Size: 485 659 3460 415
Fragments arranged by size:
3460 659 485 415
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 427 457 1633 1663 1939 1969 2302 2326
Size: 427 30 1176 30 276 30 333 24
Cuts at: 2326 2332 2356 2648 2678 3310 3340 3620 3650
Size: 6 24 292 30 632 30 280 30
Cuts at: 3650 4453 4483 5019
Size: 803 30 536
Fragments arranged by size:
1176 803 632 536 427 333 292 280
276 30 30 30 30 30 30 30
24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1020 1255 1635 1638 2669 2976 3326 5019
Size: 1020 235 380 3 1031 307 350 1693
Fragments arranged by size:
1693 1031 1020 380 350 307 235 3
BseYI C'CCAG_C
Cuts at: 0 764 1729 2243 2248 2477 2775 2888 2987
Size: 764 965 514 5 229 298 113 99
Cuts at: 2987 3149 3519 3562 4119 5019
Size: 162 370 43 557 900
Fragments arranged by size:
965 900 764 557 514 370 298 229
162 113 99 43 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 774 928 991 2242 3773 4160 5019
Size: 751 23 154 63 1251 1531 387 859
Fragments arranged by size:
1531 1251 859 751 387 154 63 23
BsiEI CG_ry'CG
Cuts at: 0 2561 5019
Size: 2561 2458
BsiHKAI G_wGCw'C
Cuts at: 0 533 641 944 1004 1072 1309 2150 2279
Size: 533 108 303 60 68 237 841 129
Cuts at: 2279 3494 4796 5019
Size: 1215 1302 223
Fragments arranged by size:
1302 1215 841 533 303 237 223 129
108 68 60
BsiWI C'GTAC_G
Cuts at: 0 1490 5019
Size: 1490 3529
BsmI GAATG_Cn'
Cuts at: 0 4159 5019
Size: 4159 860
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1981 5019
Size: 1981 3038
Bsp1286I G_dGCh'C
Cuts at: 0 533 641 944 961 1004 1072 1097 1309
Size: 533 108 303 17 43 68 25 212
Cuts at: 1309 1921 2150 2279 2378 2436 2578 2868 3357
Size: 612 229 129 99 58 142 290 489
Cuts at: 3357 3365 3494 3563 4256 4420 4796 5019
Size: 8 129 69 693 164 376 223
Fragments arranged by size:
693 612 533 489 376 303 290 229
223 212 164 142 129 129 108 99
69 68 58 43 25 17 8
BspEI T'CCGG_A
Cuts at: 0 4604 5019
Size: 4604 415
BspHI T'CATG_A
Cuts at: 0 4554 5019
Size: 4554 465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 691 2162 3503 5019
Size: 691 1471 1341 1516
Fragments arranged by size:
1516 1471 1341 691
BsrBI CCG'CTC
Cuts at: 0 1557 2600 3008 3041 5019
Size: 1557 1043 408 33 1978
Fragments arranged by size:
1978 1557 1043 408 33
BsrDI GCAATG_nn'
Cuts at: 0 696 5019
Size: 696 4323
BsrFI r'CCGG_y
Cuts at: 0 546 793 1690 2188 5019
Size: 546 247 897 498 2831
Fragments arranged by size:
2831 897 546 498 247
BsrGI T'GTAC_A
Cuts at: 0 1226 3386 4866 4979 5019
Size: 1226 2160 1480 113 40
Fragments arranged by size:
2160 1480 1226 113 40
BssHII G'CGCG_C
Cuts at: 0 6 5019
Size: 6 5013
BssSI C'ACGA_G
Cuts at: 0 937 5019
Size: 937 4082
BstAPI GCAn_nnn'nTGC
Cuts at: 0 572 2154 3067 5019
Size: 572 1582 913 1952
Fragments arranged by size:
1952 1582 913 572
BstEII G'GTnAC_C
Cuts at: 0 1209 2067 2616 5019
Size: 1209 858 549 2403
Fragments arranged by size:
2403 1209 858 549
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1044 2526 2788 2841 5019
Size: 1044 1482 262 53 2178
Fragments arranged by size:
2178 1482 1044 262 53
BstYI r'GATC_y
Cuts at: 0 737 2723 3940 3972 4364 5019
Size: 737 1986 1217 32 392 655
Fragments arranged by size:
1986 1217 737 655 392 32
Bsu36I CC'TnA_GG
Cuts at: 0 4266 5019
Size: 4266 753
BtgI C'CryG_G
Cuts at: 0 666 769 874 1190 1337 1652 1934 2072
Size: 666 103 105 316 147 315 282 138
Cuts at: 2072 2237 2345 2525 2680 3189 4063 4166 4256
Size: 165 108 180 155 509 874 103 90
Cuts at: 4256 4475 5019
Size: 219 544
Fragments arranged by size:
874 666 544 509 316 315 282 219
180 165 155 147 138 108 105 103
103 90
BtsI GCAGTG_nn'
Cuts at: 0 3027 3705 3785 5019
Size: 3027 678 80 1234
Fragments arranged by size:
3027 1234 678 80
DraIII CAC_nnn'GTG
Cuts at: 0 3067 5019
Size: 3067 1952
EaeI y'GGCC_r
Cuts at: 0 548 989 1298 1314 1416 1628 2558 2969
Size: 548 441 309 16 102 212 930 411
Cuts at: 2969 3212 4158 4319 5019
Size: 243 946 161 700
Fragments arranged by size:
946 930 700 548 441 411 309 243
212 161 102 16
EagI C'GGCC_G
Cuts at: 0 2558 5019
Size: 2558 2461
EarI CTCTTCn'nnn_
Cuts at: 0 37 629 967 1379 3551 3797 5019
Size: 37 592 338 412 2172 246 1222
Fragments arranged by size:
2172 1222 592 412 338 246 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2107 4461 5019
Size: 2107 2354 558
Fragments arranged by size:
2354 2107 558
EcoICRI GAG'CTC
Cuts at: 0 639 1070 1307 2277 5019
Size: 639 431 237 970 2742
Fragments arranged by size:
2742 970 639 431 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 945 1548 1680 1740 1762 1779 2289
Size: 490 455 603 132 60 22 17 510
Cuts at: 2289 2968 3508 3596 3870 3896 5019
Size: 679 540 88 274 26 1123
Fragments arranged by size:
1123 679 603 540 510 490 455 274
132 88 60 26 22 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1585 5019
Size: 1585 3434
EcoO109I rG'GnC_Cy
Cuts at: 0 1287 2106 2433 2664 2984 3083 4124 5019
Size: 1287 819 327 231 320 99 1041 895
Fragments arranged by size:
1287 1041 895 819 327 320 231 99
EcoRI G'AATT_C
Cuts at: 0 3963 4489 5019
Size: 3963 526 530
Fragments arranged by size:
3963 530 526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1344 3455 5019
Size: 1344 2111 1564
Fragments arranged by size:
2111 1564 1344
HaeII r_GCGC'y
Cuts at: 0 842 1190 1763 2189 2407 5019
Size: 842 348 573 426 218 2612
Fragments arranged by size:
2612 842 573 426 348 218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1342 1374 1771 1803 1939 1971 2326 2358
Size: 1342 32 397 32 136 32 355 32
Cuts at: 2358 2648 2680 2710 2742 2887 2919 3310 3342
Size: 290 32 30 32 145 32 391 32
Cuts at: 3342 3996 4028 4108 4140 5019
Size: 654 32 80 32 879
Fragments arranged by size:
1342 879 654 397 391 355 290 145
136 80 32 32 32 32 32 32
32 32 32 32 30
HincII GTy'rAC
Cuts at: 0 3303 5019
Size: 3303 1716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1553 1796 2291 2345 2858 2888 5019
Size: 905 648 243 495 54 513 30 2131
Fragments arranged by size:
2131 905 648 513 495 243 54 30
MscI TGG'CCA
Cuts at: 0 991 1316 1630 4321 5019
Size: 991 325 314 2691 698
Fragments arranged by size:
2691 991 698 325 314
MslI CAynn'nnrTG
Cuts at: 0 1138 1375 1924 2344 3985 4180 5019
Size: 1138 237 549 420 1641 195 839
Fragments arranged by size:
1641 1138 839 549 420 237 195
MspA1I CmG'CkG
Cuts at: 0 218 651 726 771 1091 1246 1654 1976
Size: 218 433 75 45 320 155 408 322
Cuts at: 1976 2382 2496 2708 3035 3191 3217 3519 4477
Size: 406 114 212 327 156 26 302 958
Cuts at: 4477 4946 5019
Size: 469 73
Fragments arranged by size:
958 469 433 408 406 327 322 320
302 218 212 156 155 114 75 73
45 26
NaeI GCC'GGC
Cuts at: 0 548 1692 2190 5019
Size: 548 1144 498 2829
Fragments arranged by size:
2829 1144 548 498
NcoI C'CATG_G
Cuts at: 0 666 2072 2525 4166 4256 5019
Size: 666 1406 453 1641 90 763
Fragments arranged by size:
1641 1406 763 666 453 90
NgoMIV G'CCGG_C
Cuts at: 0 546 1690 2188 5019
Size: 546 1144 498 2831
Fragments arranged by size:
2831 1144 546 498
NspI r_CATG'y
Cuts at: 0 2538 2589 5019
Size: 2538 51 2430
Fragments arranged by size:
2538 2430 51
PasI CC'CwG_GG
Cuts at: 0 2571 3222 5019
Size: 2571 651 1797
Fragments arranged by size:
2571 1797 651
PflMI CCAn_nnn'nTGG
Cuts at: 0 310 432 4167 5019
Size: 310 122 3735 852
Fragments arranged by size:
3735 852 310 122
PfoI T'CCnGG_A
Cuts at: 0 2936 5019
Size: 2936 2083
PmlI CAC'GTG
Cuts at: 0 1983 5019
Size: 1983 3036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 688 720 5019
Size: 688 32 4299
Fragments arranged by size:
4299 688 32
PpuMI rG'GwC_Cy
Cuts at: 0 2106 2664 2984 4124 5019
Size: 2106 558 320 1140 895
Fragments arranged by size:
2106 1140 895 558 320
PshAI GACnn'nnGTC
Cuts at: 0 2789 5019
Size: 2789 2230
PsiI TTA'TAA
Cuts at: 0 4700 4820 5019
Size: 4700 120 199
Fragments arranged by size:
4700 199 120
PspOMI G'GGCC_C
Cuts at: 0 2432 5019
Size: 2432 2587
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3736 3768 5019
Size: 3736 32 1251
Fragments arranged by size:
3736 1251 32
PstI C_TGCA'G
Cuts at: 0 688 1856 1975 2132 2159 2495 2594 3500
Size: 688 1168 119 157 27 336 99 906
Cuts at: 3500 3924 5019
Size: 424 1095
Fragments arranged by size:
1168 1095 906 688 424 336 157 119
99 27
PvuII CAG'CTG
Cuts at: 0 651 726 2382 2496 2708 3519 5019
Size: 651 75 1656 114 212 811 1500
Fragments arranged by size:
1656 1500 811 651 212 114 75
SacI G_AGCT'C
Cuts at: 0 641 1072 1309 2279 5019
Size: 641 431 237 970 2740
Fragments arranged by size:
2740 970 641 431 237
SacII CC_GC'GG
Cuts at: 0 772 1655 3192 4478 5019
Size: 772 883 1537 1286 541
Fragments arranged by size:
1537 1286 883 772 541
SanDI GG'GwC_CC
Cuts at: 0 2984 5019
Size: 2984 2035
SapI GCTCTTCn'nnn_
Cuts at: 0 37 629 1379 3551 3797 5019
Size: 37 592 750 2172 246 1222
Fragments arranged by size:
2172 1222 750 592 246 37
SbfI CC_TGCA'GG
Cuts at: 0 1856 5019
Size: 1856 3163
ScaI AGT'ACT
Cuts at: 0 3754 4807 5019
Size: 3754 1053 212
Fragments arranged by size:
3754 1053 212
SexAI A'CCwGG_T
Cuts at: 0 1040 5019
Size: 1040 3979
SfcI C'TryA_G
Cuts at: 0 107 684 1852 1971 2128 2155 2469 2491
Size: 107 577 1168 119 157 27 314 22
Cuts at: 2491 2590 2703 3496 3693 3920 3950 3992 5019
Size: 99 113 793 197 227 30 42 1027
Fragments arranged by size:
1168 1027 793 577 314 227 197 157
119 113 107 99 42 30 27 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2566 5019
Size: 2566 2453
SgrAI Cr'CCGG_yG
Cuts at: 0 2188 5019
Size: 2188 2831
SmaI CCC'GGG
Cuts at: 0 1551 2210 2863 5019
Size: 1551 659 653 2156
Fragments arranged by size:
2156 1551 659 653
SmlI C'TyrA_G
Cuts at: 0 418 2113 4413 5019
Size: 418 1695 2300 606
Fragments arranged by size:
2300 1695 606 418
SphI G_CATG'C
Cuts at: 0 2589 5019
Size: 2589 2430
SrfI GCCC'GGGC
Cuts at: 0 2863 5019
Size: 2863 2156
StuI AGG'CCT
Cuts at: 0 1505 1951 5019
Size: 1505 446 3068
Fragments arranged by size:
3068 1505 446
StyI C'CwwG_G
Cuts at: 0 666 863 1586 2072 2525 2580 3086 3357
Size: 666 197 723 486 453 55 506 271
Cuts at: 3357 3623 4166 4196 4256 5010 5019
Size: 266 543 30 60 754 9
Fragments arranged by size:
754 723 666 543 506 486 453 271
266 197 60 55 30 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1078 1615 1906 2458 2635 4244 5019
Size: 1078 537 291 552 177 1609 775
Fragments arranged by size:
1609 1078 775 552 537 291 177
TatI w'GTAC_w
Cuts at: 0 1226 2852 3331 3386 3752 3996 4303 4805
Size: 1226 1626 479 55 366 244 307 502
Cuts at: 4805 4866 4979 5019
Size: 61 113 40
Fragments arranged by size:
1626 1226 502 479 366 307 244 113
61 55 40
Tth111I GACn'n_nGTC
Cuts at: 0 520 2982 5019
Size: 520 2462 2037
Fragments arranged by size:
2462 2037 520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1041 1737 5019
Size: 1041 696 3282
Fragments arranged by size:
3282 1041 696
XmaI C'CCGG_G
Cuts at: 0 1549 2208 2861 5019
Size: 1549 659 653 2158
Fragments arranged by size:
2158 1549 659 653
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BanI BanII BbsI
BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuII SacI SacII SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgrAI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI AscI
AseI AsiSI BaeI BamHI BbeI BbvCI BciVI BfrBI
BmtI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PspXI PvuI RsrII SalI SfoI
SgfI SnaBI SpeI SspI SwaI TstI XbaI XhoI
XmnI ZraI